One-step pipetting of barcoded planar microparticles into compact monolayer assembling chip for efficient readout of multiplexed immunoassay

Barcoded planar microparticles have many qualities suitable for developing cost-efficient multiplexed immunoassays. But at the translational research level, there are a number of technical aspects yet remain to be addressed which includes robustness and efficiency of the assay readout process. Assay readout process involves automated barcode identification and signal intensity values from each planar microparticle. For this, each microparticle has to be correctly aligned for correct barcode readout while being, ideally, compactly assembled for maximum microparticle imaging efficiency. To simultaneously achieve such alignment and assembly of microparticles but in a straightforward manner, we designed a microfluidic microparticle assembling chip that only requires a single pipetting step. Our design utilizes capillary flow based guided particle assembly, which allows maximum microparticle-based immunoassay readout efficiency. With the aid of image processing algorithms, we obtained good multiplex immunoassay readout accuracy similar to conventional imaging platforms. Our approach is applicable to both soft elastomer materials (e.g. PDMS) and rigid materials (e.g. polystyrene), the latter of which is frequently used for injection molding based mass production. We anticipate our device could help developing facile and user-friendly platform technologies based on barcoded planar microparticles.

Engineering deformation-free plastic spiral inertial microfluidic system for CHO cell clarification in biomanufacturing

Inertial microfluidics has enabled many impactful high throughput applications. However, devices fabricated in soft elastomer (i.e., polydimethylsiloxane (PDMS)) suffer reliability issues due to significant deformation generated by the high pressure...

Stretchable Strain Sensor with Controllable Negative Resistance Sensitivity Coefficient Based on Patterned Carbon Nanotubes/Silicone Rubber Composites

In this paper, stretchable strain sensors with a controllable negative resistance sensitivity coefficient are firstly proposed. In order to realize the sensor with a negative resistance sensitivity coefficient, a stretchable stress sensor with sandwich structure is designed in this paper. Carbon nanotubes are added between two layers of silica gel. When the sensor is stretched, carbon nanotubes will be squeezed at the same time, so the sensor will show a resistance sensitivity coefficient that the resistance becomes smaller after stretching. First, nanomaterials are coated on soft elastomer, then a layer of silica gel is wrapped on the outside of the nanomaterials. In this way, similar to sandwich biscuits, a stretchable strain sensor with controllable negative resistance sensitivity coefficient has been obtained. Because the carbon nanotubes are wrapped between two layers of silica gel, when the silica gel is stretched, the carbon nanotubes will be squeezed longitudinally, which increases their density and resistance. Thus, a stretchable strain sensor with negative resistance sensitivity coefficient can be realized, and the resistivity can be controlled and adjusted from 12.7 Ω·m to 403.2 Ω·m. The sensor can be used for various tensile testing such as human motion monitoring, which can effectively expand the application range of conventional tensile strain sensor.

Non-close-packed arrangement of soft elastomer microspheres on solid substrates

Unlike rigid microparticles, deformable elastomer microspheres were found to exhibit a non-close-packed arrangement on solid substrates after evaporating water from their dispersions. The underlying mechanism of their unique ordering is discussed.

Гистерезис в адгезионном контакте при изменении направления сдвига: эксперимент и феноменологическая модель

A phenomenological model is proposed that describes hysteretic behavior of an adhesive contact between a soft elastomer and a rigid indenter when the direction of the indenter shear is changed. The model takes into account the increase of contact strength with contact time. The dependences of the elastic force and contact radius on the indentation depth were obtained. It is shown that the adhesive strength of the contact increases with an increase in the indentation depth. An experiment on the indentation of a steel spherical indenter into a rubber sheet of a fixed thickness was carried out. A qualitative agreement between the experimental and theoretical results is shown.