scholarly journals A new type of signal peptide: central role of a twin-arginine motif in transfer signals for the delta pH-dependent thylakoidal protein translocase.

1995 ◽  
Vol 14 (12) ◽  
pp. 2715-2722 ◽  
Author(s):  
A.M. Chaddock ◽  
A. Mant ◽  
I. Karnauchov ◽  
S. Brink ◽  
R.G. Herrmann ◽  
...  
Keyword(s):  
Signal Peptide ◽  
New Type ◽  
Ph Dependent

scholarly journals Role of the Stable Signal Peptide of Junín Arenavirus Envelope Glycoprotein in pH-Dependent Membrane Fusion

2006 ◽  
Vol 80 (15) ◽  
pp. 7775-7780 ◽  
Author(s):  
Joanne York ◽  
Jack H. Nunberg
Keyword(s):  
Amino Acid ◽  
Membrane Fusion ◽  
Signal Peptide ◽  
Envelope Glycoprotein ◽  
Amino Acid Signal Peptide ◽  
Ph Dependent ◽  
Mediate Cell ◽  
C Complex ◽  
Stable Signal

ABSTRACT The envelope glycoprotein of the arenaviruses (GP-C) is unusual in that the mature complex retains the cleaved, 58-amino-acid signal peptide. Association of this stable signal peptide (SSP) has been shown to be essential for intracellular trafficking and proteolytic maturation of the GP-C complex. We identify here a specific and previously unrecognized role of SSP in pH-dependent membrane fusion. Amino acid substitutions that alter the positive charge at lysine K33 in SSP affect the ability of GP-C to mediate cell-cell fusion and the threshold pH at which membrane fusion is triggered. Based on the presumed location of K33 at or near the luminal domain of SSP, we postulate that SSP interacts with the membrane-proximal or transmembrane regions of the G2 fusion protein. This unique organization of the GP-C complex may suggest novel strategies for intervention in arenavirus infection.

Toward a theory on workplaces for smart workers

Facilities ◽  
2019 ◽  
Vol 38 (3/4) ◽  
pp. 298-315
Author(s):  
Luisa Errichiello ◽  
Tommasina Pianese
Keyword(s):  
Web Sites ◽  
Participant Observation ◽  
Secondary Data ◽  
Primary Data ◽  
Innovative Design ◽  
Content Type ◽  
New Type ◽  
Smart Working ◽  
Research Propositions

Purpose The purpose of this paper is to identify the main features of smart work centers (SWCs) and show how these innovative offices would support the implementation of smart working and related changes in workspaces (“bricks”), technologies (“bytes”) and organizational practices (“behaviors”). Design/methodology/approach In this study, scientific literature is combined with white papers and business reports and visits to 14 workplaces, including offices designed as SWCs, co-working spaces, one telecenter, one accelerator and one fab lab. Primary data were collected through interviews with managers and users and non-participant observation, whereas secondary data included web-sites, brochures, presentations, press releases and official documents. Findings The authors developed research propositions about how the design of spaces and the availability of technology within SWCs would support the “bricks” and “bytes” levers of smart working. More importantly, the authors assumed that this new type of workplace would sustain changes in employees’ behaviors and managers’ practices, thus helping to overcome several challenges traditionally associated with remote working. Research limitations/implications The exploratory nature of the research only provides preliminary information about the role of SWCs within smart working programs. Additional qualitative and quantitative empirical investigation is required. Practical implications This study provides valuable knowledge about how the design of corporate offices can be leveraged to sustain the implementation of smart working. Originality/value This study advances knowledge on workplaces by focusing on an innovative design of traditional offices (SWC). It also lays the foundations for future investigation aimed at testing the developed propositions.

The Entrepreneurial University: Evidence of the Changing Role of Universities in Modern Russia

2014 ◽  
Vol 28 (4) ◽  
pp. 271-280 ◽  
Author(s):  
Dina Williams ◽  
Alexey Kluev
Keyword(s):  
Entrepreneurial University ◽  
Primary Data ◽  
Strategy Development ◽  
Infrastructure Development ◽  
Innovation Development ◽  
Policy Makers ◽  
Managerial Competencies ◽  
New Type ◽  
Changing Role

This paper examines the evolution of the entrepreneurial function of Russia's leading universities: the study is based on a conceptualization of a university's entrepreneurial development pathway developed by the OECD in 2009. The data on entrepreneurship development were collected in 2012 through a survey of technology transfer and innovation development managers of 18 National Research Universities. The primary data were complemented by desktop analysis of the strategy development documents of the universities surveyed. The results suggest that in the period studied, 2008–2012, a new type of university, the entrepreneurial university, has emerged in Russia. However, these entrepreneurial universities are facing serious challenges in the implementation of an entrepreneurial, innovation-oriented mission due to the lack of managerial competencies and inadequate infrastructure development. The paper has significant theoretical and practical implications in shedding light on the development of the entrepreneurial university in Russia, where the process is still in its infancy. The authors depict a trajectory of entrepreneurial transformation in and barriers to this process that, it is argued, university executives and policy makers should take into consideration.

scholarly journals Protein Engineering Approaches to Enhance Fungal Laccase Production in S. cerevisiae

2021 ◽  
Vol 22 (3) ◽  
pp. 1157
Author(s):  
Pablo Aza ◽  
Felipe de Salas ◽  
Gonzalo Molpeceres ◽  
David Rodríguez-Escribano ◽  
Iñigo de la Fuente ◽  
...  
Keyword(s):  
Protein Engineering ◽  
Directed Evolution ◽  
Signal Peptide ◽  
Laccase Activity ◽  
Laccase Production ◽  
Wide Range ◽  
Mating Factor ◽  
Conserved Amino Acids ◽  
Basidiomycete Fungi

Laccases secreted by saprotrophic basidiomycete fungi are versatile biocatalysts able to oxidize a wide range of aromatic compounds using oxygen as the sole requirement. Saccharomyces cerevisiae is a preferred host for engineering fungal laccases. To assist the difficult secretion of active enzymes by yeast, the native signal peptide is usually replaced by the preproleader of S. cerevisiae alfa mating factor (MFα1). However, in most cases, only basal enzyme levels are obtained. During directed evolution in S. cerevisiae of laccases fused to the α-factor preproleader, we demonstrated that mutations accumulated in the signal peptide notably raised enzyme secretion. Here we describe different protein engineering approaches carried out to enhance the laccase activity detected in the liquid extracts of S. cerevisiae cultures. We demonstrate the improved secretion of native and engineered laccases by using the fittest mutated α-factor preproleader obtained through successive laccase evolution campaigns in our lab. Special attention is also paid to the role of protein N-glycosylation in laccase production and properties, and to the introduction of conserved amino acids through consensus design enabling the expression of certain laccases otherwise not produced by the yeast. Finally, we revise the contribution of mutations accumulated in laccase coding sequence (CDS) during previous directed evolution campaigns that facilitate enzyme production.

Some Origins of Nationalism in East Africa

1968 ◽  
Vol 9 (1) ◽  
pp. 119-146 ◽  
Author(s):  
J. M. Lonsdale
Keyword(s):  
Central Government ◽  
Colonial Period ◽  
Political Activity ◽  
Colonial Rule ◽  
Face To Face ◽  
National Movements ◽  
New Type ◽  
First Time ◽  
Official Recognition

This paper attempts to provide a frame of reference for evaluating the role of ordinary rural Africans in national movements, in the belief that scholarly preoccupation with élites will only partially illumine the mainsprings of nationalism. Kenya has been taken as the main field of enquiry, with contrasts and comparisons drawn from Uganda and Tanganyika. The processes of social change are discussed with a view to establishing that by the end of the colonial period one can talk of peasants rather than tribesmen in some of the more progressive areas. This change entailed a decline in the leadership functions of tribal chiefs who were also the official agents of colonial rule, but did not necessarily mean the firm establishment of a new type of rural leadership. The central part of the paper is taken up with an account of the competition between these older and newer leaderships, for official recognition rather than a mass following. A popular following was one of the conditions for such recognition, but neither really achieved this prior to 1945 except in Kikuyuland, and there the newer leaders did not want official recognition. After 1945 the newer leadership, comprising especially traders and officials of marketing co-operatives, seems everywhere to have won a properly representative position, due mainly to the enforced agrarian changes which brought the peasant face to face with the central government, perhaps for the first time. This confrontation, together with the experience of failure in earlier and more local political activity, resulted in a national revolution coalescing from below, co-ordinated rather than instigated by the educated élite.

pH‐Dependent Degradation of Layered Black Phosphorus: Essential Role of Hydroxide Ions

2018 ◽  
Author(s):  
Siyu Zhang ◽  
Xuejiao Zhang ◽  
Lei Lei ◽  
Xue‐Feng Yu ◽  
Jingwen Chen ◽  
...  
Keyword(s):  
Essential Role ◽  
Black Phosphorus ◽  
Ph Dependent

scholarly journals THE ROLE OF MALAY TRADITIONAL HOUSE AND RUMAH GADANG AS HOMESTAYS

2021 ◽  
Vol 8 (1) ◽  
pp. 125
Author(s):  
Rohaslinda Ramele ◽  
Jonny Wongso
Keyword(s):  
Rural Tourism ◽  
West Sumatra ◽  
New Type

In both Malaysia and Indonesia, traditional houses have been used as homestays, as a new type of rural tourism accommodation. This study aims to compare the implementation of rural tourism in Malaysia and Indonesia, to reveal the usage of traditional Malay house and Rumah Gadang as homestays in both countries, and to promote homestay as an alternative for rural tourism accommodation. Experiences on participating in both homestays in Kampung Lonek Homestay, Negeri Sembilan, Malaysia and Nagari Sumpur, West Sumatra, both majorly populated by the Minang community, are shared and analyzed to achieve these aims.   

scholarly journals Authorial Self and Modernity as Reflected in Diaries and Memoirs. Three 19th-Century Hungarian Case Studies

2019 ◽  
Vol 9 (1) ◽  
pp. 85-97
Author(s):  
Béla Mester
Keyword(s):  
Intellectual History ◽  
Social Role ◽  
Public Intellectual ◽  
Self Reflection ◽  
Individual Character ◽  
History Of ◽  
New Type ◽  
The Moment ◽  
The One

Abstract The role of the diaries and memoirs in the process of the conscious self-reflection and their contribution to the emergence of modern individual personalities are well-known facts of the intellectual history. The present paper intends to analyze a special form of the creation of modern individual character; it is the self-creation of the writer as a conscious personality, often with a clearly formulated opinion about her/his own social role. There will be offered several examples from the 19th-century history of the Hungarian intelligentsia. This period is more or less identical with the modernization of the “cultural industry” in Hungary, dominated by the periodicals with their deadlines, fixed lengths of the articles, and professional editing houses on the one hand and the cultural nation building on the other. Concerning the possible social and cultural role of the intelligentsia, it is the moment of the birth of a new type, so-called public intellectual. I will focus on three written sources, a diary of a Calvinist student of theology, Péter (Litkei) Tóth, the memoirs of an influential public intellectual, Gusztáv Szontagh, and a belletristic printed diary of a young intellectual, János Asbóth.

scholarly journals Language in social reproduction: Sociolinguistics and sociosemiotics

2009 ◽  
Vol 37 (1/2) ◽  
pp. 43-81
Author(s):  
Patrizia Calefato
Keyword(s):  
Social Reproduction ◽  
Social Dimension ◽  
The Social ◽  
The Sixties ◽  
Roman Jakobson ◽  
New Type ◽  
General Linguistics ◽  
Definition Of ◽  
The Relationship

This paper focuses on the semiotic foundations of sociolinguistics. Starting from the definition of “sociolinguistics” given by the philosopher Adam Schaff, the paper examines in particular the notion of “critical sociolinguistics” as theorized by the Italian semiotician Ferruccio Rossi-Landi. The basis of the social dimension of language are to be found in what Rossi-Landi calls “social reproduction” which regards both verbal and non-verbal signs. Saussure’s notion of langue can be considered in this way, with reference not only to his Course of General Linguistics, but also to his Harvard Manuscripts.The paper goes on trying also to understand Roland Barthes’s provocative definition of semiology as a part of linguistics (and not vice-versa) as well as developing the notion of communication-production in this perspective. Some articles of Roman Jakobson of the sixties allow us to reflect in a manner which we now call “socio-semiotic” on the processes of transformation of the “organic” signs into signs of a new type, which articulate the relationship between organic and instrumental. In this sense, socio-linguistics is intended as being sociosemiotics, without prejudice to the fact that the reference area must be human, since semiotics also has the prerogative of referring to the world of non-human vital signs.Socio-linguistics as socio-semiotics assumes the role of a “frontier” science, in the dual sense that it is not only on the border between science of language and the anthropological and social sciences, but also that it can be constructed in a movement of continual “crossing frontiers” and of “contamination” between languages and disciplinary environments.

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