Responses of human keratocytes to micro- and nanostructured substrates

2004 ◽  
Vol 71A (3) ◽  
pp. 369-376 ◽  
Author(s):  
Ana I. Teixeira ◽  
Paul F. Nealey ◽  
Christopher J. Murphy
1996 ◽  
Vol 10 (2) ◽  
pp. 63 ◽  
Author(s):  
Won Ryang Wee ◽  
Roya Rofougaran ◽  
Alireza Pakkar ◽  
Seiji Hayashi ◽  
Peter J. McDonnell
Keyword(s):  

Nanoscale ◽  
2017 ◽  
Vol 9 (3) ◽  
pp. 1078-1086 ◽  
Author(s):  
Lei Wang ◽  
Xingya Wang ◽  
Liansheng Wang ◽  
Jun Hu ◽  
Chun Lei Wang ◽  
...  

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0134157 ◽  
Author(s):  
Marta Słoniecka ◽  
Sandrine Le Roux ◽  
Peter Boman ◽  
Berit Byström ◽  
Qingjun Zhou ◽  
...  

2014 ◽  
Vol 16 (42) ◽  
pp. 23150-23156 ◽  
Author(s):  
Jia Liu ◽  
Caleb M. Hill ◽  
Shanlin Pan ◽  
Haiying Liu

BODIPY dye single molecules on nanostructured substrates are studied with a single molecule spectroelectrochemistry technique to reveal the heterogeneous charge transfer mechanism.


2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Tomislav Sarenac ◽  
Martin Trapecar ◽  
Lidija Gradisnik ◽  
Marjan Slak Rupnik ◽  
Dusica Pahor

2021 ◽  
Author(s):  
Marta Słoniecka ◽  
André Vicente ◽  
Berit Byström ◽  
Fátima Pedrosa Domellöf

ABSTRACTPURPOSETo establish an in vitro model of aniridia-related keratopathy (ARK) using CRISPR/Cas9 engineered human keratocytes with mutations in the PAX6 gene, and to study the Notch Homolog 1, Translocation-Associated (Notch1), sonic hedgehog (SHH), mammalian target of rapamycin (mTOR), and Wnt/β-catenin signaling pathways in the PAX6 mutant keratocytes.METHODSPrimary human keratocytes were isolated from healthy corneas. Keratocytes were transduced with Cas9 lentiviral particles in order to create cells stably expressing Cas9 nuclease. Lentiviral particles carrying PAX6 sgRNA were transduced into the Cas9 keratocytes creating mutants. Analysis of signaling pathways was assessed by RT-qPCR for gene expression and western blot for protein expression.RESULTSHuman keratocytes stably expressing Cas9 nuclease were created. Keratocytes carrying PAX6 gene mutation were successfully generated. PAX6 mutant keratocytes showed modified expression patterns of extracellular matrix components such as collagens and fibrotic markers. Analysis of the Notch1, SHH, mTOR, and Wnt/β-catenin signaling pathways in the PAX6 mutant keratocytes revealed altered gene and protein expression of the key players involved in these pathways.CONCLUSIONSA properly functioning PAX6 gene in keratocytes is crucial for the regulation of signaling pathways important for cell fate determination, proliferation, and inflammation. Pax6 mutation in the in vitro settings leads to changes in these pathways which resemble those found in corneas of patients with ARK.


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