Two-Dimensional vs. Three-Dimensional In Vitro Tumor Migration and Invasion Assays

2013 ◽  
pp. 227-252 ◽  
Author(s):  
Miriam Zimmermann ◽  
Carol Box ◽  
Suzanne A. Eccles
Keyword(s):  
Three Dimensional ◽  
Migration And Invasion ◽  
Two Dimensional ◽  
Invasion Assays

Establishment of Two Dimensional (2D) and Three-Dimensional (3D) Melanoma Primary Cultures as a Tool for In Vitro Drug Resistance Studies

2019 ◽  
pp. 119-131 ◽  
Author(s):  
Nataly Cruz Rodríguez ◽  
Johanna Lineros ◽  
Carol Stefany Rodríguez ◽  
Lina María Martínez ◽  
Josefa Antonia Rodríguez
Keyword(s):  
Drug Resistance ◽  
Primary Cultures ◽  
Three Dimensional ◽  
Two Dimensional

scholarly journals Recapitulating tumour microenvironment in chitosan–gelatin three-dimensional scaffolds: an improved in vitro tumour model

2012 ◽  
Vol 9 (77) ◽  
pp. 3288-3302 ◽  
Author(s):  
Neha Arya ◽  
Viren Sardana ◽  
Meera Saxena ◽  
Annapoorni Rangarajan ◽  
Dhirendra S. Katti
Keyword(s):  
Cancer Progression ◽  
Expression Profiles ◽  
Three Dimensional ◽  
Gene Expression Profiles ◽  
Petri Dish ◽  
Tumour Microenvironment ◽  
Two Dimensional ◽  
Tumour Model

Owing to the reduced co-relationship between conventional flat Petri dish culture (two-dimensional) and the tumour microenvironment, there has been a shift towards three-dimensional culture systems that show an improved analogy to the same. In this work, an extracellular matrix (ECM)-mimicking three-dimensional scaffold based on chitosan and gelatin was fabricated and explored for its potential as a tumour model for lung cancer. It was demonstrated that the chitosan–gelatin (CG) scaffolds supported the formation of tumoroids that were similar to tumours grown in vivo for factors involved in tumour-cell–ECM interaction, invasion and metastasis, and response to anti-cancer drugs. On the other hand, the two-dimensional Petri dish surfaces did not demonstrate gene-expression profiles similar to tumours grown in vivo . Further, the three-dimensional CG scaffolds supported the formation of tumoroids, using other types of cancer cells such as breast, cervix and bone, indicating a possible wider potential for in vitro tumoroid generation. Overall, the results demonstrated that CG scaffolds can be an improved in vitro tool to study cancer progression and drug screening for solid tumours.

Associated Anisotropy of intrinsic NAD(P)H for monitoring changes in the metabolic activities of breast cancer cells in three-dimensional collagen matrix

2021 ◽  
Author(s):  
Anh Cong ◽  
Rafaela M. L. Pimenta ◽  
Jon Holy ◽  
Ahmed A Heikal
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Breast Cancer Cells ◽  
Monolayer Culture ◽  
Three Dimensional ◽  
Collagen Matrix ◽  
Living Cells ◽  
Two Dimensional ◽  
Metabolic Activities

The majority of in vitro studies of living cells are routinely conducted in a two-dimensional (2D) monolayer culture. Recent studies, however, suggest that 2D cell culture promotes specific types of...

Micro-CT analysis of two-dimensional and three-dimensional parameters in severely curved roots prepared with 3 instrumentation systems: An in vitro study

2020 ◽  
Vol 9 (2) ◽  
pp. 62
Author(s):  
AntonioMiranda da Cruz-Filho ◽  
LuisEduardo Souza-Flamini ◽  
BrunoMonguilhott Crozeta ◽  
RicardoGariba Silva ◽  
RicardoNovak Savioli ◽  
...  
Keyword(s):  
Three Dimensional ◽  
In Vitro Study ◽  
Vitro Study ◽  
Two Dimensional ◽  
Micro Ct ◽  
Ct Analysis ◽  
Dimensional Parameters

Three-Dimensional Volumetric Ultrasound Imaging of Arterial Pathology from Two-Dimensional Intravascular Ultrasound: An in Vitro Study

Angiology ◽  
1994 ◽  
Vol 45 (4) ◽  
pp. 253-264 ◽  
Author(s):  
Krishnaswamy Chandrasekaran ◽  
Chandra M. Sehgal ◽  
Tsui-Lieh Hsu ◽  
Nancy A. Young ◽  
Arthur J. D'Adamo ◽  
...  
Keyword(s):  
Intravascular Ultrasound ◽  
Ultrasound Imaging ◽  
Three Dimensional ◽  
In Vitro Study ◽  
Vitro Study ◽  
Two Dimensional ◽  
Volumetric Ultrasound

scholarly journals A three-dimensional model of the human blood-brain barrier to analyse the transport of nanoparticles and astrocyte/endothelial interactions

F1000Research ◽  
2016 ◽  
Vol 4 ◽  
pp. 1279 ◽  
Author(s):  
Peddagangannagari Sreekanthreddy ◽  
Radka Gromnicova ◽  
Heather Davies ◽  
James Phillips ◽  
Ignacio A. Romero ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Human Blood ◽  
Three Dimensional ◽  
Collagen Gel ◽  
Cell Types ◽  
Brain Barrier ◽  
Two Dimensional ◽  
Three Dimensional Model ◽  
Blood Brain

The aim of this study was to develop a three-dimensional (3D) model of the human blood-brain barrier in vitro, which mimics the cellular architecture of the CNS and could be used to analyse the delivery of nanoparticles to cells of the CNS. The model includes human astrocytes set in a collagen gel, which is overlaid by a monolayer of human brain endothelium (hCMEC/D3 cell line). The model was characterised by transmission electron microscopy (TEM), immunofluorescence microscopy and flow cytometry. A collagenase digestion method could recover the two cell types separately at 92-96% purity.  Astrocytes grown in the gel matrix do not divide and they have reduced expression of aquaporin-4 and the endothelin receptor, type B compared to two-dimensional cultures, but maintain their expression of glial fibrillary acidic protein. The effects of conditioned media from these astrocytes on the barrier phenotype of the endothelium was compared with media from astrocytes grown conventionally on a two-dimensional (2D) substratum. Both induce the expression of tight junction proteins zonula occludens-1 and claudin-5 in hCMEC/D3 cells, but there was no difference between the induced expression levels by the two media. The model has been used to assess the transport of glucose-coated 4nm gold nanoparticles and for leukocyte migration. TEM was used to trace and quantitate the movement of the nanoparticles across the endothelium and into the astrocytes. This blood-brain barrier model is very suitable for assessing delivery of nanoparticles and larger biomolecules to cells of the CNS, following transport across the endothelium.

scholarly journals Eribulin activity in soft tissue sarcoma monolayer and three-dimensional cell line models: could the combination with other drugs improve its antitumoral effect?

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Javier Escudero ◽  
Victoria Heredia-Soto ◽  
Yinyin Wang ◽  
Patricia Ruiz ◽  
Yingying Hu ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Cell Line ◽  
Antitumour Activity ◽  
Three Dimensional ◽  
Soft Tissue Sarcomas ◽  
Migration And Invasion ◽  
2D And 3D ◽  
First Time ◽  
Dimensional Cell

Abstract Background Eribulin has shown antitumour activity in some soft tissue sarcomas (STSs), but it has only been approved for advanced liposarcoma (LPS). Methods In this study, we evaluated the effect of eribulin on proliferation, migration and invasion capabilities in LPS, leiomyosarcoma (LMS) and fibrosarcoma (FS) models, using both monolayer (2D) and three-dimensional (3D) spheroid cell cultures. Additionally, we explored combinations of eribulin with other drugs commonly used in the treatment of STS with the aim of increasing its antitumour activity. Results Eribulin showed activity inhibiting proliferation, 2D and 3D migration and invasion in most of the cell line models. Furthermore, we provide data that suggest, for the first time, a synergistic effect with ifosfamide in all models, and with pazopanib in LMS as well as in myxoid and pleomorphic LPS. Conclusions Our results support the effect of eribulin on LPS, LMS and FS cell line models. The combination of eribulin with ifosfamide or pazopanib has shown in vitro synergy, which warrants further clinical research.

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