Application of esterase inhibitors: A possible new approach to protect unsaturated fatty acids from ruminal biohydrogenation

2015 ◽  
Vol 117 (10) ◽  
pp. 1667-1672 ◽  
Author(s):  
Mohammad Mahdi Sargolzehi ◽  
Abbasali Naserian ◽  
Ahmad Asoodeh ◽  
Masoumeh Rezaee Roknabadi ◽  
Jong Suh Shin ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
New Approach ◽  
Esterase Inhibitors ◽  
Ruminal Biohydrogenation

Effect of different forms of polyunsaturated fatty acids on duodenal and serum fatty acid profiles in sheep

1994 ◽  
Vol 74 (4) ◽  
pp. 595-600 ◽  
Author(s):  
F. Enjalbert ◽  
M. C. Nicot ◽  
D. Griess ◽  
M. Vernay ◽  
R. Moncoulon
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Unsaturated Fatty Acids ◽  
Vaccenic Acid ◽  
Soy Oil ◽  
Ruminal Fermentation ◽  
Serum Fatty Acid ◽  
Calcium Salts ◽  
Physical Form ◽  
Ruminal Biohydrogenation

Four sheep cannulated in the rumen and proximal duodenum were used in a 4 × 4 cross-over design to investigate the effects of ruminal fatty acid (FA) infusion on duodenal and serum FA profiles. The diets were composed of 85.7% natural grassland hay and 8.6% concentrate supplemented with 5.7% soy oil for diet SO, 5.7% emulsified soy oil for diet ESO, 6.7% calcium salts of soy or palm FA for diets CaSSO and CaSP, respectively. Diets were formulated to be isonitrogenous and isoenergetic; total FA content in dry matter was 6.4–6.6%. Characteristics of ruminal fermentation were not affected by source or physical form of FA. The proportion of stearic acid in the duodenal flow (% of the total C18) was high compared with total diet, e.g., 49.8 vs. 3.5 and 54.3 vs. 9.4% for soy and palm diets, respectively. Ruminal biohydrogenation and unsaturated FA was lower for CaS diets than for SO and ESO diets (48.7 and 60.9 vs. 81.2 and 94.7%, for oleic and linoleic acids, respectively). As a result, trans-vaccenic acid levels in duodenal flow and serum (% or total FA) were lower for the CaS diets than for SO and ESO diets (8.3 vs. 36.0% and 0.9 vs. 7.8%, respectively). Unsaturated FA as CaS were partly protected against ruminal biohydrogenation, and can be effective in increasing intestinal absorption of unsaturated FA. Key words: Unsaturated fatty acids, soy oil, calcium salts, biohydrogenation, sheep

scholarly journals Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids

2006 ◽  
Vol 95 (6) ◽  
pp. 1199-1211 ◽  
Author(s):  
I. Wąsowska ◽  
M. R. G. Maia ◽  
K. M. Niedźwiedzka ◽  
M. Czauderna ◽  
J. M. C. Ramalho Ribeiro ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Fish Oil ◽  
Unsaturated Fatty Acids ◽  
Butyrivibrio Fibrisolvens ◽  
Isomerase Activity ◽  
Epa And Dha ◽  
Pure Cultures ◽  
Ruminal Biohydrogenation

Dietarycis-9,trans-11-conjugated linoleic acid (CLA) is generally thought to be beneficial for human health. Fish oil added to ruminant diets increases the CLA concentration of milk and meat, an increase thought to arise from alterations in ruminal biohydrogenation of unsaturated fatty acids. To investigate the mechanism for this effect,in vitroincubations were carried out with ruminal digesta and the main biohydrogenating ruminal bacterium,Butyrivibrio fibrisolvens. Linoleic acid (LA) or α-linolenic acid (LNA) was incubated (1·67g/l) with strained ruminal digesta from sheep receiving a 50:50 grass hay–concentrate ration. Adding fish oil (up to 4·17g/l) tended to decrease the initial rate of LA (P=0·025) and LNA (P=0·137) disappearance, decreased (P<0·05) the transient accumulation of conjugated isomers of both fatty acids, and increased (P<0·05) the accumulation oftrans-11-18:1. Concentrations of EPA (20:5n-3) or DHA (22:6n-3), the major fatty acids in fish oil, were low (100mg/l or less) after incubation of fish oil with ruminal digesta. Addition of EPA or DHA (50mg/l) to pure cultures inhibited the growth and isomerase activity ofB. fibrisolvens, while fish oil had no effect. In contrast, similar concentrations of EPA and DHA had no effect on biohydrogenation of LA by mixed digesta, while the addition of LA prevented metabolism of EPA and DHA. Neither EPA nor DHA was metabolised byB. fibrisolvensin pure culture. Thus, fish oil inhibits ruminal biohydrogenation by a mechanism which can be interpreted partly, but not entirely, in terms of its effects onB. fibrisolvens.

Effects of dietary calcium soaps of unsaturated fatty acids on digestion, milk composition and physical properties of butter

1997 ◽  
Vol 64 (2) ◽  
pp. 181-195 ◽  
Author(s):  
FRANCIS ENJALBERT ◽  
MARIE CLAUDE NICOT ◽  
CORINE BAYOURTHE ◽  
MICHELE VERNAY ◽  
RAYMOND MONCOULON
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Milk Fat ◽  
Dry Matter ◽  
Unsaturated Fatty Acids ◽  
Control Diet ◽  
Milk Composition ◽  
Ruminal Fermentation ◽  
Maize Silage ◽  
Ruminal Biohydrogenation

Dairy cows fitted with ruminal, duodenal and ileal cannulas were utilized to investigate the effects of feeding with Ca soaps (CaS) of palm fatty acids (FA) and rapeseed FA. Diets compared were control diet based on maize silage and concentrate, and two diets with 40 g CaS of palm oil FA or rapeseed oil FA/kg diet, replacing part of the concentrates of the control diet. Total digestibilities of dry matter, fibre and fat, and ruminal fermentation were not significantly altered by giving CaS; the extent of ruminal biohydrogenation of total unsaturated C18 FA was significantly reduced by both CaS diets. Apparent intestinal digestibility of FA was not different among diets, although the amount of FA absorbed with the CaS diets was twice that with the control diet. No difference among diets was observed for milk production, or fat and protein contents. Giving CaS diets decreased the proportions of 4[ratio ]0 to 14[ratio ]0 FA in milk fat, and increased cis-18[ratio ]1n−9, compared with control diet. The rapeseed diet lowered the content of 16[ratio ]0, and increased the contents of 18[ratio ]0 and trans-18[ratio ]1n−7. CaS diets did not result in a marked increase of polyunsaturated FA content in milk fat. Butter from cows fed on the CaS diets contained more liquid fat at 6 and 14°C than butter from the cows fed on the control diet. Incorporating CaS, particularly those from rapeseed, in dairy cows' diets increased C18 FA in milk and improved butter spreadability.

scholarly journals In Vitro Versus in Situ Ruminal Biohydrogenation of Unsaturated Fatty Acids from a Raw or Extruded Mixture of Ground Canola Seed/Canola Meal

2003 ◽  
Vol 86 (1) ◽  
pp. 351-359 ◽  
Author(s):  
F. Enjalbert ◽  
P. Eynard ◽  
M.C. Nicot ◽  
A. Troegeler-Meynadier ◽  
C. Bayourthe ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Canola Meal ◽  
Canola Seed ◽  
Ruminal Biohydrogenation

scholarly journals Effect of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids in steers fed grass or red clover silages

animal ◽  
2008 ◽  
Vol 2 (12) ◽  
pp. 1859-1869 ◽  
Author(s):  
M.R.F. Lee ◽  
K.J. Shingfield ◽  
J.K.S. Tweed ◽  
V. Toivonen ◽  
S.A. Huws ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fish Oil ◽  
Unsaturated Fatty Acids ◽  
Red Clover ◽  
Ruminal Biohydrogenation

Ruminal biohydrogenation of unsaturated fatty acids in vitro as affected by chitosan

2010 ◽  
Vol 159 (1-2) ◽  
pp. 35-40 ◽  
Author(s):  
I. Goiri ◽  
G. Indurain ◽  
K. Insausti ◽  
V. Sarries ◽  
A. Garcia-Rodriguez
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Ruminal Biohydrogenation

DEVELOPMENT OF AN EXPERIMENTAL MODEL OF AVITAMINOSIS F

2020 ◽  
Vol 20 (2) ◽  
pp. 38-40
Author(s):  
A. Levitsky ◽  
A. Lapinska ◽  
I. Selivanskaya
Keyword(s):  
Fatty Acids ◽  
Experimental Model ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Coconut Oil ◽  
The Body ◽  
Regulatory Function ◽  
Omega 3 ◽  
White Rats ◽  
Arachidonic Acids

The article analyzes the role of essential polyunsaturated fatty acids (PUFA), especially omega-3 series in humans and animals. The biosynthesis of essential PUFA in humans and animals is very limited, so they must be consumed with food (feed). Тhe ratio of omega-3 and omega-6 PUFA is very important. Biomembranes of animal cells contain about 30% PUFA with a ratio of ω-6/ ω-3 1-2. As this ratio increases, the physicochemical properties of biomembranes and the functional activity of their receptors change. The regulatory function of essential PUFA is that in the body under the action of oxygenase enzymes (cyclooxygenase, lipoxygenase) are formed extremely active hormone-like substances (eicosanoids and docosanoids), which affect a number of physiological processes: inflammation, immunity, metabolism. Moreover, ω-6 PUFA form eicosanoids, which have pro-inflammatory, immunosuppressive properties, and ω-3 PUFAs form eicosanoids and docosanoids, which have anti-inflammatory and immunostimulatory properties. Deficiency of essential PUFA, and especially ω-3 PUFA, leads to impaired development of the body and its state of health, which are manifestations of avitaminosis F. Prevention and treatment of avitaminosis F is carried out with drugs that contain PUFA. To create new, more effective vitamin F preparations, it is necessary to reproduce the model of vitamin F deficiency. An experimental model of vitamin F deficiency in white rats kept on a fat –free diet with the addition of coconut oil, which is almost completely free of unsaturated fatty acids, and saturated fatty acids make up almost 99 % of all fatty acids was developed. The total content of ω-6 PUFA (sum of linoleic and arachidonic acids), the content of ω-3 PUFA (α-linolenic, eicosapentaenoic and docosahexaenoic acids) in neutral lipids (triglycerides and cholesterol esters) defined. Тhe content of ω-6 PUFA under the influence of coconut oil decreased by 3.3 times, and the content of ω-3 PUFA - by 7.5 times. Тhe influence of coconut oil, the content of ω-6 PUFA decreased by 2.1 times, and the content of ω-3 PUFA - by 2.8 times. The most strongly reduces the content of ω-3 PUFA, namely eicosapentaenoic, coconut oil, starting from 5 %. Consumption of FFD with a content of 15 % coconut oil reduces the content of eicosapentaenoic acid to zero, ie we have an absolute deficiency of one of the most important essential PUFAs, which determined the presence of vitamin F deficiency.

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