Abstract
Among protein-containing biomass waste, waste animal wool, poultry feather, and human hair are considered as one of the most important renewable sources of keratin. Although waste animal wool (WAW) and human hair (WHH) are used for the production of number of products but the substantial quantity of short fibers not suitable for spinning and being unusable is thrown as waste resulting considerable environmental problem in terms of their accumulation in water bodies resulting water clogging and other related problems. Similarly, poultry waste especially the waste chicken feathers (WCF) is dumped or burnt, or used as low value fertilizer in certain applications. To extract more value-added products such as keratin from such wastes, herein suitability of an aqueous solution of quaternary ammonium hydroxide known as tetramethylammonium hydroxide (25% w/w TMAOH in water) to solubilize these protein wastes and to extract keratin from them was investigated. The solvent could solubilized ca. 39–44% w/w of WAW, 19–25% of WHH, and 55–60% of WCF. Crude keratin with ca 19–20%, 35–37%, and 69–74% were isolated from WAW, WHH, and WCF respectively. The chemical and structural stability of keratin thus isolated was established. The recovered TMAOH, insoluble WAW and WCF were found to be nontoxic to soil microbes. The recovered TMAOH thus generated after isolation of keratin was used for green gram (Vigna radiata) seed treatment and a substantial increase in the height (4–12%) and weight (9–58%) of the plants were observed. Treating biomass waste as a source of high value compounds may minimize environmental impact by reducing the waste load.