Down-regulation of G protein-coupled receptor 137 by RNA interference inhibits cell growth of two hepatoma cell lines

2015 ◽  
Vol 39 (4) ◽  
pp. 418-426 ◽  
Author(s):  
Xin Shao ◽  
Yong Liu ◽  
Hai Huang ◽  
Linyuan Zhuang ◽  
Tianping Luo ◽  
...  
Keyword(s):  
Rna Interference ◽  
Cell Growth ◽  
G Protein ◽  
Cell Lines ◽  
Hepatoma Cell ◽  
G Protein Coupled Receptor ◽  
Down Regulation ◽  
Hepatoma Cell Lines ◽  
G Protein Coupled

scholarly journals Genome-Wide RNA Interference in Drosophila Cells Identifies G Protein-Coupled Receptor Kinase 2 as a Conserved Regulator of NF-κB Signaling

2010 ◽  
Vol 184 (11) ◽  
pp. 6188-6198 ◽  
Author(s):  
Susanna Valanne ◽  
Henna Myllymäki ◽  
Jenni Kallio ◽  
Martin Rudolf Schmid ◽  
Anni Kleino ◽  
...  
Keyword(s):  
Rna Interference ◽  
G Protein ◽  
Receptor Kinase ◽  
G Protein Coupled Receptor ◽  
Genome Wide ◽  
G Protein Coupled ◽  
Drosophila Cells

scholarly journals Role of the G-Protein-Coupled Receptor Signaling Pathway in Insecticide Resistance

2019 ◽  
Vol 20 (17) ◽  
pp. 4300 ◽  
Author(s):  
Ting Li ◽  
Nannan Liu
Keyword(s):  
Insecticide Resistance ◽  
Cell Line ◽  
Signaling Pathway ◽  
G Protein ◽  
Cell Lines ◽  
Sf9 Cells ◽  
Camp Production ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

The G-protein-coupled receptor (GPCR) regulated intracellular signaling pathway is known to be involved in the development of insecticide resistance in the mosquito, Culex quinquefasciatus. To elucidate the specific role of each effector in the GPCR regulating pathway, we initially expressed a GPCR, G-protein alpha subunit (Gαs), adenylate cyclase (AC), and protein kinase A (PKA) in insect Spodoptera frugiperda (Sf9) cells and investigated their regulation function on cyclic AMP (cAMP) production and PKA activity. GPCR, Gαs, and AC individually expressed Sf9 cells showed higher cAMP production as the expression of each effector increased. All the effector-expressed cell lines showed increased PKA activity however. Moreover, Sf9 cytochrome P450 gene expression and cell tolerance to permethrin were examined. The relative expression of CYP9A32gene in Sf9 cells tested was significantly increased in all effector-expressed cell lines compared to a control cell line; these effector-expressed cell lines also showed significantly higher tolerance to permethrin. Inhibitor treatments on each effector-expressed cell line revealed that Bupivacaine HCl and H89 2HCl robustly inhibited cAMP production and PKA activity, respectively, resulting in decreased tolerance to permethrin in all cell lines. The synergistic functions of Bupivacaine HCl and H89 2HCl with permethrin were further examined in Culex mosquito larvae, providing a valuable new information for mosquito control strategies.

The obestatin/G-protein coupled receptor 39 (GPR39) system in pancreatic cancer: proliferation studies in pancreatic cancer cell lines

Pancreatology ◽  
2019 ◽  
Vol 19 ◽  
pp. S162
Author(s):  
Lara Estevez-Perez ◽  
Saul Leal-Lopez ◽  
Vanesa Veloso-Noya ◽  
Begoña Otero-Alen ◽  
Carlos Seoane-Mosteiro ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
G Protein ◽  
Cell Lines ◽  
Cancer Cell ◽  
Pancreatic Cancer Cell ◽  
Cancer Cell Lines ◽  
G Protein Coupled Receptor ◽  
Cancer Proliferation ◽  
G Protein Coupled

scholarly journals Down regulation of G protein-coupled receptor 137 expression inhibits proliferation and promotes apoptosis in leukemia cells

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Li-Jie Men ◽  
Ji-Zhu Liu ◽  
Hai-Ying Chen ◽  
Li Zhang ◽  
Shuang-Feng Chen ◽  
...  
Keyword(s):  
G Protein ◽  
Leukemia Cells ◽  
G Protein Coupled Receptor ◽  
Down Regulation ◽  
G Protein Coupled

scholarly journals Adjuvant Arthritis Induces Down-Regulation of G Protein-Coupled Receptor Kinases in the Immune System

2001 ◽  
Vol 166 (3) ◽  
pp. 1635-1640 ◽  
Author(s):  
Maria Stella Lombardi ◽  
Annemieke Kavelaars ◽  
Pieter M. Cobelens ◽  
Reinhold E. Schmidt ◽  
Manfred Schedlowski ◽  
...  
Keyword(s):  
Immune System ◽  
G Protein ◽  
Adjuvant Arthritis ◽  
G Protein Coupled Receptor ◽  
Down Regulation ◽  
Receptor Kinases ◽  
G Protein Coupled

scholarly journals Inhibition of G protein-coupled receptor kinase 2 promotes unbiased downregulation of IGF-1 receptor and restrains malignant cell growth

2020 ◽  
pp. canres.1662.2020
Author(s):  
Caitrin Crudden ◽  
Takashi Shibano ◽  
Dawei Song ◽  
Mihnea P. Dragomir ◽  
Sonia Cismas ◽  
...  
Keyword(s):  
Cell Growth ◽  
G Protein ◽  
Malignant Cell ◽  
Receptor Kinase ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled
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