Determination of Protein Content in Alhydrogel®-Based Vaccines by O-Phthalaldehyde Assay

2016 ◽  
pp. 263-271
Author(s):  
Kelly M. Rausch ◽  
Daming Zhu
Keyword(s):  
Protein Content

Determination of the protein content in brine from salted herring using near-infrared spectroscopy

LWT ◽  
2004 ◽  
Vol 37 (7) ◽  
pp. 803-809 ◽  
Author(s):  
Vibeke T Svensson ◽  
Henrik Hauch Nielsen ◽  
Rasmus Bro
Keyword(s):  
Infrared Spectroscopy ◽  
Protein Content ◽  
Near Infrared Spectroscopy ◽  
Near Infrared

scholarly journals Determination Of Quality Of Milk From Different Genotype Of Dairy Cows

1970 ◽  
Vol 37 (1) ◽  
pp. 52-56 ◽  
Author(s):  
MR Islam ◽  
MN Hassan ◽  
MSR Siddiki ◽  
MAS Khan ◽  
MA Islam
Keyword(s):  
Specific Gravity ◽  
Dairy Cows ◽  
Protein Content ◽  
Milk Sample ◽  
The Other ◽  
Test Protein ◽  
Quality Of Milk ◽  
Genotype A

The present study was conducted to investigate the milk quality from different genotype. A total 48 dairy cows belong to different genotypes such as Holstein cross, Jersey cross, Sahiwal cross and Red Chittagong were selected. Milk compositional viz. specific gravity, Acidity test, Fat test, Protein test, Solids-not-fat (SNF) test and total solids (TS) test were carried out. It was observed that the higher specific gravity of milk was obtained from Jersey cross and Red Chittagong genotype, which was significantly higher (P<0.01) than the other genotype of dairy cows. The highest acidity (%) was observed (0.165±0.014) in Red Chittagong and the lowest acidity (0.118±0.025) in Sahiwal cross. The highest fat (%) was observed from Red Chittagong cows milk which was statistically higher from Jersey cross and Sahiwal cross and the lowest fat (%) was found in the milk of Holstein cross cows. The highest SNF value was obtained from Jersey cross and the lowest from Sahiwal cross. On the other hand, Holstein cross and Red Chittagong was nearly similar. Red Chittagong milk sample showed the highest TS value and the lowest value was observed from Holstein cross. The highest protein value was found from Red Chittagong and the lowest from Holstein cross but the protein content of other two genotypes were nearly similar. DOI: http://dx.doi.org/10.3329/bjas.v37i1.9868 BJAS 2008; 37(1): 53-56

scholarly journals Condutividade elétrica como indicador de danos por temperaturas baixas em folhas de feijão

2019 ◽  
Vol 40 (3) ◽  
pp. 1011
Author(s):  
Juliandra Rodrigues Rosisca ◽  
Carolina Maria Gaspar de Oliveira ◽  
Altamara Viviane de Souza Sartori ◽  
Renata Stolf-Moreira ◽  
Marcelo Augusto de Aguiar e Silva ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Cold Stress ◽  
Protein Content ◽  
Pearson Correlation ◽  
Total Protein Content ◽  
Leaf Discs ◽  
Randomized Design ◽  
Bean Plants ◽  
C 30

The electrical conductivity test indirectly evaluates cell membrane disorganization by quantifying the electrolytes released into the water after tissue imbibing. The objective of this work was to evaluate methodological variations in the electrical conductivity test, for it to serve as an indicator of low temperature-induced damages and estimate the cold tolerance of bean plants. Cultivar IPR Uirapuru plants were subjected to minimum temperatures of 4 °C, 2 °C, 0 °C, -1 °C, -2 °C, -3 °C, and -4 °C for 1 h in a growth chamber under controlled conditions. After the treatment period, the response of plants to cold stress was evaluated by determination of the total protein content, and catalase (CAT) and ascorbate peroxidase (APX) enzymatic activities, and evaluation of photosystem II (Fm/Fv) efficiency and leaf anatomy. These results were compared with those obtained in the electrical conductivity test, which was performed in plants under cold stress as well as under a non-stress environment, with 2, 4, 6, and 8 leaf discs immersed in 30 mL of distilled water for 24 h in BOD, at temperatures of 25 °C, 30 °C, and 35°C. Analysis of variance was performed using a completely randomized design, and for electrical conductivity, a number of discs × cold stress temperature combinations were used for each soak temperature. The averages were compared using the Turkey's test at 5% and 10% probability. Pearson correlation coefficient (r) between the conductivity averages and other cold stress evaluation data was also performed. The results showed a marked reduction in the ratio (Fv/Fm) only in the treatments at -3 °C and -4°C, which indicated tissue death. At temperatures below 0°C, there was a collapse of the leaf blade tissues, and it was not possible to differentiate the palisade parenchyma from the spongy parenchyma in the treatments at -2°C, -3°C, and -4°C. There was an increase in the protein content since the temperature -3°C. The enzyme activity of CAT decrease at -4°C whereas that of APX increased. In the electrical conductivity test, there was a significant interaction between soak temperature and the number of discs, and an increase in conductivity of the solution with a decrease in temperature was verified in several treatments, among which, the combination that best correlated with the other tests was 25°C with six leaf discs. It was concluded that the electrical conductivity test presents results similar to those obtained from other physiological, biochemical, and anatomical tests, and therefore, it can be used to evaluate the damage caused by low temperatures in bean plants.

Direct and Indirect Determination of True Protein Content of Milk by Kjeldahl Analysis: Collaborative Study

1991 ◽  
Vol 74 (2) ◽  
pp. 281-288 ◽  
Author(s):  
David M Barbano ◽  
Joanna M Lynch ◽  
J Richard Fleming
Keyword(s):  
Nitrogen Content ◽  
Protein Content ◽  
Total Nitrogen ◽  
Collaborative Study ◽  
Total Nitrogen Content ◽  
Nonprotein Nitrogen ◽  
Protein Nitrogen ◽  
Nitrogen Determination ◽  
True Protein

Abstract Currently, the reference procedure for determination of the "protein" content of milk is based on measurement of the total nitrogen content of milk by the Kjeldahl method (AOAC method, 920.105). About 6% of the total nitrogen content of milk Is nonprotein nitrogen. Therefore, total nitrogen multiplied by the conversion factor 6.38 overestimates the true protein content of milk on average by about 6%. In the present study, new direct and Indirect methods were developed for measurement of the true protein content of whole milk by Kjeldahl nitrogen determination. Both new methods are sample preparation procedures used to fractionate the nitrogen-containing compounds In milk prior to measurement of the nitrogen content of these fractions by Kjeldahl analysis. The collaborative study consisted of 9 pairs of blind duplicate milk samples that were analyzed for total nitrogen, nonprotein nitrogen, and protein nitrogen by each of 10 laboratories. Both methods for true protein measurement (direct and Indirect) gave acceptable statistical performance characteristics and good agreement between methods. The new direct method requires about half the laboratory analysis work of the indirect method (i.e., total minus nonprotein nitrogen). The methods have been adopted official first action by AOAC as (1) a new method for nonprotein nitrogen determination in milk, (2) a new method (direct) for determination of protein nitrogen content of milk, and {3) an alternative method (indirect) for determination of protein nitrogen content of milk.

Retardation Signal for Fluorescent Determination of Total Protein Content via Rapid and Sensitive Chip Moving Reaction Boundary Electrophoretic Titration

2014 ◽  
Vol 86 (6) ◽  
pp. 2888-2894 ◽  
Author(s):  
Houyu Wang ◽  
Yongting Shi ◽  
Jian Yan ◽  
Jingyu Dong ◽  
Si Li ◽  
...  
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Total Protein Content

scholarly journals Mid-Infrared Spectroscopy (MIR) for Simultaneous Determination of Fat and Protein Content in Meat of Several Animal Species

2017 ◽  
Vol 10 (10) ◽  
pp. 3462-3470 ◽  
Author(s):  
M. Lozano ◽  
P. Rodríguez-Ulibarri ◽  
J. C. Echeverría ◽  
M. Beruete ◽  
M. Sorolla ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Protein Content ◽  
Simultaneous Determination ◽  
Animal Species ◽  
Mid Infrared ◽  
Mid Infrared Spectroscopy
Sign in / Sign up

Export Citation Format

Share Document