Automated Live Microscopy to Study Mitotic Gene Function in Fluorescent Reporter Cell Lines

2009 ◽  
pp. 113-134 ◽  
Author(s):  
Michael H.A. Schmitz ◽  
Daniel W. Gerlich
Keyword(s):  
Cell Lines ◽  
Gene Function ◽  
Reporter Cell ◽  
Fluorescent Reporter

Pharmacological characterization of receptor guanylyl cyclase reporter cell lines

2013 ◽  
Vol 698 (1-3) ◽  
pp. 131-136 ◽  
Author(s):  
Frank Wunder ◽  
Annette Woermann ◽  
Andreas Geerts ◽  
Markus Milde
Keyword(s):  
Cell Lines ◽  
Guanylyl Cyclase ◽  
Reporter Cell ◽  
Pharmacological Characterization

scholarly journals Generation of Double-Labeled Reporter Cell Lines for Studying Co-Dynamics of Endogenous Proteins in Individual Human Cells

PLoS ONE ◽  
2010 ◽  
Vol 5 (10) ◽  
pp. e13524 ◽  
Author(s):  
Irina Issaeva ◽  
Ariel A. Cohen ◽  
Eran Eden ◽  
Cellina Cohen-Saidon ◽  
Tamar Danon ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cells ◽  
Reporter Cell ◽  
Endogenous Proteins ◽  
Individual Human

scholarly journals Pharmacological characterization of receptor guanylyl cyclase reporter cell lines

2009 ◽  
Vol 9 (S1) ◽  
Author(s):  
Frank Wunder ◽  
Daniel Barufe ◽  
Annette Woermann ◽  
Markus Milde
Keyword(s):  
Cell Lines ◽  
Guanylyl Cyclase ◽  
Reporter Cell ◽  
Pharmacological Characterization

Reporter cell lines are useful tools for monitoring biological activity of nuclear receptor ligands

Luminescence ◽  
2001 ◽  
Vol 16 (2) ◽  
pp. 153-158 ◽  
Author(s):  
Patrick Balaguer ◽  
Anne-Marie Boussioux ◽  
Ediz Demirpence ◽  
Jean-Claude Nicolas
Keyword(s):  
Biological Activity ◽  
Cell Lines ◽  
Nuclear Receptor ◽  
Receptor Ligands ◽  
Reporter Cell ◽  
Nuclear Receptor Ligands

scholarly journals Transgenic human ES and iPS reporter cell lines for identification and selection of pluripotent stem cells in vitro

2014 ◽  
Vol 13 (2) ◽  
pp. 251-261 ◽  
Author(s):  
Dmitry A. Ovchinnikov ◽  
Drew M. Titmarsh ◽  
Patrick R.J. Fortuna ◽  
Alejandro Hidalgo ◽  
Samah Alharbi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Lines ◽  
Pluripotent Stem Cells ◽  
Reporter Cell ◽  
Selection Of

scholarly journals Generation of a Jurkat-based fluorescent reporter cell line to evaluate lipid antigen interaction with the human iNKT cell receptor

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Piotr Humeniuk ◽  
Sabine Geiselhart ◽  
Claire Battin ◽  
Tonya Webb ◽  
Peter Steinberger ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Receptor ◽  
Inkt Cell ◽  
Reporter Cell Line ◽  
Reporter Cell ◽  
Fluorescent Reporter ◽  
Lipid Antigen ◽  
Antigen Interaction

scholarly journals Development of a Bicistronic Vector for the Expression of a CRISPR/Cas9-mCherry System in Fish Cell Lines

Cells ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 75 ◽  
Author(s):  
Sebastian Escobar-Aguirre ◽  
Duxan Arancibia ◽  
Amanda Escorza ◽  
Cristián Bravo ◽  
María Andrés ◽  
...  
Keyword(s):  
Genome Editing ◽  
Cell Lines ◽  
Gene Function ◽  
Fish Cell ◽  
Fish Cell Lines ◽  
Guide Rna ◽  
Bicistronic Vector ◽  
Expression Platform ◽  
Fish Cells ◽  
U6 Rna

The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system has been widely used in animals as an efficient genome editing tool. In fish cells, the technique has been difficult to implement due to the lack of proper vectors that use active promoters to drive the expression of both small guide RNA (sgRNA) and the S. pyogenes Cas9 (spCas9) protein within a single expression platform. Until now, fish cells have been modified using co-transfection of the mRNA of both the sgRNA and the spCas9. In the present study, we describe the optimization of a new vector for the expression of a CRISPR/Cas9 system, designed to edit the genome of fish cell lines, that combines a gene reporter (mCherry), sgRNA, and spCas9 in a single vector, facilitating the study of the efficiency of piscine and non-piscine promoters. A cassette containing the zebrafish U6 RNA III polymerase (U6ZF) promoter was used for the expression of the sgRNA. The new plasmid displayed the expression of spCas9, mCherry, and sgRNA in CHSE/F fish cells. The results demonstrate the functionality of the mammalian promoter and the U6ZF promoter in fish cell lines. This is the first approach aimed at developing a unified genome editing system in fish cells using bicistronic vectors, thus creating a powerful biotechnological platform to study gene function.

Influence of amide versus ester linkages on the anticancer properties of the new flavone–biotin conjugates

2019 ◽  
Vol 74 (7-8) ◽  
pp. 193-200
Author(s):  
Monika Stompor ◽  
Marta Świtalska ◽  
Agata Bajek ◽  
Joanna Wietrzyk
Keyword(s):  
Cell Lines ◽  
Amide Bond ◽  
Step Method ◽  
Fluorescent Reporter ◽  
Bifunctional Molecules ◽  
Anticancer Properties ◽  
One Step ◽  
3T3 Cell Lines ◽  
Mcf 7

Abstract Novel biotinylated C-6 substituted flavones were synthesised by a one-step method that connects biotin to 6-hydroxyflavone and 6-aminoflavone by esterification and amidation of hydroxyl and amino groups, respectively. The obtained compounds, 6-O-biotinylflavone and 6-biotinylamidoflavone, are the bifunctional molecules composed of a flavone moiety as a fluorescent reporter and biotin as a cancer-targeting unit. Antiproliferative activity was evaluated using SRB assays in MCF-7, MCF-10A, HepG2, MDA-MB-231, 4T1, and Balb/3T3 cell lines. In vitro evaluation revealed that compounds with biotin moiety displayed better cell selectivity between the cancer and normal cells than the parental substrates. These results indicate that anticancer effect is not related to the position of biotin moiety, but it is related to the presence of ester or amide bond. 6-O-Biotinylflavone was more active than 6-hydroxyflavone against human breast (MDA-MB-231) and liver (HepG2) cancer cells with IC50 (concentration of tested agent that inhibits proliferation of the cell population by 50%) values equal to 78.5 ± 18.8 μM and 133.2 ± 14.2 μM, respectively. Non biotinylated 6-aminoflavone was more active than 6-biotinylamidoflavone against all tested cell lines, with IC50 values between 34.3 ± 9.1 μM (4T1) and 173.86 ± 24.3 μM (MCF-7).

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