Urinary Measurement of Epigenetic DNA Modifications: A Non-Invasive Assessment of the Whole-Body Epigenetic Status in Healthy Subjects and Colorectal Cancer Patients

The studyTaylor S, Mallett S, Beare S et al. Diagnostic accuracy of whole-body MRI versus standard imaging pathways for metastatic disease in newly diagnosed colorectal cancer: the prospective Streamline C trial. Lancet Gastroenterol Hepatol 2019;4:529-37.This project was funded by the NIHR Health Technology Assessment Programme (project number 10/68/01).To read the full NIHR Signal, go to https://discover.dc.nihr.ac.uk/content/signal-000797/identifying-metastatic-disease-in-colorectal-cancer-with-whole-body-mri

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Platelet indices in patients with colorectal cancer

Open Medicine ◽

10.2478/s11536-009-0077-7 ◽

2010 ◽

Vol 5 (3) ◽

pp. 365-368 ◽

Cited By ~ 3

Author(s):

Bülent Karagöz ◽

İlker Sücüllü ◽

Özkan Sayan ◽

Oğuz Bilgi ◽

Tolga Tuncel ◽

...

Keyword(s):

Colorectal Cancer ◽

Platelet Count ◽

Cancer Patients ◽

Healthy Subjects ◽

Study Data ◽

Distribution Width ◽

Platelet Indices ◽

Long Time ◽

Colorectal Cancer Patients ◽

Record Review

AbstractThe interaction between cancer cells and platelets has been known for a long time. Although platelet indices have been also investigated in several clinical settings, it has not been exactly demonstrated in cancer patients. We investigated platelet indices in colorectal cancer patients and compared with healthy subjects. Two hundred and twenty-one colorectal cancer patients and 110 healthy subjects were enrolled into the retrospective study. Data were obtained from computerized medical records of our hospital. Medical record review was performed for all patients regarding thrombocyte indices. Platelet count (325.000/mm3 ± 265.000/mm3 vs 267.000/mm3 ± 67.000/mm3; p=0.025; respectively) and plateletcrit (Pct) (0.25% ± 0.10 vs 0.21 ± 0.05; p<0.001; respectively) were increased in patients compared with healthy subjects while mean platelet volume (MPV) and platelet distribution width (PDW) were similar. The platelet indices were not related to existence of metastasis or acute abdomen. Platelet count and Pct, but not MPV and PDW, are elevated in colorectal cancer patients. Future studies that investigate platelet morphology, function, and putative role of platelets in tumorigenesis and metatasis should be established.

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HUMAN DNA QUANTIFICATION IN THE STOOLS OF PATIENTS WITH COLORECTAL CANCER

Arquivos de Gastroenterologia ◽

10.1590/s0004-28032015000400008 ◽

2015 ◽

Vol 52 (4) ◽

pp. 293-298 ◽

Cited By ~ 5

Author(s):

Yolanda TEIXEIRA ◽

Jacqueline Miranda LIMA ◽

Maria Luiza Almeida Prado Oliveira SOUZA ◽

Pedro AGUIAR Jr ◽

Tiago Donizetti SILVA ◽

...

Keyword(s):

Colorectal Cancer ◽

Cancer Cells ◽

Cancer Patients ◽

Roc Curve ◽

Dna Quantification ◽

Non Invasive ◽

Human Dna ◽

The World ◽

Invasive Method ◽

Colorectal Cancer Patients

Background - Colorectal cancer is one of the main cause of cancer in the world. Colonoscopy is the best screen method, however the compliance is less than 50%. Quantification of human DNA (hDNA) in the feces may be a possible screen non-invasive method that is a consequence of the high proliferation and exfoliation of cancer cells. Objective - To quantify the human DNA in the stools of patients with colorectal cancer or polyps. Methods - Fifty patients with CRC, 26 polyps and 53 with normal colonoscopy were included. Total and human DNA were analyzed from the frozen stools. Results - An increased concentration of hDNA in the stools was observed in colorectal cancer patients compared to controls and polyps. Tumors localized in the left side of the colon had higher concentrations of hDNA. There were no difference between polyps and controls. A cut off of 0.87 ng/mL of human DNA was determined for colorectal cancer patients by the ROC curve, with a sensitivity of 66% and a specificity of 86.8%. For polyps the cut off was 0.41, the sensitivity was 41% and the specificity 77.4%. Conclusion - A higher concentration of hDNA had been found in colorectal cancer patients The quantification of hDNA from the stools can be a trial method for the diagnosis of colorectal cancer.

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Plasma protein-bound sialic acid in patients with colorectal polyps of known histology

Clinical Chemistry ◽

10.1093/clinchem/37.2.200 ◽

1991 ◽

Vol 37 (2) ◽

pp. 200-204 ◽

Cited By ~ 1

Author(s):

Shahram Shahangian ◽

Herbert A Fritsche ◽

John I Hughes ◽

Richard S Foemmel ◽

Nonda Katopodis

Keyword(s):

Colorectal Cancer ◽

Sialic Acid ◽

Cancer Patients ◽

Healthy Subjects ◽

Mean Value ◽

Colorectal Polyps ◽

Hyperplastic Polyps ◽

The Mean ◽

Colorectal Cancer Patients ◽

Colorectal Adenocarcinomas

Abstract Protein-bound sialic acid (PBSA) was measured in serial plasma specimens from 62 healthy subjects, 48 patients with colorectal polyps, and 30 patients with colorectal adenocarcinomas. The mean plasma PBSA concentration in healthy smokers was significantly greater than that in healthy nonsmokers and healthy ex-smokers (P less than 0.0001). Villoglandular polyps were associated with higher plasma PBSA values than were the most benign hyperplastic polyps (P less than 0.025). Patients with the most neoplastic villoglandular and villous polyps had significantly greater (P less than 0.010-0.050) plasma PBSA values than healthy subjects. Polypectomy decreased the mean PBSA value significantly to the mean value for healthy subjects only for patients with villoglandular (P less than 0.010) or villous (P less than 0.050) polyps. Colorectal cancer patients had mean plasma PBSA concentrations significantly greater than those for the healthy subjects (P much less than 0.001) and the polyp patients (P much less than 0.001). Surgery significantly reduced (P less than 0.025) the mean PBSA value for the cancer patients to the mean PBSA value observed for the healthy subjects.

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Precision of Lunar Dual-Energy X-ray Absorptiometry (iDXA) in measuring body composition among colorectal cancer patients and healthy subjects

Clinical Nutrition ESPEN ◽

10.1016/j.clnesp.2021.05.025 ◽

2021 ◽

Author(s):

Hege Berg Henriksen ◽

Dena Helene Alavi ◽

Rune Blomhoff

Keyword(s):

Colorectal Cancer ◽

Body Composition ◽

Cancer Patients ◽

Healthy Subjects ◽

Dual Energy ◽

X Ray ◽

Colorectal Cancer Patients

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piRNA-823 Is a Unique Potential Diagnostic Non-Invasive Biomarker in Colorectal Cancer Patients

Genes ◽

10.3390/genes12040598 ◽

2021 ◽

Vol 12 (4) ◽

pp. 598

Author(s):

Norhan A. Sabbah ◽

Wael M. Abdalla ◽

Walid A. Mawla ◽

Nagla AbdAlMonem ◽

Amal F. Gharib ◽

...

Keyword(s):

Colorectal Cancer ◽

Cancer Patients ◽

Characteristic Curve ◽

Area Under The Curve ◽

Serum Levels ◽

P Element ◽

Serum Samples ◽

Expression Levels ◽

Non Invasive ◽

Colorectal Cancer Patients

Early detection of colorectal cancer (CRC) is the most important factor in deciding its prognosis, so the need to develop an accurate screening test is a must. P-element induced wimpy testis (PIWI) RNA-823 (piR-823) is one of the first piRNAs recognized to be linked to malignancy. We aimed to investigate the expression levels of piR-823 in both serum and tissues of colorectal cancer patients and the ability to use its serum level as a non-invasive diagnostic biomarker to detect colorectal cancer. We determined piR-823 expression levels in 84 serum samples of CRC patients, 75 serum samples of healthy controls, and biological specimens obtained from the 84 patients with colorectal cancer from both the tumor tissues and the normal neighboring tissues using quantitative real-time reverse transcriptase-PCR. We showed that piR-823 had significantly higher serum and tissue expression levels in CRC patients compared to the controls. We observed a significant positive correlation between piR-823 serum levels and the staging of CRC, with significantly higher levels exhibiting advanced stages of CRC (III and IV). This translates into poorer differentiation and lymph node metastasis. The receiver operating characteristic curve (ROC curve) test showed 83.3% sensitivity and 89.3% specificity at a cut-off value of >5.98-fold change, with an area under the curve of 0.933 (p < 0.0001) concerning the ability of piR-823 in diagnosing patients with colorectal carcinoma. piR-823 expression is upregulated in colorectal cancer patients’ serum and tissues, and it can be used as a diagnostic noninvasive biomarker for CRC.

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Whole-body MRI is effective for identifying metastatic disease in colorectal cancer patients

10.3310/signal-000797 ◽

2019 ◽

Author(s):

Keyword(s):

Colorectal Cancer ◽

Cancer Patients ◽

Metastatic Disease ◽

Whole Body ◽

Whole Body Mri ◽

Colorectal Cancer Patients

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Genotyping of the Lactase-Phlorizin Hydrolase C/T-13910 Polymorphism by Means of a New Rapid Denaturing High-Performance Liquid Chromatography-Based Assay in Healthy Subjects and Colorectal Cancer Patients

CrossRef Listing of Deleted DOIs ◽

10.1177/1087057107301328 ◽

2007 ◽

Vol 12 (5) ◽

pp. 733-739 ◽

Cited By ~ 8

Author(s):

Ada Piepoli ◽

Enrico Schirru ◽

Angela Mastrorilli ◽

Annamaria Gentile ◽

Rosa Cotugno ◽

...

Keyword(s):

Colorectal Cancer ◽

Colon Cancer ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Cancer Patients ◽

Healthy Subjects ◽

High Performance ◽

Genetic Variant ◽

Increased Risk ◽

Colorectal Cancer Patients

Adult-type hypolactasia results from the progressive decline of lactase-phlorizin hydrolase activity in enterocytes after weaning. Lactase nonpersistence may determine a primary lactose intolerance with reduced diary product consumption, which is possibly related to an increased risk of colon cancer. Recently, a genetic variant C/T—13910 upstream of the lactase-phlorizin hydrolase ( LCT) gene has been strongly correlated with the lactase persistence/nonpersistence trait in both family and case-control studies. The authors validate a denaturing high-performance liquid chromatography (dHPLC)—based assay versus conventional genotype sequencing in detecting the C/T—13910 polymorphism of LCT and evaluate its prevalence in 2 different Italian geographical areas and in colorectal cancer patients. DNA samples of 157 healthy subjects and 124 colon cancer patients from Apulia and of 97 healthy subjects from Sardinia were evaluated for the C/T—13910 polymorphism by dHPLC, sequencing, and restriction fragment length polymorphism (RFLP). Under optimized conditions, dHPLC was as sensitive as DNA sequencing and detected a new genetic variant (T/C-13913) in 2 individuals that was not identified by RFLP assay. Frequency of lactase nonpersistence genotype (C/C—13910) was similar in healthy subjects from 2 different Italian geographical areas and not increased in patients with colorectal cancer. The results indicate that the dHPLC method may be used as a rapid, noninvasive, and laborsaving screening tool for genotyping C/T—13910 polymorphism, with high success, low cost, and reproducibility. ( Journal of Biomolecular Screening 2007:733-739)

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