scholarly journals Nanofibrillar cellulose wound dressing supports the growth and characteristics of human mesenchymal stem/stromal cells without cell adhesion coatings

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Jasmi Kiiskinen ◽  
Arto Merivaara ◽  
Tiina Hakkarainen ◽  
Minna Kääriäinen ◽  
Susanna Miettinen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Adhesion ◽  
Cell Viability ◽  
Cell Survival ◽  
Cell Transplantation ◽  
Stromal Cells ◽  
Wound Dressing ◽  
Nanofibrillar Cellulose ◽  
Cell Scaffold ◽  
A Cell

Abstract Background In the field of regenerative medicine, delivery of human adipose-derived mesenchymal stem/stromal cells (hASCs) has shown great promise to promote wound healing. However, a hostile environment of the injured tissue has shown considerably to limit the survival rate of the transplanted cells, and thus, to improve the cell survival and retention towards successful cell transplantation, an optimal cell scaffold is required. The objective of this study was to evaluate the potential use of wood-derived nanofibrillar cellulose (NFC) wound dressing as a cell scaffold material for hASCs in order to develop a cell transplantation method free from animal-derived components for wound treatment. Methods Patient-derived hASCs were cultured on NFC wound dressing without cell adhesion coatings. Cell characteristics, including cell viability, morphology, cytoskeletal structure, proliferation potency, and mesenchymal cell and differentiation marker expression, were analyzed using cell viability assays, electron microscopy, immunocytochemistry, and quantitative or reverse transcriptase PCR. Student’s t test and one-way ANOVA followed by a Tukey honestly significant difference post hoc test were used to determine statistical significance. Results hASCs were able to adhere to NFC dressing and maintained high cell survival without cell adhesion coatings with a cell density-dependent manner for the studied period of 2 weeks. In addition, NFC dressing did not induce any remarkable cytotoxicity towards hASCs or alter the morphology, proliferation potency, filamentous actin structure, the expression of mesenchymal vimentin and extracellular matrix (ECM) proteins collagen I and fibronectin, or the undifferentiated state of hASCs. Conclusions As a result, NFC wound dressing offers a functional cell culture platform for hASCs to be used further for in vivo wound healing studies in the future.

A cell viability assessment approach based on electrical wound-healing impedance characteristics

2019 ◽  
Vol 124-125 ◽  
pp. 25-32 ◽  
Author(s):  
Mingji Wei ◽  
Yecheng Zhang ◽  
Guoxiao Li ◽  
Ying Ni ◽  
Siqi Wang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Viability ◽  
Viability Assessment ◽  
Impedance Characteristics ◽  
Assessment Approach ◽  
A Cell

scholarly journals Preparation of Nanofibers with Renewable Polymers and Their Application in Wound Dressing

2016 ◽  
Vol 2016 ◽  
pp. 1-17 ◽  
Author(s):  
Ying Zhao ◽  
Yihui Qiu ◽  
Huanhuan Wang ◽  
Yu Chen ◽  
Shaohua Jin ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Cell Adhesion ◽  
Specific Surface ◽  
Wound Dressing ◽  
Recent Progress ◽  
High Specific Surface Area ◽  
High Porosity ◽  
Renewable Polymers ◽  
Good Biocompatibility

Renewable polymers have attracted considerable attentions in the last two decades, predominantly due to their environmentally friendly properties, renewability, good biocompatibility, biodegradability, bioactivity, and modifiability. The nanofibers prepared from the renewable polymers can combine the excellent properties of the renewable polymer and nanofiber, such as high specific surface area, high porosity, excellent performances in cell adhesion, migration, proliferation, differentiation, and the analogous physical properties of extracellular matrix. They have been widely used in the fields of wound dressing to promote the wound healing, hemostasis, skin regeneration, and treatment of diabetic ulcers. In the present review, the different methods to prepare the nanofibers from the renewable polymers were introduced. Then the recent progress on preparation and properties of the nanofibers from different renewable polymers or their composites were reviewed; the application of them in the fields of wound dressing was emphasized.

scholarly journals Hyaluronate can function as a cell adhesion molecule and CD44 participates in hyaluronate recognition.

1990 ◽  
Vol 172 (1) ◽  
pp. 69-75 ◽  
Author(s):  
K Miyake ◽  
C B Underhill ◽  
J Lesley ◽  
P W Kincade
Keyword(s):  
Cell Adhesion ◽  
Stromal Cells ◽  
Divalent Cations ◽  
Cell Types ◽  
Lymphoid Cells ◽  
Hybridoma Cells ◽  
Antibody Treatment ◽  
A Cell ◽  
Adhesive Interactions ◽  
Potential Ligand

A cell adhesion model was previously used to select a series of monoclonal antibodies (mAbs), which were subsequently found to recognize CD44/Pgp-1. Interest in these reagents increased with the finding that they totally inhibited production of lymphoid or myeloid cells in long-term bone marrow cultures. Further investigation has now revealed that hyaluronate is a potential ligand for CD44 and that hyaluronate recognition accounts for the adhesion between B lineage hybridoma and stromal cells. The hybridoma cells adhered to hyaluronate-coated plastic wells as well as to monolayers of stromal cells. The adhesion in both cases was inhibited by treatment with hyaluronidases, and did not require divalent cations. Addition of exogenous hyaluronate also diminished binding of lymphoid cells to stromal cells. One of several mAbs to Pgp-1/CD44 was particularly effective at blocking these interactions. Since hyaluronate and Pgp-1/CD44 were present on both cell types, experiments were done to determine the cellular location of interacting molecules required for the adhesion process. Treatment of lymphoid cells with an anti-Pgp-1/CD44 antibody was more inhibitory than antibody treatment of the stromal cells. Conversely, hyaluronidase treatment of stromal cells reduced subsequent binding more than treatment of the lymphoid cells. Adhesive interactions that involve hyaluronate and CD44 could contribute to a number of cell recognition processes, including ones required for normal lympho-hemopoiesis.

Peptide-modified methacrylated glycol chitosan hydrogels as a cell-viability supporting pro-angiogenic cell delivery platform for human adipose-derived stem/stromal cells

2018 ◽  
Vol 107 (3) ◽  
pp. 571-585 ◽  
Author(s):  
Jobanpreet Dhillon ◽  
Stuart A. Young ◽  
Stephen E. Sherman ◽  
Gillian I. Bell ◽  
Brian G. Amsden ◽  
...  
Keyword(s):  
Cell Viability ◽  
Stromal Cells ◽  
Cell Delivery ◽  
Glycol Chitosan ◽  
Delivery Platform ◽  
A Cell ◽  
Chitosan Hydrogels

93. Mechanisms Implicated In Cell Proliferation and Cell Survival Induced By Recombinant Losac, A Cell Adhesion Molecule From Lonomia oblique

Toxicon ◽  
2012 ◽  
Vol 60 (2) ◽  
pp. 142-143
Author(s):  
Miryam P. Alvarez-Flores ◽  
Cesar M. Remuzgo ◽  
Yara Cury ◽  
Rosemary V. Bosch ◽  
Beatriz B. Vaz-De-Lima ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Adhesion ◽  
Cell Survival ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
A Cell

Microenvironment Shields Myeloma From Adoptive Immunotherapy by Cell Adhesion Mediated Immune Resistance (CAM-IR),

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 4039-4039
Author(s):  
Sanne J de Haart ◽  
Monique C. Minnema ◽  
Tineke Aarts-Riemens ◽  
Niels Bovenschen ◽  
Henk M. Lokhorst ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
T Cell ◽  
Stromal Cells ◽  
Critical Role ◽  
Immune Resistance ◽  
Accessory Cells ◽  
Number Of Patients ◽  
Apoptosis Protein ◽  
A Cell ◽  
First Time

Abstract Abstract 4039 Cellular immunotherapy can induce long term remissions in multiple myeloma (MM) patients. Despite reported successes in a limited number of patients, the immune system fails to completely eradicate MM in the majority, proving the ability of MM cells to evade cellular immunity. The nature and mechanism of this immune escape remains not fully understood. Most studies have focused on mechanisms by which tumor cells down-regulate their antigenicity or suppress immune effector cells. Here we show a critical role for the tumor microenvironment in inducing a cell-adhesion mediated ability in MM cells to resist T cell mediated killing; a mechanism similar to cell-adhesion mediated drug resistance (CAM-DR). We used a compartment-specific bioluminescence-based viability assay, in which luciferase transduced MM cell lines were co-cultured with MM-reactive CD4+ and CD8+cytotoxic T cells (CTLs) in presence and absence of accessory cells. In these co-culture assays, we demonstrated the effective CTL-mediated lysis of MM cells. However, this lysis was significantly decreased in the presence of accessory cells, such as bone marrow stromal cells, including patient derived stromal cells, activated endothelial cells and fibroblasts. We identified that the reduction of CTL-mediated lysis was predominantly due to the induction of a cell-cell contact mediated resistance in MM cells against CTL killing, proving for the first time the existence of a cell-adhesion mediated immune resistance (CAM-IR). In support to this, blocking the adhesion of MM cells to accessory cells with an ICAM-1 antibody or with RGD peptide, which is a ligand for cell surface integrins, abrogated the resistance of MM cells against CTL-mediated lysis. Intracellular mechanisms of this immune resistance were evaluated by western blot. Adherence of MM cells to accessory cells significantly increase the expression levels of survivin in MM cells, a well known inhibitor of apoptosis protein (IAP), which blocks the CTL mediated apoptotic events via inhibiting the phosphorylation of caspase-3. Our current focus is the restoration of MM cell sensitivity to T cell mediated killing via downregulation of survivin expression. In conclusion, we report for the first time the potentially important role of microenvironment in compromising immunotherapy by the induction of CAM-IR. Our results suggest that immunotherapy may be improved by modulating the interaction of MM with its microenvironment Disclosures: No relevant conflicts of interest to declare.

Response of Normal Canine Prostate Tissue to Thermal Therapy

2008 ◽  
Author(s):  
Sepideh Khoshnevis ◽  
Kenneth R. Diller
Keyword(s):  
Flow Cytometry ◽  
Thermal Stress ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Expression ◽  
Cell Viability ◽  
Cell Survival ◽  
Stromal Cells ◽  
Thermal Therapy ◽  
Heat Shock Protein Expression

The goal of this study was to evaluate post-thermal stress cell survival in canine prostatic epithelial and stromal cells. Epithelial and stromal cells isolated from canine prostate were exposed to thermal stress using a water bath. Post-thermal stress cell viability was measured by flow cytometry and analyzed using FlowJo software. An ongoing analysis also measures Heat Shock Protein expression for the same stress protocols.

scholarly journals Oxygen producing microscale spheres affect cell survival in conditions of oxygen-glucose deprivation in a cell specific manner: implications for cell transplantation

2018 ◽  
Vol 6 (10) ◽  
pp. 2571-2577 ◽  
Author(s):  
Heike Newland ◽  
Dimitri Eigel ◽  
Anne E. Rosser ◽  
Carsten Werner ◽  
Ben Newland
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Survival ◽  
Cell Transplantation ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Specific Manner ◽  
A Cell

Oxygen-glucose deprivation detrimentally affected mesenchymal stem cells, which could be reversed by the addition of oxygen producing spheres.

scholarly journals Biological Performance of Electrospun Polymer Fibres

Materials ◽  
2019 ◽  
Vol 12 (3) ◽  
pp. 363 ◽  
Author(s):  
Ivan Hall Barrientos ◽  
Graeme MacKenzie ◽  
Clive Wilson ◽  
Dimitrios Lamprou ◽  
Paul Coats
Keyword(s):  
Cell Proliferation ◽  
Cell Adhesion ◽  
Cell Viability ◽  
Acid Group ◽  
Adhesion Assay ◽  
Biological Responses ◽  
Polymeric Scaffolds ◽  
Biological Studies ◽  
A Cell ◽  
Biological Performance

The evaluation of biological responses to polymeric scaffolds are important, given that the ideal scaffold should be biocompatible, biodegradable, promote cell adhesion and aid cell proliferation. The primary goal of this research was to measure the biological responses of cells against various polymeric and collagen electrospun scaffolds (polycaprolactone (PCL) and polylactic acid (PLA) polymers: PCL–drug, PCL–collagen–drug, PLA–drug and PLA–collagen–drug); cell proliferation was measured with a cell adhesion assay and cell viability using 5-bromo-2′-deoxyuridine (BrdU) and resazurin assays. The results demonstrated that there is a distinct lack of growth of cells against any irgasan (IRG) loaded scaffolds and far greater adhesion of cells against levofloxacin (LEVO) loaded scaffolds. Fourteen-day studies revealed a significant increase in cell growth after a 7-day period. The addition of collagen in the formulations did not promote greater cell adhesion. Cell viability studies revealed the levels of IRG used in scaffolds were toxic to cells, with the concentration used 475 times higher than the EC50 value for IRG. It was concluded that the negatively charged carboxylic acid group found in LEVO is attracting positively charged fibronectin, which in turn is attracting the cell to adhere to the adsorbed proteins on the surface of the scaffold. Overall, the biological studies examined in this paper are valuable as preliminary data for potential further studies into more complex aspects of cell behaviour with polymeric scaffolds.

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