EFECTO INHIBITORIO DE Lactobacillus plantarum AISLADOS DESDE LAS DISTINTAS ETAPAS DE LA CADENA PRODUCTIVA DE CARNE PORCINA FRENTE A Campylobacter coli

Lactobacillus plantarum has a great capacity to adapt to environmental and have antibacterial capacity against different pathogen. This is a potential alternative for the control of pathogen in food coming slaughter animal. The objective of this work was to determine the antibacterial effect of L. plantarum strains against C. coli strains. L. plantarum LP1, LP3, LP5 and LP7 of porcine origin and a reference strain of Campylobacter coli NCTC 11366 were used. C. coli and L. plantarum was reactivated. Lyophilized ELC and ELCn were obtained. C. coli was distributed on the surface of mCCDA by swab and the ELC and the ELCn were inoculated in wells previously made on the agar. After 48 h, the diameter of the zone of inhibition was measured. The inhibition halos produced were 23.2 mm. The ELCn showed less antagonistic activity. The LP5 ELC generated higher inhibition halos and ELCn showed no differences between the four strains studied. L. plantarum strains, isolated from the pig production chain, could potentially be applied to control C. coli. This data is added to the scarce scientific evidence of the inhibitory effect of L. plantarum before this zoonotic pathogen of importance in public health.

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The Inhibitory Effect of Lactobacillus plantarum KY1032 Cell Extract on the Adipogenesis of 3T3-L1 Cells

Journal of Medicinal Food ◽

10.1089/jmf.2010.1355 ◽

2011 ◽

Vol 14 (6) ◽

pp. 670-675 ◽

Cited By ~ 29

Author(s):

Do-Young Park ◽

Young-Tae Ahn ◽

Chul-Sung Huh ◽

Seon-Min Jeon ◽

Myung-Sook Choi

Keyword(s):

Lactobacillus Plantarum ◽

Inhibitory Effect ◽

Cell Extract

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Mortality and Inhibitory Effects of Aqueous and Methanolic Extracts of Artemisia annua L. on Biomphalaria pfeifferi (Krauss) and Shedding of Cercariae

Nigerian Journal of Parasitology ◽

10.4314/njpar.v42i1.4 ◽

2021 ◽

Vol 42 (1) ◽

pp. 25-30

Author(s):

I.M. Ado ◽

Z.A. Ali ◽

M.M. Dogara ◽

K. Abdullahi ◽

S.A Luka ◽

...

Keyword(s):

Methanolic Extract ◽

Artemisia Annua ◽

Scientific Evidence ◽

Standard Procedure ◽

Inhibitory Effect ◽

Control Group ◽

Inhibitory Effects ◽

Biomphalaria Pfeifferi ◽

Artemisia Annua L ◽

Methanolic Extracts

The search for bioactive plants which can be used as non-conventional anthelmintics has received considerable attention in recent times because of the increasing, worldwide development of resistance to synthetic anthelminthes worm populations. However, scientific evidence to validate the use of raw plants materials remain limited. This study evaluated the mortality and inhibitory effects of the crude aqueous and methanolic extract of Artemisia annua L. against the shedding of cercariae of Schistosoma mansoni from Biomphalaria pfeifferi. The phytochemical screening of the plant was done using standard procedure, after which the mortality effects of the plant extracts and effects on the shedding of cercariae from B. pfeifferi snails were assessed for 24 hour of exposure. Methanolic extract with the highest concentration of 1.77mg/µL had an inhibitory effect of 63.06±1.84 while the least concentration with 0.12mg/µL had 22.41±2.17 inhibitory effect. For the aqueous extract, the highest concentration with 2.73mg/µL had an inhibitory effect of 55.75±1.94 while the least concentration of 0.23mg/µL had 21.80±1.45. Inhibitory effect of cercariae in the snail vector was concentration dependent, and there was significance difference (P<0.05) between the treatment mean when compared with the control group. This study has shown that this plant material has some inhibitory effect on the shedding of of S. mansoni cercariae and toxicityeffect on the B. pfeifferisnails, and can therefore be used for the control of the disease causing agent as well as the vector. Keywords: Artemisia annua, Inhibitory effects, cercariae, Biomphalaria pfeifferi

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Comparison of Genotypes and Antibiotic Resistances of Campylobacter jejuni and Campylobacter coli on Chicken Retail Meat and at Slaughter

Applied and Environmental Microbiology ◽

10.1128/aem.00493-13 ◽

2013 ◽

Vol 79 (12) ◽

pp. 3875-3878 ◽

Cited By ~ 13

Author(s):

Sonja Kittl ◽

Bożena M. Korczak ◽

Lilian Niederer ◽

Andreas Baumgartner ◽

Sabina Buettner ◽

...

Keyword(s):

Campylobacter Jejuni ◽

Human Infection ◽

Chicken Meat ◽

Campylobacter Coli ◽

Production Chain ◽

Content Type ◽

Resistance Patterns ◽

Retail Meat ◽

Retail Chicken Meat ◽

Antibiotic Resistance Patterns

ABSTRACTMultilocus sequence typing (MLST) and antibiotic resistance patterns ofCampylobacter jejuniandCampylobacter colifrom retail chicken meat showed high overlap with isolates collected at slaughterhouses, indicating little selection along the production chain. They also showed significant common sequence types with human clinical isolates, revealing chicken meat as a likely source for human infection.

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Potential of Trichosporon asahii against Alternaria sp. and mechanisms of actions

Summa Phytopathologica ◽

10.1590/0100-5405/220861 ◽

2020 ◽

Vol 46 (1) ◽

pp. 20-25

Author(s):

Ariane Silva Bosqueiro ◽

Rodolfo Bizarria Júnior ◽

Márcia Maria Rosa-Magri

Keyword(s):

Antagonistic Activity ◽

Mechanisms Of Action ◽

Antagonistic Effect ◽

Production Chain ◽

Trichosporon Asahii ◽

Modern Agriculture ◽

Killer Toxins ◽

Alternaria Sp ◽

Mycelial Development

ABSTRACT Alternaria sp. is a fungus that causes rot in several crops, causing damage to the agricultural production chain. Biological control agents constitute an alternative to reduce the use of pesticides in the crops, a social demand for modern agriculture. The aim of the present study is to demonstrate the antagonistic effect of the yeast Trichosporon asahii (3S44) on the mycelial development of Alternaria sp. and some in vitro mechanisms of action. Trichosporon asahii showed antagonistic activity towards Alternaria sp., presenting mycelial inhibition (57.47%). The assays indicated production of volatile organic compounds (VOCs), competition for nutrients, biofilm formation, production of killer toxins and β-1,3-glucanase as mechanisms of action of T. asahii. Collectively, our results showed the potential of T. asahii to control the mycelial development of Alternaria sp. and some mechanisms of action of this yeast for future biotechnological studies.

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2,4-EPIBRASSIONOLIDE ACTIVATES PRIMING RESISTANCE AGAINST RHIZOPUS STOLONIFER INFECTION IN PEACH FRUIT

Acta Alimentaria ◽

10.1556/066.2020.49.2.2 ◽

2020 ◽

Vol 49 (2) ◽

pp. 135-143

Author(s):

C.H. Li ◽

M.Y. Du ◽

K.T. Wang

Keyword(s):

Fungal Infection ◽

Inhibitory Effect ◽

The Other ◽

Antifungal Compounds ◽

Rhizopus Stolonifer ◽

Peach Fruit ◽

Direct Inhibitory Effect ◽

Potential Alternative ◽

Direct Toxicity

This study was conducted to assess the effects of 2,4-epibrassionolide (EBR) on mold decay caused by Rhizopus stolonifer and its capability to activate biochemical defense reactions in postharvest peaches. The treatment of EBR at 5 μM possessed the optimum effectiveness on inhibiting the Rhizopus rot in peach fruit among all treatments. The EBR treatment significantly up-regulated the expression levels of a set of defense-related enzymes and PR genes that included PpCHI, PpGns1, PpPAL, PpNPR1, PpPR1 and PpPR4 as well as led to an enhancement for biosynthesis of phenolics and lignins in peaches during the incubation at 20 °C. Interestingly, the EBR-treated peaches exhibited more striking expressions of PR genes and accumulation of antifungal compounds upon inoculation with the pathogen, indicating a priming defense could be activated by EBR. On the other hand, 5 μM EBR exhibited direct toxicity on fungal proliferation of R. stolonifer in vitro. Thus, we concluded that 5 μM EBR inhibited the Rhizopus rot in peach fruit probably by a direct inhibitory effect on pathogen growth and an indirect induction of a priming resistance. These findings provided a potential alternative for control of fungal infection in peaches during the postharvest storage.

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Searching for Antagonistic Activity of Bacterial Isolates Derived from Food Processing Environments on Some Food-Borne Pathogenic Bacteria

Acta Alimentaria ◽

10.1556/066.2020.49.4.7 ◽

2020 ◽

Vol 49 (4) ◽

pp. 415-423

Author(s):

B. Baráti-Deák ◽

Cs. Mohácsi-Farkas ◽

Á. Belák

Keyword(s):

Escherichia Coli ◽

Food Processing ◽

Pathogenic Bacteria ◽

Inhibitory Effect ◽

Antagonistic Activity ◽

Bacterial Isolates ◽

Bacterial Strains ◽

Food Borne ◽

Time Period ◽

Growth Decline

Bacterial strains with inhibitory eﬀect on Salmonella Hartford, Listeria monocytogenes, Yersinia enterocolitica, and Escherichia coli, respectively, were isolated. Out of the 64 bacteria originated from food processing environments, 20 could inhibit at least one of the tested pathogens, and it was proved that growth decline of the pathogenic bacteria was more remarkable by co-culturing than by using cell-free supernatants of the isolates. Seven diﬀerent genera (Pseudomonas, Bacillus, Paenibacillus, Macrococcus, Staphylococcus, Serratia, and Rothia) reduced the pathogens’ growth during the time period of analysis, and the strongest inhibitory eﬀect was observed after 24 h between 15 and 30 °C. Sensitivity of the tested human pathogenic bacteria against the inhibitory strains was distinct, as Y. enterocolitica could be inhibited by numerous isolates, while S. Hartford proved to be the most resistant. Our results reveal that the isolated bacteria or their excreted metabolites could hinder pathogen growth when used in suﬃcient quantities.

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The Inhibitory Effect of Lactobacillus acidophilus and Lactobacillus plantarum against Candida albicans Associated with Denture Stomatitis

Baghdad Science Journal ◽

10.21123/bsj.12.3.479-484 ◽

2015 ◽

Vol 12 (3) ◽

pp. 479-484

Author(s):

Baghdad Science Journal

Keyword(s):

Candida Albicans ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Lactobacillus Plantarum ◽

Lactobacillus Acidophilus ◽

Solid Medium ◽

Inhibition Zone ◽

Inhibitory Effect ◽

Denture Stomatitis ◽

Inhibition Effect

In this study Candida speices was diagnosed in 26 swab samples from patients with denture stomatitis , investigates the antagonism activity of Lactobacillus was investigated against the yeast of Candida albicans in vitro.Results revealed that The inhibition effect of Lactic Acid Bacteria against C.albicans was examined in solid medium, L.plantarum gave higher inhibition average 11mm followed by L.acidophillus with average 9 mm and, L.fermentum , L.casei with averages 7 mm. Whereas the filtrates, the highest inhibition zone were 20 and 16 mm by L. plantarum and L.acidophillus, respectively.

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Preventive Effect of Lactobacillus Plantarum CQPC10 on Activated Carbon Induced Constipation in Institute of Cancer Research (ICR) Mice

Applied Sciences ◽

10.3390/app8091498 ◽

2018 ◽

Vol 8 (9) ◽

pp. 1498 ◽

Cited By ~ 14

Author(s):

Jing Zhang ◽

Xianrong Zhou ◽

Benshou Chen ◽

Xingyao Long ◽

Jianfei Mu ◽

...

Keyword(s):

Nitric Oxide ◽

Activated Carbon ◽

Nitric Oxide Synthase ◽

Lactobacillus Plantarum ◽

Quantitative Polymerase Chain Reaction ◽

Inhibitory Effect ◽

Lactobacillus Bulgaricus ◽

Control Group ◽

Expression Levels ◽

Oxide Synthase

Chinese Paocai is a traditional fermented food containing an abundance of beneficial microorganisms. In this study, the microorganisms in Szechwan Paocai were isolated and identified, and a strain of lactic acid bacteria (Lactobacillus plantarum CQPC10, LP-CQPC10) was found to exert an inhibitory effect on constipation. Microorganisms were isolated and identified via 16S rDNA. Activated carbon was used to induce constipation in a mouse model and the inhibitory effect of LP-CQPC10 on this induced constipation was investigated via both pathological sections and qPCR (quantitative polymerase chain reaction). A strain of Lactobacillus plantarum was identified and named LP-CQPC10. The obtained results showed that, as compared to the control group, LP-CQPC10 significantly inhibited the amount, weight, and water content of faeces. The defecation time of the first tarry stool was significantly shorter in LP-CQPC10 groups than in the control group. The activated carbon progradation rate was significantly higher when compared to the control group and the effectiveness was improved. LP-CQPC10 increased the serum levels of MTL (motilin), Gas (gastrin), ET (endothelin), AchE (acetylcholinesterase), SP (substance P), and VIP (vasoactive intestinal peptide), while decreasing the SS (somatostatin) level. Furthermore, it improved the GSH (glutathione) level and decreased the MPO (myeloperoxidase), MDA (malondialdehyde), and NO (nitric oxide) levels. The results of qPCR indicated that LP-CQPC10 significantly up-regulated the mRNA expression levels of c-Kit, SCF (stem cell factor), GDNF (glial cell-derived neurotrophic factor), eNOS (endothelial nitric oxide synthase), nNOS (neuronal nitric oxide synthase), and AQP3 (aquaporin-3), while down-regulating the expression levels of TRPV1 (transient receptor potential cation channel subfamily V member 1), iNOS (inducible nitric oxide synthase), and AQP9 (aquaporin-9). LP-CQPC10 showed a good inhibitory effect on experimentally induced constipation, and the obtained effectiveness is superior to that of Lactobacillus bulgaricus, indicating the better probiotic potential of this strain.

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Detection of Campylobacter jejuni and Campylobacter coli from Broiler Chicken–Related Samples Using BAX PCR and Conventional International Organization for Standardization Culture

Journal of Food Protection ◽

10.4315/0362-028x-71.4.835 ◽

2008 ◽

Vol 71 (4) ◽

pp. 835-838 ◽

Cited By ~ 9

Author(s):

LISA K. WILLIAMS ◽

ALISDAIR MCMEECHAN ◽

TAMSIN BAALHAM ◽

LAURA WARD ◽

TOM J. HUMPHREY ◽

...

Keyword(s):

Campylobacter Jejuni ◽

Broiler Chicken ◽

Culture Method ◽

International Organization ◽

Campylobacter Coli ◽

Poultry Production ◽

Production Chain ◽

Enrichment Cultures ◽

International Organization For Standardization ◽

Campylobacter Spp

In this study, the conventional International Organization for Standardization (ISO) culture method was compared with the DuPont Qualicon BAX system, a high-throughput, rapid molecular assay that can be used to detect several bacterial species, including Campylobacter jejuni and Campylobacter coli in diverse sample types. Standard enrichment culture is a time-consuming process, taking up to 6 days to obtain a confirmed result. Rapid molecular assays have been developed that provide results within 24 h. Naturally contaminated samples from the poultry production chain were examined for the presence of Campylobacter spp. Samples from broiler chicken ceca (n = 100), fresh chicken carcass rinses (n = 60), and bootsocks (gauze sock walked through a broiler chicken house; n = 50) were enriched according to the ISO 10272 method in Bolton broth specifically designed to detect Campylobacter spp. in complex sample types. Samples were enriched without blood for use with the BAX system using the Campylobacter BAX kits for the detection of C. jejuni and C. coli. Samples also were directly plated onto modified charcoal cefperazone deoxycholate agar, and results were compared with those from the enriched samples for the ability to detect Campylobacter spp. Campylobacter spp. were isolated from 49% of samples with conventional enrichment cultures, from 48% with direct culture, from 68% with the BAX system and enrichment cultures, and from 62% with the BAX system used directly with samples. Overall, the BAX system detected more positive samples than did the conventional culture method and is an effective methodology for the rapid and reliable detection of Campylobacter spp. from diverse sample types.

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