scholarly journals Analysis of structure and gene expression in developing kidneys of male and female rats exposed to low protein diets in utero

2020 ◽  
Vol 303 (10) ◽  
pp. 2657-2667 ◽  
Author(s):  
Ryan J. Wood‐Bradley ◽  
Sarah L. Henry ◽  
Sanna Barrand ◽  
Anais Giot ◽  
Luke Eipper ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Utero ◽  
Female Rats ◽  
Male And Female ◽  
Low Protein ◽  
Male And Female Rats ◽  
Protein Diets

Pharmacology of lemongrass (Cymbopogon citratus Stapf). II. Effects of daily two month administration in male and female rats and in offspring exposed “in utero”

1986 ◽  
Vol 17 (1) ◽  
pp. 65-74 ◽  
Author(s):  
Maria Lucia O. Souza Formigoni ◽  
Helena Maria Lodder ◽  
Oswaldo Gianotti Filho ◽  
Tania M.S. Ferreira ◽  
E.A. Carlini
Keyword(s):  
In Utero ◽  
Female Rats ◽  
Cymbopogon Citratus ◽  
Male And Female ◽  
Male And Female Rats

scholarly journals Gene expression within the periaqueductal gray is linked to vocal behavior and early-onset parkinsonism in Pink1 knockout rats

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Cynthia A. Kelm-Nelson ◽  
Stephen Gammie
Keyword(s):  
Gene Expression ◽  
Early Onset ◽  
Early Stage ◽  
Protein Localization ◽  
Periaqueductal Gray ◽  
Female Rats ◽  
Vocal Behavior ◽  
Metabotropic Receptors ◽  
Male And Female ◽  
Male And Female Rats

Abstract Background Parkinson’s disease (PD) is a degenerative disease with early-stage pathology hypothesized to manifest in brainstem regions. Vocal deficits, including soft, monotone speech, result in significant clinical and quality of life issues and are present in 90% of PD patients; yet the underlying pathology mediating these significant voice deficits is unknown. The Pink1−/− rat is a valid model of early-onset PD that presents with analogous vocal communication deficits. Previous work shows abnormal α-synuclein protein aggregation in the periaqueductal gray (PAG), a brain region critical and necessary to the modulation of mammalian vocal behavior. In this study, we used high-throughput RNA sequencing to examine gene expression within the PAG of both male and female Pink1−/− rats as compared to age-matched wildtype controls. We used a bioinformatic approach to (1) test the hypothesis that loss of Pink1 in the PAG will influence the differential expression of genes that interact with Pink1, (2) highlight other key genes that relate to this type of Mendelian PD, and (3) catalog molecular targets that may be important for the production of rat vocalizations. Results Knockout of the Pink1 gene resulted in differentially expressed genes for both male and female rats that also mapped to human PD datasets. Pathway analysis highlighted several significant metabolic pathways. Weighted gene co-expression network analysis (WGCNA) was used to identify gene nodes and their interactions in (A) males, (B) females, and (C) combined-sexes datasets. For each analysis, within the module containing the Pink1 gene, Pink1 itself was the central node with the highest number of interactions with other genes including solute carriers, glutamate metabotropic receptors, and genes associated with protein localization. Strong connections between Pink1 and Krt2 and Hfe were found in both males and female datasets. In females a number of modules were significantly correlated with vocalization traits. Conclusions Overall, this work supports the premise that gene expression changes in the PAG may contribute to the vocal deficits observed in this PD rat model. Additionally, this dataset identifies genes that represent new therapeutic targets for PD voice disorders.

THE EFFECT OF PARATHION ON THE LIVER AND KIDNEY CARBOXYLESTERASES AND THE PLASMA ACETYLCHOLINESTERASES OF RATS FED NUTRITIONALLY DEFICIENT DIETS

1966 ◽  
Vol 44 (6) ◽  
pp. 809-817 ◽  
Author(s):  
Sheila I. Read ◽  
E. J. Middleton ◽  
W. P. Mckinley
Keyword(s):  
Control Diet ◽  
Acetylcholinesterase Activity ◽  
Protein Diet ◽  
Female Rats ◽  
Male Rats ◽  
Low Protein Diet ◽  
Male And Female ◽  
Low Protein ◽  
Male And Female Rats ◽  
Liver And Kidney

Female rats were fed diets low in minerals, vitamins, or protein, or a control diet, both alone and supplemented with 10 parts per million (p.p.m.) parathion for 3 weeks. Male and female rats were fed control and tow-vitamin diets both with and without parathion supplementation (0–10 p.p.m.) for 3 weeks. The liver and kidney carboxylesterases (EC 3.1.1.1.), and the plasma acetylcholinesterases (EC 3.1.1.7.) of the male rats, were measured.In the female rats, a low-mineral diet resulted in an increase of carboxylesterases in the liver and kidney; a low-vitamin diet caused a marked increase in liver carboxylesterases but had no effect on the carboxylesterases of the kidney. Parathion at 10 p.p.m. in all diets greatly reduced the liver carboxylesterases but had less effect on kidney carboxylesterases, except in the case of the low-protein diet, for which the reduction was similar to that in the liver. Varying amounts of parathion added to the low-vitamin diet reduced the liver and kidney carboxylesterases, but to a less extent than when added to the control diet.The liver carboxylesterases of male rats were inhibited approximately 50% by 2 p.p.m. parathion in the control diet and by 4 p.p.m. parathion in the low-vitamin diet. However, inhibition of plasma acetylcholinesterase and kidney carboxylesterases was not marked until the 10 p.p.m. parathion level was fed. The acetylcholinesterase activity of the plasma of male rats did not decrease until the level of liver carboxylesterases was very low.

Hyperplastic and Early Neoplastic Lesions of the Liver in Rats of Varying Ages with Dietary-Induced Cirrhosis

1969 ◽  
Vol 55 (2) ◽  
pp. 79-83 ◽  
Author(s):  
Melvin D. Reuber
Keyword(s):  
Female Rats ◽  
High Fat ◽  
Chemical Carcinogens ◽  
Deficient Diet ◽  
Male And Female ◽  
Low Protein ◽  
Male And Female Rats ◽  
Neoplastic Lesions ◽  
Parenchymal Cells ◽  
Age And Sex

Inbred Buffalo male and female rats, 4-, 8-, 12-, 24-, and 52-weeks old, ingested a high fat, low protein and choline deficient diet. Preneoplastic and early neoplastic lesions of the parenchymal cells, as well as cirrhosis, developed in the liver. Hyperplastic lesions generally were observed more often in male animals and in younger animals. The hyperplastic lesions, developing in the periportal regions, were similar histologically and with regard to age and sex to lesions demonstrated as precarcinogenic in animals given chemical carcinogens.

Chronic alcohol feeding and its influence on c-Fos and heat shock protein-70 gene expression in different brain regions of male and female rats

Metabolism ◽  
2002 ◽  
Vol 51 (12) ◽  
pp. 1562-1568 ◽  
Author(s):  
Tatsuo Nakahara ◽  
Makoto Hirano ◽  
Hideyuki Uchimura ◽  
Sima Shirali ◽  
Colin R. Martin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Brain Regions ◽  
Female Rats ◽  
Chronic Alcohol ◽  
Male And Female ◽  
Male And Female Rats

Mammary gland morphology and gene expression signature of weanling male and female rats following exposure to exogenous estradiol

2013 ◽  
Vol 238 (9) ◽  
pp. 1033-1046 ◽  
Author(s):  
Isabelle R Miousse ◽  
Horacio Gomez-Acevedo ◽  
Neha Sharma ◽  
Jamie Vantrease ◽  
Leah Hennings ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Gene Expression Signature ◽  
Female Rats ◽  
Male And Female ◽  
Expression Signature ◽  
Male And Female Rats ◽  
Mammary Gland Morphology

scholarly journals Effects of Diet-Induced Obesity on Hypothalamic Kisspeptin-Neurokinin-Dynorphin (KNDy) Neurons and Luteinizing Hormone Secretion in Sex Hormone-Primed Male and Female Rats

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. A537-A537
Author(s):  
Shiori Minabe ◽  
Kinuyo Iwata ◽  
Hitoshi Ozawa
Keyword(s):  
Gene Expression ◽  
Luteinizing Hormone ◽  
High Fat Diet ◽  
Female Rats ◽  
Male Rats ◽  
High Fat ◽  
Neurokinin B ◽  
Male And Female ◽  
Male And Female Rats ◽  
Kndy Neurons

Abstract Metabolic stress resulting from a nutrient excess causes infertility in both sexes. Kisspeptin-neurokinin B-dynorphin (KNDy) neurons in the arcuate nucleus (ARC) have been suggested to be key players in reproduction via direct stimulation of gonadotropin-releasing hormone (GnRH) and subsequent gonadotropin release in mammalian species. In this study, we investigated the sex differences in the effects of a high-fat diet (HFD) on KNDy-associated gene expression in the ARC to determine the pathogenic mechanism underlying obesity-induced infertility. Wistar-Imamichi strain male and female rats (7 weeks of age) were fed either a standard diet (10% calories from fat) or high-fat diet (45% calories from fat) for 4 months. In male rats, the HFD caused a significant suppression of Kiss1(encoding kisspeptin), Tac3(encoding neurokinin B), and Pdyn(encoding dynorphin A) gene expression in the ARC, resulting in a decrease in plasma luteinizing hormone (LH) levels. In female rats, 58% of the HFD-fed female rats exhibited irregular estrous cycles, while the other rats showed regular cycles. LH pulses were found, and the numbers of ARC Kiss1-,Tac3-, and Pdyn-expressing cells were high in control animals and almost allHFD-fed female rats, but two out of 10 rats showed profound HFD-induced suppression of LH pulse frequency and reduction in these cells. No statistical differences in LH secretion or ARC KNDy gene expression were observed between HFD-fed and control female rats. Additionally, the number of Gnrh1-expressing cells in the preoptic area was comparable between the groups in both sexes. Our findings revealed that HFD-fed male rats showed KNDy-dependent infertility, while irregular menstruation was mainly induced by KNDy-independent pathways during the incipient stage of obese infertility in female rats. Taken together, hypothalamic kisspeptin neurons in male rats may be susceptible to HFD-induced obesity compared with those in female rats.

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