Peptide-modified methacrylated glycol chitosan hydrogels as a cell-viability supporting pro-angiogenic cell delivery platform for human adipose-derived stem/stromal cells

2018 ◽  
Vol 107 (3) ◽  
pp. 571-585 ◽  
Author(s):  
Jobanpreet Dhillon ◽  
Stuart A. Young ◽  
Stephen E. Sherman ◽  
Gillian I. Bell ◽  
Brian G. Amsden ◽  
...  
Keyword(s):  
Cell Viability ◽  
Stromal Cells ◽  
Cell Delivery ◽  
Glycol Chitosan ◽  
Delivery Platform ◽  
A Cell ◽  
Chitosan Hydrogels

scholarly journals Nanofibrillar cellulose wound dressing supports the growth and characteristics of human mesenchymal stem/stromal cells without cell adhesion coatings

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Jasmi Kiiskinen ◽  
Arto Merivaara ◽  
Tiina Hakkarainen ◽  
Minna Kääriäinen ◽  
Susanna Miettinen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Adhesion ◽  
Cell Viability ◽  
Cell Survival ◽  
Cell Transplantation ◽  
Stromal Cells ◽  
Wound Dressing ◽  
Nanofibrillar Cellulose ◽  
Cell Scaffold ◽  
A Cell

Abstract Background In the field of regenerative medicine, delivery of human adipose-derived mesenchymal stem/stromal cells (hASCs) has shown great promise to promote wound healing. However, a hostile environment of the injured tissue has shown considerably to limit the survival rate of the transplanted cells, and thus, to improve the cell survival and retention towards successful cell transplantation, an optimal cell scaffold is required. The objective of this study was to evaluate the potential use of wood-derived nanofibrillar cellulose (NFC) wound dressing as a cell scaffold material for hASCs in order to develop a cell transplantation method free from animal-derived components for wound treatment. Methods Patient-derived hASCs were cultured on NFC wound dressing without cell adhesion coatings. Cell characteristics, including cell viability, morphology, cytoskeletal structure, proliferation potency, and mesenchymal cell and differentiation marker expression, were analyzed using cell viability assays, electron microscopy, immunocytochemistry, and quantitative or reverse transcriptase PCR. Student’s t test and one-way ANOVA followed by a Tukey honestly significant difference post hoc test were used to determine statistical significance. Results hASCs were able to adhere to NFC dressing and maintained high cell survival without cell adhesion coatings with a cell density-dependent manner for the studied period of 2 weeks. In addition, NFC dressing did not induce any remarkable cytotoxicity towards hASCs or alter the morphology, proliferation potency, filamentous actin structure, the expression of mesenchymal vimentin and extracellular matrix (ECM) proteins collagen I and fibronectin, or the undifferentiated state of hASCs. Conclusions As a result, NFC wound dressing offers a functional cell culture platform for hASCs to be used further for in vivo wound healing studies in the future.

scholarly journals Supercharged ferritin as a cell delivery platform

2013 ◽  
Vol 69 (a1) ◽  
pp. s331-s331
Author(s):  
Tobias Beck ◽  
Matthias Kuenzle ◽  
Donald Hilvert
Keyword(s):  
Cell Delivery ◽  
Delivery Platform ◽  
A Cell

Targeted gene addition to human mesenchymal stromal cells as a cell-based plasma-soluble protein delivery platform

Cytotherapy ◽  
2010 ◽  
Vol 12 (3) ◽  
pp. 394-399 ◽  
Author(s):  
Basma F. Benabdallah ◽  
Emilie Allard ◽  
Shuyuan Yao ◽  
Geoffrey Friedman ◽  
Philip D. Gregory ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Soluble Protein ◽  
Protein Delivery ◽  
Human Mesenchymal Stromal Cells ◽  
Delivery Platform ◽  
A Cell

scholarly journals Composite scaffold provides a cell delivery platform for cardiovascular repair

2011 ◽  
Vol 108 (19) ◽  
pp. 7974-7979 ◽  
Author(s):  
A. F. G. Godier-Furnemont ◽  
T. P. Martens ◽  
M. S. Koeckert ◽  
L. Wan ◽  
J. Parks ◽  
...  
Keyword(s):  
Composite Scaffold ◽  
Cell Delivery ◽  
Delivery Platform ◽  
A Cell

Effects of hypoxia on osteogenic differentiation of mesenchymal stromal cells used as a cell therapy for avascular necrosis of the femoral head

Cytotherapy ◽  
2016 ◽  
Vol 18 (9) ◽  
pp. 1087-1099 ◽  
Author(s):  
Gabriela Ciapetti ◽  
Donatella Granchi ◽  
Caterina Fotia ◽  
Lucia Savarino ◽  
Dante Dallari ◽  
...  
Keyword(s):  
Femoral Head ◽  
Cell Therapy ◽  
Osteogenic Differentiation ◽  
Avascular Necrosis ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
A Cell

scholarly journals Echocardiography-guided percutaneous left ventricular intracavitary injection as a cell delivery approach in infarcted mice

2021 ◽  
Vol 476 (5) ◽  
pp. 2135-2148
Author(s):  
Yibing Nong ◽  
Yiru Guo ◽  
Alex Tomlin ◽  
Xiaoping Zhu ◽  
Marcin Wysoczynski ◽  
...  
Keyword(s):  
Left Ventricular ◽  
Cell Delivery ◽  
A Cell ◽  
Delivery Approach

scholarly journals Zinc, Zinc Transporters, and Cadmium Cytotoxicity in a Cell Culture Model of Human Urothelium

Toxics ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 94
Author(s):  
Soisungwan Satarug ◽  
Scott H. Garrett ◽  
Seema Somji ◽  
Mary Ann Sens ◽  
Donald A. Sens
Keyword(s):  
Dna Methylation ◽  
Cell Culture ◽  
Cell Viability ◽  
Zinc Transporters ◽  
Cell Culture Model ◽  
Induced Expression ◽  
Glutathione Biosynthesis ◽  
Culture Model ◽  
A Cell ◽  
Cd Exposure

We explored the potential role of zinc (Zn) and zinc transporters in protection against cytotoxicity of cadmium (Cd) in a cell culture model of human urothelium, named UROtsa. We used real-time qRT-PCR to quantify transcript levels of 19 Zn transporters of the Zrt-/Irt-like protein (ZIP) and ZnT gene families that were expressed in UROtsa cells and were altered by Cd exposure. Cd as low as 0.1 µM induced expression of ZnT1, known to mediate efflux of Zn and Cd. Loss of cell viability by 57% was seen 24 h after exposure to 2.5 µM Cd. Exposure to 2.5 µM Cd together with 10–50 µM Zn prevented loss of cell viability by 66%. Pretreatment of the UROtsa cells with an inhibitor of glutathione biosynthesis (buthionine sulfoximine) diminished ZnT1 induction by Cd with a resultant increase in sensitivity to Cd cytotoxicity. Conversely, pretreatment of UROtsa cells with an inhibitor of DNA methylation, 5-aza-2’-deoxycytidine (aza-dC) did not change the extent of ZnT1 induction by Cd. The induced expression of ZnT1 that remained impervious in cells treated with aza-dC coincided with resistance to Cd cytotoxicity. Therefore, expression of ZnT1 efflux transporter and Cd toxicity in UROtsa cells could be modulated, in part, by DNA methylation and glutathione biosynthesis. Induced expression of ZnT1 may be a viable mechanistic approach to mitigating cytotoxicity of Cd.

scholarly journals A cell-based drug delivery platform for treating central nervous system inflammation

2021 ◽  
Author(s):  
Oren Levy ◽  
Veit Rothhammer ◽  
Ivan Mascanfroni ◽  
Zhixiang Tong ◽  
Rui Kuai ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Central Nervous System ◽  
Nervous System ◽  
Central Nervous System Inflammation ◽  
Delivery Platform ◽  
A Cell

Development of a cell delivery system using alginate microbeads for tissue regeneration

2016 ◽  
Vol 4 (20) ◽  
pp. 3515-3525 ◽  
Author(s):  
Shirae K. Leslie ◽  
Anthony M. Nicolini ◽  
Gobalakrishnan Sundaresan ◽  
Jamal Zweit ◽  
Barbara D. Boyan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Delivery System ◽  
Tissue Regeneration ◽  
Cell Delivery ◽  
Adipose Derived Stem Cells ◽  
Viable Cells ◽  
A Cell

Alginate microbeads incorporating adipose-derived stem cells (ASCs) have potential for delivering viable cells capable of facilitating tissue regeneration.

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