Separation/Concentration-signal-amplification in-One Method Based on Electrochemical Conversion of Magnetic Nanoparticles for Electrochemical Biosensing

Amplified fluorescence target DNA detection was developed combining nicking endonuclease assisted target recycling and magnetic nanoparticles with low background signal.

Download Full-text

Nanomaterials for molecular signal amplification in electrochemical nucleic acid biosensing: recent advances and future prospects for point-of-care diagnostics

Molecular Systems Design & Engineering ◽

10.1039/c9me00135b ◽

2020 ◽

Vol 5 (1) ◽

pp. 49-66 ◽

Cited By ~ 11

Author(s):

Léonard Bezinge ◽

Akkapol Suea-Ngam ◽

Andrew J. deMello ◽

Chih-Jen Shih

Keyword(s):

Nucleic Acid ◽

Molecular Diagnostics ◽

Signal Amplification ◽

Point Of Care ◽

Future Prospects ◽

Electrochemical Biosensing ◽

Recent Advances ◽

Point Of Care Diagnostics ◽

Molecular Signal

This account reviews the major amplification strategies utilizing nanomaterials in electrochemical biosensing for robust and sensitive molecular diagnostics.

Download Full-text

DNA/RNA Electrochemical Biosensing Devices a Future Replacement of PCR Methods for a Fast Epidemic Containment

Sensors ◽

10.3390/s20164648 ◽

2020 ◽

Vol 20 (16) ◽

pp. 4648 ◽

Cited By ~ 3

Author(s):

Manikandan Santhanam ◽

Itay Algov ◽

Lital Alfonta

Keyword(s):

Signal Amplification ◽

Nucleic Acid Hybridization ◽

Diagnostic Tools ◽

Testing Method ◽

Reverse Transcription Pcr ◽

Electrochemical Biosensing ◽

Low Copy Number ◽

Rna Sensors ◽

Electrochemical Signal ◽

Sensitive Testing

Pandemics require a fast and immediate response to contain potential infectious carriers. In the recent 2020 Covid-19 worldwide pandemic, authorities all around the world have failed to identify potential carriers and contain it on time. Hence, a rapid and very sensitive testing method is required. Current diagnostic tools, reverse transcription PCR (RT-PCR) and real-time PCR (qPCR), have its pitfalls for quick pandemic containment such as the requirement for specialized professionals and instrumentation. Versatile electrochemical DNA/RNA sensors are a promising technological alternative for PCR based diagnosis. In an electrochemical DNA sensor, a nucleic acid hybridization event is converted into a quantifiable electrochemical signal. A critical challenge of electrochemical DNA sensors is sensitive detection of a low copy number of DNA/RNA in samples such as is the case for early onset of a disease. Signal amplification approaches are an important tool to overcome this sensitivity issue. In this review, the authors discuss the most recent signal amplification strategies employed in the electrochemical DNA/RNA diagnosis of pathogens.

Download Full-text

Nanobioconjugates for Signal Amplification in Electrochemical Biosensing

Molecules ◽

10.3390/molecules25153542 ◽

2020 ◽

Vol 25 (15) ◽

pp. 3542

Author(s):

Sebastian Cajigas ◽

Jahir Orozco

Keyword(s):

Quantum Dots ◽

Hybrid Materials ◽

Critical Review ◽

Signal Amplification ◽

Noble Metals ◽

Detection Limits ◽

Future Perspectives ◽

Electrochemical Biosensing ◽

Signal Response

Nanobioconjugates are hybrid materials that result from the coalescence of biomolecules and nanomaterials. They have emerged as a strategy to amplify the signal response in the biosensor field with the potential to enhance the sensitivity and detection limits of analytical assays. This critical review collects a myriad of strategies for the development of nanobioconjugates based on the conjugation of proteins, antibodies, carbohydrates, and DNA/RNA with noble metals, quantum dots, carbon- and magnetic-based nanomaterials, polymers, and complexes. It first discusses nanobioconjugates assembly and characterization to focus on the strategies to amplify a biorecognition event in biosensing, including molecular-, enzymatic-, and electroactive complex-based approaches. It provides some examples, current challenges, and future perspectives of nanobioconjugates for the amplification of signals in electrochemical biosensing.

Download Full-text