In vitro behavior of Mycoplasmagallisepticum live-type nosode

As a step of a doctoral research project, in this study a live-type nosode was prepared from microorganism Mycoplasmagallisepticum strain R (ATCC 93-08/19610) according to Costa model and the rules by Brazilian Homeopathic Pharmacopoeia. Live nosode was tested in vitro to assess safety when used to immunize domestic fowl (Gallus gallus) against infection by this microorganism and to investigate its behavior under laboratory conditions. M. gallisepticum was not shown to grow in fluid (broth) and solid (plate) modified Frey medium with dilutions 11d, 12d, 20d and 30d. Inhibition halos about 2.0 mm were observed around paper disks impregnated with live-type nosode in microorganism-sown Petri dishes, whereas disks impregnated with conventional antibiotic oxytetracycline exhibited 8.0 mm inhibition halos. Protein assessment by Folin-Lowry method showed protein absence in dilutions 12d and 30d and neither microbial DNA traces were found in PCR assay in dilutions 12d, 20d and 30d.

Aerodynamic Characteristics of Radar Antenna in Stationary and Azimuthal Rotational Motion

To study the effects of aerodynamic loads on the aerodynamic characteristics of stationary and azimuthally rotating antennas, wind tunnel force tests are conducted using solid and porous plate antennas. The variation of aerodynamic coefficient with azimuth angle is obtained when the antenna is stationary and azimuthal rotation, and the results are compared with those from numerical simulations. The variation in the aerodynamic coefficients with respect to the azimuth angle is found to be sinusoidal for both the solid and porous plate antennas rotating in azimuth. Compared with the antenna stationary, quantitative analysis indicates that the rotational motion increases the maximum value and root mean square of the aerodynamic coefficient. For solid plate antenna, |Cx|_max, |Cmy|_max, and |Cmz|_max increase by 41.6%, 15.0%, and 47.3%, respectively; Cx_rms, Cmy_rms, and Cmz_rms increase by 19.0%, 20.0%, and 19.1%, respectively. For porous plate antenna, |Cx|_max, |Cmy|_max, and |Cmz|_max increase by 30.6%, 71.4%, and 40.9%, respectively; Cx_rms, Cmy_rms, and Cmz_rms increase by 22.9%, 50%, and 20%, respectively. The wind tunnel tests verify the feasibility of using numerical simulations to obtain the flow field results. By analyzing the surface pressure coefficient and vortex core track distribution, the effects of azimuthal rotation on the aerodynamic characteristics of the antenna are further clarified.

Biosynthesis of Silver Nanoparticles by Conyza canadensis and Their Antifungal Activity against Bipolaris maydis

Silver nanoparticles were biosynthesized from Conyzacanadensis leaf extract with the help of a microwave oven. The UV-vis spectrum showed the maximum absorption at 441 nm, corresponding to the surface plasmon resonance of silver nanoparticles. Transmission electron microscope and scanning electron microscope images showed that the synthesized silver nanoparticles were spherical or near-spherical with an average diameter of 43.9 nm. X-ray diffraction demonstrated nanoparticles with a single-phase cubic structure. As-synthesized silver nanoparticles displayed prominent antifungal activity against Bipolaris maydis. The colony inhibition rate reached 88.6% when the concentration of nanosilver colloid was 100 μL·mL−1 (v/v). At such a concentration, no colony formation was observed on the solid plate. The diameter of the inhibition zone was 13.20 ± 1.12 mm. These results lay the foundation for the comprehensive control of plant pathogens using an environmentally friendly approach.

Experimental Study of the Effect of Cavities on the Load Capacity of Two-Way Reinforced Concrete Plates on Fixed Concrete Thickness

The study aims to determine the effect of cavities on the load capacity of reinforced concrete slabs when compared to massive reinforced concrete slabs that have the same thickness, with the hope of reducing the structure’s weight and the use of concrete materials. The modified PVC pipes, as cavity formers, will be placed in the tensile area without reducing the flexural strength that is caused by the weak nature of concrete against tensile strength. The test is carried out on a full scale against 14 cm thick solid plates (PP-1), and hollow plates, which use modified PVC pipes (PB-2), with a cavity diameter of 7.6 cm that has the same thickness. The test uses joint supports on all four sides and the loading pattern is evenly distributed. All slabs are made, on the spot, of cast concrete with the same size and distance between the reinforcement. PVC hollow plate (PB-2) has the same effective thickness as solid plate but has 14% less concrete volume. The maximum load capacity on the solid plate (PP-1) is 522.66 kN and on the hollow plate (PB-2) is 444.33 kN. The melting capacity on the solid plate (PP-1) is 373,515 kN and on the hollow plate (PB-2) is 325,935 kN. Initial crack load capacity on the solid plate (PP-1) is 19.5 kN and on the hollow plate (PB-2) is 16.75 kN

Conjugate heat transfer from a microchannel embedded impingement cum film cooled-flat plate

A computation fluid dynamics analysis is presented to investigate the effect of placing a microchannel inside a flat plate. A microchannel embedded flat plate with 250 angled 175 film holes in staggered form is considered in the present work. A Conjugate heat transfer analysis is done to determine the efficiency of cooling. Simulations were carried out, and subsequently, a parametric study was conducted to observe the effect of variation of blowing ratios. The temperature distribution is observed to be more uniform due to the presence of the microchannel, resulting in a lesser thermal gradient in the solid plate. It is also noted that overall effectiveness increases with the blowing ratio. The maximum increase in overall effectiveness due to the microchannel is about 30% for the blowing ratio of unity.

Enhancing the Methanol Tolerance of Candida antarctica Lipase B by Saturation Mutagenesis for Biodiesel Preparation

Methanol tolerance of a lipase is one of the important factors affecting its esterification ability in biodiesel preparation. By B factor indicated prediction of Candida antarctica lipase B (CalB) surface amino acids, 8 sites (Val139, Ala146, Leu147, Pro218, Val286, Ala287, Val306, and Gly307) with high B value indicating more flexibility were chosen to perform saturation mutagenesis. High-methanol-tolerant variants, CalB-P218W and -V306N, created larger haloes on emulsified tributyrin solid plate including 15% (v/v) methanol and showed 19% and 31% higher activity over CalB-WT (wild type), respectively. By modeling, a newly formed hydrogen bond in CalB-V306N and hydrophobic force in CalB-P218W contributing more stability in protein may have resulted in increased methanol tolerance. CalB-P218W and -V306N transesterified the soybean oil into biodiesel at 30 °C by 85% and 89% yield, respectively, over 82% by CalB-WT for 24 h reactions. These results may provide a basis for molecular engineering of CalB and expand its applications in fuel industries. The as-developed semi-rational method could be utilized to enhance the stabilities of many other industrial enzymes.

Behavior Hollow Concrete Reinforced Slab with The Utilization of Polyvivyl Chloride Pipe as a Cavity

Background: Slab weight can be reduced by replacing the amount of concrete in the tensile area with the utilization of modification polyvinyl chloride pipe as cavity without reducing the flexural strength because the nature of concrete is weak against tensile strength. Methods: This research with the experimental method using static load. The setting of tools refers to the American Society for Testing and Material E 2322, bending analysis is used moment coefficient method, deflection by applying Ugural and Navier method, and shear analysis using Indonesia National Standard 03-2847-2019. Results: The specimens consist of solid plates with a thickness of 140 mm and hollow plates with a thickness of 140 mm and 159 mm. The maximum load capacity solid plate is 410.642 kN, while the hollow plates (140 mm) is 335.18 kN, and for the hollow plates 159 mm is 396.257 kN. The solid plate’s stiffness is 16.321, the hollow plates 140 mm is 14.787 and the hollow plate 159 mm is 24.194, while the ductility is 1.993 on solid plate, the hollow plate 140 mm is 2.014, and the hollow plate 159 mm is 1.862. The solid plate’s damage is flexural, while the two hollow plates are a combination of bending and shear damage. The crack pattern on the solid plates is flexible, while the crack pattern on both hollow plates is a combination of flexural cracks and shear cracks. Conclusion: The use of modified polyvinyl chloride pipes can be recommended in the structure of the plate. There is a small, insignificant difference in the flexural behaviour between the solid plate and hollow concrete reinforced slab with the utilization of Polyvinyl Chloride.

Arched Decorations from Burial Grounds in the Vetluga-Vyatka Interfluve of 10th – 11th Centuries

The article deals with arched decorations from the burial grounds in the Vetluga–Vyatka interfluve of the 9th – 10th centuries. Some of the decorations are introduced into scientific discourse for the first time. Two groups of items are revealed: 1) pendants with a solid plate, 2) pendants with a "slotted" base. The pendants of the first group are original in their manufacturing method, ornamentation and additional details. The arched shields of pendants of the second group with the image of a sprout, three-sun and a rhombus are similar to products of the Perm Cis-Urals, South Urals, Bolshiye Tigany and Tankeyevka burial grounds, which have a connection with the Ugric culture. Despite the similarity of the main pendant shields, these decorations in the Vetluga-Vyatka interfluve have a special set of component parts, ways of connecting the base with additional pendants. Both groups are characterized by pendants in the form of 2 connected spirals, which make the decoration original. So, the arched pendants of both groups are not an exact copy of the adornments of neighboring territories; they have their own appearance and their original use in costume as breast adornments. It is quite possible that the shields for adornments of the second group were brought from the Cis-Urals, but the decoration was completely formed on the spot in accordance with their ideas and traditions.