Responses of human keratocytes to micro- and nanostructured substrates

2004 ◽  
Vol 71A (3) ◽  
pp. 369-376 ◽  
Author(s):  
Ana I. Teixeira ◽  
Paul F. Nealey ◽  
Christopher J. Murphy
Keyword(s):  
Nanostructured Substrates ◽  
Human Keratocytes

The effect of dichlorotriazinyl aminofluorescein on human keratocytes in vitro

1996 ◽  
Vol 10 (2) ◽  
pp. 63 ◽  
Author(s):  
Won Ryang Wee ◽  
Roya Rofougaran ◽  
Alireza Pakkar ◽  
Seiji Hayashi ◽  
Peter J. McDonnell
Keyword(s):  
Human Keratocytes

Formation of surface nanobubbles on nanostructured substrates

Nanoscale ◽  
2017 ◽  
Vol 9 (3) ◽  
pp. 1078-1086 ◽  
Author(s):  
Lei Wang ◽  
Xingya Wang ◽  
Liansheng Wang ◽  
Jun Hu ◽  
Chun Lei Wang ◽  
...  
Keyword(s):  
Nanostructured Substrates

scholarly journals Expression Profiles of Neuropeptides, Neurotransmitters, and Their Receptors in Human Keratocytes In Vitro and In Situ

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0134157 ◽  
Author(s):  
Marta Słoniecka ◽  
Sandrine Le Roux ◽  
Peter Boman ◽  
Berit Byström ◽  
Qingjun Zhou ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Human Keratocytes

Interfacial charge transfer events of BODIPY molecules: single molecule spectroelectrochemistry and substrate effects

2014 ◽  
Vol 16 (42) ◽  
pp. 23150-23156 ◽  
Author(s):  
Jia Liu ◽  
Caleb M. Hill ◽  
Shanlin Pan ◽  
Haiying Liu
Keyword(s):  
Charge Transfer ◽  
Single Molecule ◽  
Single Molecules ◽  
Transfer Mechanism ◽  
Substrate Effects ◽  
Interfacial Charge Transfer ◽  
Charge Transfer Mechanism ◽  
Nanostructured Substrates ◽  
Interfacial Charge

BODIPY dye single molecules on nanostructured substrates are studied with a single molecule spectroelectrochemistry technique to reveal the heterogeneous charge transfer mechanism.

scholarly journals Single-cell analysis reveals IGF-1 potentiation of inhibition of the TGF-β/Smad pathway of fibrosis in human keratocytes in vitro

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Tomislav Sarenac ◽  
Martin Trapecar ◽  
Lidija Gradisnik ◽  
Marjan Slak Rupnik ◽  
Dusica Pahor
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Smad Pathway ◽  
Human Keratocytes

scholarly journals Cell signaling pathways in human mutant PAX6 corneal cells: an in vitro model for aniridia-related keratopathy

2021 ◽  
Author(s):  
Marta Słoniecka ◽  
André Vicente ◽  
Berit Byström ◽  
Fátima Pedrosa Domellöf
Keyword(s):  
Protein Expression ◽  
Signaling Pathways ◽  
Cell Fate ◽  
Expression Patterns ◽  
Pax6 Gene ◽  
Cas9 Nuclease ◽  
Extracellular Matrix Components ◽  
Gene And Protein Expression ◽  
Human Keratocytes

ABSTRACTPURPOSETo establish an in vitro model of aniridia-related keratopathy (ARK) using CRISPR/Cas9 engineered human keratocytes with mutations in the PAX6 gene, and to study the Notch Homolog 1, Translocation-Associated (Notch1), sonic hedgehog (SHH), mammalian target of rapamycin (mTOR), and Wnt/β-catenin signaling pathways in the PAX6 mutant keratocytes.METHODSPrimary human keratocytes were isolated from healthy corneas. Keratocytes were transduced with Cas9 lentiviral particles in order to create cells stably expressing Cas9 nuclease. Lentiviral particles carrying PAX6 sgRNA were transduced into the Cas9 keratocytes creating mutants. Analysis of signaling pathways was assessed by RT-qPCR for gene expression and western blot for protein expression.RESULTSHuman keratocytes stably expressing Cas9 nuclease were created. Keratocytes carrying PAX6 gene mutation were successfully generated. PAX6 mutant keratocytes showed modified expression patterns of extracellular matrix components such as collagens and fibrotic markers. Analysis of the Notch1, SHH, mTOR, and Wnt/β-catenin signaling pathways in the PAX6 mutant keratocytes revealed altered gene and protein expression of the key players involved in these pathways.CONCLUSIONSA properly functioning PAX6 gene in keratocytes is crucial for the regulation of signaling pathways important for cell fate determination, proliferation, and inflammation. Pax6 mutation in the in vitro settings leads to changes in these pathways which resemble those found in corneas of patients with ARK.

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