Specification and Analysis of Multiple Marker Compounds for Quality Control of Mori Cortex Radicis by HPLC

2015 ◽  
Vol 36 (1) ◽  
pp. 117-122 ◽  
Author(s):  
Kyung Tae Kim ◽  
Min Cheol Shin ◽  
Hee Hyun Kim ◽  
Chong Woon Cho ◽  
Won Jae Lee ◽  
...  
Keyword(s):  
Quality Control ◽  
Marker Compounds ◽  
Mori Cortex Radicis

Comparative study of isolated Guggulsterones as marker compounds from Guggulu, Commiphora mukul with Ayurvedic Gugglu containing formulations by HPTLC, an in-house quality control method

2014 ◽  
Vol 1 (1) ◽  
pp. 1 ◽  
Author(s):  
Selvakumar Duraipandi ◽  
Vijaya Selvakumar
Keyword(s):  
Quality Control ◽  
Comparative Study ◽  
Qualitative Analysis ◽  
Control Method ◽  
Raw Material ◽  
Inflammatory Conditions ◽  
Quality Control Method ◽  
Commiphora Mukul ◽  
Marker Compounds ◽  
Hptlc Method

The guggulu containing polyherbal formulations in Ayurveda have been used for treating various inflammatory conditions. A simple HPTLC method has been developed to qualitatively analyze the formulations that claimed to have contained guggulu using the Guggulsterones isolated from the guggulu raw material as a marker. The isolation of Guggulsterones from the resinous gum obtained from the plant was used as a marker to determine the Guggulsterone content in the formulations. The study showed that all the preparations taken for analysis that claimed to contain Guggulsterones was originally having the contents but in variable amounts depending on the amount of resin taken for the preparation. Due to the fact that Guggulsterones are very expensive marker compounds if procured separately as Guggulsterone E and Z forms, this method can be used for routine qualitative analysis of presence of Guggulsterones as an in-house quality control method.

scholarly journals Simultaneous Determination of 5 Flavonoids and 7 Saponins for Quality Control of Traditional Chinese Medicine Preparation Xinnaoshutong Capsule Using HPLC-VWD-ELSD

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Jin Li ◽  
Yang Bai ◽  
Peng Zhang ◽  
Jun He ◽  
Jun Cao ◽  
...  
Keyword(s):  
Quality Control ◽  
Analytical Method ◽  
Mobile Phase ◽  
Gradient Elution ◽  
Tribulus Terrestris ◽  
Ripe Fruit ◽  
Accuracy And Precision ◽  
Marker Compounds ◽  
Traditional Chinese Medicine Preparation

Xinnaoshutong capsule (XC) is a traditional Chinese prescription derived from the ripe fruit of Tribulus terrestris L. (TT). Although XC has long been considered as an important herbal medicine, no analytical method of marker compounds for quality assessment is registered in the Chinese Pharmacopoeia. A simple analytical method of twelve marker components was developed and validated by HPLC-VWD-ELSD method. Chromatographic separation by HPLC was carried out on a Hedera ODS 2 column (4.6 × 250 mm, 5 μm) by gradient elution with acetonitrile-water (0.1% formic acid) as the mobile phase. Various extraction conditions were optimized to achieve twelve marker compounds with faster extraction and higher recovery. The analytical condition was then validated in terms of the linearity, accuracy and precision, repeatability, and stability. The twelve markers were successfully quantified in 30 batches of commercial samples. The developed HPLC-VWD-ELSD could be used as a rapid and reliable way in the assessment and quality control of XC and TT.

Identification, Isolation and Determination of Biomarkers for Quality Control of Bush Tea (Athrixia phyllicoides)

Planta Medica ◽  
2018 ◽  
Vol 84 (12/13) ◽  
pp. 902-912 ◽  
Author(s):  
Lefa Lerotholi ◽  
Sushil Chaudhary ◽  
Weiyang Chen ◽  
Clinton Veale ◽  
Sandra Combrinck ◽  
...  
Keyword(s):  
South Africa ◽  
Quality Control ◽  
Liquid Chromatography ◽  
Ultra Performance Liquid Chromatography ◽  
Mass Spectrometry Data ◽  
Resonance Spectroscopy ◽  
Analysis Model ◽  
Relative Standard ◽  
Marker Compounds

Abstract Athrixia phylicoides, known as “bush tea”, grows abundantly in South Africa. An infusion of the leaves is used as a beverage and to treat a multitude of health conditions. The aim of this study was to investigate the chemical variation within A. phylicoides and to identify characteristic compounds for quality control. Samples from 12 locations in South Africa were analysed using ultra-performance liquid chromatography. Hierarchical cluster analysis of the aligned ultra-performance liquid chromatography-time-of-flight mass spectrometry data indicated two groups on the resulting dendrogram, representing 48 samples. Five marker compounds, identified through visual inspection and the construction of a discriminant analysis model, were evident on the ultra-performance liquid chromatography-MS profiles. Four of these compounds were isolated and identified, three as hydroxy methoxyflavones and the fourth as a coumarate, using nuclear magnetic resonance spectroscopy. An ultra-performance liquid chromatography-photodiode array method was developed and validated for the determination of the marker compounds using the isolates as standards. The limits of detection for the four compounds ranged from 0.92 – 2.50 µg/mL. Their recoveries at three concentration levels (1.00, 10.0, and 100 µg/mL) were between 97.0 and 101%, while acceptable intra- and inter-day precision was obtained as reflected by percentage relative standard deviation values below 2.24%. The concentrations of all the marker compounds were found to be higher in samples corresponding to Group 1 of the dendrogram than in those from Group 2. This may be attributable to differences in altitude, climate, and some edaphic factors. Identification of these marker compounds will make a valuable contribution towards the quality control and sustainable commercialisation of bush tea.

scholarly journals Simultaneous Analysis to Evaluate the Quality of Insamyangpye–Tang Using High-Performance Liquid Chromatography–Photo Diode Array Detection

2021 ◽  
Vol 11 (11) ◽  
pp. 4819
Author(s):  
Chang-Seob Seo ◽  
Mee-Young Lee
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Phase System ◽  
Analysis Method ◽  
Marker Compounds ◽  
Array Detection ◽  
Basic Quality

Insamyangpye–tang (ISYPT) is a traditional medicinal formula comprised of 13 herbs and has been used in East Asia to treat lung-related diseases. However, to our knowledge, no method of analysis for its quality control has been reported. In this study, a method of analysis for quality control of ISYPT was developed using high-performance liquid chromatography. Chromatographic separation, analysis, and assay verification were performed with a distilled water–acetonitrile mobile phase system, both containing 0.1% (v/v) trifluoroacetic acid, and a Gemini C18 analytical column (4.6 mm × 250 mm, 5 mm) using authentic standards for eight marker compounds. These marker constituents were detected simultaneously at 0.09–5.95 mg/g. The analysis method developed can be used for basic quality control of ISYPT.

Development and Validation of a High-Performance Thin-Layer Chromatographic (HPTLC) Method for Simultaneous Quantification of Reserpine, Atropine, and Piperine in Sarpagandha Ghanvati, a Classical Ayurvedic Preparation

2019 ◽  
Vol 102 (4) ◽  
pp. 1021-1026 ◽  
Author(s):  
Kilambi Pundarikakshudu ◽  
Anil Kumar Sharma ◽  
Chaitanya J Bhatt ◽  
Niranjan S Kanaki
Keyword(s):  
Quality Control ◽  
Emotional Disorders ◽  
High Performance ◽  
Simultaneous Estimation ◽  
Hyoscyamus Niger ◽  
Simultaneous Quantification ◽  
Anxiolytic Agent ◽  
Marker Compounds ◽  
Development And Validation ◽  
Hptlc Method

Abstract Background: Anxiety disorders are the most common of emotional disorders, affecting more than 20 million people annually. Sarpagandha Ghanvati is a classical Ayurvedic polyherbal formulation prescribed in conditions of insomnia, hysteria, and is used as an anxiolytic agent. Standardization and quality control are the two major issues that need to be addressed for herbal formulations, especially those containing multiple herbal ingredients. Objective: An HPTLC method was developed for the simultaneous quantification of reserpine, atropine, and piperine from Sarpagandha Ghanvati containing Rauwolfia serpentine (root), Hyoscyamus niger (seed), and Piper longum (root and stem). Methods: The marker compounds were effectively resolved on a silica gel G TLC plate using toluene–ethyl acetate–diethyl amine (7+2+1, v/v) as the mobile phase. The detected wavelengths for reserpine, atropine, and piperine were 269, 220, and 254 nm, respectively. The method was validated as per the International Conference on Harmonization guidelines. Results: R. serpentine roots contained 0.82% w/w of reserpine. Atropine content in the seeds of H. niger was found to be 0.004% w/w, whereas P. longum roots were found to contain 0.508% of piperine. The method was found to be accurate, which was evident from 98.93, 99.46, and 99.10% recovery of reserpine, atropine, and piperine, respectively, when the respective herbs were spiked with them. By the developed HPTLC method, 1.0 g of Sarpagandha Ghanvati was found to contain 4.94, 0.049, and 0.318 mg of reserpine, atropine, and piperine, respectively. The recoveries of these three markers from the formulation were found to be 90.32, 92.45, and 89.97%, respectively. Conclusions: The developed method can be successfully used for simultaneous estimation of these marker compounds and for the quality control of the classical Ayurvedic formulation Sarpagandha Ghanvati. Highlights: This works describes effects of extraction solvents on the quantities of marker compounds in the formulations. It also suggests a simple and reliable HPTLC method for simultaneous quantification of three different marker compounds from a poly-herbal formulation.

scholarly journals A new high-performance liquid chromatographic method for the quality control of bioconverted Mori Folium extracts with appropriate marker compounds related to antidiabetes

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jin Hyeok Kim ◽  
Tae Jung Kim ◽  
Hyun June Kim ◽  
Chong Woon Cho ◽  
Seok Jin Kim ◽  
...  
Keyword(s):  
Quality Control ◽  
Caffeic Acid ◽  
High Performance ◽  
Water Extract ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Chromatography Method ◽  
Marker Compounds ◽  
Liquid Chromatography Method ◽  
Enzymatic Bioconversion

AbstractA HPLC method was developed to assess the enhanced bioactivity of bioconverted Mori Folium (MF) extract and to apply a quality-control system. Liquid-liquid extraction was applied to observe changes in chemical composition through enzymatic bioconversion. The ethyl acetate layer was used for quality control of the anti-diabetic functional MF product. A high-performance liquid chromatography method was developed to analyze five marker compounds (syringaldehyde, trans-caffeic acid, morin 3-O-β-D-glucopyranoside, astragalin, and moracin M 3′-O-β-glucopyranoside) within 60 min. Optimized sample preparation was sonication for 44 min and a water-to-material ratio of 102.5 mL/g by response surface methodology. Comparing MF water extract, trans-caffeic acid and syringaldehyde contents were increased 1.89 times and 2.70 times at Viscozyme-bioconverted material by applying the developed method. This HPLC method can be used to control the quality of bioconverted MF based on suitable bioactive markers and also applied to develop anti-diabetic functional foods containing Mori Folium.

Quantitative analysis of the five marker compounds in Mori Cortex Radicis and its anti-inflammatory effects

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
CS Seo ◽  
HS Lim ◽  
SJ Jeong ◽  
HK Shin
Keyword(s):  
Quantitative Analysis ◽  
Marker Compounds ◽  
Anti Inflammatory ◽  
Mori Cortex Radicis

scholarly journals DNA Metabarcoding for Quality Control of Basil, Oregano, and Paprika

2021 ◽  
Vol 12 ◽  
Author(s):  
Ancuţa Cristina Raclariu-Manolică ◽  
Jarl Andreas Anmarkrud ◽  
Marcin Kierczak ◽  
Nima Rafati ◽  
Birgitte Lisbeth Graae Thorbek ◽  
...  
Keyword(s):  
Quality Control ◽  
Analytical Methods ◽  
False Positives ◽  
Food Sector ◽  
Constituent Species ◽  
Dna Metabarcoding ◽  
Product Authenticity ◽  
Marker Compounds ◽  
Analytical Approaches ◽  
Herbs And Spices

Herbs and spices are some of the most vulnerable products in terms of fraud and adulteration in the food sector. Although standard analytical methods are accurate for quality control of specific lead or marker compounds, they cannot accurately assess the entire species composition of many marketed products. Complementary analytical approaches are thus often used for comprehensive screening of herbs and spices. In this study we evaluate DNA metabarcoding for the identification and authentication of 62 products, containing basil, oregano, and paprika collected from different retailers and importers in Norway. Our results show varying degrees of discrepancy between the constituent species and those listed on the product labels, despite high product authenticity. We suggest the false positives result from the sensitivity of DNA metabarcoding and filtering thresholds should be integrated into protocols to reduce false positives. Our results highlight how integrating DNA metabarcoding into the toolbox of analytical methods for quality control of fresh and/or processed plant-based food can improve product quality.

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