MRI of Early OA | ScienceGate

Synovitis of the knee synovium is proven to be a precursor of knee osteoarthritis (OA), leading to a radiologically advanced stage of the disease. This study was conducted to elucidate the expression pattern of different inflammatory factors—NF-kB, iNOS, and MMP-9 in a subpopulation of synovial cells. Thirty synovial membrane intra-operative biopsies of patients (ten controls, ten with early OA, and ten with advanced OA, according to the Kellgren–Lawrence radiological score) were immunohistochemically stained for NF-kB, iNOS, and MMP9, and for different cell markers for macrophages, fibroblasts, leukocytes, lymphocytes, blood vessel endothelial cells, and blood vessel smooth muscle cells. The total number of CD68+/NF-kB+ cells/mm2 in the intima of early OA patients (median = 2359) was significantly higher compared to the total number of vimentin+/Nf-kB+ cells/mm2 (median = 1321) and LCA+/NF-kB+ cells/mm2 (median = 64) (p < 0.001 and p < 0.0001, respectively). The total number of LCA+/NF-kB+ cells/mm2 in the subintima of advanced OA patients (median = 2123) was significantly higher compared to the total number of vimentin+/NF-kB+ cells/mm2 (median = 14) and CD68+/NF-kB+ cells/mm2 (median = 29) (p < 0.0001). The total number of CD68+/iNOS+ cells/mm2 in the intima of both early and advanced OA patients was significantly higher compared to the total number of vimentin+/iNOS+ cells/mm2 and LCA+/iNOS+ cells/mm2 (p < 0.0001 and p < 0.001, respectively). The total number of CD68+/MMP-9+ cells/mm2 in the intima of both early and advanced OA patients was significantly higher compared to the total number of vimentin+/MMP-9+ cells/mm2 and CD5+/MMP-9+ cells/mm2 (p < 0.0001). Macrophages may have a leading role in OA progression through the NF-kB production of inflammatory factors (iNOS and MMP-9) in the intima, except in advanced OA, where leukocytes could have a dominant role through NF-kB production in subintima. The blocking of macrophageal and leukocyte NF-kB expression is a possible therapeutic target as a disease modifying drug.

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Platelet-rich plasma as an effective biological therapy in early-stage knee osteoarthritis: One year follow up

SICOT-J ◽

10.1051/sicotj/2021003 ◽

2021 ◽

Vol 7 ◽

pp. 6

Author(s):

Deepak Rai ◽

Jyotsana Singh ◽

Thimmappa Somashekharappa ◽

Ajit Singh

Keyword(s):

Knee Osteoarthritis ◽

Early Stage ◽

Platelet Rich Plasma ◽

Symptomatic Relief ◽

Womac Questionnaire ◽

Significant Clinical Improvement ◽

Bioactive Agents ◽

One Year ◽

Early Oa

Objective: PRP is produced by centrifugation of whole blood containing highly concentrated platelets, associated growth factors, and other bioactive agents which has been shown to provide some symptomatic relief in early knee osteoarthritis (OA). The principal objective of our study was to evaluate the effectiveness and safety of standardized intra-articular injection of autologous PRP in early osteoarthritis knee. Methods: A total of 98 eligible symptomatic patients received two injections of standardized PRP 3 weeks apart. Clinical outcomes were evaluated using the VAS and Western Ontario and McMaster Universities Arthritis Index (WOMAC) questionnaire before treatment and at 6 weeks, 3 months, 6 months, and 1 year after treatment. Secondary objectives were safety (side effects), and the effect of PRP on the different grades of knee degeneration. Results: There was a statistically significant improvement in mean VAS and WOMAC scores at 6 weeks, 3 months, 6 months, and slight loss of improvement at 1 year follow-up. There was also a correlation between the degree of degeneration and improvement in the mean scores. The decrease in mean pain score is more in grades 1 and 2 (early OA) than in grade 3. The intraarticular injection is safe, with no major complications. Conclusion: PRP is a safe and effective biological regenerative therapy for early OA Knees. It provides a significant clinical improvement in patients with some loss of improvement with time. More studies will be needed to confirm our findings.

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L-PRF in Osteoarthritis Treatment: Results of a Pilot study

Journal of Regenerative Biology and Medicine ◽

10.37191/mapsci-2582-385x-2(6)-048 ◽

2020 ◽

Author(s):

Valerio Di Nicola

Keyword(s):

Primary Outcome ◽

Musculoskeletal Disorder ◽

Chronic Wound ◽

Bone Erosion ◽

Treatment Results ◽

Platelet Rich Fibrin ◽

Knee Oa ◽

Osteoarthritis Treatment ◽

Connective Tissue Regeneration ◽

Early Oa

Osteoarthritis (OA) is the most common chronic musculoskeletal disorder and is the most frequent single cause of disability in older adults [1]. OA is a chronic disease progressively involving the entire joint. Progression involves capsule-bursa inflammation, synovial fluid modifications, cartilage erosions, and osteochondral inflammatory deteriorations leading to bone erosion and distortion [1]. Early OA defines the initial cascade of events that trigger the disease and lead to the full-blown OA. The disease progression can sometimes last for years being quite often neglected or mistreated with palliative medications. Joint resident MSCs has always been a target for our research into and treatment of OA [1,2,3]. Recently L-PRF (leukocyte and platelet-rich fibrin), showed promising properties in connective tissue regeneration and, for this reason, is now widely applied in chronic wound healing and jawbone growth [4,5,6]. After centrifugation, L-PRF membranes hold vital platelets, leukocytes, and various peripheral blood cells [7,8]. As a result, we exposed these membranes to a thermic shock aimed to increase the pool of HSPs. The final product was named supercharged L-PRF. Supercharged L-PRF components -membranes and hyper-acute serum- were used in knee OA patients in a small preliminary comparative study. 20 consecutive patients were randomly divided into 2 groups: 10 patients treated with supercharged L-PRF and 10 with PRP+HA (PRP+ Hyaluronic acid). The primary outcome of this study was to induce persistent pain relief and recovery of motility. This article reports supercharged L-PRF preliminary experience in degenerative OA treatment.

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Expression of Long-Chain Noncoding RNA GAS5 in Osteoarthritis And Its Effect on Apoptosis And Autophagy of Osteoarthritis Chondrocytes

10.21203/rs.3.rs-48469/v1 ◽

2020 ◽

Author(s):

Qinghui Ji ◽

Xiaofeng Qiao ◽

Yongxiang Liu ◽

Dawei Wang ◽

Jinglong Yan

Keyword(s):

Articular Cartilage ◽

Noncoding Rna ◽

Target Gene ◽

Mtor Inhibitor ◽

Cartilage Tissue ◽

Non Coding Rna ◽

Osteoarthritis Chondrocytes ◽

Oa Chondrocytes ◽

Early Oa ◽

Long Chain

Abstract Objective: To investigate the expression of long chain non-coding RNA GAS5 in osteoarthritis(OA) and the effect on autophagy and apoptosis of chondrocytes. Method: OA rat model was constructed and the expressions of GAS5 in articular cartilage tissues at 4 weeks(early OA) and 12 weeks(late OA) after modeling were detected. The chondrocytes of OA rat were isolated, cultured and transfected with si-GAS5 to silence the GAS5. Then the change of apoptosis and autophagy levels of OA chondrocytes were detected. The microRNA binding to GAS5 and the competitive target gene were analyzed and verified. Results: The expression of GAS5 in cartilage tissue of OA rats was higher than that of control, which was higher in late OA than that in early OA. After silencing the GAS5, the autophagy ability of OA chondrocytes was increased and the apoptosis rate was decreased. GAS5 could bind with miR-144 competitively and regulate mTOR positively. The mTOR inhibitor GDC-0349 could reverse the inhibition of GAS5 on cell autophagy but could not reverse the promotion on apoptosis. Conclusion: GAS5 expression was high and increased with the progression of OA. GAS5 could inhibit the autophagy and promote the apoptosis of OA chondrocytes, and the effect on autophagy may be related to the regulation on mTOR.

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Arthroscopic Treatment in Early OA of the Knee

The Journal of the Korean Orthopaedic Association ◽

10.4055/jkoa.2004.39.1.102 ◽

2004 ◽

Vol 39 (1) ◽

pp. 102

Author(s):

Sung Jea Kim

Keyword(s):

Arthroscopic Treatment ◽

Early Oa

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Quantitative Radiographic Features of Early Knee Osteoarthritis: Development Over 5 Years and Relationship with Symptoms in the CHECK Cohort

The Journal of Rheumatology ◽

10.3899/jrheum.120320 ◽

2012 ◽

Vol 40 (1) ◽

pp. 58-65 ◽

Cited By ~ 23

Author(s):

MARGOT B. KINDS ◽

ANNE C.A. MARIJNISSEN ◽

JOHANNES W.J. BIJLSMA ◽

MAARTEN BOERS ◽

FLORIS P.J.G. LAFEBER ◽

...

Keyword(s):

Bone Density ◽

Clinical Outcome ◽

Joint Space Width ◽

Joint Damage ◽

Computer Assisted ◽

Digital Analysis ◽

Radiographic Features ◽

Early Oa ◽

Varus Angle ◽

Over Time

Objective.To evaluate whether computer-assisted, interactive digital analysis of knee radiographs enables identification of different quantitative features of joint damage, and to evaluate the relationship of such features with each other and with clinical characteristics during 5-year followup in early osteoarthritis (OA).Methods.Knee radiographs from the Cohort Hip and Cohort Knee (CHECK) study, including 1002 individuals with early OA complaints, were evaluated for different measures with knee images digital analysis (KIDA). To aid definition of different radiographic features of OA, principal component analysis of KIDA was used. Features were correlated (Pearson) to each other, evaluated for changes over time, and related to clinical outcome (Western Ontario and McMaster Universities Osteoarthritis Index for pain and function) using baseline, 2-year, and 5-year followup data.Results.The identified radiographic features were joint space width (JSW: minimum, medial, lateral), varus angle, osteophyte area, eminence height, and bone density. The features progressed in severity at different times during followup: early (medial JSW, osteophyte area), late (minimum and lateral JSW, eminence height), and both early and late (varus angle, bone density). Correlations between different radiographic features varied between timepoints. The JSW features were most strongly related to each other (largest r = 0.82), but also, e.g., osteophytes and bone density were correlated (largest r = 0.33). The relationships with clinical outcome varied over time, but were most commonly found for osteophyte area and JSW.Conclusion.In this early OA cohort, different radiographic features were identified that progressed at different rates between timepoints. The relations between radiographic features and with clinical outcome varied over time. This implies that longitudinal evaluation of different features can improve insight into progression of OA.

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