Adhatoda vasica and Calotropis procera as a resource of novel chemical compounds, their biological bioluminescence assay, and investigation of morphological features of bacterial growth through advanced technologies

Author(s):  
Waseem Ahmed ◽  
Rafia Azmat ◽  
Nabila Chendouh‐Brahmi ◽  
Ijaz Hussain ◽  
Rasheed Ahmed ◽  
...  
Author(s):  
Sabreen A Kamal ◽  
Ishraq A Salih ◽  
Hawraa Jawad Kadhim ◽  
Zainab A Tolaifeh

Red rose or roselle (beauty rose ) is natively known as red tea belong to Malvaceae, it is flowers use traditionally for antihypertensive hepato protective, anticancer,antidiabetic,antibacterial, cytotoxicity and antidiarreal, By preparing red tea from it's flower. In this study, we extract chemical compounds by using two solvent which are Ethanol, Ethyl acetate. so we can extract Anthocyanin which is responsible for red colour of flower with many chemical compounds. then study the effect of these extracts on 5 genera from Enterobacteriacaea which can cause diarrheae (Shigella, Salmonella, Escherichia coli, Proteus and Klebsiella ) by preparing 3 concentrations for each solvent (250, 500, 750 ) mg/ml, and control then compare with two antibiotic (Azereonam 30 mg/ml and Bacitracin 10 mg/ml ) these extracts revealed obvious inhibition zone in bacterial growth.


2007 ◽  
Vol 39 (5) ◽  
pp. 401-407 ◽  
Author(s):  
Begoña AGUIRRE-HUDSON ◽  
Tetsuo KOKUBUN ◽  
Brian M. SPOONER ◽  
Leif TIBELL

Abstract:The morphological features and chemical compounds found in the first European collection of Calicium victorianum are compared with type material from Australia of C. piperatum F. Wilson. The phylogenetic relationships of the species are discussed by comparing its nuclear rDNA ITS1-5.8S-ITS2 with that of other species of Calicium.


2018 ◽  
Vol 10 (12) ◽  
pp. 262
Author(s):  
Danúbio Lopes da Silva ◽  
Marcos Aurélio Figueiredo dos Santos ◽  
Jose Weverton Almeida Bezerra ◽  
Cícero dos Santos Leandro ◽  
Maria Daniele Pereira Rodrigues ◽  
...  

Through secondary metabolism, plants produce chemical compounds which when released into the environment can have effects on other species, such effect is called allelopathy. In this research we evaluated the Cordia oncocalyx (Boraginaceae) allelopathic potential against two weed species, Cenchrus echinatus and Calotropis procera, as well as quantified its phenolic compounds using HPLC-DAD. The experimental design was completely randomized with six treatments and one control group (distilled water). Each treatment consisted of four replicates with 25 seeds each, totaling 100 seeds per treatment. The variables analyzed were: germinating potential, germination velocity and seedling development. The hot and cold infusion extracts were prepared with 50 g of each of the three parts of the donor plant (bark, leaves and roots) immersed in 500 ml of distilled water at 100 °C (hot infusion) and 25 °C (cold infusion). The experiments were mounted in Petri dishes having substrate filter paper embedded in 3 mL of the extracts (treatments) and conducted in a germination chamber. Hot and cold infusion extracts of the C. oncocalyx leaves, barks and roots presented allelopathic activity on C. echinatus and C. procera germination and development, with the hot bark extract promoting such effect with a greater intensity. The allelopathic effect observed may be due to the presence of allelochemicals such as caffeic acid, quercentin and kaempferol present in C. oncocalyx leaves, barks and roots.


2019 ◽  
Vol 4 (2) ◽  
pp. 12-16
Author(s):  
Fera Sartika

Coconut products are known to have anti-bacterial activity. One product of coconut is coconut water. Coconut water has benefits in food processing, especially for health. Coconut water contains tannin or antidote (anti-poison) which is antibacterial which will inhibit bacterial growth. This study aims to determine the antimicrobial effects of coconut water by testing the inhibition of Salmonella typhi and Escherichia coli bacteria. This type of research uses experimental or experimental methods. Before the inhibitory test is carried out, phytochemical tests of coconut water are carried out such as flavonoids, alkaloids, tannins, saponins, and steroids. The results showed that coconut water does not contain the chemical compounds of flavonoids, alkaloids, tannins, saponins, and steroids. The results of the inhibitory test indicate that coconut water cannot inhibit the growth of Salmonella typhi and Escherichia coli bacteria.


2021 ◽  
Vol 11 ◽  
pp. 33-39
Author(s):  
A.F. Ogundola ◽  
T.A. Yekeen ◽  
R.A. Arotayo ◽  
A.O. Akintola ◽  
A.O. Ibrahim ◽  
...  

Calotropis procera has been widely explored in ethnomedicine to cure several ailments such as leprosy, fever, elephantiasis, menorrhagia, and snakebite. It is also used as a purgative, anthelmintic, anticoagulant, anticancer, antipyretic, analgesic, and carminative. In addition to its traditional use as coagulants, the leaves and seeds of Calotropis procera could be used in food fortifications to combat nutrient deficiencies as reflected in its bioactive components. The increase in its use might be associated with the level of many bioactive components, which provide nutritional and health benefits. Thus, the leaves and seeds were analyzed for their bioactive components and characterized for nutrient values using the procedures of the Association of Official Analytical Chemists. The chemical analyses results showed that the leaf and the seed contained (g/100 g dry weight) moisture (8.11g, 9.53g), crude protein (26.69g, 14.48g), crude fiber (7.54, 15.73), crude fat (21.70, 6.29), ash (5.32, 3.69) and carbohydrate (30.64, 50.29), respectively. The leaves and seeds contained zinc (1.20, 0.60 mg/100 g), potassium (33.60, 30.30 mg/100 g) and iron (36.90, 12.90 mg/100g), respectively. The fatty acids profile revealed that the leaves and the seed oils contained a low level of saturated palmitic acid (3.01, 7.70 g/100g) and a high level of monounsaturated oleic acid (10.31, 27.90 g/100g) and polyunsaturated acids (11.63, 18.53 g/100g), respectively. It is established that the chemical compounds in the Calotropis procera seeds and leaves could be beneficial for therapeutic and dietary purposes. Thus, it can be accepted that the Calotropis procera plant may be used as medicine and food fortificants.


Author(s):  
R. Courtoy ◽  
L.J. Simar ◽  
J. Christophe

Several chemical compounds induce amine liberation from mast cells but do not necessarily provoque the granule expulsion. For example, poly-dl-lysine induces modifications of the cellular membrane permeability which promotes ion exchange at the level of mast cell granules. Few of them are expulsed but the majority remains in the cytoplasm and appears less dense to the electrons. A cytochemical analysis has been performed to determine the composition of these granules after the polylysine action.We have previously reported that it was possible to demonstrate polyanions on epon thin sections using a cetylpyridinium ferric thiocyanate method. Organic bases are selectively stained with cobalt thiocyanate and the sulfhydryle groups are characterized with a silver methenamine reaction. These techniques permit to reveal the mast cell granule constituents, i.e. heparin, biogenic amines and basic proteins.


Author(s):  
Necip Güven ◽  
Rodney W. Pease

Morphological features of montmorillonite aggregates in a large number of samples suggest that they may be formed by a dendritic crystal growth mechanism (i.e., tree-like growth by branching of a growth front).


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