The Impact of Surface Drug Distribution on the Acoustic Behavior of DOX-Loaded Microbubbles

Previous studies have reported substantial improvement of microbubble (MB)-mediated drug delivery with ultrasound when drugs are loaded onto the MB shell compared with a physical mixture. However, drug loading may affect shell properties that determine the acoustic responsiveness of MBs, producing unpredictable outcomes. The aim of this study is to reveal how the surface loaded drug (doxorubicin, DOX) affects the acoustic properties of MBs. A suitable formulation of MBs for DOX loading was first identified by regulating the proportion of two lipid materials (1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 1,2-distearoyl-sn-glycero-3-phospho-rac-glycerol sodium salt (DSPG)) with distinct electrostatic properties. We found that the DOX loading capacity of MBs was determined by the proportion of DSPG, since there was an electrostatic interaction with DOX. The DOX payload reduced the lipid fluidity of MBs, although this effect was dependent on the spatial uniformity of DOX on the MB shell surface. Loading DOX onto MBs enhanced acoustic stability 1.5-fold, decreased the resonance frequency from 12–14 MHz to 5–7 MHz, and reduced stable cavitation dose by 1.5-fold, but did not affect the stable cavitation threshold (300 kPa). Our study demonstrated that the DOX reduces lipid fluidity and decreases the elasticity of the MB shell, thereby influencing the acoustic properties of MBs.

Measurement and Characterisation of a Diffuse Acoustic Field Using a Phased Array

A diffuse acoustic field has been increasingly used to infer temporal changes in the structures, such as early dislocations and microcracking. This study explores three different techniques to characterise acoustic field by using a single ultrasonic phased array. The first two techniques are proposed to measure spatial uniformity of wave field by examining differences in the integral of energy and the maximum energy respectively at multiple inspection locations. The third one is developed to evaluate the degree of phase coherence between propagating waves transmitted sequentially by two neighbouring array elements. The efficacy of these techniques are investigated by examining their metrics on simulations and well-known samples. The results suggest that two selected metrics can be used to quantitatively estimate the diffuse field start time as well as the field size by comparing their value with the idealised diffuse state (15% for the energy integral metric, ηarea and 1 for the phase coherence metric, ηphase) and identifying the convergence start point.

Experimental Study on the Compaction Characteristics and Evaluation Method of Coarse-Grained Materials for Subgrade

Coarse-grained materials are widely used in high-speed railway construction, and it is of great significance to research its compaction characteristics due to the high quality control requirements. In this regard, a field compaction experiment was conducted at a subgrade near Bazhou Station of Beijing-Xiong’an Intercity Railway. The test results of the compaction effect were presented in this study at first. The roller-integrated compaction measurements (i.e., compaction meter value, CMV) were compared with several traditional in-situ tests (i.e., plate load test, light falling weight deflectometer test, and shear wave velocity test). Then the stability of CMV was evaluated by the proposed δ criterion. The spatial uniformity of compaction was further investigated. Based on the analysis, the target value of CMV was preliminarily determined. It showed that Evd was more variable than CMV. The results convincingly indicated that the compaction parameters increased with the increasing number of roller passes at first. A further increase in compaction effort could result in the decompaction of material when the compaction number up to a certain value. The stability analysis method proposed in this study showed its potency of quantifying the percentage of areas with acceptable compaction. The geostatistical analysis could reflect the spatial uniformity of compaction. Overall, the conducted study could provide a useful reference for geo-material compaction control in the transportation engineering.

Screening current induced field characteristics of HTS quasi-isotropic and simply stacked strands in alternating magnetic field

The screening current induced field (SCIF) in the flat REBCO coated conductors (REBCO CCs) so called 2G HTS tapes cause undesirable effects in multiple applications. Their existence reduces the spatial uniformity and temporal stability of magnetic fields for applications of superconducting magnets. In this paper, we numerically and experimentally investigate the characterization of the screening current and SCIF of quasi-isotropic strand (Q-IS) and simply stacked strand (SSS) under external alternating magnetic field with various amplitudes, orientations, and excitation rates. The two-dimensional finite element method (2D FEM) based on T-A formulation is adopted for simulation, the Q-IS and SSS samples are fabricated for experiments. The field angle is in the range of 0° to 90° at intervals of 15°, the excitation rate varies from 20 mT/s to 800 mT/s. We display the distribution of screening current in both strands under various field amplitudes and orientations. Then the dependence of SCIF on the amplitude and orientation of external field is studied, respectively. The spatial distribution of SCIF of both strands with different amplitudes and angles of the external field are also discussed. Besides, we analyze the properties of SCIF under various excitation rates. As a result, the SCIF of Q-IS is much smaller and has quasi-isotropic distribution comparing with SSS, which represents that Q-IS has relative smaller screening effect. The spatial point with the largest SCIF of Q-IS locates at the corner of the strand and is independent of the external field, but the corresponding point in SSS varies with the angle and amplitude. The Q-IS is also less susceptible to the change of rate. Therefore, Q-IS has more advantages when the screening effect is considered in superconducting applications.

Shape Matters: Plant Architecture Affects Chemical Uniformity in Large-Size Medical Cannabis Plants

Since plant organs sense their environment locally, gradients of micro-climates in the plant shoot may induce spatial variability in the physiological state of the plant tissue and hence secondary metabolism. Therefore, plant architecture, which affects micro-climate in the shoot, may considerably affect the uniformity of cannabinoids in the Cannabis sativa plant, which has significant pharmaceutical and economic importance. Variability of micro-climates in plant shoots intensifies with the increase in plant size, largely due to an increase in inter-shoot shading. In this study, we therefore focused on the interplay between shoot architecture and the cannabinoid profile in large cannabis plants, ~2.5 m in height, with the goal to harness architecture modulation for the standardization of cannabinoid concentrations in large plants. We hypothesized that (i) a gradient of light intensity along the plants is accompanied by changes to the cannabinoid profile, and (ii) manipulations of plant architecture that increase light penetration to the plant increase cannabinoid uniformity and yield biomass. To test these hypotheses, we investigated effects of eight plant architecture manipulation treatments involving branch removals, defoliation, and pruning on plant morpho-physiology, inflorescence yield, cannabinoid profile, and uniformity. The results revealed that low cannabinoid concentrations in inflorescences at the bottom of the plants correlate with low light penetration, and that increasing light penetration by defoliation or removal of bottom branches and leaves increases cannabinoid concentrations locally and thereby through spatial uniformity, thus supporting the hypotheses. Taken together, the results reveal that shoot architectural modulation can be utilized to increase cannabinoid standardization in large cannabis plants, and that the cannabinoid profile in an inflorescence is an outcome of exogenous and endogenous factors.

Spatial uniformity tolerances for sound masking systems in open-plan offices

Electronic sound masking systems raise the ambient sound level in offices to a controlled minimum sound level in order to increase speech privacy and reduce distractions. Sound masking systems are calibrated to provide the most uniform sound field achievable, as a spatially non-uniform masking sound field could result in occupant perception and uneven speech privacy conditions. Tolerances for acceptable spatial uniformity vary between specifiers, and may be based on different evaluation methods using only a few discrete measurement points to represent an entire office space. However, the actual uniformity of a masking sound field across an office, and the parameters influencing it, has not been widely investigated. Thus, this study aims to investigate the masking sound uniformity in a typical open-plan office space using fine-grid measurements conforming to measurement method of ASTM E1573-18. Percentages of measured locations where the sound pressure levels were within specified tolerances (with increments of 0.5 dB) were calculated using the measured 1/3 octave band levels. The research also utilized geometric acoustical simulations to investigate how physical office parameters (number of loudspeakers, partition heights, ceiling absorption, and diffusion characteristics) affect the sound field uniformity of the sound masking system.

Global Land Cover Assessment Using Spatial Uniformity Validation Dataset

The Degree Confluence Project (DCP) is a volunteer-based validation dataset that comprises useful information for global land cover map validation. However, there is a problem with using DCP points as validation data for the accuracy assessment of land cover maps. While resolutions of typical global land cover maps are several hundred meters to several kilometers, DCP points can only guarantee an area of several tens of meters that can be confirmed by ground photographs. So, the objective of this study is to create a land cover map validation dataset with added spatial uniformity information using satellite images and DCP points. For this, we devised a new method to semiautomatically guarantee the spatial uniformity of DCP validation data points at any resolution. This method can judge the validation data with guaranteed uniformity with a user’s accuracy of 0.954. Furthermore, we conducted the accuracy assessment for the existing global land cover maps by the DCP validation data with guaranteed spatial uniformity and found that the trends differed by class and region.

Enhancing and quantifying spatial homogeneity in monolayer WS2

Controlling the radiative properties of monolayer transition metal dichalcogenides is key to the development of atomically thin optoelectronic devices applicable to a wide range of industries. A common problem for exfoliated materials is the inherent disorder causing spatially varying nonradiative losses and therefore inhomogeneity. Here we demonstrate a five-fold reduction in the spatial inhomogeneity in monolayer WS2, resulting in enhanced overall photoluminescence emission and quality of WS2 flakes, by using an ambient-compatible laser illumination process. We propose a method to quantify spatial uniformity using statistics of spectral photoluminescence mapping. Analysis of the dynamic spectral changes shows that the enhancement is due to a spatially sensitive reduction of the charged exciton spectral weighting. The methods presented here are based on widely adopted instrumentation. They can be easily automated, making them ideal candidates for quality assessment of transition metal dichalcogenide materials, both in the laboratory and industrial environments.

Fabrication and study of laboratory scale rainfall simulator for soil erosion assessment

A rainfall simulator is an ideal tool for infiltration, soil erosion and other related research areas for replicating the process and characteristics of natural rainfall. The present paper describes the design of a comprehensive rainfall simulator. In this study a laboratory scale rainfall simulator is developed, which is particularly meant for the assessment of soil erosion at plot scale by considering various soil grain types, soil slope angles and surface exposures under different rainfall conditions. The Rainfall characteristics including the rainfall intensity and its spatial uniformity raindrop size and kinetic energy confirm that natural rainfall conditions are simulated with sufficient accuracy. The comparative measurement was carried out in a laboratory using rainfall simulator fabricated of 4 feet length and 2.5 feet width, where the applied slope angle is 3% with 39 mm/hr rainfall intensity. The runoff and soil loss for different samples were assessed by conducting number of trials. From the results it was found that the soil tilled and keeping it as a bare plot is more prone to runoff compared to soil without tilled and straw mulching has helped to reduce the runoff by 57% as compared to soil without mulching.