Invasive slipper limpets Crepidula fornicata act like a sink, rather than source, of Vibrio spp.

A large knowledge gap exists regarding the disease profile and pathologic condition of the invasive, non-native, slipper limpet Crepidula fornicata. To help address this, we performed a yearlong health survey across two sites in South Wales UK, subtidal Swansea Bay and intertidal Milford Haven. In total, 1,800 limpets were screened systematically for haemolymph bacterial burdens using both general and vibrio-selective growth media (TSA +2% NaCl and TCBS, respectively), haemolymph (blood) inspection using microscopy, a PCR-based assay targeting Vibrio spp., and multi-tissue histology. Over 99% of haemolymph samples contained cultivable bacterial colony forming units, and 83% of limpets tested positive for the presence of vibrios via PCR (confirmed via Sanger sequencing). Vibrio presence did not vary greatly across sites, yet a strong temporal (seasonal) effect was observed - significantly higher bacterial loads during the summer. Binomial logistic regression models revealed larger (older) limpets were more likely to harbour vibrios, and the growth of bacteria on TCBS was a key predictor for PCR-based vibrio detection. Histological assessment of >340 animals revealed little evidence of inflammation, sepsis, or immune reactivity despite the gross bacterial numbers. We contend that slipper limpets are not susceptible to bacteriosis at either site surveyed, or do not to harbour vibrios known to be pathogenic to humans. The lack of susceptibility to local pathogenic bacteria may explain, in part, the invasion success of C. fornicata across this region.

Reflections on lymphoedema deployment into community services during the pandemic

During the second wave of the COVID-19 pandemic, district nursing teams were overwhelmed with their caseload due to the palliative care needs of their patients. This led to patients with wet legs and chronic wounds deteriorating due to staffing levels. Therefore, the Swansea Bay University Health Board and Lymphoedema Network Wales teams redeployed two working time equivalents (WTE) into the community to take over the management of these patients with chronic wounds for 4 months. The clinicians came from a variety of different backgrounds, including nursing, physiotherapy, emergency medicine and occupational therapy. Between the teams, 866 visits were carried out over the 4-month period, where patients' compression therapy was altered to promote healing and reduce oedema. At the end of the 4-month period, 21% of the patients were discharged off the district nursing caseload completely, while of the 60% who were still active caseload patients, 35% were in increased compression and 20% had reduced need for visits.

1098 Prophylactic Doxycycline Prior To The Excision of Ulcerated Skin Lesions. An Audit of Dose Timings and Adverse Effects

Aim Antibiotic prophylaxis to reduce the incidence of surgical site infections following excision of ulcerated skin lesions is frequently recommended, although the evidence base is limited. Current Swansea Bay guidelines recommend pre-operative administration of antibiotics: for procedures performed under local anaesthetic in the Plastic Surgery Treatment Centre (PSTC), oral route is preferable. It was noted that a number of patients were experiencing nausea and vomiting following administration of the recommended oral agent (doxycycline). The aim of this audit was to quantify the proportion of patients experiencing adverse effects, and to assess whether the prophylactic dose was being administered within the recommended time frame. Method Data was collected over a three-week period (28/10/20- 18/11/20). All patients that received doxycycline pre-operatively during this period were included: the time the doxycycline was given, the time of operation and whether there were any adverse effects was recorded for each patient. Results 14 patients received doxycycline during the monitored period. On average the doxycycline was given 52 minutes prior to the procedure. 2 patients received their doxycycline dose after their procedures. 4 patients experienced side effects from the doxycycline (3 vomited, 1 nausea), the average time this subset of patients received their dose was 50 minutes prior to their procedure. Conclusions A significant proportion of patients experience adverse after receiving doxycycline, whilst there is limited evidence to prove its benefits. Trust guidelines are under review to consider alternative antibiotics, but better evidence is required to clarify the need for antibiotic prophylaxis for ulcerated lesions.

City region limits: Questioning city-centric growth narratives in medium-sized cities

This City Deal will provide the region and its partners with the new ways of working and resources to unlock significant economic growth across the Swansea Bay City Region. It is a Deal where both Welsh and UK Governments have committed to jointly invest, subject to the submission and approval of full business cases in relation to the 11 identified projects and the agreement of governance arrangements for the deal, up to £241 million on specific interventions which seek to support and further build on the region’s strengths which include health, energy and manufacturing sectors and are underpinned by a world-class digital infrastructure, successful universities and innovative health boards.

The influence of bait on remote underwater video observations in shallow-water coastal environments associated with the North-Eastern Atlantic

The use of baited remote underwater video (BRUV) for examining and monitoring marine biodiversity in temperate marine environments is rapidly growing, however many aspects of their effectiveness relies on assumptions based on studies from the Southern Hemisphere. The addition of bait to underwater camera systems acts as a stimulus for attracting individuals towards the camera field of view, however knowledge of the effectiveness of different bait types in northern temperate climbs is limited, particularly in dynamic coastal environments. Studies in the Southern Hemisphere indicate that oily baits are most effective whilst bait volume and weight do not impact BRUV effectiveness to any great degree. The present study assesses the influence of four bait types (mackerel, squid, crab and no bait (control)) on the relative abundance, taxonomic diversity and faunal assemblage composition at two independent locations within the North-Eastern Atlantic region; Swansea Bay, UK and Ria Formosa Lagoon, Portugal. Two different bait quantities (50 g and 350 g) were further trialled in Swansea Bay. Overall, patterns showed that baited deployments recorded statistically higher values of relative abundance and taxonomic diversity when compared to un-baited deployments in Swansea Bay but not in Ria Formosa Lagoon. No statistical evidence singled out one bait type as best performing for attracting higher abundances and taxonomic diversity in both locations. Faunal assemblage composition was however found to differ with bait type in Swansea Bay, with mackerel and squid attracting higher abundances of scavenging species compared to the crab and control treatments. With the exception of squid, bait quantity had minimal influence on bait attractiveness. It is recommended for consistency that a minimum of 50 g of cheap, oily fish such as mackerel is used as bait for BRUV deployments in shallow dynamic coastal environments in the North-Eastern Atlantic Region.

Diagnosis and prevalence of two new species of haplosporidians infecting shore crabs Carcinus maenas: Haplosporidium carcini n. sp., and H. cranc n. sp.

This study provides a morphological and phylogenetic characterization of two novel species of the order Haplosporida (Haplosporidium carcini n. sp., and H. cranc n. sp.) infecting the common shore crab Carcinus maenas collected at one location in Swansea Bay, South Wales, UK. Both parasites were observed in the haemolymph, gills and hepatopancreas. The prevalence of clinical infections (i.e. parasites seen directly in fresh haemolymph preparations) was low, at ~1%, whereas subclinical levels, detected by polymerase chain reaction, were slightly higher at ~2%. Although no spores were found in any of the infected crabs examined histologically (n = 334), the morphology of monokaryotic and dikaryotic unicellular stages of the parasites enabled differentiation between the two new species. Phylogenetic analyses of the new species based on the small subunit (SSU) rDNA gene placed H. cranc in a clade of otherwise uncharacterized environmental sequences from marine samples, and H. carcini in a clade with other crustacean-associated lineages.

Mycosis Is a Disease State Encountered Rarely in Shore Crabs, Carcinus maenas

There is a paucity of knowledge regarding the diversity and impact(s) of disease-causing fungi in marine animals, especially shellfish. In efforts to address this knowledge gap for the shore crab Carcinus maenas, a year-long disease screen was carried out across two sites in Swansea Bay (Wales, UK) with a view to characterising putative fungal infections. Crabs were sampled between November 2017 and October 2018, and screened systematically for disease signatures using haemolymph (blood) preparations, targeted PCR and tissue histopathology. Strikingly, mycosis was confirmed in ~0.4% of total crabs tested (n = 1191) and restricted to one location only (Mumbles Pier). Clinical infections were observed in four out of four infected crabs. In these animals, the gills and hepatopancreas were congested with fungal morphotypes. In addition, some evidence indicates haemocyte (immune cell) reactivity toward the fungi. Phylogenetic placement of the partial internal transcribed spacer (ITS1) gene regions amplified from three mycotic crabs revealed the causative agent to be related to hypocrealean fungi, thereby representing a novel species.

Longshore variation in coastal foredune growth on a megatidal beach from UAV measurements

<p>Coastal dunes are both a vital natural coastal defence and a key ecological habitat; therefore, understanding their evolution is important to inform coastal management. Megatidal environments are the world largest tidal ranges and hence provide a unique endmember of the tidal range continuum. A study site at Crymlyn Burrows, Swansea Bay, UK is monitored here; the area was originally of applied interest due to its identification as a key receptor of the Swansea Bay Tidal Lagoon project. The study site comprises of 2km of dune frontage bounded to the west by hard sea defences and to the east by the River Neath estuary. The intertidal is characterized by a shallow slope and crescentic intertidal bars. Mean spring tidal range at the nearby Mumbles tide gauge is 8.46m; mean wave heights at a wave buoy offshore of the site (depth 9m LAT) are 0.66m and storm wave heights exceed 3m; predominant wind direction is in an alongshore – onshore direction.</p><p>A Sensefly Ebee-RTK drone with a Sony RGB camera has been used to map the dune system and the mid to upper intertidal beach on a monthly – bimonthly frequency since October 2018. Initial post-processing was conducted in the Sensefly Emotion3 software; Pix4D was then used to generate a point cloud from the georeferenced images. RTK-GPS surveyed ground control points distributed over the study area were used to improve the accuracy of the solution. Point clouds were cleaned to remove noise using Cloud Compare, an open source point cloud editor, before being interpolated onto a gridded surface. Comparison of the gridded surface against RTK-GPS surveyed points gave a vertical mean absolute error (MAE) of 0.05m over the beach area. Comparison in the dune area is more complex since the raw point cloud includes the vegetation and hence over-estimates height compared to the bare earth. Based on the raw point cloud, MAE over the dune area was 0.22m; however, when vegetation points were removed using artificial neural network based colour discrimination, the MAE was 0.05m.</p><p>Longshore variation in dune evolution is clearly evident. At the eastern and western ends of the dune system, dune progradation can be observed whereas in the central portion the frontal dune is cliffed and the dune foot position is static or eroding landward. Pressure transducers have been deployed in a longshore array at the neap high tide level to assess variation in wave energy reaching the upper intertidal over the study area.</p><p>This presentation will explore whether this variation in behavior is due to longshore variation in wave energy (erosion potential), variation in sediment availability (accretion potential) or the persistence of antecedent morphology.</p>

Spatial and temporal disease dynamics of the parasite Hematodinium sp. in shore crabs, Carcinus maenas

Background The parasitic dinoflagellates of the genus Hematodinium represent the causative agent of so-called bitter or pink crab disease in a broad range of shellfish taxa. Outbreaks of Hematodinium-associated disease can devastate local fishing and aquaculture efforts. The goal of our study was to examine the potential role of the common shore (green) crab Carcinus maenas as a reservoir for Hematodinium. Carcinus maenas is native to all shores of the UK and Ireland and the North East Atlantic but has been introduced to, and subsequently invaded waters of, the USA, South Africa and Australia. This species is notable for its capacity to harbour a range of micro- and macro-parasites, and therefore may act as a vector for disease transfer. Methods Over a 12-month period, we interrogated 1191 crabs across two distinct locations (intertidal pier, semi-closed dock) in Swansea Bay (Wales, UK) for the presence and severity of Hematodinium in the haemolymph, gills, hepatopancreas and surrounding waters (eDNA) using PCR-based methods, haemolymph preparations and histopathology. Results Overall, 13.6% were Hematodinium-positive via PCR and confirmed via tissue examination. Only a small difference was observed between locations with 14.4% and 12.8% infected crabs in the Dock and Pier, respectively. Binomial logistic regression models revealed seasonality (P < 0.002) and sex (P < 0.001) to be significant factors in Hematodinium detection with peak infection recorded in spring (March to May). Male crabs overall were more likely to be infected. Phylogenetic analyses of the partial ITS and 18S rRNA gene regions of Hematodinium amplified from crabs determined the causative agent to be the host generalist Hematodinium sp., which blights several valuable crustaceans in the UK alone, including edible crabs (Cancer pagurus) and langoustines (Nephrops norvegicus). Conclusions Shore crabs were infected with the host generalist parasite Hematodinium sp. in each location tested, thereby enabling the parasite to persist in an environment shared with commercially important shellfish.

Implementation of a Full Momentum Conservative Approach in Modelling Flow Through Tidal Structures

Understanding the impact of various hydraulic structures, such as coastal reservoirs and tidal range impoundments, has been one of the key challenges of hydro–environmental engineering in recent years. Over the last half-century, several proposals for tidal range schemes in the UK have been scrutinised and then abandoned due to the uncertainty over the environmental footprint and/or the cost of electricity. Therefore, it is essential to understand the fundamental assumptions for reliable hydrodynamic analysis of these projects. This study examined the impact of the fully conserved momentum through tidal structures using a novel approach. The method was applied to 2D and 3D versions of the regional model of Swansea Bay tidal lagoon, examining two different types of velocity distribution at turbine exit. A simplified distribution significantly increased the velocity and length of the jet exiting the turbines during power generation. A realistic distribution gave more accurate results, with jet velocities more closely resembling the situation without including the momentum. The 3D model with realistic distribution has markedly improved the resulting vertical velocity profile. The value of the improved methodology for momentum conservation has proved to be particularly useful in local-scale studies. It can be applied to other similar hydraulic structures and used for the analysis of sediment transport, water quality, etc.