Effect of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids in steers fed grass or red clover silages

Dietarycis-9,trans-11-conjugated linoleic acid (CLA) is generally thought to be beneficial for human health. Fish oil added to ruminant diets increases the CLA concentration of milk and meat, an increase thought to arise from alterations in ruminal biohydrogenation of unsaturated fatty acids. To investigate the mechanism for this effect,in vitroincubations were carried out with ruminal digesta and the main biohydrogenating ruminal bacterium,Butyrivibrio fibrisolvens. Linoleic acid (LA) or α-linolenic acid (LNA) was incubated (1·67g/l) with strained ruminal digesta from sheep receiving a 50:50 grass hay–concentrate ration. Adding fish oil (up to 4·17g/l) tended to decrease the initial rate of LA (P=0·025) and LNA (P=0·137) disappearance, decreased (P<0·05) the transient accumulation of conjugated isomers of both fatty acids, and increased (P<0·05) the accumulation oftrans-11-18:1. Concentrations of EPA (20:5n-3) or DHA (22:6n-3), the major fatty acids in fish oil, were low (100mg/l or less) after incubation of fish oil with ruminal digesta. Addition of EPA or DHA (50mg/l) to pure cultures inhibited the growth and isomerase activity ofB. fibrisolvens, while fish oil had no effect. In contrast, similar concentrations of EPA and DHA had no effect on biohydrogenation of LA by mixed digesta, while the addition of LA prevented metabolism of EPA and DHA. Neither EPA nor DHA was metabolised byB. fibrisolvensin pure culture. Thus, fish oil inhibits ruminal biohydrogenation by a mechanism which can be interpreted partly, but not entirely, in terms of its effects onB. fibrisolvens.

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EFFECT OF GINGER, LEMURU FISH OIL SAPONIFICATION AND OLIVE OIL ON COMPOSITION FATTY ACID OF BEEF

Jurnal Agroindustri ◽

10.31186/j.agroind.4.1.31-39 ◽

2014 ◽

Vol 4 (1) ◽

pp. 31-39

Author(s):

Siwitri Kadarsih

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Fish Oil ◽

Olive Oil ◽

Rice Bran ◽

Dry Matter ◽

Unsaturated Fatty Acids ◽

Saturated Fatty Acids ◽

Omega 3 ◽

Omega 6

The objective was to get beef that contain unsaturated fatty acids (especially omega 3 and 6), so as to improve intelligence, physical health for those who consume. The study design using CRD with 3 treatments, each treatment used 4 Bali cattle aged approximately 1.5 years. Observations were made 8 weeks. Pasta mixed with ginger provided konsentrat. P1 (control); P2 (6% saponification lemuru fish oil, olive oil 1%; rice bran: 37.30%; corn: 62.70%; KLK: 7%, ginger paste: 100 g); P3 (lemuru fish oil saponification 8%, 2% olive oil; rice bran; 37.30; corn: 62.70%; KLK: 7%, ginger paste: 200 g). Konsentrat given in the morning as much as 1% of the weight of the cattle based on dry matter, while the grass given a minimum of 10% of the weight of livestock observation variables include: fatty acid composition of meat. Data the analyzies qualitative. The results of the study showed that the composition of saturated fatty acids in meat decreased and an increase in unsaturated fatty acids, namely linoleic acid (omega 6) and linolenic acid (omega 3), and deikosapenta deikosaheksa acid.Keywords :

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Effect of incremental amounts of fish oil in the diet on ruminal lipid metabolism in growing steers

British Journal Of Nutrition ◽

10.1017/s0007114510000292 ◽

2010 ◽

Vol 104 (1) ◽

pp. 56-66 ◽

Cited By ~ 26

Author(s):

K. J. Shingfield ◽

M. R. F. Lee ◽

D. J. Humphries ◽

N. D. Scollan ◽

V. Toivonen ◽

...

Keyword(s):

Fatty Acids ◽

Lipid Metabolism ◽

Fish Oil ◽

Latin Square ◽

Live Weight ◽

Dependent Manner ◽

Growing Cattle ◽

Potential Benefits ◽

Ruminal Biohydrogenation ◽

Long Chain

Based on the potential benefits to human health, there is interest in developing sustainable nutritional strategies to enhance the concentration of long-chainn-3 fatty acids in ruminant-derived foods. Four Aberdeen Angus steers fitted with rumen and duodenal cannulae were used in a 4 × 4 Latin square experiment with 21 d experimental periods to examine the potential of fish oil (FO) in the diet to enhance the supply of 20 : 5n-3 and 22 : 6n-3 available for absorption in growing cattle. Treatments consisted of total mixed rations based on maize silage fed at a rate of 85 g DM/kg live weight0·75/d containing 0, 8, 16 and 24 g FO/kg diet DM. Supplements of FO reduced linearly (P < 0·01) DM intake and shifted (P < 0·01) rumen fermentation towards propionate at the expense of acetate and butyrate. FO in the diet enhanced linearly (P < 0·05) the flow oftrans-16 : 1,trans-18 : 1,trans-18 : 2, 20 : 5n-3 and 22 : 6n-3, and decreased linearly (P < 0·05) 18 : 0 and 18 : 3n-3 at the duodenum. Increases in the flow oftrans-18 : 1 were isomer dependent and were determined primarily by higher amounts oftrans-11 reaching the duodenum. In conclusion, FO alters ruminal lipid metabolism of growing cattle in a dose-dependent manner consistent with an inhibition of ruminal biohydrogenation, and enhances the amount of long-chainn-3 fatty acids at the duodenum, but the increases are marginal due to extensive biohydrogenation in the rumen.

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Acute postprandial effect of hydrogenated fish oil, palm oil and lard on plasma cholesterol, triacylglycerol and non-esterified fatty acid metabolism in normocholesterolaemic males

British Journal Of Nutrition ◽

10.1079/bjn20051723 ◽

2006 ◽

Vol 95 (4) ◽

pp. 787-794 ◽

Cited By ~ 4

Author(s):

Marie M. Cantwell ◽

Mary A.T. Flynn ◽

Michael J. Gibney

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Fish Oil ◽

Palm Oil ◽

Unsaturated Fatty Acids ◽

Lipid Analysis ◽

Plasma Cholesterol ◽

Random Order ◽

Postprandial Response ◽

Chain Fatty Acids

The majority of research has focused on the association betweentransunsaturated fatty acids (TUFA) from hydrogenated vegetable oils and heart disease even though TUFA are also produced from hydrogenated fish oil. We compared the acute effect of three solid fats on postprandial cholesterol, triacylglycerol (TAG) and NEFA concentrations in normocholesterolaemic males. Eight healthy male volunteers consumed each of the three 40g fat meals (partially hydrogenated fish oil (PHFO), palm oil and lard) in random order and blood samples were drawn at 2, 4, 6 and 8h thereafter for lipid analysis. The postprandial response in plasma TAG, TAG-rich lipoprotein-TAG (TRL-TAG), total cholesterol and plasma NEFA, measured as the area under the postprandial curve, was not significantly different between the three meals (p>0·05), which varied in MUFA, PUFA and TUFA content. There was no marked elevation of longer-chain fatty acids (C20–22,cisortransisomers) into the TRL-TAG fraction following the PHFO meal even though they provided 40% of the total fatty acids in the PHFO meal. The postprandial TRL-TAG response to PHFO was expected to be higher, as it is higher in TUFA, lower in PUFA and similar in saturated fatty acid composition compared with the lard and palm oil test meals. The absence of a higher postprandial response following ingestion of PHFO could be as a result of reduced absorption and increased oxidation of long-chain fatty acids (bothcisandtransisomers)

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Kinetics Of The Enzymatic Transesterification Of Tuna Oil Catalyzed By Immobilized Candida Rugose Lipase To Produce Structured Lipid High In Omega-3 Fatty Acids

E3S Web of Conferences ◽

10.1051/e3sconf/20187306008 ◽

2018 ◽

Vol 73 ◽

pp. 06008 ◽

Cited By ~ 1

Author(s):

Amalia Rizka ◽

Wahyuningsih Wahyuningsih ◽

Broto RTD Wisnu ◽

Endy Yulianto Mohamad ◽

Rama Devara Hafizh ◽

...

Keyword(s):

Fatty Acids ◽

Fish Oil ◽

Lauric Acid ◽

Unsaturated Fatty Acids ◽

Structured Lipids ◽

Structured Lipid ◽

Omega 3 ◽

Enzymatic Transesterification ◽

Substrate Ratio ◽

Kinetics Of

Structured lipid containing Medium Chain of Fatty Acid (MCFA) at outer position and Poly-Unsaturated Fatty Acids (PUFA) at sn-2 position has nutritional value and excellent absorption. In this research, structured lipids was synthesized directly through enzymatic acidolysis between fish oil and lauric acid and catalyzed by specific lipase from immobilized 1.3 Candida rugose. The kinetics of enzymatic transesterification reactions catalyzed by immobilized Candida rugose was studied. To obtain the optimal condition, the factor substrate ratio of fish oil : lauric acid and reaction time were investigated. Simple mathematical model for DAG synthesis through transesterification mechanisms have been developed. The results showed that the parameters obtained had a good sensitivity. It was found that the kinetic model well describes the behavior of the reaction as the influence of the initial ratio of reactants.

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Effect of different forms of polyunsaturated fatty acids on duodenal and serum fatty acid profiles in sheep

Canadian Journal of Animal Science ◽

10.4141/cjas94-087 ◽

1994 ◽

Vol 74 (4) ◽

pp. 595-600 ◽

Cited By ~ 13

Author(s):

F. Enjalbert ◽

M. C. Nicot ◽

D. Griess ◽

M. Vernay ◽

R. Moncoulon

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Unsaturated Fatty Acids ◽

Vaccenic Acid ◽

Soy Oil ◽

Ruminal Fermentation ◽

Serum Fatty Acid ◽

Calcium Salts ◽

Physical Form ◽

Ruminal Biohydrogenation

Four sheep cannulated in the rumen and proximal duodenum were used in a 4 × 4 cross-over design to investigate the effects of ruminal fatty acid (FA) infusion on duodenal and serum FA profiles. The diets were composed of 85.7% natural grassland hay and 8.6% concentrate supplemented with 5.7% soy oil for diet SO, 5.7% emulsified soy oil for diet ESO, 6.7% calcium salts of soy or palm FA for diets CaSSO and CaSP, respectively. Diets were formulated to be isonitrogenous and isoenergetic; total FA content in dry matter was 6.4–6.6%. Characteristics of ruminal fermentation were not affected by source or physical form of FA. The proportion of stearic acid in the duodenal flow (% of the total C18) was high compared with total diet, e.g., 49.8 vs. 3.5 and 54.3 vs. 9.4% for soy and palm diets, respectively. Ruminal biohydrogenation and unsaturated FA was lower for CaS diets than for SO and ESO diets (48.7 and 60.9 vs. 81.2 and 94.7%, for oleic and linoleic acids, respectively). As a result, trans-vaccenic acid levels in duodenal flow and serum (% or total FA) were lower for the CaS diets than for SO and ESO diets (8.3 vs. 36.0% and 0.9 vs. 7.8%, respectively). Unsaturated FA as CaS were partly protected against ruminal biohydrogenation, and can be effective in increasing intestinal absorption of unsaturated FA. Key words: Unsaturated fatty acids, soy oil, calcium salts, biohydrogenation, sheep

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Effects of Fish Oil Sparing on Fillet Fatty Acid Composition in Hybrid Striped Bass Are Influenced by Dietary Levels of Saturated and Unsaturated Fatty Acids

North American Journal of Aquaculture ◽

10.1080/15222055.2014.963769 ◽

2015 ◽

Vol 77 (2) ◽

pp. 160-169 ◽

Cited By ~ 14

Author(s):

Jesse T. Trushenski ◽

Curtis C. Crouse ◽

Artur N. Rombenso

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Fish Oil ◽

Acid Composition ◽

Striped Bass ◽

Unsaturated Fatty Acids ◽

Hybrid Striped Bass

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Karakteristik dan Profil Asam Lemak Minyak Ikan dari Kepala dan Tulang Ikan Tuna (Thunnus albacares)

AGRITEKNO Jurnal Teknologi Pertanian ◽

10.30598/jagritekno.2020.9.1.10 ◽

2020 ◽

Vol 9 (1) ◽

pp. 10-19

Author(s):

Daniel A N Apituley ◽

Raja Bonan Dolok Sormin ◽

Esterlina E E M Nanlohy

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Fish Oil ◽

Iodine Number ◽

Fatty Acid Profile ◽

Unsaturated Fatty Acids ◽

Saturated Fatty Acids ◽

Monounsaturated Fatty Acids ◽

Acid Number ◽

Thunnus Albacares

This study was aimed to determine the physical and chemical quality as well as the fatty acid profile of fish oil from the waste of the head and the bones of Thunnus albacares. An experimental method was applied in this research. Observed variables included yield, density, acid number, saponification value, iodine number, TBA value, as well as fatty acid profile. The results showed that the physical characteristics of the oil from the head and bone of the fish, i.e., yield 12,11% and 9.85%, density 0.92 mg/mL, and 0.90 mg/mL, respectively. The chemical characteristics of the oil from head and bones of tuna were acid number 2.10 mg KOH/g and 2.88 mg KOH/g, iodine number 88.80 mg KOH/g and 77.67 mg KOH/g; saponification number 178.80 mg KOH/g and 145.50 mg KOH/g, TBA values 1.80 mg KOH/kg and 1.29 mg KOH/kg, subsequently. Unsaturated fatty acids were found to dominate oil from the head and bones of tuna. Tuna head contained 25 types of fatty acids consisting of 10 types of saturated fatty acids (SFA) 20.8% w/w, seven types of monounsaturated fatty acids (MUFA) 11.92% w/w, eight polyunsaturated fatty acids (PUFA) 35.98% w/w. In comparison, tuna bones contained 26 types of fatty acids consisting of 11 SFA 19.69% w/w, seven MUFA 10.80% w/w, and 8 PUFA 26.21% w/w. Keywords: fatty acid, fish oil, Thunnus albacares, waste of head and bone ABSTRAK Penelitian ini bertujuan untuk mengetahui kualitas fisik maupun kimiawi serta profil asam lemak minyak limbah ikan dari kepala dan tulang ikan tuna (Thunnus albacares). Metode yang digunakan adalah metode eksperimen. Parameter yang diamati yaitu: rendemen, berat jenis minyak, bilangan penyabunan, bilangan iodin, bilangan Tiobarbituric Acid (TBA) serta profil asam lemak. Hasil penelitian menunjukkan karakteristik fisik dari kepala dan tulang ikan berturut-turut adalah: rendemen 12,11 dan 9,85%; berat jenis minyak 0,92 mg/mL dan 0,90 mg/mL. Karakteristik kimia dari kepala dan tulang ikan tuna adalah berturut-turut: bilangan asam 2,10 mg KOH/g dan 2,88 mg KOH/g; bilangan iod 88,80 mg KOH/g dan 77,67 mg KOH/g; bilangan penyabunan 178,80 mg KOH/g dan 145,50 mg KOH/g; nilai TBA 1,80 mg KOH/kg dan 1,29 mg KOH/kg. Asam lemak tidak jenuh mendominasi minyak dari kepala maupun tulang ikan Tuna. Kepala ikan tuna mengandung 25 jenis asam lemak terdiri dari 10 jenis asam lemak jenuh (SFA) 20,8% w/w, 7 jenis lemak tak jenuh tunggal (MUFA) 11,92% w/w, 8 asam lemak tak jenuh jamak (PUFA) 35,98% w/w; sedangkan tulang ikan Tuna mengandung 26 jenis asam lemak terdiri dari 11 SFA 19,69% w/w, 7 MUFA 10,80% w/w, dan 8 PUFA 26,21% w/w. Kata kunci: asam lemak, minyak ikan, Thunnus albacares, limbah tulang dan kepala

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Characteristics of Pure Oils from Belly Fat (Pangasius hypophthalmus) with Bentonite Purification

Jurnal Pengolahan Hasil Perikanan Indonesia ◽

10.17844/jphpi.v21i3.24742 ◽

2018 ◽

Vol 21 (3) ◽

pp. 549 ◽

Cited By ~ 1

Author(s):

Lorde Sembiring ◽

Mirna Ilza ◽

Andarini Diharmi

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Fish Oil ◽

Peroxide Value ◽

Unsaturated Fatty Acids ◽

Essential Fatty Acids ◽

Purification Process ◽

Processing Industry ◽

Catfish Oil ◽

By Products

Catfish (Pangasius hypophthalmus) processing industry produces by products of abdoment fat containing unsaturated fatty acids, polyunsaturated fatty acids (PUFAs) which are the essential fatty acids needs to maintain health. Catfish belly fat can be processed into coarse fish oil through a purification process, with the addition of the adsorbent used, one of which is bentonite. This study was aimed to determine the<br />effect of bentonite application on the characteristics of refined catfish oil. Fish oil was extracted from belly fat then purified by adding bentonite adsorbent at a concentration of 1; 4 and 7%. The design used in this<br />study was a complete factorial of one factor. The parameters of analysis were the number of free fatty acids, peroxide value, anisidine, and totox. The results showed that bentonite had significant effect on free fatty<br />acid, peroxide, anisidine and totox values. The use of 7% bentonite decreased content of free fatty acid, peroxide value, anisidine, peroxide and totox from 1.72 to 0.85%, 5.18 to 0 meq/kg, 27.51 to 2.28 meq/kg,<br />and 37.88 to 2.28 meq/kg respectively.

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Effect of feeding diet containing fish oil and canola oil from transition period on milk production and components of Holstein cows in early lactation

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200029963 ◽

2009 ◽

Vol 2009 ◽

pp. 157-157

Author(s):

T Vafa ◽

A Naserian ◽

A Heravi Moussavi ◽

R Valizadeh ◽

M Danesh Mesgaran

Keyword(s):

Fatty Acids ◽

Fish Oil ◽

Dairy Cows ◽

Milk Production ◽

Canola Oil ◽

Unsaturated Fatty Acids ◽

Transition Period ◽

Holstein Cows ◽

Early Lactation ◽

Effect Of Feeding

There are too many physiological and nutritional factors which can influence lactation performance of early lactation Holstein dairy cows. Supplemental fat sources are utilized in rations for dairy cows as a common method to increase the energy density of the diet, especially in early lactation (Juchem et al., 2007). The fat sources also have a positive effect on milk fatty acid profile. Canola seed which contains 40% fat is an excellent source of dietary fat high in unsaturated fatty acids and protein for dairy animals. Canola oil contains 51% oleic, 25% linoleic, and 14% linolenic acids. Fish oil contains relatively high concentrations fatty acids of the n-3 family. It seems that feeding a blend of different oils will have more positive productive response than individual usage of them. The aim of this study was to evaluate the effect of feeding diets containing fish oil and canola oil starting from transition period on milk production and composition of early lactating Holstein cows.

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