Characteristic profiles of lipid classes, fatty acids and triacylglycerol molecular species of peas (Pisum sativum L.)

2007 ◽  
Vol 109 (6) ◽  
pp. 600-607 ◽  
Author(s):  
Hiromi Yoshida ◽  
Yuka Tomiyama ◽  
Megumi Tanaka ◽  
Yoshiyuki Mizushina
Keyword(s):  
Fatty Acids ◽  
Pisum Sativum ◽  
Molecular Species ◽  
Lipid Classes ◽  
Pisum Sativum L ◽  
Triacylglycerol Molecular Species

scholarly journals Analysis of Genetic Variation and Enhancement of Salt Tolerance in French Pea (Pisum Sativum L.)

2018 ◽  
Vol 19 (8) ◽  
pp. 2433 ◽  
Author(s):  
Mohamed El-Esawi ◽  
Abdullah Al-Ghamdi ◽  
Hayssam Ali ◽  
Aisha Alayafi ◽  
Jacques Witczak ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antioxidant Enzymes ◽  
Salt Tolerance ◽  
Pisum Sativum ◽  
Crude Oil ◽  
Oil Content ◽  
High Salinity ◽  
Aflp Markers ◽  
Pisum Sativum L ◽  
Trait Associations

Pisum sativum L. (field pea) is a crop of a high nutritional value and seed oil content. The characterization of pea germplasm is important to improve yield and quality. This study aimed at using fatty acid profiling and amplified fragment length polymorphism (AFLP) markers to evaluate the variation and relationships of 25 accessions of French pea. It also aimed to conduct a marker-trait associations analysis using the crude oil content as the target trait for this analysis, and to investigate whether 5-aminolevulinic acid (ALA) could enhance salt tolerance in the pea germplasm. The percentage of crude oil of the 25 pea genotypes varied from 2.6 to 3.5%, with a mean of 3.04%. Major fatty acids in all of the accessions were linoleic acid. Moreover, the 12 AFLP markers used were polymorphic. The cluster analysis based on fatty acids data or AFLP data divided the 25 pea germplasm into two main clusters. The gene diversity of the AFLP markers varied from 0.21 to 0.58, with a mean of 0.41. Polymorphic information content (PIC) of pea germplasm varied from 0.184 to 0.416 with a mean of 0.321, and their expected heterozygosity (He) varied from 0.212 to 0.477 with a mean of 0.362. The AFLP results revealed that the Nain Ordinaire cultivar has the highest level of genetic variability, whereas Elatius 3 has the lowest level. Three AFLP markers (E-AAC/M-CAA, E-AAC/M-CAC, and E-ACA/M-CAG) were significantly associated with the crude oil content trait. The response of the Nain Ordinaire and Elatius 3 cultivars to high salinity stress was studied. High salinity (150 mM NaCl) slightly reduced the photosynthetic pigments contents in Nain Ordinaire leaves at a non-significant level, however, the pigments contents in the Elatius 3 leaves were significantly reduced by high salinity. Antioxidant enzymes (APX—ascorbate peroxidase; CAT—catalase; and POD—peroxidase) activities were significantly induced in the Nain Ordinaire cultivar, but non-significantly induced in Elatius 3 by high salinity. Priming the salt-stressed Nain Ordinaire and Elatius 3 plants with ALA significantly enhanced the pigments biosynthesis, antioxidant enzymes activities, and stress-related genes expression, as compared to the plants stressed with salt alone. In conclusion, this study is amongst the first investigations that conducted marker-trait associations in pea, and revealed a sort of correlation between the diversity level and salt tolerance.

Lipid Classes, Fatty Acid Distributions and Triacylglycerol Molecular Species of Broad Beans (Vicia faba)

2008 ◽  
Vol 85 (6) ◽  
pp. 535-541 ◽  
Author(s):  
Hiromi Yoshida ◽  
Yuka Tomiyama ◽  
Naoko Yoshida ◽  
Masayuki Saiki ◽  
Yoshiyuki Mizushina
Keyword(s):  
Fatty Acid ◽  
Vicia Faba ◽  
Molecular Species ◽  
Lipid Classes ◽  
Broad Beans ◽  
Triacylglycerol Molecular Species

Lipid components, fatty acids and triacylglycerol molecular species of black and red rices

2010 ◽  
Vol 123 (2) ◽  
pp. 210-215 ◽  
Author(s):  
Hiromi Yoshida ◽  
Yuka Tomiyama ◽  
Yoshiyuki Mizushina
Keyword(s):  
Fatty Acids ◽  
Molecular Species ◽  
Triacylglycerol Molecular Species ◽  
Lipid Components

scholarly journals Profiles of Lipid Components, Fatty Acids and Triacylglycerol Molecular Species in Lipids of Rice Bran Cultivars

2012 ◽  
Vol 18 (2) ◽  
pp. 219-226 ◽  
Author(s):  
Hiromi YOSHIDA ◽  
Isoko KURIYAMA ◽  
Yuka TOMIYAMA-SAKAMOTO ◽  
Yoshiyuki MIZUSHINA
Keyword(s):  
Fatty Acids ◽  
Rice Bran ◽  
Molecular Species ◽  
Triacylglycerol Molecular Species ◽  
Lipid Components

Distribution of fatty acids in triacylglycerols and phospholipids from peas (Pisum sativum L.)

2007 ◽  
Vol 87 (14) ◽  
pp. 2709-2714 ◽  
Author(s):  
Hiromi Yoshida ◽  
Yuka Tomiyama ◽  
Megumi Tanaka ◽  
Yoshiyuki Mizushina
Keyword(s):  
Fatty Acids ◽  
Pisum Sativum ◽  
Pisum Sativum L

LIPID CLASSES, FATTY ACID COMPOSITIONS AND TRIACYLGLYCEROL MOLECULAR SPECIES FROM ADZUKI BEANS (VIGNA ANGULARIS)

2008 ◽  
Vol 15 (3) ◽  
pp. 343-355 ◽  
Author(s):  
HIROMI YOSHIDA ◽  
YUKA TOMIYAMA ◽  
NAOKO YOSHIDA ◽  
MASAYUKI SAIKI ◽  
YOSHIYUKI MIZUSHINA
Keyword(s):  
Fatty Acid ◽  
Molecular Species ◽  
Lipid Classes ◽  
Vigna Angularis ◽  
Triacylglycerol Molecular Species ◽  
Fatty Acid Compositions

scholarly journals Vitamin E Profiles and Triacylglycerol Molecular Species of Colored Rice Bran Cultivars at Different Degree of Milling

2013 ◽  
Vol 2 (6) ◽  
pp. 106
Author(s):  
Hiromi Yoshida ◽  
Mika Kanamori ◽  
Naoko Yoshida ◽  
Yuka Sakamoto ◽  
Isoko Kuriyama ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Vitamin E ◽  
Free Fatty Acids ◽  
Rice Bran ◽  
Molecular Species ◽  
Alpha Tocopherol ◽  
Positive Health ◽  
Degree Of Milling ◽  
Triacylglycerol Molecular Species ◽  
Colored Rice

The objective of this study was to evalute the tocochromanol distributions, lipid components and molecular species of triacylglycerols (TAG) in three colored rice bran cultivars. The dominant tocochromanol were gama-tocotrienol, alpha-tocopherol and alpha-tocotrienol with smaller amounts of gama-tocopherol, delta-tocopherol and delta-tocotrienol. These lipids comprised mainly TAG (78.0-81.6 wt%), free fatty acids (FFA: 5.6-8.8 wt%), and phospholipids (PL: 6.3-7.0 wt%), while other components were present in minor proportions (0.4-2.3 wt%). Sixteen different TAG molecular species were detected and quantified by successive applications of AgNO<sub>3</sub>-TLC and GC. The major TAG molecular species were SM<sub>2 </sub>(6.1-9.8%), S<sub>2</sub>D (4.8-7.3%)?M<sub>3</sub> (16.4-18.7%), SMD (6.2-9.2%), SD<sub>2</sub> (6.5-9.5%), SMT (6.3-7.7%), M<sub>2</sub>D (12.3-15.5%.), MD<sub>2</sub> (8.4-10.4%), SDT (4.3-5.4%) and D<sub>3</sub> (10.2-15.2%) (where S, M, D, and T denote saturated FA, monoene, diene, and triene, respectively). The results showed that colored rice bran lipids contain large amounts of nutraceutical with proven positive health effects.

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