Evaluating Cross-Resistance to Cry and Vip Toxins in Four Strains of Helicoverpa armigera With Different Genetic Mechanisms of Resistance to Bt Toxin Cry1Ac

Evolution of resistance by pests has diminished the efficacy of transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt). In China, where transgenic cotton producing Bt toxin Cry1Ac has been planted since 1997, field control failures have not been reported but the frequency of resistance to Cry1Ac has increased in the cotton bollworm, Helicoverpa armigera. This provides incentive to switch to multi-toxin Bt cotton, which is grown in many other countries. Previous work created four laboratory strains of H. armigera with >100-fold resistance to Cry1Ac, with the genetic basis of resistance known in all but the LF256 strain. Here, we analyzed the genetic basis of resistance in Cry1Ac in LF256 and evaluated cross-resistance of all four strains to three toxins produced by widely planted multi-toxin Bt cotton: Cry1Fa, Cry2Ab, and Vip3Aa. DNA sequencing revealed that LF256 lacked the mutations in three genes (HaTSPAN1, HaABCC2, and HaABCC3) that confer resistance to Cry1Ac in two other strains of H. armigera we analyzed. Together with previous results, the data reported here show that each of the four strains examined has a different genetic basis of resistance to Cry1Ac. Significant positive cross-resistance occurred to Cry1Fa in three of the four strains tested but not to Cry2Ab or Vip3Aa in any strain. Thus, Cry2Ab and Vip3Aa are likely to be especially valuable for increasing the efficacy and durability of Bt cotton against H. armigera populations that have some resistance to Cry1Ac.

Self-control Failure Increases a Strategic Preference for Submission as Means to Avoid Future Failure

A plethora of theories on human motives proposes that people have a fundamental need for control and an intrinsic desire to avoid submission to others. The current paper investigated an important exception to this general claim. Five experiments show that self-control failure leads people to strategically prioritize more social submission. In Experiments 1 to 3, salience of self-control failure increased the preference for submission. The submission effect was replicated with two manipulations and four measures of submission. Additionally, Experiment 3 showed that the effect only occurs after self-control failure and not after failure in controlling others. Finally, in Experiments 4 and 5, the submission effect influenced concrete preferences related to actual self-control failures from participants’ personal lives. When confronted with a high likelihood of self-control failure (versus moderate likelihood), participants preferred more an intervention program offering external control rather than an intervention program offering guidance (Experiment 4) or pre-commitment (Experiment 5). Together, these findings show a highly replicable effect whereby strategical considerations prompt people to invite domination and seek submission to others.

Low Self-Control: A Hidden Cause of Loneliness?

Loneliness has been associated with multiple negative outcomes. But what contributes to loneliness in the first place? Drawing from the literature on the importance of self-regulatory ability for successful social functioning, the present research explored the role of low self-control as a factor leading to loneliness. A set of four studies (and three additional studies in Supplementary Online Materials ) using cross-sectional, experimental, daily diary, and experience sampling methods showed that lower self-control is associated with higher loneliness at both trait and state levels. Why does low self-control contribute to loneliness? Self-control failures that have negative implications for others lead to higher risks for being ostracized by others, which predicts increased feelings of loneliness over time. These results suggest that low self-control, which is often associated with negative intrapersonal outcomes, can have important interpersonal consequences by evoking ostracism, and consequently, loneliness.

Conceptualizing Self-control on Problematic Social Media Use

With increasing concerns about problematic social media use, self-control is expected to become an effective approach for excessive users to decrease possible harm for their well-being. This article explores the current literature on the conceptualization of self-control on social media. For this, 25 papers from seven academic databases were analyzed in the chronological order in a systematic literature review. The sequence of applied frameworks demonstrates a gradual switch from theories of planned behavior to theories justifying non-planned behavior and self-control failures. This finding explains the emphasis of recent studies on the impulsive behavior of excessive social media users and the application of dual-system theories. However, research design of selected articles included mainly self-report tools to investigate impulsive self-control failures which may result in contradictory findings and deficient theoretical grounding for self-control interventions. All investigated papers claim a negative impact of social media self-control failures on personal well-being.

Estimating the Failure Risk of Hotel-based Quarantine for Preventing COVID-19 Outbreaks in Australia and New Zealand

AimWith increasing global use of hotel-based quarantine as part of COVID-19 border control efforts, we aimed to assess its risk of failure.MethodsWe searched official websites in both Australia and New Zealand (NZ) to identify outbreaks and border control failures associated with hotel quarantine (searches conducted up to 12 February 2021). We used two denominators: a) the estimated number of travelers who went through these facilities during the 2020 year up to 31 January 2021; and b) the equivalent number of SARS-CoV-2 positive people who went through these facilities.ResultsUp to 31 January 2021, Australia had seven failures with one causing over 800 deaths and six resulting in lockdowns. In NZ there were nine failures, with one causing an outbreak with three deaths, and also a lockdown. The overall failure risk for those transiting quarantine was estimated at one failure per 20,702 travelers and one failure per 252 SARS-CoV-2 positive cases (both countries combined). At the country level, there were 15.5 failures per 1000 SARS-CoV-2 positive cases transiting quarantine in NZ (95%CI: 5.4 to 25.7), compared to 2.0 per 1000 SARS-CoV-2 positive cases in Australia (0.5 to 3.5) – a greater than seven-fold difference in risk. Approaches to infection control and surveillance in hotel quarantine were found to vary widely by country and by state/territory.ConclusionsThere appears to be a notable risk of failure with the use of hotel quarantine in these two countries. The large variation in infection control practices suggests opportunity for risk reduction.

Auditor Evaluation of Manager's Competence After a Failure in Internal Control

Audit guidance requires auditors to assess management's competence with respect to internal controls over financial reporting (ICFR) based on the recommendations of COSO's integrated framework. The omission bias theory suggests that after internal control failures, auditors may assess managers' competence in a manner that is inconsistent with the requirements of the audit guidance. Results from four experiments using 313 experienced audit and accounting professionals support this concern and a means of mitigating it. I find that auditors are vulnerable to the omission bias, viewing the manager to be least responsible for the control failure and, therefore, most competent when prior to the failure the manager did nothing to prevent it. I also find that auditors can reduce their vulnerability to this bias by forewarning their clients about the increased importance of a key control in an area of potential future concern-a non-required practice encouraged by the audit guidance. Consistent with omission bias literature, I find that forewarning makes omission of action unexpected and thereby mitigates the omission bias. My results also confirm that auditors incorporate competence judgments into their evaluations of the ICFR, as required by the audit guidance.

The Error-Related Negativity Predicts Self-Control Failures in Daily Life

Adaptive behavior critically depends on performance monitoring (PM), the ability to monitor action outcomes and the need to adapt behavior. PM-related brain activity has been linked to guiding decisions about whether action adaptation is warranted. The present study examined whether PM-related brain activity in a flanker task, as measured by electroencephalography (EEG), was associated with adaptive behavior in daily life. Specifically, we were interested in the employment of self-control, operationalized as self-control failures (SCFs), and measured using ecological momentary assessment. Analyses were conducted using an adaptive elastic net regression to predict SCFs from EEG in a sample of 131 participants. The model was fit using within-subject averaged response-locked EEG activity at each electrode and time point within an epoch surrounding the response. We found that higher amplitudes of the error-related negativity (ERN) were related to fewer SCFs. This suggests that lower error-related activity may relate to lower recruitment of interventive self-control in daily life. Altered cognitive control processes, like PM, have been proposed as underlying mechanisms for various mental disorders. Understanding how alterations in PM relate to regulatory control might therefore aid in delineating how these alterations contribute to different psychopathologies.

Fungicide Resistance Profiles of Botrytis cinerea Isolates From Michigan Vineyards and Development of a TaqMan Assay for Detection of Fenhexamid Resistance

Botrytis cinerea on grapes causes bunch rot at both pre- and postharvest stages, in which losses can reach up to 100%. Chemical control primarily relies on the prophylactic use of site-specific fungicides. Repeated applications of these products raise the risk of fungicide resistance development in B. cinerea populations, which can result in disease control failures. To determine the extent of resistance, B. cinerea isolates were collected from grape clusters in the northwest and southwest grape growing regions of Michigan in 2014 and 2018 (n = 115 and 125, respectively). These isolates were phenotyped using discriminatory doses of eight fungicides to determine the levels of resistance. Fungicide resistance increased from 2014 to 2018, mostly affecting the active ingredients fenhexamid, fluopyram, and iprodione. B. cinerea isolates resistant to multiple fungicides were detected in 2014 and 2018, with a higher frequency of resistance in 2018. TaqMan real-time PCR has been developed to detect B. cinerea fungicide resistance to fenhexamid and to differentiate the erg27 F412S/I/V alleles. The TaqMan assay was tested for sensitivity, specificity, and reproducibility on purified DNA and infected grape tissue samples. Our data provide essential information to growers about the efficacy for B. cinerea control using the available botryticides. Furthermore, the developed fenhexamid markers will be transferred to diagnostic clinics to assist growers in the management of bunch rot before resistance-related control failures occur.