Efficient Gene Delivery to Mesenchymal Stem Cells by an Ethylenediamine-Modified Polysaccharide from Mulberry Leaves

The cell-based approach in gene therapy arises as a promising strategy to provide safe, targeted, and efficient gene delivery. Owing to their unique features, as homing and tumor-tropism, mesenchymal stem cells (MSCs) have recently been introduced as an encouraging vehicle in gene therapy. Nevertheless, non-viral transfer of nucleic acids into MSCs remains limited due to various factors related to the main stakeholders of the process (e.g., nucleic acids, carriers, or cells). In this review, we have summarized the main types of nucleic acids used to transfect MSCs, the pros and cons, and applications of each. Then, we have emphasized on the most efficient lipid-based carriers for nucleic acids to MSCs, their main features, and some of their applications. While a myriad of studies have demonstrated the therapeutic potential for engineered MSCs therapy in various illnesses, optimization for clinical use is an ongoing challenge. On the way of improvement, genetically modified MSCs have been combined with various novel techniques and tools (e.g., exosomes, spheroids, 3D-Bioprinting, etc.,) aiming for more efficient and safe applications in biomedicine.

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Comparative study of BMP-2 gene delivery to Human adipose tissue-derived mesenchymal stem cells with Turbofect and Polyethylenimine

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/677/4/042024 ◽

2021 ◽

Vol 677 (4) ◽

pp. 042024

Author(s):

I A Nedorubova ◽

T B Bukharova ◽

A V Vasilyev ◽

M A Syachina ◽

D V Goldshtein ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Adipose Tissue ◽

Gene Delivery ◽

Comparative Study ◽

Human Adipose Tissue

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Mesenchymal stem cells in drug/gene delivery: implications for cell therapy

Therapeutic Delivery ◽

10.4155/tde.12.69 ◽

2012 ◽

Vol 3 (8) ◽

pp. 997-1004 ◽

Cited By ~ 44

Author(s):

Steven J Greco ◽

Pranela Rameshwar

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Gene Delivery ◽

Cell Therapy

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Functionalized Scaffold for in Situ Efficient Gene Transfection of Mesenchymal Stem Cells Spheroids toward Chondrogenesis

ACS Applied Materials & Interfaces ◽

10.1021/acsami.8b12268 ◽

2018 ◽

Vol 10 (40) ◽

pp. 33993-34004 ◽

Cited By ~ 10

Author(s):

Kunxi Zhang ◽

Haowei Fang ◽

Yechi Qin ◽

Lili Zhang ◽

Jingbo Yin

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Gene Transfection ◽

Efficient Gene

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Evaluation of low molecular weight polyethylenimine-introduced chitosan for gene delivery to mesenchymal stem cells

Materials Express ◽

10.1166/mex.2020.1780 ◽

2020 ◽

Vol 10 (7) ◽

pp. 1170-1176

Author(s):

Minchen Liu ◽

Yulan Hu ◽

Yi Feng

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Gene Delivery ◽

Transfection Efficiency ◽

A549 Cells ◽

Safety Evaluation ◽

Physicochemical Characteristics ◽

Hatching Rate

This study aimed to examine the transfection ability of polyethylenimine (PEI) (1800 Da)-grafted chitosan (10 kDa) (CP), a newly synthesized PEI derivative, in mesenchymal stem cells (MSCs). The safety evaluation of the complex/DNA was studied in vitro and in vivo. In addition, CP/pGL3 was applied to investigate the effects of transfection efficiency. In this study, CP/DNA can be formed with compatible physicochemical characteristics for gene delivery. CP cytotoxicity decreased in A549 cells. Moreover, a zebrafish embryo model was used for evaluating the safety in vivo. Compared to the PEI (25 kDa) group, the zebrafish hatching rate increased and the mortality rate decreased in the CP/DNA group, which provided an indication of the safety of CP. In comparison with chitosan (100 kDa)-PEI (1200 Da), CP's transfection efficiency was higher in both A549 cells and MSCs. This study aimed to lay the foundation for further applications of CP in gene delivery. Therefore, further gene therapy investigations of CP by using MSCs need to be performed.

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