Laser-Irradiated Chlorpromazine as a Potent Anti-Biofilm Agent for Coating of Biomedical Devices

Nowadays, antibiotic resistance has become increasingly common, triggering a global health crisis, immediately needing alternative, including repurposed drugs with potent bactericidal effects. We demonstrated that chlorpromazine aqueous solutions exposed to laser radiation exhibited visible activity against various microorganisms. The aim of this study was to investigate the quantitative antimicrobial activity of chlorpromazine in non-irradiated and 4-h laser irradiated form. Also, we examined the effect of both solutions impregnated on a cotton patch, cannula, and urinary catheter against Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa and Escherichia coli. In all experimental versions, the chlorpromazine antimicrobial activity was enhanced by laser exposure. Besides the experimental results, the in silico analyses using molecular docking proved that the improved antimicrobial activity of the irradiated compound was a result of the combined action of the photoproducts on the biological target (s). Our results show that laser radiation could alter the molecular structure of various drugs and their effects, proving to be a promising strategy to halt antibiotic resistance, by repurposing current medicines for new antimicrobial strategies, thereby decreasing the costs and time for the development of more efficient drugs.

P203 FIT and FC as a surrogate non-invasive marker for mucosal healing in inflammatory bowel disease

Background Mucosal healing (MH) is one of the goals of IBD therapy. Currently, the only way to assess MH is by colonoscopy. Faecal calprotectin (FC) and faecal immunochemical test (FIT) which detect colonic inflammation and bleeding might be useful adjuncts. Aim To assess FIT and FC as surrogate markers of MH. Methods Following ethical approval, patients undergoing routine colonoscopy were prospectively recruited. Demographics and colonoscopy findings were documented. A FIT and FC were collected prior to colonoscopy. FIT and FC were processed in our laboratory and reported as µg/g of stool. MH was defined no visible activity on colonoscopy. Results Of 105 colonoscopies, FC and FIT results were available in 99 and 88 patients. Mean age 48.8, 52% (55) males, 34% (36) UC, 60% (63) Crohn’s, 0.05% (6) IBDU. In all, MH occurred in 12%, (n = 12). In MH, the mean FIT and FC were 1142 and 353, while in active cases were 750 and 819. Only FC was significantly lower in MH cases (353 vs. 819, p = 0.05). Using standard cut-offs of >50; sensitivity, specificity, PPV and NPV for MH for FIT was poor; 59%, 33%, 89%, 9% and for FC was better at 74%, 73%, 91%, 44%. The overall correlation between the biomarkers was weak r = 0.2, p < 0.01. FC ROC analysis gave a specificity and sensitivity of 75% and 67% for a cut-off of <64µg/g, AUC = 0.65. Conclusion MH was uncommon (12%) reflecting our clinical practice. FIT was a poor predictor of MH. FC might be a useful marker albeit ongoing research is needed to set an appropriate cut-off.

Antimicrobial Activity of Bee Bread Extracts Against Different Bacterial Strains

Bee bread is a product of the hive obtained from pollen collected by bees, to which they add honey, digestive enzymes and subsequently is stored in the combs. Increasing evidence suggests bee bread’s potential therapeutic benefits, including antimicrobial properties. Bee bread is characterized by a bactericidal and bacteriostatic activity. The current study was carried out to test the antimicrobial activity of bee bread extracts, in various concentrations, against the bacterial strains: Bacillus cereus, Staphylococcus aureus, Escherichia coli, Salmonella enterica and Pseudomonas aeruginosa. The results of this study indicate that the first two dilutions of bee bread extract, respectively 33% and 16.66%, showed higher antimicrobial activity and the other dilutions had a lower, but visible activity depending on the pathogen on which they are tested. The best antimicrobial activity was manifested on the Staphylococcus aureus strain, where all dilutions had an inhibitory effect both at 8 hours and 12 hours.

MODEL OF EMOTIONAL INTELLIGENCE AND SOCIAL COMPETENCE DEVELOPMENT IN CHILDREN OF SENIOR PRESCHOOL AGE IN COLLECTIVE VISIBLE ACTIVITY

The problem of the development of emotional intelligence and social competence in childhood is a new direction of scientific research in pedagogical psychology and pedagogy, which is at the stage of formation. The lack of unity of views on the problem of emotional intelligence in the scientific community generates many interpretations of this phenomenon. Relying on the concept of social competence of the famous Russian scientist A.I. Savenkov, who defines emotional intelligence as an integral part (substructure) of social competence, we substantiate the potential of various forms of organization of joint activities of children and propose a model for the development of emotional intelligence and social competence of older preschool children in collective visual activity. Interaction of children in different forms of joint visual activity provides teachers and psychologists with a rich base for empirical observation of behavioral manifestations of emotional intelligence, the evaluation of which is very important for a deeper understanding of this phenomenon. The implementation of the model will allow to update all the basic parameters of emotional intelligence and social competence (cognitive, emotional, behavioral), to contribute to the development of the content of procedures for identifying and quantifying each of the basic parameters, as well as the methods for their diagnosis and development in the course of activities of different subject-matter (artistic, research and etc.).