First Case of Glyphosate Resistance in Bromus catharticus Vahl.: Examination of Endowing Resistance Mechanisms

Bromus catharticus Vahl. has been used as a valuable forage crop, but it has also been noted as a weed of winter crops and an invader in several countries. In Argentina, a putative glyphosate-resistant population of B. catharticus was identified as a consequence of the lack of effective control with glyphosate in the pre-sowing of wheat. Plant survival and shikimate accumulation analysis demonstrated a lower glyphosate-sensitivity of this population in comparison to a susceptible B. catharticus population. The resistant population was 4-fold more resistant to glyphosate than its susceptible counterpart. There was no evidence of target-site mechanisms of glyphosate resistance or an enhanced capacity to metabolize glyphosate in the resistant population. However, the resistant plants showed a lower foliar retention of glyphosate (138.34 μl solution g−1 dry weight vs. 390.79 μl solution g−1 dry weight), a reduced absorption of 14C-glyphosate (54.18 vs. 73.56%) and lower translocation of 14C-glyphosate from the labeled leaf (27.70 vs. 62.36%). As a result, susceptible plants accumulated a 4.1-fold higher concentration of 14C-glyphosate in the roots compared to resistant plants. The current work describes the first worldwide case of glyphosate resistance in B. catharticus. A reduced foliar retention of herbicide, a differential rate of glyphosate entry into leaves and an altered glyphosate translocation pattern would be the most likely mechanisms of glyphosate exclusion.

First Case of Multiple Resistance to EPSPS and PSI in Eleusine indica (L.) Gaertn. Collected in Rice and Herbicide-Resistant Crops in Colombia

Eleusine indica is a highly competitive and difficult-to-control plant in annual and perennial crops. In Colombia, broad-spectrum herbicides, such as paraquat and glyphosate, have begun to present poor levels of control for this weed. The multiple resistance to glyphosate and paraquat, the increase in herbicide performance with adjuvants (Retenol® and Trend® 90), and alternative herbicides were evaluated in a resistant (R) population of E. indica collected in rice fields, which is rotated with herbicide-resistant (HR) crops. Based on plant mortality, the R population was 9.8 and 7.2 times more resistant than susceptible (S) plants to glyphosate and paraquat, respectively. R plants accumulated 4.2 less shikimic acid and had at least 70% less electrolyte leakage than S plants when they were exposed to glyphosate and paraquat, respectively. Both adjuvants increased the foliar retention of herbicides. In addition, adjuvants also increased the performance of glyphosate effectiveness between 22% and 58% and that of paraquat from 61% to 100%. Alternative herbicides (atrazine, clethodim, imazamox, diuron, flazasulfuron, glufosinate, oxyfluorfen, quizalofop, and tembotrione) provided high levels of control in both populations of E. indica. This is the first case of multiple resistant E. indica confirmed in Colombia. Adjuvants improved the leaf retention and efficacy of glyphosate and paraquat. In summary, the alternative herbicides evaluated in this study should be adopted by Colombian farmers and provide additional herbicide modes-of-action to combat future resistance.

Aphelenchoides besseyi Parasitizing Common Bean in Brazil

Aphelenchoides besseyi is the causal agent of soybean green stem and foliar retention syndrome known as Soja Louca II. This nematode has recently been reported parasitizing cotton in Brazil. In Costa Rica, it causes the symptoms known as “amachamiento” and false angular spots in common bean (Phaseolus vulgaris). Due to the great importance of beans to Brazilian agriculture, the objective of this research was to study the pathogenicity of A. besseyi in common bean under greenhouse conditions, including its endoparasitic relationships by staining root and shoot system tissues with fuchsin acid. In addition, A. besseyi was collected and quantified from shoot systems 30 days after inoculation by washing the tissue in water and blender centrifugal-flotation. We observed the symptoms of “amachamiento”, leaf and vein deformation in the expanded trifoliate leaves, and also leaves with necrotic, brown to reddish and angular lesions, characteristics from false angular spot, and deformed stems characterized by enlargement of nodes, retortions and necrotic lesions. High numbers of nematodes were found inside common bean plants. This is the first report of the pathogenicity and symptoms caused by A. besseyi in common bean in Brazil. These findings are important for development of management strategies to avoid losses on bean cropped in infested areas.

Measurement of foliar spray retention on creeping bentgrass

Experiments were conducted to evaluate the impact of spray volume, nozzle type, adjuvants, the presence of dew, and their interactions on foliar retention of creeping bentgrass. Tartrazine, a common food dye, was used as a tracer in this study. Increasing spray volume from 95 L ha−1 to 1,500 L ha−1 decreased foliar retention efficiency from 98% to approximately 85%. Compared with flat-fan nozzles, air-induction nozzles delivered similar retention efficiency at all spray volumes evaluated. However, flat-fan nozzles provided higher uniformity and more thorough coverage. Adding nonionic surfactants, organosilicone adjuvants, or methylated seed oils at typical concentrations yielded retention efficiency of approximately 90% to 93% regardless of spray volumes. In contrast, with water alone, increasing spray volume reduced retention efficiency from 95.9% to 87.3%. Simulated dew applied at 1,950 L ha−1 increased retention efficiency by approximately 3% when spray application volume was 190 L ha−1, while no difference was observed at 750 L ha−1. The presence of dew reduced the impact of adjuvants on retention efficiency. Large quantities of dew, 3,800 L ha−1, did reduce retention efficiency.

Tannic acid-based nanopesticides coating with highly improved foliage adhesion to enhance foliar retention

Poor utilization of conventional pesticides has resulted in overuse, which increases cost, toxicity to other non-target organisms, concerns about human health and safety, groundwater and contamination, with ecosystem destruction and food pollution.

Development of functionalized abamectin poly(lactic acid) nanoparticles with regulatable adhesion to enhance foliar retention

Functionalized abamectin poly(lactic acid) nanoparticles with different adhesion abilities to crop foliage can regulate folia retention of abamectin.