Sensitivity Testing and Mic Determination of Obligate Anaerobes

1987 ◽  
pp. 28-29
Author(s):  
D. Felmingham
Keyword(s):  
Sensitivity Testing

scholarly journals In-vivo Anti-plasmodial Activity of Ethanol Tetracarpidium conophorum Seed Extract and Its Anti-typhoidal Activity In-vitro

2021 ◽  
pp. 35-42
Author(s):  
Faith Temiloluwa Oladipo ◽  
Ebenezer Oluyemi Dada
Keyword(s):  
Salmonella Typhimurium ◽  
Antibiotic Sensitivity ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Seed Extract ◽  
Sensitivity Testing ◽  
Group 2

Aim: This study was conducted to assess the in-vivo anti-plasmodial activity of ethanol Tetracarpidium conophorum seed extract and its anti-typhoidal activity in-vitro. Study Design: Experimental study. Methodology: Standard methods were conducted to determine the acute toxicity test of Tetracarpidium conophorum seed ethanol extract, determination of Body Weight and Temperature of Mice, determination of antibacterial activity of Tetracarpidium conophorum seed crude extract and antibiotic sensitivity testing of clinical and typed Salmonella Typhi isolates Results: There were no signs of toxicity such as paw licking, sleeping, reduced activity,             respiratory distress observed in mice and there were no mortality. Changes occurred in the              weight of mice in group 1 (mice treated with 200mg/kg) from 19.71g to 14.50g wherein significant increment was observed only from day 3 to 4 (18.67g to 16.00g), group 2 (mice treated                       with 400mg/kg) 19.71g to 16.00g). The ethanol extract of Tetracapidium conophorum were all resistant to typed isolates of Salmonella typhimurium and clinical isolates of Salmonella typhimurium at 6.01±0.10 mm. Ciprofloxacin (5 µg) had the highest zone of inhibition at 32.50±2.50 mm against typed Salmonella typhimurium while tetracycline (30 µg) had the least at 15.50±0.50 mm. Conclusion: This study has revealed the anti-plasmodial efficacy of Tetracarpidium conophorum seed. Findings have shown that the ethanol seed extract of T. conophorum relatively possess anti-plasmodial and anti-typhodial activities compared to the positive antibiotic susceptibility of Salmonella typhi. Auxiliary evaluation must be conducted to establish the anti-typhoidal activity of ethanol T. conophorum extract in-vivo.

Contribution of organic bee pollen to the determination of the botanical origin of honey and its impact on its biological properties

2021 ◽  
Vol 18 ◽  
Author(s):  
Amar Otmani ◽  
Nadia Amessis-Ouchemoukh ◽  
Zina Mouhoubi-Tafinine ◽  
Kenza Tighlit ◽  
Ibtissam Redouan ◽  
...  
Keyword(s):  
Physicochemical Parameters ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Biological Properties ◽  
Total Phenolic ◽  
Botanical Origin ◽  
Sensitivity Testing ◽  
Physico Chemical

Background: Honey is a sweet and flavorful natural product which comes from a lot of nectar of medicinal plants. Objective: The aims of this study were to determine the botanical origin, physicochemical parameters and the antioxidant contents and antibacterial activities of some Algerian honeys. Method: The physico-chemical parameters of the honeys analyzed were measured using the methods established by the European Honey Commission. The antioxidant contents were determined using colorimetric assays, and the antioxidant activities were estimated using reducing power, DPPH, ABTS, and FRAP assays. Antibacterial activities were measured using sensitivity testing and minimal inhibitory concentration. Results: The majority of the samples analyzed were monofloral honeys (Fabaceae) and other medicinal plant pollens were shown (Asteraceae, Tiliaceae, Myrtaceae and Apiaceae). The physicochemical parameters of the honeys were in accordance with the legislation, and the analyses of proline and HMF confirmed their authenticity. The content of total phenolic compounds and total flavonoids ranged from 26 to 159 mg GAE/100 g and 10 to 43 mg EC/100 g, respectively. The analyzed honeys showed variable antioxidant activities that differed from one honey to another, and antibacterial activity tests showed that S. aureus and K. pneumoniae were the most sensitive strains with inhibition zones of 24 to 28 mm and 8 to 35 mm in diameter, respectively. Very high correlations were observed between color, antioxidants and antioxidant activities. Conclusion: This study confirmed the powerful properties of honey to trap free radicals and to inhibit bacterial growth that could be used as a therapeutic agent.

scholarly journals Field-Based Flow Cytometry forEx VivoCharacterization of Plasmodium vivax and P. falciparum Antimalarial Sensitivity

2013 ◽  
Vol 57 (10) ◽  
pp. 5170-5174 ◽  
Author(s):  
B. Russell ◽  
B. Malleret ◽  
R. Suwanarusk ◽  
C. Anthony ◽  
S. Kanlaya ◽  
...  
Keyword(s):  
Plasmodium Vivax ◽  
Drug Sensitivity ◽  
Ex Vivo ◽  
Low Cost ◽  
Sensitivity Testing ◽  
Microscopic Method ◽  
Content Type ◽  
Optimized Protocol ◽  
Objective Determination

ABSTRACTEx vivoantimalarial sensitivity testing in human malaria parasites has largely depended on microscopic determination of schizont maturation. While this microscopic method is sensitive, it suffers from poor precision and is laborious. The recent development of portable, low-cost cytometers has allowed us to develop and validate a simple, field-optimized protocol using SYBR green and dihydroethidium for the accurate and objective determination of antimalarial drug sensitivity in freshly isolatedPlasmodium vivaxandPlasmodium falciparum.

scholarly journals Study of antibiotic sensitivity of Esherichia coli isolated from Broiler Chickens

2018 ◽  
Vol 11 (2) ◽  
Author(s):  
S Hussein ◽  
M AL-Attar
Keyword(s):  
Minimal Inhibitory Concentration ◽  
Broiler Chickens ◽  
Inhibitory Concentration ◽  
Antibiotic Sensitivity ◽  
Resistance Pattern ◽  
Multiple Resistance ◽  
Sensitivity Testing ◽  
Esherichia Coli ◽  
Complete Resistance

Antibiotic sensitivity testing of E.coli isolates, showed that most isolates were sensitive 100% to colistin sulphate and kanamycin/cephlaxine in the first degree, also gave different results to other antibiotics. On the other hand, the isolates showed complete resistance 100% for Ampicillin, Gentamycin, Pencillin G, Tetracycline and Tylosin, in addition to appearance of 11 multiple resistance pattern. The results of determination of minimal inhibitory concentration (MIC) for E.coli showed the presence of variable minimal inhibitory concentration in relation to the serotypes of E.coli when used different antibiotics.

scholarly journals Determination of drug resistance of Mycobacterium tuberculosis in the operating material

2021 ◽  
Vol 69 (1) ◽  
Author(s):  
Maria Uraksina ◽  
◽  
Petr Rogozhkin ◽  
Ekaterina Eremenko ◽  
◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Drug Sensitivity ◽  
Molecular Genetic ◽  
Multi Drug Resistance ◽  
Sensitivity Testing ◽  
Number Of Patients ◽  
Primary Drug Resistance ◽  
Extensively Drug Resistance

Currently, with the improvement of epidemic indicators for tuberculosis, the number of patients with drug-resistant forms of tuberculosis is increasing, which complicates and prolongs the treatment of such patients. The aim of the work is to study the drug resistance of Mycobacterium tuberculosis obtained from surgical material. Materials and methods: the study included 74 patients with medical and diagnostic operations on the chest organs. All patients were examined by standard methods: sputum and BAL microscopy with bronchoscopy (during the procedure), molecular genetic methods, culture on dense and liquid nutrient media. The diagnosis of tuberculosis was confirmed morphologically in all patients by histological examination of surgical material. When analyzing the results of drug resistance, it was found that among the newly identified patients, more than half (59.3%) had multiple (44.6%) p<0.005 and extensively drug resistance (14.7%) p= 0.003. At the same time, 74.5% (n= 35/47) of patients had no MBT in sputum before surgery. The frequency of development of drug resistance to first-and reserve-line antibacterial drugs, the structure of drug resistance depending on the group of dispensary registration is presented. A comparative analysis of drug sensitivity testing of MBT isolated from sputum before surgery and from surgical material was performed. The results of the study showed a high percentage of MBT drug resistance obtained in the operating material in patients with negative MBT tests. More than half of the patients who did not receive antibacterial therapy before surgery have multi drug resistance and extensively drug resistance, which suggests a high regional primary drug resistance of Mycobacterium tuberculosis.

Galactic orbits of stars

1966 ◽  
Vol 25 ◽  
pp. 93-97
Author(s):  
Richard Woolley
Keyword(s):  
Globular Cluster ◽  
Potential Field ◽  
High Velocity ◽  
Velocity Vector ◽  
Variable Stars ◽  
The Sun ◽  
Proper Motions ◽  
Rr Lyrae ◽  
The Galaxy

It is now possible to determine proper motions of high-velocity objects in such a way as to obtain with some accuracy the velocity vector relevant to the Sun. If a potential field of the Galaxy is assumed, one can compute an actual orbit. A determination of the velocity of the globular clusterωCentauri has recently been completed at Greenwich, and it is found that the orbit is strongly retrograde in the Galaxy. Similar calculations may be made, though with less certainty, in the case of RR Lyrae variable stars.

Distance to SN 1987A and the LMC

1999 ◽  
Vol 190 ◽  
pp. 549-554
Author(s):  
Nino Panagia
Keyword(s):  
Angular Size ◽  
Large Magellanic Cloud ◽  
Magellanic Cloud ◽  
Light Curves ◽  
Distance Modulus ◽  
Absolute Size ◽  
Sn 1987A

Using the new reductions of the IUE light curves by Sonneborn et al. (1997) and an extensive set of HST images of SN 1987A we have repeated and improved Panagia et al. (1991) analysis to obtain a better determination of the distance to the supernova. In this way we have derived an absolute size of the ringRabs= (6.23 ± 0.08) x 1017cm and an angular sizeR″ = 808 ± 17 mas, which give a distance to the supernovad(SN1987A) = 51.4 ± 1.2 kpc and a distance modulusm–M(SN1987A) = 18.55 ± 0.05. Allowing for a displacement of SN 1987A position relative to the LMC center, the distance to the barycenter of the Large Magellanic Cloud is also estimated to bed(LMC) = 52.0±1.3 kpc, which corresponds to a distance modulus ofm–M(LMC) = 18.58±0.05.

International determination of the total motion of the pole

1961 ◽  
Vol 13 ◽  
pp. 29-41
Author(s):  
Wm. Markowitz
Keyword(s):  
Secular Motion ◽  
The Future

A symposium on the future of the International Latitude Service (I. L. S.) is to be held in Helsinki in July 1960. My report for the symposium consists of two parts. Part I, denoded (Mk I) was published [1] earlier in 1960 under the title “Latitude and Longitude, and the Secular Motion of the Pole”. Part II is the present paper, denoded (Mk II).

Time and Latitude in South Africa

1972 ◽  
Vol 1 ◽  
pp. 27-38
Author(s):  
J. Hers
Keyword(s):  
South Africa ◽  
Cape Town ◽  
Astronomical Observations ◽  
Royal Observatory ◽  
The Republic ◽  
Astronomical Determination ◽  
Limited Accuracy ◽  
Better Than

In South Africa the modern outlook towards time may be said to have started in 1948. Both the two major observatories, The Royal Observatory in Cape Town and the Union Observatory (now known as the Republic Observatory) in Johannesburg had, of course, been involved in the astronomical determination of time almost from their inception, and the Johannesburg Observatory has been responsible for the official time of South Africa since 1908. However the pendulum clocks then in use could not be relied on to provide an accuracy better than about 1/10 second, which was of the same order as that of the astronomical observations. It is doubtful if much use was made of even this limited accuracy outside the two observatories, and although there may – occasionally have been a demand for more accurate time, it was certainly not voiced.

Large-scale Motion of Solar Filaments

2000 ◽  
Vol 179 ◽  
pp. 205-208
Author(s):  
Pavel Ambrož ◽  
Alfred Schroll
Keyword(s):  
Magnetic Flux ◽  
Proper Motion ◽  
Time Scale ◽  
Large Scale ◽  
Accuracy Of Measurements ◽  
Solar Filaments ◽  
General Movement ◽  
Scale Motion ◽  
Velocity Scatter

AbstractPrecise measurements of heliographic position of solar filaments were used for determination of the proper motion of solar filaments on the time-scale of days. The filaments have a tendency to make a shaking or waving of the external structure and to make a general movement of whole filament body, coinciding with the transport of the magnetic flux in the photosphere. The velocity scatter of individual measured points is about one order higher than the accuracy of measurements.

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