Variations in the expression of cell-adhesion molecules on liver-associated lymphocytes and peripheral-blood lymphocytes in patients with and without liver metastasis

1995 ◽  
Vol 61 (4) ◽  
pp. 475-479 ◽  
Author(s):  
María García-Barcina ◽  
Ioseba Bidaurrazaga ◽  
Véronique Neaud ◽  
Paulette Bioulac-Sage ◽  
Charles Balabaud ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Liver Metastasis ◽  
Adhesion Molecules ◽  
Peripheral Blood ◽  
Cell Adhesion Molecules ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes

Modification of Cytokine Levels and CD34+ Stem Cell Adhesion Molecules during Mobilization in Multiple Myeloma (MM) Patients.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 5068-5068
Author(s):  
Patrizia Zappasodi ◽  
Alessandro Corso ◽  
Chiara Rusconi ◽  
Mara De Amici ◽  
Erica Consensi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Peripheral Blood ◽  
Cell Adhesion Molecules ◽  
Serum Levels ◽  
High Baseline ◽  
Cytokine Levels ◽  
Baseline Levels

Abstract The mechanisms underlying the mobilization of peripheral blood stem cells (PBSC) are still unknown, even though studies on healthy donors and on cancer patients have hypothesised a central role for CD34+ stem cells adhesion molecules and for chemokines serum levels. The type of mobilizing regimen and the pathologic condition can exert different effects on cytokine levels and adhesion molecules expression. To evaluate the modification of these parameters during mobilization we studied 10 MM patients treated with DCEP chemotherapy (Decadron, Cyclophosphamide, Etoposide, cis-Platin) followed by G-CSF (5 mg/kg/day starting 48 hours after chemotherapy until PBSC collection). The expression of four CD34+ cell adhesion molecules (Thy1, L-selectin, VLA4, CXCR4) was measured in the bone marrow before mobilization therapy and in the leukapheresis product. Serum levels of five cytokines (TGF-β, IL-8, HGF, VEGF-A, sVCAM-1) were assessed, through specific ELISA kits, before therapy, 5 days after the G-CSF stimulation and at stem cells collection. Each parameter was correlated with the number of CD34+ cells collected. Thy1 significantly decreased (p=0.007) and L-selectin significantly increased (p=0.038) in nearly all cases. By contrast, no statistically significant differences were observed for VLA4 and CXCR4 due to a consistent variability. Serum levels of IL-8 showed an increase at day 5, then a significant decrease at the time of the harvest (p=0.013); VEGF-A constantly increased (p<0.001) and TGF-β decreased (p=0.001). The modifications registered for sVCAM-1 and HGF did not reach statistical significance. The expression of CD34+ adhesion molecules did not correlate with the number of PBSC collected, while among cytokines, high baseline levels of TGF-β and VEGF-A significantly correlated with high number of CD34+ stem cells yielded (p=0.016, p=0.042 respectively). In conclusion, this study shows that during stem cells mobilization in MM Thy-1 expression significantly decreases while L-selectin increases; the behaviour of VLA-4 and CXCR4 is extremely variable. As regards cytokines, we found a statistically significant increase of VEGF-A level, while TGF-β, which induces the stem cell homing, decreases during the mobilization allowing stem cells to migrate in the peripheral blood. High baseline levels of TGF-β and VEGF-A predict a good stem cells harvest.

scholarly journals PO-050 Effects of Exercise on Gene Expression Correlative to Bone Metabolism in Peripheral Blood Mononuclear Cells

2018 ◽  
Vol 1 (3) ◽  
Author(s):  
Yongjie Yang ◽  
Li Gao
Keyword(s):  
Gene Expression ◽  
Cell Adhesion ◽  
Bone Metabolism ◽  
Adhesion Molecules ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Cell Adhesion Molecules ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Objective To examine the gene expression pattern of Peripheral Blood Mononuclear Cells （PBMCs）and to explore exercise-sensitive genes correlative to bone metabolism in PBMCs. Thus to provide a theoretical basis for exercise intervention to prevent and treat osteoporosis.  Methods Uphill (+8° ) and downhill (-8° ) training were used for the exercise loading in two-month-old male Sprague-Dawley rats. The exercise method performed at 25 m/min, training 50 min/d, 5 d/wk, for 12 wk, respectively. Bone mineral density of distal femurs was measured using dual X-ray absorptiometry, and the expressed gene profile of PBMCs was examined using Gene Chip IVT Labeling Kit (Affymetrix).  Results Compared with control (CON) group, the BMD of the femur in the downhill (DOWN) group was significantly increased. Compared with the uphill （UP）group, the BMD and BMC of the femur in the DOWN group were significantly increased. There were 38 genes detected differentially expressed between two exercise groups together with CON group. The expression of genes modified by running involved in immunity, cell proliferation，Rheumatoid arthritis，Cell adhesion molecules and Tnf signaling pathway. There were 105 differently expressed genes between the DOWN group and the UP group which were mainly enriched in biological processes and pathways such as response to hydrogen peroxide，lipopolysaccharide，cell factor and  mechanical stimulus，Cell adhesion molecules，cell migration，collagen biosynthetic process and Tnf signaling pathway. Tnf, Cxcl2, Ccl2, Jun and Mmp9 as the key nodes of protein interaction network were identified as candidate genes related to bone metabolism and sensitive to exercise.  Conclusions With weight gaining，age increasing and training time prolonging, long-term and high-intensity exercise will be harm for bone. At the same time and same running speed, downhill running conduces to increase bone density more than uphill running. It may be associated with differential expression of exercise-sensitive genes involved in bone metabolism in PBMCs.

The Higher CD34+ Cell Counts in Cord Blood Than Those in Neonatal Peripheral Blood Correlate with Increased Expression of Cytokine Receptors and Cell Adhesion Molecules on the CD34+ Cells in Cord Blood.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4126-4126
Author(s):  
Young-Ho Lee ◽  
Joong-Pyo Kim ◽  
Myung-Hwa Sung ◽  
Ri-Young Ko ◽  
Jin-Yeong Han
Keyword(s):  
Cell Adhesion ◽  
Cord Blood ◽  
Adhesion Molecules ◽  
Peripheral Blood ◽  
Cell Adhesion Molecules ◽  
Mononuclear Cells ◽  
Cytokine Receptors ◽  
Hematopoietic Stem ◽  
Cell Counts ◽  
Cd34 Cells

Abstract The hematologic values of neonatal peripheral blood show a characteristic series of changes during neonatal period. The hematopoietic stem cells in cord blood (CB) are more primitive and abundant than those in neonatal peripheral blood (NB). However, there have been no studies regarding the reasons for those differences. Thus, We investigated the kinetics of nucleated cells and CD34+ cells in CB and neonatal peripheral blood. We obtained the CB and NB samples from 14 normal full-term babies for this study, which were collected immediately after delivery as well as at 24 hours and 48 hours after delivery. We analysed the expressions of CD34, CD34/CXCR4, CD34/CD49d and CD34/CD44 as well as CD3, CD19 and CD16/56 on the isolated mononuclear cells by using FACSort. We also performed CFU-GM counts and apoptotic analysis with isolated mononuclear cells. The total white blood cell counts as well as absolute neutrophil counts of NB at delivery were higher than those of CB (p=0.04) and increased until 24 hours, when decreased until 48 hours after delivery. There were no differences of absolute lymphocyte counts, CD3+ cell and CD19+ cell counts among CB and NB at 0, 24 and 48 hours postnatally, however, the CD16/56+ cell counts were lower in CB than in NB and gradually decreased until 48 hours after delivery (p=0.011). The number of CFU-GM counts (p=0.02) and CD34+ cells (p=0.049) as well as CD34+CXCR4+ cells (p=0.002), CD34+CD49d+ (p=0.001) and CD34+CD44+ cells (p=0.001) in CB were more significantly higher than those of NB at delivery, when decreased gradually until 48 hours after delivery. The incidence of apoptosis were statistically not significant among CB and NB at 0, 24 and 48 hours postnatally. In conclusion, the higher leukocyte counts for several days after delivery were correlated with neutrophilic increment which may be influenced by stressful events during delivery. The more higher CFU-GM counts and CD34+ cell counts in CB than those in NB might be correlated with increased expression of cytokine receptors and cell adhesion molecules on the CD34+ cells in CB.

scholarly journals Activation and increased expression of adhesion molecules on peripheral blood lymphocytes is a mechanism for the immediate lymphocytopenia after administration of OKT3

Blood ◽  
1996 ◽  
Vol 87 (1) ◽  
pp. 404-411 ◽  
Author(s):  
S Buysmann ◽  
FJ Bemelman ◽  
PT Schellekens ◽  
Y van Kooyk ◽  
CG Figdor ◽  
...  
Keyword(s):  
T Cells ◽  
Monoclonal Antibodies ◽  
T Lymphocytes ◽  
Adhesion Molecules ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Intercellular Adhesion Molecule ◽  
Intercellular Adhesion Molecule 1 ◽  
Blood Lymphocytes

Abstract We investigated the mechanism by which antihuman CD3 monoclonal antibodies of the isotypes IgG2a (eg, OKT3) and IgA (eg, IXA) can induce the rapid disappearance of virtually all circulating T lymphocytes. We hypothesize that upregulation of adhesion molecules on the lymphocyte membrane contributes to this effect. However, this hypothesis is difficult to test, because of the inherent lymphocytopenia and/or shifts in lymphocyte populations between intra and extra-vascular compartments. Therefore, studies in vitro were performed, as well. Analysis of peripheral blood lymphocytes isolated at several times after addition of OKT3 or IXA to whole blood of healthy individuals showed an immediate increase in the proportion of T cells expressing NKI-L16, an activation epitope on CD11a/CD18. Likewise, an increase in CD11b/CD18 expression occurred. In parallel experiments, a transiently increased adhesion of T cells to endothelial cell monolayers was observed. This adhesion could be completely blocked by anti-CD18 or anti-CD11a monoclonal antibodies and only partly by an anti-CD11b antibody. Our data indicate that upregulation of activation epitopes of CD11a/CD18, as well as increased expression of CD11b/CD18 on T lymphocytes, may result in increased adhesion of these cells to intercellular adhesion molecule-1 (ICAM-1) and ICAM-2 on vascular endothelium. This phenomenon may, at least, partly explain the rapidly occurring peripheral lymphocytopenia observed in vivo.

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