Involvement of Follicular Basement Membrane and Vascular Endothelium in Blood-Follicle Barrier Formation of Mice

2016 ◽  
pp. 149-153 ◽  
Author(s):  
Nobuhiko Ohno ◽  
Hong Zhou ◽  
Nobuo Terada ◽  
Shinichi Ohno
Keyword(s):  
Basement Membrane ◽  
Vascular Endothelium ◽  
Barrier Formation

scholarly journals ApoE (Apolipoprotein E) in Brain Pericytes Regulates Endothelial Function in an Isoform-Dependent Manner by Modulating Basement Membrane Components

2020 ◽  
Vol 40 (1) ◽  
pp. 128-144 ◽  
Author(s):  
Yu Yamazaki ◽  
Mitsuru Shinohara ◽  
Akari Yamazaki ◽  
Yingxue Ren ◽  
Yan W. Asmann ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Basement Membrane ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Cell Phenotype ◽  
Dependent Manner ◽  
Barrier Formation ◽  
Brain Pericytes ◽  
Membrane Components ◽  
And Function

Objective: The ε4 allele of the APOE gene ( APOE4 ) is the strongest genetic risk factor for Alzheimer disease when compared with the common ε3 allele. Although there has been significant progress in understanding how apoE4 (apolipoprotein E4) drives amyloid pathology, its effects on amyloid-independent pathways, in particular cerebrovascular integrity and function, are less clear. Approach and Results: Here, we show that brain pericytes, the mural cells of the capillary walls, differentially modulate endothelial cell phenotype in an apoE isoform-dependent manner. Extracellular matrix protein induction, tube-like structure formation, and barrier formation were lower with endothelial cells cocultured with pericytes isolated from apoE4-targeted replacement (TR) mice compared with those from apoE3-TR mice. Importantly, aged apoE4-targeted replacement mice had decreased extracellular matrix protein expression and increased plasma protein leakages compared with apoE3-TR mice. Conclusions: ApoE4 impairs pericyte-mediated basement membrane formation, potentially contributing to the cerebrovascular effects of apoE4.

scholarly journals Involvement of follicular basement membrane and vascular endothelium in blood–follicle barrier formation of mice revealed by ‘in vivo cryotechnique’

Reproduction ◽  
2007 ◽  
Vol 134 (2) ◽  
pp. 307-317 ◽  
Author(s):  
Hong Zhou ◽  
Nobuhiko Ohno ◽  
Nobuo Terada ◽  
Sei Saitoh ◽  
Yasuhisa Fujii ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Blood Vessels ◽  
Vascular Endothelium ◽  
Serum Proteins ◽  
Vascular Endothelial Cells ◽  
Follicular Development ◽  
Basement Membranes ◽  
In Vivo Cryotechnique ◽  
Quick Freezing

The molecular sieve with size- and charge selectivity in ovarian follicles, the so-called blood–follicle barrier (BFB), was examined during follicular development under physiological conditions to reveal ovarian structures responsible for the BFB by using our ‘in vivocryotechnique’ (IVCT). Mouse ovary specimens were prepared with different methods including IVCT, immersion, or perfusion chemical fixation and quick-freezing following resection or perfusion. Their paraffin sections or cryosections were stained with hematoxylin–eosin or immunostained for serum proteins with different molecular weights: albumin, immunoglobulin (Ig) G1 heavy chain, inter-α-trypsin inhibitor (IαI), fibrinogen, and IgM. Their immunoreactivity was better preserved with IVCT. The immunostaining for albumin was clearly observed in blood vessels, interstitium, and developing follicles, but that of IgG1, IαI, or fibrinogen was significantly decreased inside the follicles. IgM was immunohistochemically decreased throughout the interstitium outside blood vessels. The immunoreactivities of IgG1 and IgM, as compared with albumin, were clearly changed along follicular basement membranes and around vascular endothelial cells respectively. These findings indicate that BFB functions throughout follicular development, and the follicular basement membrane and the vascular endothelium could play some significant roles in the permselectivity for such soluble proteins with intermediate and high molecular weight respectively.

Evidence of Placental Microangiopathy in Pre-Eclamptic States

1975 ◽  
Author(s):  
D. Chervet ◽  
F. Beguin ◽  
P. Vassiliakos ◽  
J. Cox ◽  
C. A. Bouvier
Keyword(s):  
Basement Membrane ◽  
Vascular Endothelium ◽  
Renal Involvement ◽  
Maternal Blood ◽  
Biological Data ◽  
Consumption Coagulopathy ◽  
Present Evidence ◽  
Vascular Basement Membrane ◽  
Before And After ◽  
Fibrin Thrombi

It has been suggested that a consumption coagulopathy does occur in pre-eclampsia and this has been related to intravascular clotting at the glomerular level and in the decidual spiroid arteries of the mother, but surprisingly little studies of the placental vessels have been reported. From biological data before and after delivery, and conventional, electronic and immunofluorescent studies of the placenta at birth, we present evidence for a consumption coagulopathy, renal involvement and diminution of placental secretion before delivery, and demonstrate the anatomopathologrcal substrate for microangiopathy at the placental level. The predominant feature is intense thickening of both trophoblastic and vascular basement membrane in the villosities, intravascular clotting with platelets and fibrin thrombi, and striking modifications of vascular endothelium. While numerous trophoblastic syncytial cells have been found in circulating maternal blood, little or no circulating endothelium has been found so far, probably because they do not cross the thickened trophoblastic basement membrane. It is interesting to consider the described lesions in comparison with those observed in graft-rejection.

Endotoxin Alteration of the Mucopolysaccharide Blood Vessel Coating in Rats

1974 ◽  
Vol 32 ◽  
pp. 148-149
Author(s):  
D. E. Philpott ◽  
A. Takahashi
Keyword(s):  
Skeletal Muscle ◽  
Endothelial Cells ◽  
Salivary Gland ◽  
Carotid Artery ◽  
Basement Membrane ◽  
Coronary Vessels ◽  
Ruthenium Red ◽  
E Coli ◽  
Old Rats ◽  
The Brain

Two month, eight month and two year old rats were treated with 10 or 20 mg/kg of E. Coli endotoxin I. P. The eight month old rats proved most resistant to the endotoxin. During fixation the aorta, carotid artery, basil arartery of the brain, coronary vessels of the heart, inner surfaces of the heart chambers, heart and skeletal muscle, lung, liver, kidney, spleen, brain, retina, trachae, intestine, salivary gland, adrenal gland and gingiva were treated with ruthenium red or alcian blue to preserve the mucopolysaccharide (MPS) coating. Five, 8 and 24 hrs of endotoxin treatment produced increasingly marked capillary damage, disappearance of the MPS coating, edema, destruction of endothelial cells and damage to the basement membrane in the liver, kidney and lung.

Physical Properties of Isolated Perfused Renal Tubular Basement Membranes

1971 ◽  
Vol 29 ◽  
pp. 390-391
Author(s):  
Jared Grantham ◽  
Larry Welling
Keyword(s):  
Basement Membrane ◽  
Basement Membranes ◽  
Transport Mechanisms ◽  
Permeability Characteristics ◽  
Peritubular Capillaries ◽  
Strategic Location ◽  
Tubular Lumen ◽  
Glomerular Filtrate ◽  
Urine Formation ◽  
Renal Tubular

In the course of urine formation in mammalian kidneys over 90% of the glomerular filtrate moves from the tubular lumen into the peritubular capillaries by both active and passive transport mechanisms. In all of the morphologically distinct segments of the renal tubule, e.g. proximal tubule, loop of Henle and distal nephron, the tubular absorbate passes through a basement membrane which rests against the basilar surface of the epithelial cells. The basement membrane is in a strategic location to affect the geometry of the tubules and to influence the movement of tubular absorbate into the renal interstitium. In the present studies we have determined directly some of the mechanical and permeability characteristics of tubular basement membranes.

Kalinin: A new epithelial basement membrane adhesion molecule

1991 ◽  
Vol 49 ◽  
pp. 178-179
Author(s):  
Douglas R. Keene ◽  
Gregory P. Lunstrum ◽  
Patricia Rousselle ◽  
Robert E. Burgeson
Keyword(s):  
Basement Membrane ◽  
Culture Media ◽  
Polyacrylamide Gel Electrophoresis ◽  
Human Keratinocytes ◽  
Positive Staining ◽  
Human Amnion ◽  
Epithelial Basement Membrane ◽  
Type Vii Collagen ◽  
Keratinocyte Cell ◽  
Epithelial Basement

A mouse monoclonal antibody produced from collagenase digests of human amnion was used by LM and TEM to study the distribution and ultrastructural features of an antigen present in epithelial tissues and in cultured human keratinocytes, and by immunoaffinity chromatography to partially purify the antigen from keratinocyte cell culture media.By immunofluorescence microscopy, the antigen displays a tissue distribution similar to type VII collagen; positive staining of the epithelial basement membrane is seen in skin, oral mucosa, trachea, esophagus, cornea, amnion and lung. Images from rotary shadowed preparations isolated by affinity chromatography demonstrate a population of rod-like molecules 107 nm in length, having pronounced globular domains at each end. Polyacrylamide gel electrophoresis suggests that the size of this molecule is approximately 440kDa, and that it is composed of three nonidentical chains disulfide bonded together.

Scanning Electron Microscopy of Human Jejunum in Whipple's Disease

1977 ◽  
Vol 35 ◽  
pp. 354-355
Author(s):  
John H. L. Watson ◽  
C. N. Sun
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Basement Membrane ◽  
Lamina Propria ◽  
Brush Border ◽  
Whipple’S Disease ◽  
Whipple's Disease ◽  
Biopsy Specimens ◽  
Brush Borders ◽  
Scanning Electron

That the etiology of Whipple's disease could be bacterial was first suggested from electron micrographs in 1960. Evidence for binary fission of the bacteria, their phagocytosis by histiocytes in the lamina propria, their occurrence between and within the cells of the epithelium and on the brush border of the lumen were reported later. Scanning electron microscopy has been applied by us in an attempt to confirm the earlier observations by the new technique and to describe the bacterium further. Both transmission and scanning electron microscopy have been used concurrently to study the same biopsy specimens, and transmission observations have been used to confirm those made by scanning.The locations of the brush borders, the columnar epithelial cells, the basement membrane and the lamina propria beneath it were each easily identified by scanning electron microscopy. The lamina propria was completely filled with the wiener-shaped bacteria, Fig. 1.

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