Magnesium lithospermate B supplementation improved prenatal Bisphenol A exposure‐induced metabolic abnormalities in male offspring

2021 ◽  
Author(s):  
Wei‐Chi Huang ◽  
Keng‐Ying Liao ◽  
Sheng‐Kuo Hsieh ◽  
Ping‐Ho Pan ◽  
Yu‐Hsiang Kuan ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Male Offspring ◽  
Metabolic Abnormalities ◽  
Magnesium Lithospermate B

scholarly journals Bile Acids and Tryptophan Metabolism Are Novel Pathways Involved in Metabolic Abnormalities in BPA-Exposed Pregnant Mice and Male Offspring

Endocrinology ◽  
2017 ◽  
Vol 158 (8) ◽  
pp. 2533-2542 ◽  
Author(s):  
Martha Susiarjo ◽  
Frances Xin ◽  
Martha Stefaniak ◽  
Clementina Mesaros ◽  
Rebecca A Simmons ◽  
...  
Keyword(s):  
Bile Acids ◽  
Tryptophan Metabolism ◽  
Male Offspring ◽  
Metabolic Abnormalities ◽  
Pregnant Mice

scholarly journals Magnesium Lithospermate B Attenuates High-Fat Diet-Induced Muscle Atrophy in C57BL/6J Mice

Nutrients ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 104
Author(s):  
Tsun-Li Cheng ◽  
Zi-Yun Lin ◽  
Keng-Ying Liao ◽  
Wei-Chi Huang ◽  
Cian-Fen Jhuo ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Atrophy ◽  
High Fat Diet ◽  
Skeletal Muscle Atrophy ◽  
Metabolic Abnormalities ◽  
High Fat ◽  
E3 Ligases ◽  
Beneficial Effects ◽  
Magnesium Lithospermate B ◽  
Tnf Α

Magnesium lithospermate B (MLB) is a primary hydrophilic component of Danshen, the dried root of Salvia miltiorrhiza used in traditional medicine, and its beneficial effects on obesity-associated metabolic abnormalities were reported in our previous study. The present study investigated the anti-muscle atrophy potential of MLB in mice with high-fat diet (HFD)-induced obesity. In addition to metabolic abnormalities, the HFD mice had a net loss of skeletal muscle weight and muscle fibers and high levels of muscle-specific ubiquitin E3 ligases, namely the muscle atrophy F-box (MAFbx) and muscle RING finger protein 1 (MuRF-1). MLB supplementation alleviated those health concerns. Parallel changes were revealed in high circulating tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), skeletal TNF receptor I (TNFRI), nuclear factor-kappa light chain enhancer of activated B cells (NF-κB), p65 phosphorylation, and Forkhead box protein O1 (FoxO1) as well as low skeletal phosphoinositide 3-kinase (PI3K) and protein kinase B (Akt) phosphorylation. The study revealed that MLB prevented obesity-associated skeletal muscle atrophy, likely through the inhibition of MAFbx/MuRF-1-mediated muscular degradation. The activation of the PI3K-Akt-FoxO1 pathway and inhibition of the TNF-α/TNFRI/NF-κB pathway were assumed to be beneficial effects of MLB.

666: Maternal bisphenol a (BPA) exposure programs male offspring hepatic insulin signaling protein expression

2015 ◽  
Vol 212 (1) ◽  
pp. S327-S328
Author(s):  
Kristina Galyon ◽  
Farnoosh Farshidi ◽  
Michael Ross ◽  
Mina Desai ◽  
Juanita Jellyman
Keyword(s):  
Bisphenol A ◽  
Protein Expression ◽  
Insulin Signaling ◽  
Male Offspring ◽  
Signaling Protein ◽  
Hepatic Insulin Signaling

Paternal bisphenol a diet changes prefrontal cortex proteome and provokes behavioral dysfunction in male offspring

Chemosphere ◽  
2017 ◽  
Vol 184 ◽  
pp. 720-729 ◽  
Author(s):  
Guangying Luo ◽  
Ruifen Wei ◽  
Shaolin Wang ◽  
Jundong Wang
Keyword(s):  
Prefrontal Cortex ◽  
Bisphenol A ◽  
Male Offspring ◽  
Behavioral Dysfunction

scholarly journals Bisphenol A Exposure Disrupts Metabolic Health Across Multiple Generations in the Mouse

Endocrinology ◽  
2015 ◽  
Vol 156 (6) ◽  
pp. 2049-2058 ◽  
Author(s):  
Martha Susiarjo ◽  
Frances Xin ◽  
Amita Bansal ◽  
Martha Stefaniak ◽  
Changhong Li ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Bisphenol A ◽  
Glucose Homeostasis ◽  
Early Life ◽  
Metabolic Health ◽  
Male Offspring ◽  
Differentially Methylated Region ◽  
Developmental Defects ◽  
Metabolic Phenotypes ◽  
Multiple Generations

Abstract Accumulating evidence has suggested that a suboptimal early life environment produces multigenerational developmental defects. A proposed mechanism is stable inheritance of DNA methylation. Here we show that maternal bisphenol A (BPA) exposure in C57BL/6 mice produces multigenerational metabolic phenotypes in their offspring. Using various methods including dual-energy X-ray absorptiometry analyses, glucose tolerance tests, and perifusion islet studies, we showed that exposure to 10 μg/kg/d and 10 mg/kg/d BPA in pregnant F0 mice was associated with higher body fat and perturbed glucose homeostasis in F1 and F2 male offspring but not female offspring. To provide insight into the mechanism of the multigenerational metabolic abnormalities, we investigated the maternal metabolic milieu and inheritance of DNA methylation across generations. We showed that maternal glucose homeostasis during pregnancy was altered in the F0 but not F1 female mice. The results suggested that a compromised maternal metabolic milieu may play a role in the health of the F1 offspring but cannot account for all of the observed multigenerational phenotypes. We further demonstrated that the metabolic phenotypes in the F1 and F2 BPA male offspring were linked to fetal overexpression of the imprinted Igf2 gene and increased DNA methylation at the Igf2 differentially methylated region 1. Studies in H19Δ3.8/+ mouse mutants supported the role of fetal Igf2 overexpression in altered adult glucose homeostasis. We conclude that early life BPA exposure at representative human exposure levels can perturb metabolic health across multiple generations in the mouse through stable inheritance of DNA methylation changes at the Igf2 locus.

Maternal exposure to low doses of bisphenol A affects learning and memory in male rat offspring with abnormal N-methyl-d-aspartate receptors in the hippocampus

2021 ◽  
pp. 074823372098462
Author(s):  
Chong Wang ◽  
Yao Shu ◽  
Li Xu ◽  
Qiling Liu ◽  
Bei Zhang ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Learning And Memory ◽  
Maternal Exposure ◽  
Male Offspring ◽  
Male Rat ◽  
Control Group ◽  
Sprague Dawley ◽  
Expression Levels ◽  
Rat Offspring ◽  
Sprague Dawley Rats

Bisphenol A (BPA), a component of polycarbonate and epoxy resins, has been reported to induce learning and memory deficits. However, the mechanisms have not been fully elucidated. Growing evidence has suggested that N-methyl-d-aspartate receptors (NMDARs) are involved in cognitive impairments. In this study, BPA was administered to female Sprague–Dawley rats (six per dose group) at concentrations of 0 (control), 4, 40, and 400 μg/kg·body weight/day from gestation day 1 through lactation day 21. Spatial learning was evaluated using the Morris water maze on postnatal day 22. Expression levels of NMDARs were determined using real-time polymerase chain reaction and Western blot. The results showed that male offspring exposed to BPA exhibited increased latency in reaching the platform and reduced time in the target quadrant, and the number of crossing the platform was less, as compared with the control group. The mRNA and protein expression levels of NMDARs in the hippocampus were significantly downregulated when compared with the control group of male offspring. The data showed that maternal exposure to BPA at low dosage can cause cognitive deficits in male rat offspring, probably due to a decrease in NMDARs in the hippocampus.

scholarly journals EPIGENOMIC EFFECTS OF EXPOSURE OF PREGNANT RATS TO LOW DOSE OF BISPHENOL A REGARDING THE NEUROENDOCRINE SYSTEM OF THE MALE F1 GENERATION (short communication)

2021 ◽  
pp. 23-28
Author(s):  
Alexander Reznikov ◽  
Olha Sachynska ◽  
Аnna Lymareva ◽  
Lyubov Polyakova
Keyword(s):  
Sexual Behavior ◽  
Bisphenol A ◽  
Low Dose ◽  
Male Offspring ◽  
Male Rats ◽  
Normal Male ◽  
Potential Hazard ◽  
Preoptic Nucleus ◽  
Female Behavior ◽  
Medial Preoptic Nucleus

Aim: To study the long-term effects of exposure of pregnant Wistar rats to low dose of bisphenol A (BPA) by measuring to the level of steroid hormones and sexual behavior of adult male offspring of the first generation. Material and research methods: BPA as part of the Dorfman gel was gavaged during the last week of pregnancy, when androgen-dependent sexual brain differentiation occurs, in a daily dose of 25 mcg/kg b.w. (threshold teratogenic dose). Male sexual behavior was evaluated by proceptive reactions, the duration of latent and refractory periods, the number of mounts, intromissions and ejaculations in the presence of a receptive female. Female sexual behavior was assessed by lordosis reactions of orchidectomized and activated by the introduction of estradiol and progesterone males in the presence of a normal male. A neuromorphological analysis of the sex-dimorphic area of the brain, the medial preoptic nucleus of the hypothalamus, was performed by histological examination and karyometry of neurons. Results: Prenatally administered BPA caused a very slight increase in the anogenital distance in newborn animals and did not affect the terms of puberty. The levels of testosterone and corticosterone in the blood plasma of males of 6 months of age did not differ from the control indices. At 10 months of age, all experimental males showed sharply weakened sexual motivation for mating with females, and in 4 from 5 animals, copulative components of sexual behavior were absent. There was no ejaculations in the 5th male as well, while numbers of the mounts without intromissions and ones with intromissions significantly reduced. In the BPA group, all descendants showed active female behavior in the presence of a normal male, which manifested in lordosis reactions and a high lordosis index. According to the histological study of medial preoptic nucleus, the activity of neurocytes in the male offspring of BPA-exposed females was significantly reduced, and their nuclei volume distribution was some different from the control. Conclusions: The data obtained indicate epigenetic disorders of the sexual brain differentiation program due to the prenatal exposure to BPA in dose that does not cause significant teratogenic effects. This should be taken into account when evaluating the potential hazard of BPA for reproductive health. Key words: bisphenol A, prenatal effect, male rats, sexual behavior, corticosterone, testosterone.

Parental Exposure to Bisphenol A During Pregnancy and the Anogenital Distance of Male Offspring

Epidemiology ◽  
2011 ◽  
Vol 22 ◽  
pp. S242 ◽  
Author(s):  
Maohua Miao ◽  
Wei Yuan ◽  
Yonghua He ◽  
Zhijun Zhou ◽  
Jintao Wang ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Male Offspring ◽  
Anogenital Distance ◽  
Parental Exposure

Perinatal exposure to a low dose of bisphenol-A advances pubertal spermatogenesis of F1 adolescent male rats

2020 ◽  
Author(s):  
Yinyang Bai ◽  
Fang Xiong ◽  
Yun Zhang ◽  
Jie Chen ◽  
Lishuang Xu ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Plasma Levels ◽  
Low Dose ◽  
Male Offspring ◽  
Female Rats ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Perinatal Exposure ◽  
Significant Difference ◽  
The Impact

Abstract Background To investigate the impact of perinatal exposure to a low dose of bisphenol A (BPA) on spermatogenesis in male rats and the underlying mechanism. Methods Female rats were injected subcutaneously with 2 µg BPA/kg/day from gestation day 10 through lactation day 7. The spermatogenesis and expression of key regulatory genes in the testes as well as the central modulators of the hypothalamic-pituitary-gonadal axis were determined in male offspring on postnatal day 18, 21, and 24 (PND18, 21, and 24). Results 1) Perinatal BPA exposure led to an increase in the weight of body and testis in PND21-24 male offspring. The seminiferous tubular diameter and the number of round spermatids were significantly increased in PND21 BPA-rats, while the volumes of the Sertoli cells, spermatogonia and spermatocytes were not significantly altered. 2) Compared to the control rats, the expression levels of key meiotic regulators such as cyclinA1, c-jun and c-fos in the seminiferous tubules were significantly elevated in PND21 BPA-rats. 3) The plasma levels of FSH and LH (PND21 and PND24) as well as the frequency of pulsatile LH secretion (PND21) were significantly increased in BPA-rats, although the plasma levels of testosterone and estrogen showed no significant difference between the two groups. 4) In comparison with control rats, the levels of GnRH mRNA in the preoptic area (POA) and kiss1 mRNA in arcuate nucleus (ARC) were significantly increased in the BPA-rats, whereas the level of ERα mRNA in ARC was decreased, although the number of GnRH-positive cells and ARC kisspeptin-positive cells were unchanged. Interestingly, neither the number of kisspeptin-positive cells nor the level of kiss1 mRNA in the anteroventral periventricular nucleus (AVPV) showed a difference between the two groups. Conclusion Perinatal exposed to a low dose of BPA leads to an increased meiosis of spermatocytes and promotes the spermatogenesis in male offspring, most likely through activation of the hypothalamic-pituitary-gonadal axis.

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