Mitochondrial Genomes of the Genus Claassenia (Plecoptera: Perlidae) and Phylogenetic Assignment to Subfamily Perlinae

Mitochondrial genomes of three stoneflies, e.g., Claassenia magna Wu, 1948, Claassenia sp. 2 and Claassenia xucheni Chen, 2019 were sequenced in this study with 15,774, 15,777 and 15,746 bp in length, respectively. Each mitogenome contained 37 genes including 22 tRNAs, two ribosomal RNAs, 13 protein-coding genes (PCGs), and a noncoding control region (CR). In general, standard ATN start and TAN termination codons were evident in the PCGs. Although the dihydrouridine arm was absent in trnSer, the remaining 21 tRNAs displayed the characteristic cloverleaf secondary structure. Stem-loop structures were identified in the CRs of all three mitogenomes, but tandem repeats were only apparent in Claassenia xucheni. The mitogenomes of three Claassenia species were analyzed and compared with mitogenomes in 21 other stoneflies from the Perlidae and three Euholognatha species (Rhopalopsole bulbifera, Capnia zijinshana and Amphinemura longispina) as outgroups. Phylogenetic analyses using maximum likelihood and Bayesian inference. Phylogenetic analysis supported that Claassenia was recovered as the sister group of other Perlinae and Claassenia+Perlinae emerged from the paraphyletic Acroneuriinae. The final results supported that Claassenia was classified into subfamily Perlinae and proposed Claassenia represent a transitional group of the subfamilies Acroneuriinae and Perlinae. This study provided new molecular evidence for exploring the debatable taxonomic position of the genus Claassenia in Perlidae.

Organizational, Economic, and Strategic Aspects of Capitalization of Multipurpose Forest Industries

Introduction. The present article forecasts the organizational, economic, and strategic aspects of the multifunctional forestry capitalization in the Kemerovo region aka Kuzbass. The authors analyzed the current Russian laws in order to answer the following question: is multi-purpose forest utilization able to ensure the rights and interests of all its participants, while providing legal means to resolve various related issues? Study objects and methods. The research featured the legal norms in the field of forest, wildlife, and subsoil utilization. The authors assessed their ability to guarantee sustainable use of natural resources while protecting the rights and legitimate interests of forest users. The study was based on general standard methods of cognition and special legal methods. Results and discussion. Hunting is regulated by hunting sector agreements. The Forest Code and the Hunting Law of the Russian Federation do not prohibit or restrict other types of forest utilization of hunting grounds. Most Western European countries link land ownership to hunting rights, which makes landowners liable for damage caused by hunting and obliged to protect the local fauna. Conclusion. Russian legislation does not provide for direct conciliation and compensation mechanisms in cases a part of hunting ground is used for other purposes, e.g. mining. Russian legislation needs legal termination procedures for hunting sector agreements and compensation rules in case a land plot was seized from hunting providers for subsoil use.

Standardization of Simplicia and Ethanol Extract of Purun Danau (Lepironia articulata (Retz.) Domin) Rhizome

Purun Danau (Lepironia articulata (Retz.) Domin) has been shown to have antimalarial and antioxidant activity. This study aimed to standardize simplicia and extract of L. articulata by determining the value of its specific and non-specific parameters. A sampling of L. articulata rhizome was carried out in Guntung Manggis, Haur Gading, and Halat. The standardization method used was based on the Indonesian Herbal Pharmacopoeia and the General Standard Parameter of Extract. The organoleptic observations showed that L. articulata simplicia was reddish-brown in color, chelated taste, and had a specific odor. Microscopic observation showed parts of this plant: epidermis, cortex, endodermis, parenchyma, bundle vessels, and scalariform vessels. Ethanol-soluble extract content was 10.00-12.66%, water-soluble extract content 8.03-10.87%, drying shrinkage 7.10-7.33%, total ash content 2.03-2.52%, acid-insoluble ash 0.33-0.42%, Pb content 5.698-9.989 ppm, Cd content 0.300-0.500 ppm, Hg content 0.070-0.090 ppm. Ethanol extract of L. articulata rhizome contained alkaloids, flavonoids, tannins, glycosides, and saponins. The yield obtained was 8.05-11.23%, total ash content was 1.58-1.67%, acid-insoluble ash was 0.23-0.33%, and water content was 7.10-8.50%. Standardization of simplicia and ethanol extract of L. articulata rhizome has met the criteria.

WINNING AT ALL COSTS TOO COSTLY S v Rozani; Rozani v Director of Public Prosecutions, Western Cape 2009 1 SACR 540 (C)

The role of the public prosecutor is one to be respected. Members of society expect to enjoy lives free of violence, theft and other criminal violation; in return, they surrender the exercise of “vengeance” and vigilantism to the state. The public prosecutor (inter alia) is entrusted with the duty of ensuring that justice is served in bringing transgressors to book. The public prosecutor thus has the onerous task of ensuring that the rights of victims are served and given a voice, but at the same time doing so in a manner which upholds the basic tenets of a free, fair and just society. The duty and role of the defence attorney (state appointed or otherwise) ismuch the same. He or she is expected also to serve justice by giving his or her client (paying or pro bono) the best service and defence he or she is capable of. Obviously, this does not mean conjuring up or “manifesting” a defence. But he or she must, at the very least, prevent his or her client from pleading guilty to an offence where one was not committed. The recent decision in Rozani (2009 1 SACR 540 (C)) makes it evident that the fulfilment of such goals and ideals is not easy. The legal profession has gained a rather dubious reputation, attracting epithets such as “con-artist”, “shyster”, “opportunist” and “shark”, amongst others. The perception that individuals join the profession only to make a “quick buck” has stuck and the case at hand certainly seems to show this, reflecting not only a callous disregard for justice, but also what is blatant incompetence on the part of both the prosecutor and the defence attorney. Reading the facts of the matter, one wonders about the general standard of lawyers entering the profession – one cannot but marvel at the farcical aspect of the facts in Rozani. The main objective of practitioners within a criminal justice system should not be to win at all costs, but rather to ensure that justice is served. The facts leading up to the review in Rozani reflect the prosecutor’s need to chalk up wins and the defence attorney’s need to meet fee targets at whatever cost. The decision and remarks from the bench form a sobering commentary on the state of the criminal courts and the pursuit of justice in South Africa. While the level of crime in this country bolsters the need to convict criminals, this provides no excuse for disregarding the basic tenets of justice.

6. Breach of duty

This chapter discusses the law on standard of care and breach of duty. To establish that the duty of care has been breached, the standard of care must first be found and then it must be decided if that standard was reached in the circumstances. The general standard of care is objective: the ‘reasonable person’ standard. Variations in the standard of care regarding children and the more skilled or professional are discussed, as are those pertaining to sport and the medical profession. Proof of breach must be established by the claimant on the balance of probabilities; occasionally with the benefit of the evidential tool of res ipsa loquitur.

Analisis Perputaran Persediaan Dalam Menilai Return On Asset (ROA) Pada PT. Kalbe Farma, Tbk Periode 2015 - 2019

This study aims to determine how inventory turnover is in assessing Return On Asset. The research object, namely PT. Kalbe Farma, Tbk, which is taken from the financial reports for the period 2015-2019. This study used a qualitative method consisting of two variables, namely inventory cycle as the independent variable and return on asset the dependent variable. The data analysis method used in this research is inventory turnover analysis and return on asset analysis. The test results show that the average inventory turnover of 3.15 per year as a whole is categorized as good, when viewed from the general standard or the industry average which is above 2.5 times. For the average return on asset, it shows that return on asset is included in the very good criteria because it has reached the company standard. Then from the comparison of inventory turnover and return on assetor the last 5 years of the 2015-2019 period, the contribution of inventory turnover in assessing return on asset was 21.28% and the rest contributed to other factors not examined in this study. Penelitian ini bertujuan untuk mengetahui bagaimana perputaran persediaan dalam menilai Return On Asset. Objek penelitian yaitu PT. Kalbe Farma, Tbk yang diambil dari laporan keuangan periode 2015-2019. Penelitian ini menggunakan metode kualitatif yang terdiri dari dua variabel yaitu siklus persediaan sebagai variabel bebas dan return on asset sebagai variabel terikat. Metode analisis data yang digunakan dalam penelitian ini adalah analisis perputaran persediaan dan analisis return on asset. Hasil pengujian menunjukkan bahwa rata-rata perputaran persediaan 3,15 per tahun secara keseluruhan dikategorikan baik, jika dilihat dari standar umum atau rata-rata industri yang berada di atas 2,5 kali. Untuk rata-rata return on asset menunjukkan bahwa return on asset termasuk dalam kriteria sangat baik karena telah mencapai standar perusahaan. Kemudian dari perbandingan perputaran persediaan dan return on assetor 5 tahun terakhir periode 2015-2019, kontribusi perputaran persediaan dalam menilai return on asset adalah 21,28% dan sisanya berkontribusi pada faktor lain yang tidak diteliti dalam penelitian ini.

CREATING A REGISTER OF SEXUAL OFFENDERS AND THE PROBLEM OF SEIZURE OF A CONVICTION IN THE CONTEXT OF ART. 8 OF THE ECHR

The present analysis aims to present the general standard of the ECtHR with regard to the creation and functioning of a register of criminals, especially sex offenders. The analysis attempts to answer the question of what requirements the state should meet in order to implement the ECtHR standard in this matter. For this reason, attention has been paid to the nature of the protection measure in question, the manner of its implementation, as well as the material scope and conditions of its application.

Epigenetic dysregulation in myeloid malignancies

Epigenetic deregulation is now a well-recognized -though not yet fully understood- mechanism that contributes to the development and progression of myeloid malignancies. In the past 15 years, next generation sequencing studies have revealed patterns of aberrant DNA methylation, altered chromatin states, and mutations in chromatin modifiers across the spectrum of myeloid malignancies. Studies into the mechanisms that drive these diseases through mouse modeling have helped identify new avenues for therapeutic interventions, from initial treatment to resistant, relapsed disease. This is particularly significant when chemotherapy with cytotoxic agents remains the general standard of care. In this review, we will discuss some of the recent findings of epigenetic mechanisms and how these are informing the development of more targeted strategies for therapeutic intervention in myeloid malignancies.