Strategies of desiccation tolerance vary across life phases in the moss Syntrichia caninervis

Ultraviolet radiation (UVR) is a major environmental stressor for terrestrial plants. Here we investigated genetic responses to acute broadband UVR exposure in the highly desiccation-tolerant mosses Syntrichia caninervis and Syntrichia ruralis, using a comparative transcriptomics approach. We explored whether UVR protection is physiologically plastic and induced by UVR exposure, addressing the following questions: (1) What is the timeline of changes in the transcriptome with acute UVR exposure in these two species? (2) What genes are involved in the UVR response? and (3) How do the two species differ in their transcriptomic response to UVR? There were remarkable differences between the two species after 10 and 30 min of UVR exposure, including no overlap in significantly differentially abundant transcripts (DATs) after 10 min of UVR exposure and more than twice as many DATs for S. caninervis as there were for S. ruralis. Photosynthesis-related transcripts were involved in the response of S. ruralis to UVR, while membrane-related transcripts were indicated in the response of S. caninervis. In both species, transcripts involved in oxidative stress and those important for desiccation tolerance (such as late embryogenesis abundant genes and early light-inducible protein genes) were involved in response to UVR, suggesting possible roles in UVR tolerance and cross-talk with desiccation tolerance in these species. The results of this study suggest potential UVR-induced responses that may have roles outside of UVR tolerance, and that the response to URV is different in these two species, perhaps a reflection of adaptation to different environmental conditions.

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Propagation of desert moss Syntrichia caninervis in peat pellet: a method for rapidly obtaining large numbers of cloned gametophytes

10.21203/rs.3.rs-143310/v1 ◽

2021 ◽

Author(s):

Xiujin Liu ◽

Ping Zhou ◽

Xiaoshuang Li ◽

Daoyuan Zhang

Keyword(s):

Desiccation Tolerance ◽

Current Knowledge ◽

Biological Soil Crust ◽

Body Part ◽

Regeneration Potential ◽

Dominant Type ◽

Syntrichia Caninervis ◽

Large Numbers ◽

Ability To Regenerate ◽

Different Parts

Abstract Background Syntrichia caninervis is a typical desiccation tolerance moss, which is a dominant species forming biological soil crust in the Gurbantunggut Desert. This study investigated the effect of different explants on regeneration potential by propagating on peat pellet. Result Juvenile and green leaves can regenerate secondary protonema within one week and shoot in half a month in peat pellet. Rhizoid has strong ability to regenerate, like leaf regeneration, the secondary protonema is the dominant type of regenerant. The process of stem regeneration is similar with whole gametophyte. Stem is the most important integral body part when propagation. The whole gametophyte is the most optimal materials for rapidly propagating. Conclusion This article improves the state of our current knowledge in desiccation tolerance moss cultivation, highlighting efforts to effectively obtain large number of gametophytes through different parts of explant. This work provides a useful resource for the research of S. caninervis as well as biocrust restoration.

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Respiratory pathways in germinating maize radicles correlated with desiccation tolerance and soluble sugars

Physiologia Plantarum ◽

10.1034/j.1399-3054.1992.850404.x ◽

1992 ◽

Vol 85 (4) ◽

pp. 581-588 ◽

Cited By ~ 3

Author(s):

Olivier Leprince ◽

Adrie van der Werf ◽

Roger Deltour ◽

Hans Lambers

Keyword(s):

Desiccation Tolerance ◽

Soluble Sugars

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Acquisition of desiccation tolerance in faba bean (Vicia Faba) as affected by maturity stages, harvested organ type, and drying method

Pakistan Journal of Botany ◽

10.30848/pjb2019-1(6) ◽

2018 ◽

Vol 51 (1) ◽

Author(s):

Abdul Majeed Baloch ◽

Tongtong Zhai ◽

Abdul Wahid Baloch ◽

Zhihua Liu ◽

Xingtang Yang ◽

...

Keyword(s):

Vicia Faba ◽

Faba Bean ◽

Desiccation Tolerance ◽

Drying Method ◽

Maturity Stages ◽

Organ Type

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Changes in embryo drying rates, the alignment of lipid bodies along the plasma membrane, and dehydrin-like proteins during the acquisition of desiccation tolerance in maize seed

10.31274/rtd-180813-9858 ◽

2001 ◽

Author(s):

Leobigildo Cordova-Tellez

Keyword(s):

Plasma Membrane ◽

Desiccation Tolerance ◽

Lipid Bodies ◽

Maize Seed ◽

Drying Rates

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Common and Specific Mechanisms of Desiccation Tolerance in Two Gesneriaceae Resurrection Plants. Multiomics Evidences

Frontiers in Plant Science ◽

10.3389/fpls.2019.01067 ◽

2019 ◽

Vol 10 ◽

Cited By ~ 4

Author(s):

Jie Liu ◽

Daniela Moyankova ◽

Dimitar Djilianov ◽

Xin Deng

Keyword(s):

Desiccation Tolerance ◽

Resurrection Plants

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Desiccation Tolerance: Avoiding Cellular Damage During Drying and Rehydration

Annual Review of Plant Biology ◽

10.1146/annurev-arplant-071219-105542 ◽

2020 ◽

Vol 71 (1) ◽

pp. 435-460 ◽

Cited By ~ 11

Author(s):

Melvin J. Oliver ◽

Jill M. Farrant ◽

Henk W.M. Hilhorst ◽

Sagadevan Mundree ◽

Brett Williams ◽

...

Keyword(s):

Desiccation Tolerance ◽

Cell Damage ◽

Cellular Responses ◽

Land Plants ◽

Cellular Damage ◽

Cellular Protection ◽

Vegetative Tissues ◽

Tolerant Plants ◽

Core Set ◽

Protection Mechanisms

Desiccation of plants is often lethal but is tolerated by the majority of seeds and by vegetative tissues of only a small number of land plants. Desiccation tolerance is an ancient trait, lost from vegetative tissues following the appearance of tracheids but reappearing in several lineages when selection pressures favored its evolution. Cells of all desiccation-tolerant plants and seeds must possess a core set of mechanisms to protect them from desiccation- and rehydration-induced damage. This review explores how desiccation generates cell damage and how tolerant cells assuage the complex array of mechanical, structural, metabolic, and chemical stresses and survive.Likewise, the stress of rehydration requires appropriate mitigating cellular responses. We also explore what comparative genomics, both structural and responsive, have added to our understanding of cellular protection mechanisms induced by desiccation, and how vegetative desiccation tolerance circumvents destructive, stress-induced cell senescence.

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Peptide-Bound Methionine Sulfoxide (MetO) Levels and MsrB2 Abundance Are Differentially Regulated during the Desiccation Phase in Contrasted Acer Seeds

Antioxidants ◽

10.3390/antiox9050391 ◽

2020 ◽

Vol 9 (5) ◽

pp. 391 ◽

Cited By ~ 3

Author(s):

Natalia Wojciechowska ◽

Shirin Alipour ◽

Ewelina Stolarska ◽

Karolina Bilska ◽

Pascal Rey ◽

...

Keyword(s):

Desiccation Tolerance ◽

Methionine Sulfoxide ◽

Redox Status ◽

Embryonic Axes ◽

Seed Desiccation ◽

Methionine Sulfoxide Reductases ◽

Norway Maple ◽

Orthodox Seeds ◽

Oxidation Of Methionine ◽

Reduced Forms

Norway maple and sycamore produce desiccation-tolerant (orthodox) and desiccation-sensitive (recalcitrant) seeds, respectively. Drying affects reduction and oxidation (redox) status in seeds. Oxidation of methionine to methionine sulfoxide (MetO) and reduction via methionine sulfoxide reductases (Msrs) have never been investigated in relation to seed desiccation tolerance. MetO levels and the abundance of Msrs were investigated in relation to levels of reactive oxygen species (ROS) such as hydrogen peroxide, superoxide anion radical and hydroxyl radical (•OH), and the levels of ascorbate and glutathione redox couples in gradually dried seeds. Peptide-bound MetO levels were positively correlated with ROS concentrations in the orthodox seeds. In particular, •OH affected MetO levels as well as the abundance of MsrB2 solely in the embryonic axes of Norway maple seeds. In this species, MsrB2 was present in oxidized and reduced forms, and the latter was favored by reduced glutathione and ascorbic acid. In contrast, sycamore seeds accumulated higher ROS levels. Additionally, MsrB2 was oxidized in sycamore throughout dehydration. In this context, the three elements •OH level, MetO content and MsrB2 abundance, linked together uniquely to Norway maple seeds, might be considered important players of the redox network associated with desiccation tolerance.

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Genome-Wide Role of HSF1 in Transcriptional Regulation of Desiccation Tolerance in the Anhydrobiotic Cell Line, Pv11

International Journal of Molecular Sciences ◽

10.3390/ijms22115798 ◽

2021 ◽

Vol 22 (11) ◽

pp. 5798

Author(s):

Shoko Tokumoto ◽

Yugo Miyata ◽

Ruslan Deviatiiarov ◽

Takahiro G. Yamada ◽

Yusuke Hiki ◽

...

Keyword(s):

Cell Line ◽

Cell Lines ◽

Desiccation Tolerance ◽

Expression Profiles ◽

Insect Cell Line ◽

Gene Expression Profiles ◽

Late Embryogenesis Abundant ◽

Heat Shock Factor 1 ◽

Genome Wide ◽

A Genome

The Pv11, an insect cell line established from the midge Polypedilum vanderplanki, is capable of extreme hypometabolic desiccation tolerance, so-called anhydrobiosis. We previously discovered that heat shock factor 1 (HSF1) contributes to the acquisition of desiccation tolerance by Pv11 cells, but the mechanistic details have yet to be elucidated. Here, by analyzing the gene expression profiles of newly established HSF1-knockout and -rescue cell lines, we show that HSF1 has a genome-wide effect on gene regulation in Pv11. The HSF1-knockout cells exhibit a reduced desiccation survival rate, but this is completely restored in HSF1-rescue cells. By comparing mRNA profiles of the two cell lines, we reveal that HSF1 induces anhydrobiosis-related genes, especially genes encoding late embryogenesis abundant proteins and thioredoxins, but represses a group of genes involved in basal cellular processes, thus promoting an extreme hypometabolism state in the cell. In addition, HSF1 binding motifs are enriched in the promoters of anhydrobiosis-related genes and we demonstrate binding of HSF1 to these promoters by ChIP-qPCR. Thus, HSF1 directly regulates the transcription of anhydrobiosis-related genes and consequently plays a pivotal role in the induction of anhydrobiotic ability in Pv11 cells.

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