scholarly journals Cover Feature: A New One‐Pot Fluorescence Derivatization Strategy for Highly Sensitive MicroRNA Analysis (Chem. Eur. J. 25/2020)

2020 ◽  
Vol 26 (25) ◽  
pp. 5554-5554
Author(s):  
Li Pan ◽  
Huaisheng Zhang ◽  
Jingjin Zhao ◽  
Ifedayo Victor Ogungbe ◽  
Shulin Zhao ◽  
...  
Keyword(s):  
Fluorescence Derivatization ◽  
One Pot ◽  
Highly Sensitive

A New One‐Pot Fluorescence Derivatization Strategy for Highly Sensitive MicroRNA Analysis

2020 ◽  
Vol 26 (25) ◽  
pp. 5639-5647
Author(s):  
Li Pan ◽  
Huaisheng Zhang ◽  
Jingjin Zhao ◽  
Ifedayo Victor Ogungbe ◽  
Shulin Zhao ◽  
...  
Keyword(s):  
Fluorescence Derivatization ◽  
One Pot ◽  
Highly Sensitive

scholarly journals A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Guanhua Xun ◽  
Stephan Thomas Lane ◽  
Vassily Andrew Petrov ◽  
Brandon Elliott Pepa ◽  
Huimin Zhao
Keyword(s):  
Limit Of Detection ◽  
Low Cost ◽  
High Volume ◽  
N Gene ◽  
Testing System ◽  
Target Sequence ◽  
One Pot ◽  
Sequence Detection ◽  
Highly Sensitive ◽  
Speed Accuracy

AbstractThe need for rapid, accurate, and scalable testing systems for COVID-19 diagnosis is clear and urgent. Here, we report a rapid Scalable and Portable Testing (SPOT) system consisting of a rapid, highly sensitive, and accurate assay and a battery-powered portable device for COVID-19 diagnosis. The SPOT assay comprises a one-pot reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) followed by PfAgo-based target sequence detection. It is capable of detecting the N gene and E gene in a multiplexed reaction with the limit of detection (LoD) of 0.44 copies/μL and 1.09 copies/μL, respectively, in SARS-CoV-2 virus-spiked saliva samples within 30 min. Moreover, the SPOT system is used to analyze 104 clinical saliva samples and identified 28/30 (93.3% sensitivity) SARS-CoV-2 positive samples (100% sensitivity if LoD is considered) and 73/74 (98.6% specificity) SARS-CoV-2 negative samples. This combination of speed, accuracy, sensitivity, and portability will enable high-volume, low-cost access to areas in need of urgent COVID-19 testing capabilities.

scholarly journals Highly Sensitive Fluorescent Detection of Acetylcholine Based on the Enhanced Peroxidase-Like Activity of Histidine Coated Magnetic Nanoparticles

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1207
Author(s):  
Hong Jae Cheon ◽  
Quynh Huong Nguyen ◽  
Moon Il Kim
Keyword(s):  
Magnetic Nanoparticles ◽  
High Sensitivity ◽  
High Specificity ◽  
Choline Oxidase ◽  
Fluorescent Detection ◽  
One Pot ◽  
Site Structure ◽  
Specificity And Sensitivity ◽  
Highly Sensitive ◽  
Peroxidase Mimics

Inspired by the active site structure of natural horseradish peroxidase having iron as a pivotal element with coordinated histidine residues, we have developed histidine coated magnetic nanoparticles (His@MNPs) with relatively uniform and small sizes (less than 10 nm) through one-pot heat treatment. In comparison to pristine MNPs and other amino acid coated MNPs, His@MNPs exhibited a considerably enhanced peroxidase-imitating activity, approaching 10-fold higher in catalytic reactions. With the high activity, His@MNPs then were exploited to detect the important neurotransmitter acetylcholine. By coupling choline oxidase and acetylcholine esterase with His@MNPs as peroxidase mimics, target choline and acetylcholine were successfully detected via fluorescent mode with high specificity and sensitivity with the limits of detection down to 200 and 100 nM, respectively. The diagnostic capability of the method is demonstrated by analyzing acetylcholine in human blood serum. This study thus demonstrates the potential of utilizing His@MNPs as peroxidase-mimicking nanozymes for detecting important biological and clinical targets with high sensitivity and reliability.

A facile one-pot synthesis of polyethyleneimine functionalized graphene for the highly-sensitive and selective electrochemical impedance aptasensing of kanamycin in serum

2020 ◽  
Vol 12 (2) ◽  
pp. 132-140 ◽  
Author(s):  
Yuqing Duan ◽  
Yuting Yang ◽  
Jiangxia Wang ◽  
Huiming Li ◽  
Kunping Liu
Keyword(s):  
Electrochemical Impedance ◽  
Functionalized Graphene ◽  
One Pot ◽  
One Pot Synthesis ◽  
Highly Sensitive

A facile one-pot synthesis of polyethyleneimine-functionalized graphene as well as a highly-sensitive and selective electrochemical impedance aptasensing of kanamycin in serum.

scholarly journals Novel fluorescein polymer-based nanoparticles: facile and controllable one-pot synthesis, assembly, and immobilization of biomolecules for application in a highly sensitive biosensor

RSC Advances ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 2998-3004
Author(s):  
Jiseob Woo ◽  
Heesun Park ◽  
Yoonhee Na ◽  
Sunghyun Kim ◽  
Won Il Choi ◽  
...  
Keyword(s):  
Polymeric Nanoparticles ◽  
Optical Biosensor ◽  
One Pot ◽  
One Pot Synthesis ◽  
Highly Sensitive

We report a facile approach for the development of novel fluorescein polymeric nanoparticles (FPNPs) with immobilization of specific biomolecules for application in a highly sensitive optical biosensor.

Sign in / Sign up

Export Citation Format

Share Document