Expression profiles of tyrosine metabolic pathway genes and functional analysis of DOPA decarboxylase in puparium tanning of Bactrocera dorsalis ( Hendel )

2021 ◽  
Author(s):  
Er‐Hu Chen ◽  
Qiu‐Li Hou ◽  
Wei Dou ◽  
Pei‐Jin Yang ◽  
Jin‐Jun Wang
Keyword(s):  
Functional Analysis ◽  
Metabolic Pathway ◽  
Expression Profiles ◽  
Bactrocera Dorsalis ◽  
Dopa Decarboxylase

scholarly journals Differential expression profiles and functional analysis of circular RNAs in children with fulminant myocarditis

Epigenomics ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1129-1141 ◽  
Author(s):  
Li Zhang ◽  
Bo Han ◽  
Jing Wang ◽  
Qingqing Liu ◽  
Yaru Kong ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Healthy Volunteers ◽  
Differential Expression ◽  
Expression Profiles ◽  
Interaction Network ◽  
Fulminant Myocarditis ◽  
Circular Rnas ◽  
Biological Functions ◽  
Microarray Experiments ◽  
Network Building

Aim: To assess differential expression profiles of circular RNAs (circRNAs) and explore their possible functions in children with fulminant myocarditis. Materials & methods: circRNA microarray experiments were carried out for determining differential expression profiles of circRNAs in three children with fulminant myocarditis and three healthy volunteers. Functional analysis and circRNA–miRNA–mRNA interaction network building were conducted to study biological functions. Results: This work identified 2281 upregulated and 892 downregulated circRNAs. Further assessment confirmed hsa_circ_0071542 upregulation (2.5-fold) in fulminant myocarditis. Functional analysis demonstrated the differentially expressed circRNAs mainly contributed to inflammation and immunity. Conclusion: circRNAs might have substantial roles in pediatric fulminant myocarditis, and hsa_circ_0071542 could serve as a promising biomarker.

scholarly journals The transformer-2 and fruitless characterisation with developmental expression profiles of sex-determining genes in Bactrocera dorsalis and B. correcta

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Kamoltip Laohakieat ◽  
Siriwan Isasawin ◽  
Sujinda Thanaphum
Keyword(s):  
Splice Variants ◽  
Expression Profiles ◽  
Bactrocera Dorsalis ◽  
Developmental Expression ◽  
Alternative Promoters ◽  
Downstream Effector ◽  
Effector Genes ◽  
Early Embryos ◽  
Alternatively Spliced ◽  
Tephritid Fruit Flies

Abstract Sex determination in tephritid fruit flies involves a signaling cascade of alternatively spliced genes. The Transformer (TRA) and Transformer-2 (TRA-2) complex establishes an autoregulatory loop switching sex-specific splicing of tra pre-mRNA in females. The TRA/TRA-2 complex also regulates the sex-specific splicing of downstream effector genes, doublesex (dsx) and fruitless (fru). In Ceratitis capitata, a Maleness-on the-Y (MoY) gene modulates sex-specifically spliced Cctra pre-mRNA and results in the breakdown of the Cctra autoregulatory loop in males. In this study, the tra-2 and fru genes were characterised in two key pests, Bactrocera dorsalis and B. correcta. The tra-2 genes showed high degrees of conservation among tephritids. The complex gene organisation for each of Bdfru and Bcfru were identified. There are sex-specific and non sex-specific transcripts generated by alternative promoters as found in Drosophila melanogaster and other insects. RNAi knockdown of Bdtra transcripts showed that BdTRA controls the sex-specific splicing of Bddsx and Bdfru pre-mRNAs. Developmental expression analysis shows that multiple splice variants of Bdtra and Bctra RNAs are present before and during cellular blastoderm formation and that the mature sex-specific variants become fixed later in embryogenesis. Furthermore, the BddsxM splice variants are found in early embryos at the beginning of gastulation, but BdfruM does not appear until the larval stage. We proposed that the zygotic tra loop is initiated in both female and male embryos before becoming automatised or abolished by MoY, respectively.

scholarly journals In vivo functional analysis of L-rhamnose metabolic pathway in Aspergillus niger: a tool to identify the potential inducer of RhaR

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Claire Khosravi ◽  
Roland Sándor Kun ◽  
Jaap Visser ◽  
María Victoria Aguilar-Pontes ◽  
Ronald P. de Vries ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Aspergillus Niger ◽  
Metabolic Pathway

Molecular characterization and functional analysis of BdFoxO gene in the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae)

Gene ◽  
2016 ◽  
Vol 578 (2) ◽  
pp. 219-224 ◽  
Author(s):  
Yi-Bei Wu ◽  
Wen-Jia Yang ◽  
Yi-Fei Xie ◽  
Kang-Kang Xu ◽  
Yi Tian ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Molecular Characterization ◽  
Fruit Fly ◽  
Bactrocera Dorsalis ◽  
Oriental Fruit Fly

scholarly journals Identification, Classification, and Functional Analysis of AP2/ERF Family Genes in the Desert Moss Bryum argenteum

2018 ◽  
Vol 19 (11) ◽  
pp. 3637 ◽  
Author(s):  
Xiaoshuang Li ◽  
Bei Gao ◽  
Daoyuan Zhang ◽  
Yuqing Liang ◽  
Xiaojie Liu ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Molecular Mechanisms ◽  
Expression Profiles ◽  
Abiotic Stress Tolerance ◽  
Plant Responses ◽  
Rna Seq ◽  
Bryum Argenteum ◽  
Erf Family ◽  
Stress Related Genes ◽  
Rehydration Process

Bryum argenteum is a desert moss which shows tolerance to the desert environment and is emerging as a good plant material for identification of stress-related genes. AP2/ERF transcription factor family plays important roles in plant responses to biotic and abiotic stresses. AP2/ERF genes have been identified and extensively studied in many plants, while they are rarely studied in moss. In the present study, we identified 83 AP2/ERF genes based on the comprehensive dehydrationrehydration transcriptomic atlas of B. argenteum. BaAP2/ERF genes can be classified into five families, including 11 AP2s, 43 DREBs, 26 ERFs, 1 RAV, and 2 Soloists. RNA-seq data showed that 83 BaAP2/ERFs exhibited elevated transcript abundances during dehydration–rehydration process. We used RT-qPCR to validate the expression profiles of 12 representative BaAP2/ERFs and confirmed the expression trends using RNA-seq data. Eight out of 12 BaAP2/ERFs demonstrated transactivation activities. Seven BaAP2/ERFs enhanced salt and osmotic stress tolerances of yeast. This is the first study to provide detailed information on the identification, classification, and functional analysis of the AP2/ERFs in B. argenteum. This study will lay the foundation for the further functional analysis of these genes in plants, as well as provide greater insights into the molecular mechanisms of abiotic stress tolerance of B. argenteum.

Molecular Characterization and Transcriptional Expression Analysis of ABC Transporter H Subfamily Genes in the Oriental Fruit Fly

2021 ◽  
Author(s):  
Wang He ◽  
Dan-Dan Wei ◽  
Hui-Qian Xu ◽  
Yang Yang ◽  
Ze-Qing Miao ◽  
...  
Keyword(s):  
Abc Transporters ◽  
Fruits And Vegetables ◽  
Developmental Stages ◽  
Expression Profiles ◽  
Extreme Temperature ◽  
Fruit Fly ◽  
Bactrocera Dorsalis ◽  
Mrna Levels ◽  
Oriental Fruit Fly ◽  
Starvation And Desiccation

Abstract The oriental fruit fly, Bactrocera dorsalis Hendel (Diptera: Tephretidae), is a serious pest of fruits and vegetables and has developed high levels of insecticide resistance. ATP-binding cassette transporter genes (ABC transporters) are involved in mediating the energy-driven transport of many substances across membranes and are closely associated with development and insecticide detoxification. In this study, three ABC transporters in the H subfamily were identified, and the possible roles of these genes in B. dorsalis are discussed. Bioinformatics analysis revealed that those genes are conserved, typical of half-transporters. The expression profiles of BdABCH genes (BdABCHs) in the developmental stages, tissues, and following insecticide exposure, extreme temperature, warm- and cold-acclimated strain, starvation, and desiccation stress were determined by quantitative real-time PCR. Expression of BdABCHs can be detected in various tissues and in different developmental stages. They were most highly expressed in the hindgut and in newly emerged adults. The mRNA levels of BdABCHs in males (including most tissues and body segments) were higher than in females. The expression of BdABCH1 was significantly upregulated 3.8-fold in the cold-acclimated strain, and was significantly upregulated by 1.9-, 3.8- and 4.1-fold in the 0°C, starvation, and desiccation treatments, respectively. Treatment with malathion and avermectin at LD20 and LD30 concentrations produced no obvious changes in the levels of BdABCHs. BdABCHs may be involved in the transport of related hormones during eclosion, as well as water and inorganic salts. BdABCH1 also demonstrated that it is related to the ability to cope with adverse environments.

Functional analysis of muscle gene expression profiles associated with tenderness and intramuscular fat content in pork

Meat Science ◽  
2012 ◽  
Vol 92 (4) ◽  
pp. 440-450 ◽  
Author(s):  
Ruth M. Hamill ◽  
Jean McBryan ◽  
Chris McGee ◽  
Anne Maria Mullen ◽  
Torres Sweeney ◽  
...  
Keyword(s):  
Gene Expression ◽  
Functional Analysis ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Muscle Gene Expression ◽  
Muscle Gene ◽  
Intramuscular Fat Content
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