Ketone Body Rescued Seizure Behavior Of LRP1 Deficiency By Modulating Glutamate Transport

LRP1, the low-density lipoprotein receptor 1, would be a novel candidate epilepsy gene according to our bioinformatic results and the animal study. In this study, we explored the role of LRP1 in Epilepsy and whether Beta-hydroxybutyrate, the principal ketone body of the ketogenic diet can treat epilepsy caused by LRP1 deficiency. UAS/GAL4 system was used to establish different genotype models. Flies were given Standard, High-sucrose, and ketone body food randomly. The bang-sensitive test was performed on flies and seizure-like behavior was assessed. Morphologic alteration of LRP1 defect in the brain was detected under GPF expression flies. We established global, astrocytic, and neuronal LRP1 knockdown flies. Whole body and glia LRP1 defect flies had a higher seizure rate compared to the control group in the behavior test. Ketone body decreased the seizure rate in behavior test in all LRP1 defect flies, compared to Standard and High sucrose diet. In morphologic experiments, we found that LRP1 deficiency caused partial loss of the ellipsoidal body and partial destruction of the fan-shaped body. Overexpression of glutamate transporter gene Eaat1 could mimic the ketone body effect on LRP1 deficiency flies. This study demonstrated that LRP1 defect globally or in astrocytes or neurons could induce epilepsy. The ketone body efficaciously rescued epilepsy caused by LRP1 knockdown. The results support screening for LRP1 mutations as discriminating conduct for individuals who require clinical attention and further clarify the mechanism of the ketogenic diet in Epilepsy, which could help Epilepsy patients making a precise treatment case by case.

Possible role of intravenous administration of mesenchymal stem cells to alleviate interstitial cystitis/bladder pain syndrome in a Toll-like receptor-7 agonist-induced experimental animal model in rat

Background Interstitial cystitis/bladder pain syndrome (IC/BPS) categorized with and without Hunner lesions is a condition that displays chronic pelvic pain related to the bladder with no efficacious treatment options. There are strong associations suggested between Hunner-type IC and autoimmune diseases. Recently, we established an animal model of Hunner-type IC using a Toll-like receptor-7 (TLR7) agonist. Intravenous infusion of mesenchymal stem cells (MSCs) can be used to treat injury via multimodal and orchestrated therapeutic mechanisms including anti-inflammatory effects. Here, we investigated whether infused MSCs elicit therapeutic efficacy associated with the TLR7-related anti-inflammatory pathway in our Hunner-type IC model. Methods Voiding behaviors were monitored 24 h prior to the Loxoribine (LX), which is a TLR7 agonist instillation in order to establish a Hunner-type IC model (from − 24 to 0 h) in female Sprague–Dawley rats. LX was instilled transurethrally into the bladder. At 0 h, the initial freezing behavior test confirmed that no freezing behavior was observed in any of the animals. The LX-instilled animals were randomized. Randomized LX-instilled rats were intravenously infused with MSCs or with vehicle through the right external jugular vein. Sampling tissue for green fluorescent protein (GFP)-positive MSCs were carried out at 48 h. Second voiding behavior tests were monitored from 72 to 96 h. After the final evaluation of the freezing behavior test at 96 h after LX instillation (72 h after MSC or vehicle infusion), histological evaluation with H&E staining and quantitative real-time polymerase chain reaction (RT-PCR) to analyze the mRNA expression levels of inflammatory cytokines were performed. Results Freezing behavior was reduced in the MSC group, and voiding behavior in the MSC group did not deteriorate. Hematoxylin–eosin staining showed that mucosal edema, leukocyte infiltration, and hemorrhage were suppressed in the MSC group. The relative expression of interferon-β mRNA in the bladder of the MSC group was inhibited. Numerous GFP-positive MSCs were distributed mainly in the submucosal and mucosal layers of the inflammatory bladder wall. Conclusion Intravenous infusion of MSCs may have therapeutic efficacy in a LX-instilled Hunner-type IC rat model via a TLR7-related anti-inflammatory pathway.

Effect of short-term high tryptophan diet fed to sows on their subsequent piglet behavior

Housing sows in groups creates the challenge of decreasing fighting amongst sows. One proposed method to do so is to feed a high tryptophan diet, but the effect on the fetus is unkown. To investigate this, 66 sows were fed 1 of 3 diets: Control (0.14 % SID tryptophan), Medium (0.28 % SID tryptophan), or High (0.4 2% SID tryptophan), from d 28 to 35 of gestation. Sows gestated in standard gestation stalls. Blood samples were taken on d 27 prior to and on d 35 after tryptophan supplementation. On d 1 and d 2, 3 nursing bouts were observed so as to record disputes and displacements from teat competition. The piglets’ activity and fighting were recorded on d 3, 7, and 11 from 0700 h to 1700 h. On d 12, 4 piglets per litter were blood sampled, 2 to be used in later behavior tests and 2 to act as controls for blood cortisol levels. On d 14, the 2 behavior test piglets from each litter were subjected to a 10-min Isolation Test and 5-min Human Approach Test. On d 15, the behavior test piglets were paired by sex and treatment (for example, a male Medium piglet paired with another male Medium piglet from a different crate) and each pair was subjected to a 10-min Social Challenge Test and immediately blood sampled. Piglet cortisol and serotonin did not differ among treatments (P > 0.10). There were no differences (P > 0.10) for number born (12.7 ± 0.4), born alive (11.7 ± 0.4), or mortality (1.1 ± 0.2). Behavior during nursing bouts was similar, with no treatment differences in number of disputes or displacements, and similar bout lengths among treatments (199.5 ± 4.6 s, P > 0.10). No differences were detected for any of the variables for Isolation or the Human Approach Tests (P > 0.10). During the Social Challenge Test, High piglets had more contacts approaching the head of the companion piglet than did either Medium or Control piglets (14.3 ± 1.1, 10.7 ± 1.1, and 9.69 ± 0.8 respectively, P < 0.02). Total number of aggressive interactions during the test tended to be greater for Medium piglets compared to High piglets (9.3 ± 1.5 vs 5.1 ± 0.9, P < 0.07). Time budget data of the litter indicate that piglets from all 3 treatments spent equal amounts of time active and inactive (P > 0.10). Aggression was low with 0.3 ± 0.04 % of piglets displaying aggressive behavior. Feeding high concentrations of tryptophan for a short duration early in gestation does not have a negative impact on sows’ subsequent offspring.

Neuroendocrine and Cardiovascular Activation During Aggressive Reactivity in Dogs

Our aim was to investigate cardiovascular activation by measuring changes in facial and body surface temperature using infrared thermography, and neuroendocrine activation using salivary cortisol (CORT) and serotonin concentration (SER) in dogs exhibiting aggressive reactivity in real time. Based on two factors, owner-reported past aggressive behaviors, and detailed behavioral observations collected during a Socially Acceptable Behavior test consisting of 16 subtests and, each individual was categorized as aggressive or non-aggressive. CORT and SER showed no difference in neuroendocrine activity between dogs, but aggressive dogs with higher levels of aggression were found to have lower SER. Aggressive dogs also had an increase in facial temperature from pre-test values. The discovery of a correlation between tail wagging and left tail wagging with aggression level and aggression-related behaviors in aggressive dogs is further evidence of the right hemisphere specialization for aggression previously reported in the literature. This study provides the first evidence that both cardiovascular and neuroendocrine systems are activated during an active act of aggression in dogs.

Sense of purpose in life and motivation, barriers, and engagement in physical activity and sedentary behavior: Test of a mediational model

Purpose in life is associated with greater engagement in physical activity. The present research ( N = 3473) addresses whether motivations and barriers to physical activity mediate this relation concurrently and prospectively early in the coronavirus pandemic. Higher purpose was associated with greater intrinsic, identified, and external motivation for physical activity and fewer barriers. Intrinsic motivation was a robust mediator of the relation between purpose and more physical activity and less sedentary behavior reported concurrently and prospectively; identified motivation mediated the relation with physical activity. Purpose in life is associated with being more physically active through both greater enjoyment and valuing its benefits.

Validation of a Behavior Test for Predicting Puppies’ Suitability as Detection Dogs

Behavioral characteristics are the most influential factor in the success of a working dog. The need for highly capable detection dogs continues to rise; but reliable methods for early selection are lacking. The current study aimed to assess the reliability and validity of a behavioral test for assessing detection dog suitability. A cohort of candidate detection dog puppies (n = 60) were tested at 3; 5; and 11 months of age; as well as at the completion of training at approximately one year. Tests were designed to assess important detection dog behavioral characteristics such as search ability and fearfulness. Inter-rater reliability was high between independent observers. Convergent validity was demonstrated by comparing Principal Component Analysis (PCA) scores from the behavior test to trainer ratings using the Canine Behavioral Assessment and Research Questionnaire (C-BARQ) and a survey of detection dog traits. Performance on the behavior test predicted adult selection as a detection dog as early as 3 months. The methods reported will be valuable for improving selection measures and enhancing collaborations across breeding programs in order to increase the availability of highly capable detection dogs.

PSYCHODIAGNOSTICS AND PSYCHOCORECTION OF BEHAVIORAL DISORDERS AND DISADAPTATION IN ADOLESCENTS WITH PSYCHOLOGICAL DEPRIVATION

Aim of the study on the basis of study of abnormal psychology phenomenology to learn propensity to disorders of behavior and displays of disadaptation for teenagers, as a factor of origin and realization of different forms of victim behavior that can become reason of origin of propensity to the destructive options and tendencies of behavior in interpersonality relations for teenagers with a psychological deprivate and to work out fitting for their psychocorrection.Materials and methods. By us were inspected from 2018 to 2020 50 teenagers by age 16-18, that was brought up shelter of domestic type for the children of orphans and children confined of the paternal caring.Experimental psychological researches included: test of А.Н. Orel is for the exposure of indexes of propensity to disorders of behavior of teenagers, that sent to the estimation of readiness or inclination of teenagers to realization of different forms of deviative behavior; test of О.О. Andronikova for the exposure of indexes of propensity to victim behavior of teenagers.Results. As a result of this study, psychodiagnostics and psychocorrection were carried out to 50 teenagers with a psychological deprivate, it was educed greater propensity to victim behavior and characteristic intentionally creation or provocation of conflict situation for the pupils of refuge for children-orphans, than for teenagers that was brought up in families.Conclusions. On the basis of analysis of individual, personality, social-psychological factors, that stipulate forming of social psychological rejections in behavior of teenagers with psychological deprivation, necessary application of timely complex, psychocorrection, due to renewal for the teenagers of social psychological adaptation, also on psychoprophylaxis of forming of victim and aggressive character traits.