KEEP THE PACE: THE UNCORKED POTENTIAL OF WINE TOURISM IN ŠUMADIJA

In the last two decades, Serbia has begun to re-establish itself within other wine-producing countries. One of the Serbian wine regions – Šumadija – caught hold of this opportunity and launched in the wine tourism industry. This paper, based on visits to Šumadija wineries and interviews with winemakers, is a survey of the present tourism infrastructures and wine histories. The aim is to explore the capacity of the wine industry in its attempt to develop tourism as a component of regional developmental progress. It is a review of the wine tourism situation and development in the Šumadija region, a survey of where it is today, and an assessment of its potential in the future. The author conducted interviews with winemakers and collected primary data from tourists at the regional wine event in 2017. In conclusion, recommendations are made for the further development of the area as a wine destination.

Transcriptomics Provides a Genetic Signature of Vineyard Site and Offers Insight into Vintage-Independent Inoculated Fermentation Outcomes

ABSTRACT Ribosomal DNA amplicon sequencing of grape musts has demonstrated that microorganisms occur nonrandomly and are associated with the vineyard of origin, suggesting a role for the vineyard, grape, and wine microbiome in shaping wine fermentation outcomes. Here, ribosomal DNA amplicon sequencing from grape musts and RNA sequencing of eukaryotic transcripts from primary fermentations inoculated with the wine yeast Saccharomyces cerevisiae RC212 were used to profile fermentations from 15 vineyards in California and Oregon across two vintages. These data demonstrate that the relative abundance of fungal organisms detected by ribosomal DNA amplicon sequencing correlated with neither transcript abundance from those same organisms within the RNA sequencing data nor gene expression of the inoculated RC212 yeast strain. These data suggest that the majority of the fungi detected in must by ribosomal DNA amplicon sequencing were not active during the primary stage of these inoculated fermentations and were not a major factor in determining RC212 gene expression. However, unique genetic signatures were detected within the ribosomal DNA amplicon and eukaryotic transcriptomic sequencing that were predictive of vineyard site and region. These signatures included S. cerevisiae gene expression patterns linked to nitrogen, sulfur, and thiamine metabolism. These genetic signatures of site offer insight into specific environmental factors to consider with respect to fermentation outcomes and vineyard site and regional wine characteristics. IMPORTANCE The wine industry generates billions of dollars of revenue annually, and economic productivity is in part associated with regional distinctiveness of wine sensory attributes. Microorganisms associated with grapes and wineries are influenced by region of origin, and given that some microorganisms play a role in fermentation, it is thought that microbes may contribute to the regional distinctiveness of wine. In this work, as in previous studies, it is demonstrated that specific bacteria and fungi are associated with individual wine regions and vineyard sites. However, this work further shows that their presence is not associated with detectable fungal gene expression during the primary fermentation or the expression of specific genes by the inoculate Saccharomyces cerevisiae strain RC212. The detected RC212 gene expression signatures associated with region and vineyard site also allowed the identification of flavor-associated metabolic processes and environmental factors that could impact primary fermentation outcomes. These data offer novel insights into the complexities and subtleties of vineyard-specific inoculated wine fermentation and starting points for future investigations into factors that contribute to regional wine distinctiveness.

Sources and Assembly of Microbial Communities in Vineyards as a Functional Component of Winegrowing

Microbiomes are integral to viticulture and winemaking – collectively termed winegrowing – where diverse fungi and bacteria can exert positive and negative effects on grape health and wine quality. Wine is a fermented natural product, and the vineyard serves as a key point of entry for quality-modulating microbiota, particularly in wine fermentations that are conducted without the addition of exogenous yeasts. Thus, the sources and persistence of wine-relevant microbiota in vineyards critically impact its quality. Site-specific variations in microbiota within and between vineyards may contribute to regional wine characteristics. This includes distinctions in microbiomes and microbiota at the strain level, which can contribute to wine flavor and aroma, supporting the role of microbes in the accepted notion of terroir as a biological phenomenon. Little is known about the factors driving microbial biodiversity within and between vineyards, or those that influence annual assembly of the fruit microbiome. Fruit is a seasonally ephemeral, yet annually recurrent product of vineyards, and as such, understanding the sources of microbiota in vineyards is critical to the assessment of whether or not microbial terroir persists with inter-annual stability, and is a key factor in regional wine character, as stable as the geographic distances between vineyards. This review examines the potential sources and vectors of microbiota within vineyards, general rules governing plant microbiome assembly, and how these factors combine to influence plant-microbe interactions relevant to winemaking.

Transcriptomics provides a genetic signature of vineyard site with insight into vintage-independent regional wine characteristics

In wine fermentations, the metabolic activity of both Saccharomyces cerevisiae and non-Saccharomyces organisms impact wine chemistry. Ribosomal DNA amplicon sequencing of grape musts has demonstrated that microorganisms occur non-randomly and are associated with the vineyard of origin, suggesting a role for the vineyard, grape, and wine microbiome in shaping wine fermentation outcomes. We used ribosomal DNA amplicon sequencing of grape must and RNA sequencing of primary fermentations to profile fermentations from 15 vineyards in California and Oregon across two vintages. We find that the relative abundance of fungal organisms detected by ribosomal DNA amplicon sequencing did not correlate with transcript abundance from those organisms within the RNA sequencing data, suggesting that the majority of the fungi detected in must by ribosomal DNA amplicon sequencing are not active during these inoculated fermentations. Additionally, we detect genetic signatures of vineyard site and region during fermentation that are predictive for each vineyard site, identifying nitrogen, sulfur, and thiamine metabolism as important factors for distinguishing vineyard site and region.

Evaluating uncertainty in sensory analysis. A case study of the panel of tasters of the Dão Regional Wine Commission

Metrological reliability in the sensory evaluation of products is of fundamental importance in ensuring the comparability of results. The issue of uncertainties regarding measured data in such contexts has led to new scientific approaches in the field of sensory analysis, which have become fundamental to studies that seek to compare results of sensory measurement and analysis, including studies regarding wine. The new normative framework, ISO/IEC 17025:2017, which replaces the previous version ISO/IEC 17025, published in 2005, draws attention to the concept of measurement uncertainty, stating that it should be considered by laboratories when assessing conformity and quantifying risks arising in decision-making processes. The present work examines the evaluation of uncertainty and its importance for the interpretation of results from sensory analyses, examining sensory analysis tests related to the certification of the Designation of Origin “Dão”, the Designation of Origin “Lafões” and the Geographical Indication “Terras do Dão”, carried out by a panel composed of 20 tasters of the Dão Regional Wine Commission, trained and accredited by the standard NP ISO/IEC 17025:2018. The results achieved led to a conclusion that the panel presented different values of uncertainty regarding the tests to determine sensory attributes. The results and respective values of uncertainty made it possible to assess the performance of the panel of tasters and to establish a level of risk (false accept, false reject and statistical assumptions) for decision rules used when issuing statements of conformity. In this study, a new methodology was presented to determine an estimate of uncertainty to be applied in the interpretation of results related to sensory analysis tests of wines for the certification of Designation of Controlled Origin “Dão”, Designation of Origin “Lafões” and Geographical Indication “Terras do Dão. This progress led to an evolution in the day-to-day work of the Dão Regional Wine Commission tasting chamber enabling greater solidity in the opinions issued, in line with the requirements of the reference standard NP ISO/IEC 17025: 2018.

Le lexique viticole regional dans l’Ouest de la France : une analyse socio-historique sur corpus

"Regional Wine Terminology in Western France: a Sociohistorical Corpora-based Terminology. In a highly standardised language like French, wine terminology seems largely influenced by national and supranational standards, marked by specialists and diffused through professional training or specialised publications, for instance. Yet, in general, terms referring to wine are at the same time rooted in a territory. Where do they come from, and how do they pass from one area or group of speakers to another? How do people perceive them? Are they necessarily of vernacular tradition? For the study of regional wine terminology, this article focuses on a traditional wine-growing area in France (Poitou-Charentes). It combines the methods of historical linguistics in order to trace the origin and diffusion of regionalisms retrieved from a contemporary corpus with a sociolinguistic analysis of their status through discourse analysis and enquiries. The article analyses more than twenty dialectal terms, revealing their distribution in time and space as well as their legitimacy in current usage. Keywords: wine terminology, Western France, French historical lexicology, dialectology, sociolinguistic enquiries, discourse analysis."

Firm Performance and Exports: Evidence from the Romanian Wine Industry

This paper examines the impact of exports and its main determinants on the financial performance of the Romanian wine industry. We draw on a dataset consisting of mixed firm-level (i.e., 207 companies) data, Google Trends data, and regional variables covering the period from 2009 to 2017. We show that Romanian wine exports, at the firm level, are positively affected by regional wine yields (especially in the case of red wine varieties), temperature, and firm agglomeration, and negatively impacted by firm size. We also find a close positive correlation between financial performance and exports. (JEL Classifications: F61, L66, C23)

Exploring the Influence of Regional Brand Equity in an Emerging Wine Sector

We measure the effects of consumer-perceived regional brand equity on future intentions toward regional wine purchases and winery visits for U.S.-based North Carolina (NC) wines. Visitor data were collected at 23 regional wineries, including demographics, motives, and perceptions of and intentions toward NC wines. SPSS™ and SmartPLS™ were employed in analyzing the model presented. Knowledge and regional wine brand equity influence wine consumer motives, which in turn affect intentions. Product-centered motives were more important than experience motives for visiting regional wineries. (JEL Classifications: L66, L83, M31)