Neutrophils Promote Larynx Squamous Cell Carcinoma Progression via Activating the IL-17/JAK/STAT3 Pathway

Laryngeal squamous cell carcinoma (LSCC) is the main type of laryngeal cancer with poor prognosis. Incidence of LSCC increases every year, posing a great threat to human health. The underlying mechanism needs further study. Neutrophils are the most prevalent type of immune cells, which play vital roles in crosstalk between the microenvironment and cancer cells. In our study, we aim to figure out the complex regulation between neutrophils and LSCC. Our experiments showed that LSCC cells could promote the activation and mobility of neutrophils. And, in return, neutrophils enhanced the proliferation, migration, and invasion of LSCC. The subsequent results showed that IL-17 was highly expressed in neutrophil conditioned medium. Block of IL-17 could effectively inhibit the progression of LSCC induced by neutrophils. What is more, the results showed that IL-17 activated the JAK/STAT3 pathway in LSCC. Inhibition of the JAK/STAT3 pathway could significantly block neutrophil-induced LSCC progression. Our research reveals the complex interaction between neutrophils and LSCC cells, providing new ideas for the treatment of LSCC.

Results of conservative treatment of locally advanced larynx squamous cell carcinoma using intraarterial regional polychemotherapy

The study objective is to evaluate the results of organ-preserving treatment of locally advanced larynx squamous cell carcinoma.Materials and methods. Analysis of 28 patients with locally advanced larynx squamous cell carcinoma (stages III-IV) who underwent treatment at the N.N. Blokhin National Medical Research Center of Oncology of the Ministry of Health of Russia between 2017 and 2020 was performed. At the first stage, 2-3 courses of combined inductive polychemotherapy per the DCF scheme with 21-day interval were performed (docetaxel 60 mg/m2, cisplatin 60 mg/m2 intraarterially bolus with detoxication with sodium thiosulfate, 5-fluorouracil 1000 mg/m2/day as 96-hour infusion). The second stage included external beam radiotherapy (60-70 Gy, 2 Gy per day 5 days a week) as monotreatment if complete clinical response after chemotherapy was achieved or with regional administration of cisplatin (60 mg/m2 once per 3 weeks) if after inductive stage full clinical response wasn»t observed.Results. In 20 (71.5 %) patients, complete clinical response was observed after inductive treatment; in 7 (25 %) patients, partial response was observed. Tumor stabilization was detected in 1 (3.5 %) patient. Two-year overall survival was 95.8 ± 4.1 %, progression-free survival was 90.1 ± 6.8 %.Conclusion. The proposed strategy of organ-preserving treatment of stage III-IV larynx cancer with substitution of systemic chemotherapy with regional intraarterial chemotherapy prior to radiotherapy is highly effective from the point of view of direct anti-tumor effect and recurrence-free and overall survival.

HPV, p16 and p53 As Indicators of Response to Survival in Larynx Squamous Cell Carcinoma

Purpose: To analyze the relationship between the prognosis of patients with larynx squamous cell carcinoma (LSCC) and human papillomavirus (HPV) infection, p16 and p53 protein expression. Methods: All patients were treated at the department of radiation oncology, Anhui provincial hospital, between May 2005 and May 2012. The 41 consecutive patients with LSCC were treated surgically and received postoperative radiotherapy. Analyses of pathology specimen were surgically removed and performed on formalin-fixed, paraffin-embedded tissue samples. HPV DNA sequences in tumor tissues were screened by a commercial Luminex technique for HPVs and HPV-specific PCR assays. P16 and p53 protein expression were detected by immunohistochemical staining. Overall survival(OS)and progression-free survival(PFS)for HPV-positive and HPV-negative patients, p16-positive and p16-negative patients, p53-positive and p53-negative patients were estimated by Kaplan-Meier analysis. Cox regression model was used for multivariate analysis. Results: HPV-DNA was detected in 4(9.7%)of all specimens. Among them, 3 were positive for HPV-56,1 for HPV-16. With the follow-up of 3-78 months(a median of 34 months),patients with HPV-positive tumors had better overall survival than patients with HPV-positive tumors(75% vs 61%, P>0.05). Multivariate analysis by Cox regression model showed that nodal status was independent prognostic factors for patients with LSCC(P<0.05). Conclusions: HPV status is not an independent prognostic factor. Nodal status was independent prognostic factors for patients with LSCC.

Incidence of Occult Lymph Node Metastasis in Primary Larynx Squamous Cell Carcinoma, by Subsite, T Classification and Neck Level: A Systematic Review

Background: Larynx cancer is a common site for tumors of the upper aerodigestive tract. In cases with a clinically negative neck, the indications for an elective neck treatment are still debated. The objective is to define the prevalence of occult metastasis based on the subsite of the primary tumor, T classification and neck node levels involved. Methods: All studies included provided the rate of occult metastases in cN0 larynx squamous cell carcinoma patients. The main outcome was the incidence of occult metastasis. The pooled incidence was calculated with random effects analysis. Results: 36 studies with 3803 patients fulfilled the criteria. The incidence of lymph node metastases for supraglottic and glottic tumors was 19.9% (95% CI 16.4–23.4) and 8.0% (95% CI 2.7–13.3), respectively. The incidence of occult metastasis for level I, level IV and level V was 2.4% (95% CI 0–6.1%), 2.0% (95% CI 0.9–3.1) and 0.4% (95% CI 0–1.0%), respectively. For all tumors, the incidence for sublevel IIB was 0.5% (95% CI 0–1.3). Conclusions: The incidence of occult lymph node metastasis is higher in supraglottic and T3–4 tumors. Level I and V and sublevel IIB should not be routinely included in the elective neck treatment of cN0 laryngeal cancer and, in addition, level IV should not be routinely included in cases of supraglottic tumors.

Diagnosed laryngeal cancer relapse using harpoon biopsy under ultrasound guidance (clinical case)

The objective is to demonstrate possibilities of a harpoon biopsy under ultrasound guidance for malignant neoplasms early detection using a clinical example.Clinical case. A 65-year-old patient with morphologically verified laryngeal cancer (stage II, T2N0M0) underwent radiation therapy, which resulted in a complete tumor resorption. Subsequent ultrasound detected a neoplasm in the projection of the folding section. Percutaneous harpoon biopsy was performed under ultrasound guidance because standard clinical and instrumental studies were unable to confirm the diagnosis. Histological study of the biopsy material confirmed the “relapse of larynx squamous cell carcinoma”.Conclusion. A transdermal harpoon biopsy under ultrasound guidance followed by morphological biopsy study makes it possible to diagnose the disease and determine therapeutic approach as soon as possible.