Comparative proteomic analysis of peripheral blood eosinophils from healthy donors and atopic dermatitis patients with eosinophilia

Investigations of the mRNA expression of apoptosis intracellular regulators, bcl-2 and bcl-xL antagonists and bax, bcl-xL agonists of cellular destruction as well as mRNA expression of IL-5 were carried out. As a result of investigation of potential role of IL-5 in the regulation of programmable bcl-2-dependent destruction we found the increase of vitality and mRNA expression stimulation of bcl-2 peripheral blood eosinophils in patients with bronchial asthma (BA). It was found that fresh-isolated peripheral blood eosinophils in all investigated groups expressed bax and bcl-xL mRNA, bcl-xS had the less activity. In peripheric blood eosinophils of healthy donors the bcl-2 expression was not found, however, the increase of mRNA expression by IL-5 was shown in group of patients with bronchial asthma and, possibly connected with this, the appearance of bcl-2 activity. Thus, the decrease of apoptotic activity in peripheral blood eosinophils in patients with bronchial asthma may lead to the increase of eosinophil portion that is subjected to necrotic destruction and this may significantly contribute into bronchial asthma pathogenesis.

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LOCAL AND SYSTEMIC IMMUNE PARAMETERS IN SEVERE ATOPIC DERMATITIS AND CUTANEOUS T-CELL LYMPHOMA PATIENTS

Rossiiskii Allergologicheskii ZHurnal ◽

10.36691/rja601 ◽

2013 ◽

Vol 10 (5) ◽

pp. 13-21

Author(s):

D D Niyazov ◽

E S Fedenko ◽

M N Boldyreva ◽

D Yu Trofimov

Keyword(s):

Gene Expression ◽

Atopic Dermatitis ◽

T Cell ◽

Peripheral Blood ◽

Cell Lymphoma ◽

Severe Atopic Dermatitis ◽

Cutaneous T Cell Lymphoma ◽

Healthy Donors ◽

Genes Expression ◽

Skin Samples

Background.. To investigate expression of cytokines genes parameters in skin and blood in severe atopic dermatitis (AD) and cutaneous T-cell lymphoma (CTCL) patients comparing with healthy donors. Materials and methods. 20 severe AD patients, 20 CTCL patients and 20 healthy donors were included in the study. Skin samples and peripheral blood were used as material for immunological study. Interleukins — (IL)1B, IL2, IL2r, IL4, IL5, IL6, IL7, IL8, IL10, IL12A, IL12B, IL15 (total), IL15 , IL17A, IL18, IL23, IL28, IL29, Interferon γ (IFNγ), tumor necrosis factor α (TNFα), transforming growth factor beta 1 (TGFB1), forkhead box P3 (FOXP3) gene expression was defined in the skin and peripheral blood of severe AD patients, CTCL patients and healthy donors by real-time reverse transcription polymerase chain reaction (RT-PCR). Results. Statistically significant increase of cytokines genes IL4,IL12B,IL17A, TNFα in peripheral blood of severe AD patients compared with CTCL patients was marked. Studying of skin samples from CTCL patients has shown statistically significant increase of cytokines IL8, IL10, IL15, IFNγ genes expression and decrease of IL18 gene expression in comparison with skin samples from severe AD patients. Conclusion. Obtained cytokines genes expression cytokines genes parameters in peripheral blood of severe AD and CTCL patients had a certain similarities consisting of increased IL8, IFNγ and decreased IL6, IL23 genes expression in comparison with healthy donors. Substantial differences in peripheral blood of patients in comparison with healthy donors: increased IL-5, IL-12B genes expression in AD patients and decreased IL-8, IL-12A genes expression in CTCL patients, at the same time increased expression of IL-6, IL-8, IL-10, IFNγ genes in severe AD and CTCL patients were shown.

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Comparative proteomic analysis of endothelial cells progenitor cells derived from cord blood- and peripheral blood for cell therapy

Biomaterials ◽

10.1016/j.biomaterials.2012.11.017 ◽

2013 ◽

Vol 34 (6) ◽

pp. 1669-1685 ◽

Cited By ~ 13

Author(s):

Jumi Kim ◽

Young-Joo Jeon ◽

Hye Eun Kim ◽

Jeong Min Shin ◽

Hyung Min Chung ◽

...

Keyword(s):

Endothelial Cells ◽

Cell Therapy ◽

Cord Blood ◽

Progenitor Cells ◽

Peripheral Blood ◽

Proteomic Analysis ◽

Comparative Proteomic Analysis

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Local and systemic immune response in patients withsevere atopic dermatitis

Rossiiskii Allergologicheskii ZHurnal ◽

10.36691/rja771 ◽

2011 ◽

Vol 8 (5) ◽

pp. 10-15

Author(s):

T M Filimonova ◽

Ol'ga Gur'evna Elisyutina ◽

E S Fedenko ◽

D D Niyazov ◽

M N Boldyreva ◽

...

Keyword(s):

Gene Expression ◽

Immune Response ◽

Atopic Dermatitis ◽

Peripheral Blood ◽

Transforming Growth Factor ◽

Cytokine Gene Expression ◽

Cytokine Gene ◽

Healthy Individuals ◽

Th2 Response ◽

Healthy Donors

Background. to comparatively investigate cytokine gene expression in the skin and peripheral blood of atopic dermatitis (AD) patients and healthy individuals. Methods. Samples of skin and peripheral blood from 48 severe AD patients SCORAD (Scoring Atopic Dermatitis) 78,5 [57; 89], IGA (Investigators Global Assessment) 4,2 [3,9; 4,7]) at the age of 17 to 45 years and 20 healthy donors aged from 19 to 32 years were analyzed for gene expression of cytokines using real time reverse transcription polymerase chain reaction (RT-PCR). Results. In the skin of patients with AD, a significant increase of the level of gene expression was observed for interleukin IL2R (interleukin) (р=0,0023), IL5 (р=0,002), IL6 (р=0,0023), IL8 (р=0,01), IL12β (р=0,0023), IL10 (р=0,0023), IL23 (р=0,002), IL29 (р=0,0023), and TGFβ (transforming growth factor) (p=0,0023) as compared to healthy individuals. In contrast, no difference between AD patients and healthy donors was detected with respect to cytokine gene expression in the peripheral blood. Conclusions. Activity of IL-2R, IL-8, IL-12β, IL-23, IL-29, and TGFβ that are markers of chronic inflammation and Th1 immune response in severe AD and IL-5, IL-10 that are anti-inflammatory cytokines and markers of Th2 response was predominant in the skin but not in the blood of AD patients.

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