Riverine impact on future projections of marine primary production and carbon uptake

Riverine transport of nutrients and carbon from inland waters to the coastal and finally the open ocean alters marine primary production (PP) and carbon (C) uptake, not only regionally but also globally. So far, this contribution is represented in the state-of-the-art Earth system models with limited effort. Here we assess changes in marine PP and C uptake projected under the Representative Concentration Pathway 4.5 climate scenario using the Norwegian Earth system model, with four riverine configurations: deactivated, fixed at a contemporary level, coupled to simulated freshwater runoff, and following four plausible future scenarios. The inclusion of riverine nutrients and carbon improves the modelled contemporary spatial distribution relative to observations, especially on the continental margins (5.4 % reduction in root mean square error [RMSE] for PP) and in the North Atlantic region (7.4 % reduction in RMSE for C uptake). Riverine nutrient inputs alleviate nutrient limitation, especially under future warmer conditions as stratification increases, and thus lessen the projected future decline in PP by up to 0.6 PgC yr−1 (27.3 %) globally depending on the riverine configuration. The projected C uptake is enhanced along continental margins where increased PP, due to riverine nutrient inputs, dominates over the CO2 outgassing owing to riverine organic matter inputs. Conversely, where the riverine organic matter inputs dominate over the nutrient inputs, the projected C uptake is reduced. The large range of the riverine input across our four riverine configurations does not transfer to a large uncertainty of the projected global PP and ocean C uptake, suggesting that transient riverine inputs are more important for high-resolution regional studies such as in the North Atlantic and along the continental margins.

Genomic evidence for inter-class host transition between abundant streamlined heterotrophs by a novel and ubiquitous marine Methylophage

The methylotrophic OM43 clade are Gammaproteobacteria that comprise some of the smallest free-living cells known with highly streamlined genomes. OM43 represents an important microbial link between marine primary production and return of carbon back to the atmosphere. Bacteriophages shape microbial communities and are major drivers of mortality and global marine biogeochemistry. Recent cultivation efforts have brought the first viruses infecting members of the OM43 clade into culture. Here we characterize a novel myophage infecting OM43, called Melnitz. Melnitz was isolated independently on three separate occasions (with isolates sharing >99.95% average nucleotide identity) from water samples from a subtropical ocean gyre (Sargasso Sea) and temperate coastal (Western English Channel) systems. Metagenomic recruitment from global ocean viromes confirmed that Melnitz is globally ubiquitous, congruent with patterns of host abundance. Bacteria with streamlined genomes such as OM43 and the globally dominant SAR11 clade use riboswitches as an efficient method to regulate metabolism. Melnitz encodes a two-piece tmRNA (ssrA), controlled by a glutamine riboswitch, providing evidence that riboswitch use also occurs for regulation during phage infection of streamlined heterotrophs. Virally encoded tRNAs and ssrA found in Melnitz were phylogenetically more closely related to those found within the alphaproteobacterial SAR11 clade and their associated myophages than those within their gammaproteobacterial hosts. This suggests the possibility of an ancestral inter-class host transition event between SAR11 and OM43. Melnitz and a related myophage that infects SAR11 were unable to infect hosts of the SAR11 and OM43, respectively, suggesting host transition rather than a broadening of host range.

Global distribution patterns of marine nitrogen-fixers by imaging and molecular methods

Nitrogen fixation has a critical role in marine primary production, yet our understanding of marine nitrogen-fixers (diazotrophs) is hindered by limited observations. Here, we report a quantitative image analysis pipeline combined with mapping of molecular markers for mining >2,000,000 images and >1300 metagenomes from surface, deep chlorophyll maximum and mesopelagic seawater samples across 6 size fractions (<0.2–2000 μm). We use this approach to characterise the diversity, abundance, biovolume and distribution of symbiotic, colony-forming and particle-associated diazotrophs at a global scale. We show that imaging and PCR-free molecular data are congruent. Sequence reads indicate diazotrophs are detected from the ultrasmall bacterioplankton (<0.2 μm) to mesoplankton (180–2000 μm) communities, while images predict numerous symbiotic and colony-forming diazotrophs (>20 µm). Using imaging and molecular data, we estimate that polyploidy can substantially affect gene abundances of symbiotic versus colony-forming diazotrophs. Our results support the canonical view that larger diazotrophs (>10 μm) dominate the tropical belts, while unicellular cyanobacterial and non-cyanobacterial diazotrophs are globally distributed in surface and mesopelagic layers. We describe co-occurring diazotrophic lineages of different lifestyles and identify high-density regions of diazotrophs in the global ocean. Overall, we provide an update of marine diazotroph biogeographical diversity and present a new bioimaging-bioinformatic workflow.

First Evidence of the Toxin Domoic Acid in Antarctic Diatom Species

The Southern Ocean is one of the most productive ecosystems in the world. It is an area heavily dependent on marine primary production and serving as a feeding ground for numerous seabirds and marine mammals. Therefore, the phytoplankton composition and presence of toxic species are of crucial importance. Fifteen monoclonal strains of Pseudo-nitzschia subcurvata, a diatom species endemic to the Southern Ocean, were established, which were characterized by morphological and molecular data and then analysed for toxin content. The neurotoxins domoic acid and iso-domoic acid C were present in three of the strains, which is a finding that represents the first evidence of these toxins in strains from Antarctic waters. Toxic phytoplankton in Antarctic waters are still largely unexplored, and their effects on the ecosystem are not well understood. Considering P. subcurvata’s prevalence throughout the Southern Ocean, these results highlight the need for further investigations of the harmful properties on the Antarctic phytoplankton community as well as the presence of the toxins in the Antarctic food web, especially in the light of a changing climate.

Genome Sequences of Synechococcus sp. Strain MIT S9220 and Cocultured Cyanophage SynMITS9220M01

ABSTRACT Synechococcus bacteria are unicellular cyanobacteria that contribute significantly to global marine primary production. We report the nearly complete genome sequence of Synechococcus sp. strain MIT S9220, which lacks the nitrate utilization genes present in most marine Synechococcus genomes. Assembly also produced the complete genome sequence of a cyanophage present in the MIT S9220 culture.

Arctic sea-ice decline impacts on primary production

&lt;p&gt;Arctic food webs are short and relatively species poor, rendering them vulnerable to changes or perturbations at any individual trophic level. High-latitude warming represents one major source of potential perturbation to Arctic marine and terrestrial food webs, which may experience cascading effects derived from changes in primary production through so-called &amp;#8220;bottom-up&amp;#8221; effects. We synthesize current knowledge on i) the changing Arctic marine icescape, ii) the drivers of biological changes for Arctic marine primary production, iii) the different pulses of Arctic marine primary production, iv) patterns of marine trophic and phenological changes, and iv) some mechanisms through which sea-ice dynamics ostensibly influence terrestrial primary productivity. We deliver a set of predictions for key productivity indicators, propose a semi-quantitative model of the expected future changes in primary production in the ice-covered Arctic Ocean, and close with an overview of the challenges ahead for reaching a holistic and comprehensive understanding of the ecosystem dynamical consequences and associated impacts on human life of warming-related sea-ice decline.&lt;/p&gt;

An algal enzyme required for biosynthesis of the most abundant marine carotenoids

Fucoxanthin and its derivatives are the main light-harvesting pigments in the photosynthetic apparatus of many chromalveolate algae and represent the most abundant carotenoids in the world’s oceans, thus being major facilitators of marine primary production. A central step in fucoxanthin biosynthesis that has been elusive so far is the conversion of violaxanthin to neoxanthin. Here, we show that in chromalveolates, this reaction is catalyzed by violaxanthin de-epoxidase–like (VDL) proteins and that VDL is also involved in the formation of other light-harvesting carotenoids such as peridinin or vaucheriaxanthin. VDL is closely related to the photoprotective enzyme violaxanthin de-epoxidase that operates in plants and most algae, revealing that in major phyla of marine algae, an ancient gene duplication triggered the evolution of carotenoid functions beyond photoprotection toward light harvesting.

Silicified cell walls as a defensive trait in diatoms

Diatoms contribute nearly half of the marine primary production. These microalgae differ from other phytoplankton groups in having a silicified cell wall, which is the strongest known biological material relative to its density. While it has been suggested that a siliceous wall may have evolved as a mechanical protection against grazing, empirical evidence of its defensive role is limited. Here, we experimentally demonstrate that grazing by adult copepods and nauplii on diatoms is approximately inversely proportional to their silica content, both within and among diatom species. While a sixfold increase in silica content leads to a fourfold decrease in copepod grazing, silicification provides no protection against protozoan grazers that directly engulf their prey. We also found that the wall provides limited protection to cells ingested by copepods, since less than 1% of consumed cells were alive in the faecal pellets. Moreover, silica deposition in diatoms decreases with increasing growth rates, suggesting a possible cost of defence. Overall, our results demonstrate that thickening of silica walls is an effective defence strategy against copepods. This suggests that the plasticity of silicification in diatoms may have evolved as a response to copepod grazing pressure, whose specialized tools to break silicified walls have coevolved with diatoms.