scholarly journals One-step pipetting of barcoded planar microparticles into compact monolayer assembling chip for efficient readout of multiplexed immunoassay

2022 ◽  
Author(s):  
Sangwook Bae ◽  
Daewon Lee ◽  
Hunjong Na ◽  
Jiseong Jang ◽  
Sunghoon Kwon
Keyword(s):  
Capillary Flow ◽  
Straightforward Manner ◽  
Particle Assembly ◽  
Research Level ◽  
One Step ◽  
Image Processing Algorithms ◽  
Cost Efficient ◽  
Multiplexed Immunoassay ◽  
User Friendly ◽  
Soft Elastomer

Barcoded planar microparticles have many qualities suitable for developing cost-efficient multiplexed immunoassays. But at the translational research level, there are a number of technical aspects yet remain to be addressed which includes robustness and efficiency of the assay readout process. Assay readout process involves automated barcode identification and signal intensity values from each planar microparticle. For this, each microparticle has to be correctly aligned for correct barcode readout while being, ideally, compactly assembled for maximum microparticle imaging efficiency. To simultaneously achieve such alignment and assembly of microparticles but in a straightforward manner, we designed a microfluidic microparticle assembling chip that only requires a single pipetting step. Our design utilizes capillary flow based guided particle assembly, which allows maximum microparticle-based immunoassay readout efficiency. With the aid of image processing algorithms, we obtained good multiplex immunoassay readout accuracy similar to conventional imaging platforms. Our approach is applicable to both soft elastomer materials (e.g. PDMS) and rigid materials (e.g. polystyrene), the latter of which is frequently used for injection molding based mass production. We anticipate our device could help developing facile and user-friendly platform technologies based on barcoded planar microparticles.

scholarly journals Smart Secured Real Time Agriculture Monitoring System

2018 ◽  
Vol 7 (3.6) ◽  
pp. 281
Author(s):  
J Srirampavan
Keyword(s):  
Sensor Networks ◽  
Embedded Systems ◽  
Sensor Network ◽  
Mobile Device ◽  
Vital Role ◽  
Separate Entity ◽  
Cloud Data ◽  
Lcd Display ◽  
Cost Efficient ◽  
User Friendly

Embedded systems in Agriculture play a vital role in unifying the work involved and improve conservations. Designing a smart as well as a cost efficient and more user-friendly system will be idealistic challenge. The following system that has been proposed is designed with those ideal constraints in mind. It consists of a Raspberry pi3 as a gateway that links the sensor networks with the cloud. To improve security an MQTT protocol is used for cloud connectivity. The communication between the sensor networks is managed by NRF24L01. The Sensor network is a separate entity that can used like a plug and play device and is built by a micro controller with a LCD display and an interfaced GPS. Multicasting is also possible between sensor networks and the gateway. The processed data from the sensor networks is sent through NRF24L01 to the gateway. The gateway further processes and encapsulates the data and through MQTT the data gets stored on the cloud. This cloud data can be accessed through computer or mobile device  

A Practical and User-Friendly Method for the Selenium-Free One-Step Preparation of 1,2-Diketones and their Monoxime Analogs

Synlett ◽  
2004 ◽  
pp. 2315-2318 ◽  
Author(s):  
Georg Rüedi ◽  
Matthias A. Oberli ◽  
Matthias Nagel ◽  
Christophe Weymuth ◽  
Hans-Jürgen Hansen
Keyword(s):  
One Step ◽  
User Friendly

scholarly journals Virus particle assembly into crystalline domains enabled by the coffee ring effect

Soft Matter ◽  
2014 ◽  
Vol 10 (30) ◽  
pp. 5458-5462 ◽  
Author(s):  
Ronald Gebhardt ◽  
Jean-Marie Teulon ◽  
Jean-Luc Pellequer ◽  
Manfred Burghammer ◽  
Jacques-Philippe Colletier ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Particle ◽  
Contact Line ◽  
Capillary Flow ◽  
Particle Assembly ◽  
Virus Particles ◽  
Coffee Ring ◽  
Coffee Ring Effect ◽  
Crystalline Domains

Tobacco mosaic virus particles can be rapidly assembled into 3D-domains by capillary flow-driven alignment at the triple contact-line of an evaporating droplet.

scholarly journals ImageM: a user-friendly interface for the processing of multi-dimensional images with Matlab

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 333
Author(s):  
David Legland ◽  
Marie-Françoise Devaux
Keyword(s):  
Image Processing ◽  
Image Analysis ◽  
User Interface ◽  
Image Data ◽  
Image Visualization ◽  
Image Processing Algorithms ◽  
Processing Algorithms ◽  
Spatial Calibration ◽  
User Friendly ◽  
Efficient Software

Modern imaging devices provide a wealth of data often organized as images with many dimensions, such as 2D/3D, time and channel. Matlab is an efficient software solution for image processing, but it lacks many features facilitating the interactive interpretation of image data, such as a user-friendly image visualization, or the management of image meta-data (e.g. spatial calibration), thus limiting its application to bio-image analysis. The ImageM application proposes an integrated user interface that facilitates the processing and the analysis of multi-dimensional images within the Matlab environment. It provides a user-friendly visualization of multi-dimensional images, a collection of image processing algorithms and methods for analysis of images, the management of spatial calibration, and facilities for the analysis of multi-variate images. ImageM can also be run on the open source alternative software to Matlab, Octave. ImageM is freely distributed on GitHub: https://github.com/mattools/ImageM.

scholarly journals ZAF – An Open Source Fully Automated Feeder for Aquatic Facilities

2021 ◽  
Author(s):  
Merlin Lange ◽  
AhmetCan Solak ◽  
Shruthi Vijay Kumar ◽  
Hirofumi Kobayashi ◽  
Bin Yang ◽  
...  
Keyword(s):  
Open Source ◽  
Food Distribution ◽  
Model Organisms ◽  
Precise Control ◽  
Automatic Feeder ◽  
Animal Feeding ◽  
Fully Automatic ◽  
Cost Efficient ◽  
User Friendly ◽  
Advanced Version

In the past few decades, aquatic animals have become popular model organisms in biology, spurring a growing need for establishing aquatic facilities. Zebrafish are widely studied and relatively easy to culture using commercial systems. However, a challenging aspect of maintaining aquatic facilities is animal feeding, which is both time- and resource-consuming. We have developed an open-source fully automatic daily feeding system, Zebrafish Automatic Feeder (ZAF). ZAF is reliable, provides a standardized amount of food to every tank, is cost-efficient, easy to build, and has a user-friendly interface. The advanced version, ZAF+, allows for the precise control of food distribution as a function of fish density per tank. Both ZAF and ZAF+ are adaptable to any laboratory environment and can help facilitate the implementation of aquatic colonies. Here we provide all blueprints and instructions for building the mechanics, electronics, fluidics, as well as to setup the control software and its user-friendly graphical interface. Importantly, the design is modular and can be scaled to meet different user needs. Furthermore, our results show that ZAF and ZAF+ do not adversely affect zebrafish culture, enabling fully automatic feeding for any aquatic facility.

scholarly journals LotuS2: An ultrafast and highly accurate tool for amplicon sequencing analysis

2021 ◽  
Author(s):  
Ezgi Ozkurt ◽  
Joachim Fritscher ◽  
Nicola Soranzo ◽  
Duncan Y.K. Ng ◽  
Robert P. Davey ◽  
...  
Keyword(s):  
Data Analysis ◽  
Clustering Algorithms ◽  
Amplicon Sequencing ◽  
Sequencing Analysis ◽  
Alpha And Beta Diversity ◽  
High Data ◽  
Data Usage ◽  
Long Read ◽  
Cost Efficient ◽  
User Friendly

Background: Amplicon sequencing is an established and cost-efficient method for profiling microbiomes. However, many available tools to process this data require both bioinformatics skills and high computational power to process big datasets. Furthermore, there are only few tools that allow for long read amplicon data analysis. To bridge this gap, we developed the LotuS2 (Less OTU Scripts 2) pipeline, enabling user-friendly, resource friendly, and versatile analysis of raw amplicon sequences. Results: In LotuS2, six different sequence clustering algorithms as well as extensive pre- and post-processing options allow for flexible data analysis by both experts, where parameters can be fully adjusted, and novices, where defaults are provided for different scenarios. We benchmarked three independent gut and soil datasets, where LotuS2 was on average 29 times faster compared to other pipelines - yet could better reproduce the alpha- and beta-diversity of technical replicate samples. Further benchmarking a mock community with known taxa composition showed that, compared to the other pipelines, LotuS2 recovered a higher fraction of correctly identified genera and species (98% and 57%, respectively). At ASV/OTU level, precision and F-score were highest for LotuS2, as was the fraction of correctly reconstructed 16S sequences. Conclusion: LotuS2 is a lightweight and user-friendly pipeline that is fast, precise and streamlined. High data usage rates and reliability enable high-throughput microbiome analysis in minutes. Availability: LotuS2 is available from GitHub, conda or via a Galaxy web interface, documented at http://lotus2.earlham.ac.uk/.

scholarly journals ZAF, the first open source fully automated feeder for aquatic facilities

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Merlin Lange ◽  
AhmetCan Solak ◽  
Shruthi Vijay Kumar ◽  
Hirofumi Kobayashi ◽  
Bin Yang ◽  
...  
Keyword(s):  
Open Source ◽  
Food Distribution ◽  
Model Organisms ◽  
Precise Control ◽  
Automatic Feeder ◽  
Animal Feeding ◽  
Fully Automatic ◽  
Cost Efficient ◽  
User Friendly ◽  
Advanced Version

In the past few decades, aquatic animals have become popular model organisms in biology, spurring a growing need for establishing aquatic facilities. Zebrafish are widely studied and relatively easy to culture using commercial systems. However, a challenging aspect of maintaining aquatic facilities is animal feeding, which is both time- and resourceconsuming. We have developed an open-source fully automatic daily feeding system, Zebrafish Automatic Feeder (ZAF). ZAF is reliable, provides a standardized amount of food to every tank, is cost-efficient and easy to build. The advanced version, ZAF+, allows for the precise control of food distribution as a function of fish density per tank, and has a user-friendly interface. Both ZAF and ZAF+ are adaptable to any laboratory environment and facilitate the implementation of aquatic colonies. Here we provide all blueprints and instructions for building the mechanics, electronics, fluidics, as well as to setup the control software and its user-friendly graphical interface. Importantly, the design is modular and can be scaled to meet different user needs. Furthermore, our results show that ZAF and ZAF+ do not adversely affect zebrafish culture, enabling fully automatic feeding for any aquatic facility.

scholarly journals Design and Fabrication of Beach Cleaning Machine

2019 ◽  
Vol 8 (12) ◽  
pp. 1597-1602
Keyword(s):  
Environmental Pollution ◽  
Marine Environment ◽  
High Efficiency ◽  
Cleaning Machine ◽  
Human Effort ◽  
Cost Efficient ◽  
User Friendly ◽  
D Model

The beach cleaning machine is a vehicle that can be used to pick up rags from the shores thus keeping the beaches clean. A great number of people visit beaches very often for relaxation and recreation. People throw plastics quite often on shores without knowing the consequences. This results in environmental pollution which affects the marine environment and increases the risks of diseases. In order to overcome this we have designed and fabricated a beach cleaning machine that will clean the beaches by picking up rags with minimal human effort. Here, we have incorporated an Arduino board and developed an app through which we will be able to control the operations of the machine within a range of 10 meter through Bluetooth. A 3-D model of the design has been developed and analysis has been performed. The machine developed is a cost efficient vehicle with high efficiency and also user friendly

scholarly journals Labeling and Probing the Silica Surface Using Mechanochemistry and 17O NMR Spectroscopy

2021 ◽  
Author(s):  
Chia-Hsin Chen ◽  
Frederic Mentink-Vigier ◽  
Julien Trébosc ◽  
Ieva Goldberga ◽  
Philippe Gaveau ◽  
...  
Keyword(s):  
Fumed Silica ◽  
Silica Surface ◽  
17O Nmr ◽  
Surface Information ◽  
Clear Identification ◽  
Common Interface ◽  
Cost Efficient ◽  
User Friendly ◽  
First Time ◽  
17O Nmr Spectroscopy

In recent years, there has been increasing interest in developing cost-efficient, fast, and user-friendly <sup>17</sup>O enrichment protocols to help understand the structure and reactivity of materials using <sup>17</sup>O NMR. Here, we show for the first time how ball milling (BM) can be used to selectively and efficiently enrich the surface of fumed silica, which is widely used at the industrial scale. Short milling times (up to 15 min) allowed modulation of the enrichment level (up to ca. 5%) without significantly changing the nature of the material. High-precision <sup>17</sup>O-compositions were measured at different milling times using LG-SIMS. High-resolution <sup>17</sup>O NMR analyses (including at 35.2 T) allowed clear identification of the signals from siloxane (Si-O-Si) and silanols (Si-OH), while DNP analyses, performed using direct <sup>17</sup>O polarization and indirect <sup>17</sup>O{<sup>1</sup>H} CP excitation, agreed with selective<sup> </sup>labeling of the surface. Information on the distribution of Si-OH environments at the surface was obtained from 2D <sup>1</sup>H-<sup>17</sup>O D-HMQC correlations. Finally, the surface-labeled silica was reacted with titania and using <sup>17</sup>O DNP, their common interface was probed and Si-O-Ti bonds identified.

The Way to High Volume Fabrication of Lab-on-a-Chip Devices—A Technological Approach for Polymer Based Microfluidic Systems with Integrated Active Valves and Pumps

2009 ◽  
Vol 6 (4) ◽  
pp. 198-204 ◽  
Author(s):  
Jochen Rupp ◽  
Manuela Schmidt ◽  
Bettina Günther ◽  
Michael Stumber ◽  
Sven Zinober ◽  
...  
Keyword(s):  
Mechanical Stability ◽  
Thermoplastic Elastomer ◽  
High Volume ◽  
Active Components ◽  
Passive Components ◽  
Process Step ◽  
Technological Approach ◽  
Pump Rate ◽  
One Step ◽  
Cost Efficient

We present a technology platform suitable for the mass production of laboratory-on-a-chip devices made of polymers with integrated active and passive components. The presented microfluidic platform with integrated valves and pumps for active flow management is realized with three layers consisting of two polymer parts separated by a thin elastic TPE (thermoplastic elastomer) membrane welded together in one step. The elastic TPE membrane acts as an integrated deflectable membrane layer between the two outer polymer layers, each made of a weldable thermoplastic polymer (polycarbonate). Valving is realized by applying pressure in a displacement chamber above a hydraulic channel causing the membrane to deform and to seal the channel. A pump is fabricated using a displacement chamber with a valve on the inlet and outlet. The presented components, namely valve and pump, show excellent behavior regarding response time, sealing quality, and pump rate needing only a low actuation pressure. The three-layer-stack is joined in a single process step by using laser welding, creating devices with high mechanical stability. This production technology fulfills the requirements of a high volume fabrication at high quality and has the potential to manufacture cost-efficient and reliable laboratory-on-a-chip systems. The used materials show a high chemical resistance against a broad range of commonly used liquids and good optical characteristics for the use in μTAS. This consistent technological approach represents a flexible platform for microfluidics with active components to be used in complex applications.

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