Enhanced tissue remodelling efficacy of adipose‐derived mesenchymal stem cells using injectable matrices in radiation‐damaged salivary gland model

Our study assess whether exosomes derived from bone marrow mesenchymal stem cells (BM-MSC) ameliorates diabetic salivary gland complications. 10 SD rats were assigned into diabetes group I and exosome treatment group II. Diabetic rats were induced by streptozotocin (STZ) and injected with DMSO or exosomes through tail vein followed by collection of submandibular salivary gland samples for histological analysis and TGFβ, Smad2 and Smad3 level by PCR, saliva IgA and serum amylase level. Compared with control mice, exosome treatment mice showed less fibrosis of the submandibular salivary glands and duct components with a more complete structure. Exosome treatment inhibited TGFβ, Smad2 and Smad3 level to reduce diabetic salivary gland complications, effectively decreased blood sugar level, improved salivary glands function with significantly reduced serum amylase and salivary IgA levels. In conclusion, BM-MSC-derived exosomes may be a new therapeutic strategy for treating diabetic salivary gland complications.

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Intraglandular transplantation of bone marrow-derived clonal mesenchymal stem cells for amelioration of post-irradiation salivary gland damage

Oral Oncology ◽

10.1016/j.oraloncology.2012.08.010 ◽

2013 ◽

Vol 49 (2) ◽

pp. 136-143 ◽

Cited By ~ 62

Author(s):

Jae-Yol Lim ◽

TacGhee Yi ◽

Jeong-Seok Choi ◽

Yun Ho Jang ◽

Songyi Lee ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Salivary Gland ◽

Post Irradiation

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Adipose-derived mesenchymal stem cells regenerate radioiodine-induced salivary gland damage in a murine model

Scientific Reports ◽

10.1038/s41598-019-51775-9 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 4

Author(s):

Ji Won Kim ◽

Jeong Mi Kim ◽

Mi Eun Choi ◽

Seok-Ki Kim ◽

Young-Mo Kim ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Salivary Gland ◽

Murine Model ◽

Sham Group ◽

Apoptotic Cell ◽

Salivary Flow ◽

Treated Group ◽

The Body ◽

Oral Infections

Abstract After radioiodine (RI) therapy, patients with thyroid cancer frequently suffer from painful salivary gland (SG) swelling, xerostomia, taste alterations, and oral infections. This study was aimed to determine whether adipose-derived mesenchymal stem cells (AdMSCs) might restore RI-induced SG dysfunction in a murine model. Forty -five mice were divided into three groups; a PBS sham group, a RI+ PBS sham group (0.01 mCi/g mouse, orally), and an RI+AdMSCs (1 × 105 cells/150 uL, intraglandular injection on experimental day 28) treated group. At 16 weeks after RI treatment, body weights, SG weight, salivary flow rates (SFRs), and salivary lag times were measured. Morphologic and histologic examinations and immunohistochemistry (IHC) were performed and the activities of amylase and EGF in saliva were also measured. Changes in salivary 99mTc pertechnetate excretion were followed by SPECT and TUNEL assays were performed. The body and SG weights were similar in the AdMSCs and sham groups. Hematoxylin and eosin staining revealed the AdMSCs group had more mucin-containing acini than the RI group. Furthermore, AdMSCs treatment resulted in tissue remodeling and elevated expressions of epithelial (AQP5) and endothelial (CD31) markers, and increased SFRs. The activities of amylase and EGF were higher in the AdMSCs group than in the RI treated group. 99mTc pertechnetate excretions were similar in the AdMSCs and sham group. Also, TUNEL positive apoptotic cell numbers were less in the AdMSCs group than in the RI group. Local delivery of AdMSCs might regenerate SG damage induced by RI.

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Mesenchymal stem cells enhance AQP1 expression in the sublingual salivary gland of ovariectomized menopausal rat model

Annals of Anatomy - Anatomischer Anzeiger ◽

10.1016/j.aanat.2021.151714 ◽

2021 ◽

pp. 151714

Author(s):

Nesma I. El-naseery ◽

Yaser H.A. Elewa ◽

Mona A.A. Arafa ◽

W.S. Sabbah ◽

Arigue A. Dessouky

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Salivary Gland ◽

Rat Model ◽

Aqp1 Expression

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Mesenchymal stem cells in rheumatology: a regenerative approach to joint repair

Clinical Science ◽

10.1042/cs20070126 ◽

2007 ◽

Vol 113 (8) ◽

pp. 339-348 ◽

Cited By ~ 33

Author(s):

Cosimo De Bari ◽

Francesco Dell'Accio

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Stem Cell ◽

Mesenchymal Stem Cell ◽

Cell Biology ◽

Current Knowledge ◽

Treatment Options ◽

Joint Destruction ◽

Tissue Remodelling ◽

Regenerative Approach

The advent of biologics in rheumatology has considerably changed the evolution and prognosis of chronic inflammatory arthritis. The success of these new treatments has contributed to steering more attention to research focussed on repair and remodelling of joint tissues. Indeed, when the tissue damage is established, treatment options are very limited and the risk of progression towards joint destruction and failure remains high. Increasing evidence indicates that mesenchymal stem cells persist postnatally within joint tissues. It is postulated that they would function to safeguard joint homoeostasis and guarantee tissue remodelling and repair throughout life. Alterations in mesenchymal stem cell biology in arthritis have indeed been reported but a causal relationship has not been demonstrated, mainly because our current knowledge of mesenchymal stem cell niches and functions within the joint in health and disease is very limited. Nonetheless, mesenchymal stem cell technologies have attracted the attention of the biomedical research community as very promising tools to achieve the repair of joint tissues such as articular cartilage, subchondral bone, menisci and tendons. This review will outline stem-cell-mediated strategies for the repair of joint tissues, spanning from the use of expanded mesenchymal stem cell populations to therapeutic targeting of endogenous stem cells, resident in their native tissues, and related reparative signals in traumatic, degenerative and inflammatory joint disorders.

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Muscle Derived Mesenchymal Stem Cells Inhibit the Activity of the Free and the Neutrophil Extracellular Trap (NET)-Bond Myeloperoxidase

Cells ◽

10.3390/cells10123486 ◽

2021 ◽

Vol 10 (12) ◽

pp. 3486

Author(s):

Thierry Franck ◽

Justine Ceusters ◽

Hélène Graide ◽

Ange Mouithys-Mickalad ◽

Didier Serteyn

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Immune Modulation ◽

Tissue Injury ◽

Specific Treatment ◽

Neutrophil Extracellular Trap ◽

Ros Production ◽

Tissue Remodelling ◽

Direct Role ◽

Extracellular Trap

Mesenchymal stem cells (MSCs) are known to migrate to tissue injury sites to participate in immune modulation, tissue remodelling and wound healing, reducing tissue damage. Upon neutrophil activation, there is a release of myeloperoxidase (MPO), an oxidant enzyme. But little is known about the direct role of MSCs on MPO activity. The aim of this study was to investigate the effect of equine mesenchymal stem cells derived from muscle microinvasive biopsy (mdMSC) on the oxidant response of neutrophils and particularly on the activity of the myeloperoxidase released by stimulated equine neutrophils. After specific treatment (trypsin and washings in phosphate buffer saline), the mdMSCs were exposed to isolated neutrophils. The effect of the suspended mdMSCs was studied on the ROS production and the release of total and active MPO by stimulated neutrophils and specifically on the activity of MPO in a neutrophil-free model. Additionally, we developed a model combining adherent mdMSCs with neutrophils to study total and active MPO from the neutrophil extracellular trap (NET). Our results show that mdMSCs inhibited the ROS production, the activity of MPO released by stimulated neutrophils and the activity of MPO bound to the NET. Moreover, the co-incubation of mdMSCs directly with MPO results in a strong inhibition of the peroxidase activity of MPO, probably by affecting the active site of the enzyme. We confirm the strong potential of mdMSCs to lower the oxidant response of neutrophils. The novelty of our study is an evident inhibition of the activity of MPO by MSCs. The results indicated a new potential therapeutic approach of mdMSCs in the inhibition of MPO, which is considered as a pro-oxidant actor in numerous chronic and acute inflammatory pathologies.

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A Synopsis of Signaling Crosstalk of Pericytes and Endothelial Cells in Salivary Gland

Dentistry Journal ◽

10.3390/dj9120144 ◽

2021 ◽

Vol 9 (12) ◽

pp. 144

Author(s):

Ioana Cucu ◽

Mihnea Ioan Nicolescu

Keyword(s):

Stem Cells ◽

Endothelial Cells ◽

Mesenchymal Stem Cells ◽

Salivary Gland ◽

Building Blocks ◽

Myoepithelial Cells ◽

Molecular Networks ◽

Signaling Cascades ◽

Cellular Organization ◽

Signaling Crosstalk

The salivary gland (SG) microvasculature constitutes a dynamic cellular organization instrumental to preserving tissue stability and homeostasis. The interplay between pericytes (PCs) and endothelial cells (ECs) culminates as a key ingredient that coordinates the development, maturation, and integrity of vessel building blocks. PCs, as a variety of mesenchymal stem cells, enthrall in the field of regenerative medicine, supporting the notion of regeneration and repair. PC-EC interconnections are pivotal in the kinetic and intricate process of angiogenesis during both embryological and post-natal development. The disruption of this complex interlinkage corresponds to SG pathogenesis, including inflammation, autoimmune disorders (Sjögren’s syndrome), and tumorigenesis. Here, we provided a global portrayal of major signaling pathways between PCs and ECs that cooperate to enhance vascular steadiness through the synergistic interchange. Additionally, we delineated how the crosstalk among molecular networks affiliate to contribute to a malignant context. Additionally, within SG microarchitecture, telocytes and myoepithelial cells assemble a labyrinthine companionship, which together with PCs appear to synchronize the regenerative potential of parenchymal constituents. By underscoring the intricacy of signaling cascades within cellular latticework, this review sketched a perceptive basis for target-selective drugs to safeguard SG function.

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