In Vitro Approaches to Assessing the Toxicity of Quantum Dots

2014 ◽  
pp. 155-163 ◽  
Author(s):  
Ryan S. McMahan ◽  
Vivian Lee ◽  
William C. Parks ◽  
Terrance J. Kavanagh ◽  
David L. Eaton
Keyword(s):  
Quantum Dots

scholarly journals Effects of Doxorubicin Delivery by Nitrogen-Doped Graphene Quantum Dots on Cancer Cell Growth: Experimental Study and Mathematical Modeling

Nanomaterials ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 140
Author(s):  
Madison Frieler ◽  
Christine Pho ◽  
Bong Han Lee ◽  
Hana Dobrovolny ◽  
Giridhar R. Akkaraju ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cell Growth ◽  
Cancer Cell ◽  
Graphene Quantum Dots ◽  
Maximum Effect ◽  
Cancer Cell Growth ◽  
Nitrogen Doped ◽  
Nitrogen Doped Graphene ◽  
Doped Graphene

With 18 million new cases diagnosed each year worldwide, cancer strongly impacts both science and society. Current models of cancer cell growth and therapeutic efficacy in vitro are time-dependent and often do not consider the Emax value (the maximum reduction in the growth rate), leading to inconsistencies in the obtained IC50 (concentration of the drug at half maximum effect). In this work, we introduce a new dual experimental/modeling approach to model HeLa and MCF-7 cancer cell growth and assess the efficacy of doxorubicin chemotherapeutics, whether alone or delivered by novel nitrogen-doped graphene quantum dots (N-GQDs). These biocompatible/biodegradable nanoparticles were used for the first time in this work for the delivery and fluorescence tracking of doxorubicin, ultimately decreasing its IC50 by over 1.5 and allowing for the use of up to 10 times lower doses of the drug to achieve the same therapeutic effect. Based on the experimental in vitro studies with nanomaterial-delivered chemotherapy, we also developed a method of cancer cell growth modeling that (1) includes an Emax value, which is often not characterized, and (2), most importantly, is measurement time-independent. This will allow for the more consistent assessment of the efficiency of anti-cancer drugs and nanomaterial-delivered formulations, as well as efficacy improvements of nanomaterial delivery.

scholarly journals Synthesis and Characterization of Polyvinylpyrrolidone-Modified ZnO Quantum Dots and Their In Vitro Photodynamic Tumor Suppressive Action

2021 ◽  
Vol 22 (15) ◽  
pp. 8106
Author(s):  
Tianming Song ◽  
Yawei Qu ◽  
Zhe Ren ◽  
Shuang Yu ◽  
Mingjian Sun ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Photodynamic Therapy ◽  
Inhibitory Effect ◽  
Therapeutic Effects ◽  
In Vivo Experiments ◽  
Potential Applications ◽  
Zno Quantum Dots ◽  
Zno Qds

Despite the numerous available treatments for cancer, many patients succumb to side effects and reoccurrence. Zinc oxide (ZnO) quantum dots (QDs) are inexpensive inorganic nanomaterials with potential applications in photodynamic therapy. To verify the photoluminescence of ZnO QDs and determine their inhibitory effect on tumors, we synthesized and characterized ZnO QDs modified with polyvinylpyrrolidone. The photoluminescent properties and reactive oxygen species levels of these ZnO/PVP QDs were also measured. Finally, in vitro and in vivo experiments were performed to test their photodynamic therapeutic effects in SW480 cancer cells and female nude mice. Our results indicate that the ZnO QDs had good photoluminescence and exerted an obvious inhibitory effect on SW480 tumor cells. These findings illustrate the potential applications of ZnO QDs in the fields of photoluminescence and photodynamic therapy.

scholarly journals In vitro tracking of phospholipase A2 from snake venom conjugated with magic-sized quantum dots

2019 ◽  
Vol 122 ◽  
pp. 461-468 ◽  
Author(s):  
Edigar H.V. Dias ◽  
Déborah F.C. Pereira ◽  
Bruna B. de Sousa ◽  
Mariana S. Matias ◽  
Mayara R. de Queiroz ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Phospholipase A2 ◽  
Snake Venom

scholarly journals PEGylated Phospholipid Micelle-Encapsulated Near-Infrared PbS Quantum Dots for in vitro and in vivo Bioimaging

Theranostics ◽  
2012 ◽  
Vol 2 (7) ◽  
pp. 723-733 ◽  
Author(s):  
Rui Hu ◽  
Wing-Cheung Law ◽  
Guimiao Lin ◽  
Ling Ye ◽  
Jianwei Liu ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Near Infrared ◽  
Pbs Quantum Dots

scholarly journals Synthesis, Properties and Bioimaging Applications of Silver-Based Quantum Dots

2021 ◽  
Vol 22 (22) ◽  
pp. 12202
Author(s):  
Mariya Borovaya ◽  
Inna Horiunova ◽  
Svitlana Plokhovska ◽  
Nadia Pushkarova ◽  
Yaroslav Blume ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Near Infrared ◽  
Semiconductor Nanocrystals ◽  
Current Status ◽  
Synthesis Properties ◽  
Semiconductor Nanomaterials ◽  
Electrooptical Properties ◽  
Basic Features

Ag-based quantum dots (QDs) are semiconductor nanomaterials with exclusive electrooptical properties ideally adaptable for various biotechnological, chemical, and medical applications. Silver-based semiconductor nanocrystals have developed rapidly over the past decades. They have become a promising luminescent functional material for in vivo and in vitro fluorescent studies due to their ability to emit at the near-infrared (NIR) wavelength. In this review, we discuss the basic features of Ag-based QDs, the current status of classic (chemical) and novel methods (“green” synthesis) used to produce these QDs. Additionally, the advantages of using such organisms as bacteria, actinomycetes, fungi, algae, and plants for silver-based QDs biosynthesis have been discussed. The application of silver-based QDs as fluorophores for bioimaging application due to their fluorescence intensity, high quantum yield, fluorescent stability, and resistance to photobleaching has also been reviewed.

Quantum Dots CdSe/ZnS-Loaded Poly(D,L-Lactide-Co-Glycolide) Nanoparticles: Physicochemical Characterization and Application

2006 ◽  
Vol 505-507 ◽  
pp. 667-672 ◽  
Author(s):  
Chih Hui Yang ◽  
Kuo Chin Lin ◽  
Yu Huai Chang ◽  
Yu Cheng Lin
Keyword(s):  
Quantum Dots ◽  
Fluorescence Intensity ◽  
Fluorescence Spectra ◽  
Uv Light ◽  
Physicochemical Characterization ◽  
Plga Nanoparticles ◽  
Optimum Concentration ◽  
Cellular Internalization ◽  
Optimum Concentration Range

This paper described and characterized the quantum dots (QDs) with/without the polymeric PLGA applied in MC3T3E-1 delivery. Neat QDs were treated with various solvents, temperatures, exposure time and concentration to evaluate their stability and efficacy. We found that the intensity degree of fluorescence spectra (QDs) in different solvents follows the order: ether > THF > acetone > chloroform > methanol. Importantly, the QDs become inactive after 8-hr dissolution in the solvents of ether, THF or chloroform. According to this result, acetone and methanol are ideal solvents for QDs. The optimum concentration range of QDs in acetone is 5 to 10 mg/mL. We found that no obvious difference of fluorescence intensity was detected in QDs stored respectively at 4 °C, 24 °C and 44 °C (8-hour). When QDs were exposed to UV light (312 nm) for 2 hr, serious decay of fluorescence intensity was observed. In order to extend the application of QDs in medical areas, we encapsulated them in individual biocompatible poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles for in-vitro imaging of endocytosis in MC3T3E-1 cells. We demonstrated that the polymeric PLGA have the ability to permeate the cells for cellular internalization; the endocytotic activity could be enhanced by the polymeric QDs-encapsulated PLGA.

Nanoparticle-functionalized microcapsules for in vitro delivery and sensing

Nanophotonics ◽  
2012 ◽  
Vol 1 (2) ◽  
pp. 171-180 ◽  
Author(s):  
Susana Carregal-Romero ◽  
Markus Ochs ◽  
Wolfgang J. Parak
Keyword(s):  
Quantum Dots ◽  
Magnetic Nanoparticles ◽  
Optical Excitation ◽  
Building Blocks ◽  
Inorganic Nanoparticles ◽  
Hybrid Particles ◽  
Practical Applications ◽  
Field Gradients ◽  
Magnetic Field Gradients

AbstractInorganic nanoparticles such as magnetic nanoparticles, fluorescent quantum dots, and plasmonic nanoparticles can be used as building blocks for designing multifunctional systems based on polymeric capsules. The properties of the inorganic nanoparticles hereby are harnessed to provide additional functionality to the polymer capsules. Biological applications towards in vitro sensing and delivery are discussed. Examples will be given in which magnetic nanoparticles are used to direct capsules with magnetic field gradients, colloidal quantum dots are used to identify capsules via the formation of optical barcodes, and gold nanoparticles are used as light-controlled heat-sources for opening capsules and releasing macromolecules from their cavity upon optical excitation. This demonstrates that combination of inorganic nanoparticles and organic/polymeric molecules as carrier matrices allow for tailoring multifunctional hybrid particles for practical applications.

Visible-light excited polar Dion-Jacobson Rb(Bi1-xEux)2Ti2NbO10 perovskite: Photoluminescence properties and in-vitro bioimaging

2022 ◽  
Author(s):  
Debendra Prasad Panda ◽  
Akash Kumar Singh ◽  
Tapas Kumar Kundu ◽  
Sundaresan Athinarayanan
Keyword(s):  
Quantum Dots ◽  
Rare Earth ◽  
Visible Light ◽  
Organic Dyes ◽  
Photoluminescence Properties ◽  
Rare Earth Ion

Rare-earth ion-activated oxide phosphors are beneficial to overcome the problems like photobleaching, reduced lifetime, and the blinking of organic dyes and quantum dots for bioimaging applications. In this work, we...

Sign in / Sign up

Export Citation Format

Share Document