Investigating Internalization and Intracellular Trafficking of GPCRs: New Techniques and Real-Time Experimental Approaches

Author(s):  
Simon R. Foster ◽  
Hans Bräuner-Osborne

2012 ◽  
Vol 594-597 ◽  
pp. 738-741 ◽  
Author(s):  
Yin Duan ◽  
Xing Hong Liu ◽  
Xiao Lin Chang

Main factors of the temperature control and crack prevention in arch dams are summarized. The Space-time Dynamic Control method in pipe cooling process and the Temperature Real-time Control and Decision Database System are introduced to help for temperature real-time control and rapid analysis. Successful application of these new techniques in the construction of Dagangshan arch dam indicates that the proposed method are of significant effectiveness on the temperature control and crack prevention, and have good application prospect in practical project.



2002 ◽  
Vol 20 (7) ◽  
pp. 1031-1038 ◽  
Author(s):  
A. H. Y. Chan ◽  
P. S. Cannon

Abstract. Space weather effects can strongly influence high-frequency (HF) communications by changing the ionospheric environment through which the radio waves propagate. Since many systems utilize HF communications, the ability to make real-time assessments of propagation conditions is an important part of space weather monitoring systems. In this paper, we present new techniques for measuring high-latitude HF communications link parameters using data from SuperDARN radars. These techniques use ground-scatter returns to define the variation in skip distance with frequency. From these data, the maximum usable frequency (MUF) as a function of range is determined and ionospheric critical frequencies are estimated. These calculations are made in near-real-time and the results are made available on the World Wide Web. F-region critical frequencies calculated using this method show good agreement with ionosonde data.Key words. Ionosphere (active experiments; instruments and techniques) – Radio science (ionospheric propagation)



2017 ◽  
Vol 9 (25) ◽  
pp. 21200-21208 ◽  
Author(s):  
Yuping Han ◽  
Xiaoming Li ◽  
Haobin Chen ◽  
Xingjie Hu ◽  
Yao Luo ◽  
...  


2020 ◽  
Vol 24 (5) ◽  
pp. 709-722
Author(s):  
Kieran Woodward ◽  
Eiman Kanjo ◽  
Andreas Oikonomou ◽  
Alan Chamberlain

Abstract In recent years, machine learning has developed rapidly, enabling the development of applications with high levels of recognition accuracy relating to the use of speech and images. However, other types of data to which these models can be applied have not yet been explored as thoroughly. Labelling is an indispensable stage of data pre-processing that can be particularly challenging, especially when applied to single or multi-model real-time sensor data collection approaches. Currently, real-time sensor data labelling is an unwieldy process, with a limited range of tools available and poor performance characteristics, which can lead to the performance of the machine learning models being compromised. In this paper, we introduce new techniques for labelling at the point of collection coupled with a pilot study and a systematic performance comparison of two popular types of deep neural networks running on five custom built devices and a comparative mobile app (68.5–89% accuracy within-device GRU model, 92.8% highest LSTM model accuracy). These devices are designed to enable real-time labelling with various buttons, slide potentiometer and force sensors. This exploratory work illustrates several key features that inform the design of data collection tools that can help researchers select and apply appropriate labelling techniques to their work. We also identify common bottlenecks in each architecture and provide field tested guidelines to assist in building adaptive, high-performance edge solutions.



2009 ◽  
Vol 83 (1) ◽  
pp. 111-120 ◽  
Author(s):  
Caterina Benzi ◽  
Chiara A. Bertolino ◽  
Ivana Miletto ◽  
Paola Ponzio ◽  
Claudia Barolo ◽  
...  




Nanoscale ◽  
2015 ◽  
Vol 7 (2) ◽  
pp. 597-612 ◽  
Author(s):  
Yajie Gao ◽  
Yanfang Li ◽  
Yushu Li ◽  
Lan Yuan ◽  
Yanxia Zhou ◽  
...  

To ensure that anticancer drugs could be delivered to tumor region and rapidly escape from endo/lysosomes, YPSMA-1-modified pH-sensitive polymeric micelles were designed and fabricated for targeted therapy of tumors.



2021 ◽  
Author(s):  
Junye Ge ◽  
Guangyuan Li ◽  
Haibo Zhang ◽  
Haiying Liu ◽  
Chuchu Qi ◽  
...  

Abstract The jump is one of the common stereotyped behavior in rodents. It is the natural state in some types of mice and also can be found in certain types of disease models, such as addiction. It is straightforward and easy to identify by the human eye in offline analysis. However, jumping is a short-lived act that happens immediately. It is difficult to be tagged in real-time by manual operation, which limits the detailed exploration of its neural mechanisms with the new techniques, such as fiber photometry recording or optogenetics. Here we introduced an arduino real-time jump tagging system (Art-JT system) to record the jump based on online monitoring the pressure changes of the floor in which the mouse is free exploring. Meanwhile, the Art-JT system can send the digital signal of the jump timing to the external device for tagging the events in the fiber photometry system or triggering the optogenetics laser. We tested it with the mice induced by Naloxone precipitated withdrawal jumping. The results showed that it could accurately record the jump events and provide several detailed parameters of the jump. Furthermore, it was easy and fast to get the GCaMP6 signal correlated with the jump in the medial prefrontal cortex and primary motor cortex by combining the Art-JT system and multichannel fiber photometry system. Our results suggested that the Art-JT system may be a powerful tool for recording and analyzing jumping efficiently and helping us to understand stereotyped behavior.



2019 ◽  
Vol IV (II) ◽  
pp. 1-6
Author(s):  
Mark Perkins

The huge proliferation of textual (and other data) in digital and organisational sources has led to new techniques of text analysis. The potential thereby unleashed may be underpinned by further theoretical developments to the theory of Discourse Stream Analysis (DSA) as presented here. These include the notion of change in the discourse stream in terms of discourse stream fronts, linguistic elements evolving in real time, and notions of time itself in terms of relative speed, subject orientation and perception. Big data has also given rise to fake news, the manipulation of messages on a large scale. Fake news is conveyed in fake discourse streams and has led to a new field of description and analysis.



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