Contribution of organic bee pollen to the determination of the botanical origin of honey and its impact on its biological properties

2021 ◽  
Vol 18 ◽  
Author(s):  
Amar Otmani ◽  
Nadia Amessis-Ouchemoukh ◽  
Zina Mouhoubi-Tafinine ◽  
Kenza Tighlit ◽  
Ibtissam Redouan ◽  
...  
Keyword(s):  
Physicochemical Parameters ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Biological Properties ◽  
Total Phenolic ◽  
Botanical Origin ◽  
Sensitivity Testing ◽  
Physico Chemical

Background: Honey is a sweet and flavorful natural product which comes from a lot of nectar of medicinal plants. Objective: The aims of this study were to determine the botanical origin, physicochemical parameters and the antioxidant contents and antibacterial activities of some Algerian honeys. Method: The physico-chemical parameters of the honeys analyzed were measured using the methods established by the European Honey Commission. The antioxidant contents were determined using colorimetric assays, and the antioxidant activities were estimated using reducing power, DPPH, ABTS, and FRAP assays. Antibacterial activities were measured using sensitivity testing and minimal inhibitory concentration. Results: The majority of the samples analyzed were monofloral honeys (Fabaceae) and other medicinal plant pollens were shown (Asteraceae, Tiliaceae, Myrtaceae and Apiaceae). The physicochemical parameters of the honeys were in accordance with the legislation, and the analyses of proline and HMF confirmed their authenticity. The content of total phenolic compounds and total flavonoids ranged from 26 to 159 mg GAE/100 g and 10 to 43 mg EC/100 g, respectively. The analyzed honeys showed variable antioxidant activities that differed from one honey to another, and antibacterial activity tests showed that S. aureus and K. pneumoniae were the most sensitive strains with inhibition zones of 24 to 28 mm and 8 to 35 mm in diameter, respectively. Very high correlations were observed between color, antioxidants and antioxidant activities. Conclusion: This study confirmed the powerful properties of honey to trap free radicals and to inhibit bacterial growth that could be used as a therapeutic agent.

Optimization of Polyphenol Extraction from Agaricus blazei Murrill and Determination of its Antioxidant Activities

2019 ◽  
Vol 18 (2) ◽  
pp. 158-165
Author(s):  
Wei Qi ◽  
Zhan Yi-shu ◽  
Chen Bing-zhi ◽  
Jiang Yu-ji
Keyword(s):  
Sulfonic Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Agaricus Blazei ◽  
Liquid Ratio ◽  
Edible Fungus

Agaricus blazei Murrill is an edible fungus. It is considered as a functional food and its extract is also used as a natural therapy for human diseases related to metabolism in Asian countries. Polyphenols of ABM are powerful radical scavengers and providing potent antioxidant benefits. This study aimed to optimize the extraction conditions of polyphenols from Agaricus blazei Murrill by response surface methodology and determination of its antioxidant activities. The influence of extraction temperature, extraction time, and solid to liquid ratio on each response was investigated. The optimal extract conditions for Agaricus blazei Murrill polyphenols were at 72°C for 2.5 h, and solid to liquid ratio of 1:20. Under the optimal conditions, the total phenolic content of Agaricus blazei Murrill was 2.92 mg/g, which was close to the predicted value (3.04 mg/g). The antioxidant activities of ABM extract were analyzed by 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) and hydroxyl radical scavenging activities and the reducing power. The EC50 values of Agaricus blazei Murrill extract on 1,1-diphenyl-2-picrylhydrazyl, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid), and hydroxyl radicals were 0.44, 0.30, and 2.84 mg/mL, respectively. The findings indicated that Agaricus blazei Murrill extract provide a beneficial effect on antioxidant activity. It suggests that Agaricus blazei Murrill has the potential to be a valuable source of natural antioxidant.

Antioxidant and Antibacterial Activities of Phytochemicals in Methanolic Extracts of Five Underutilized Leafy Vegetables

2021 ◽  
Vol 16 (8) ◽  
pp. 1-10
Author(s):  
Adarsha Raj ◽  
Bratati Sikdar ◽  
Alokesh Roy ◽  
Asish Kumar Mukhopadhyay ◽  
Sudipta Roy
Keyword(s):  
Antioxidant Activities ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
Antibacterial Activities ◽  
Leafy Vegetables ◽  
Alternanthera Philoxeroides ◽  
Total Phenolic ◽  
Bacterial Strains ◽  
Methanolic Extracts ◽  
Therapeutic Uses

Leafy vegetables are important for their nutritive and medicinal values. However, many of them are less explored and underutilized. The present study evaluates the antioxidant and antibacterial activities present in five underutilized leafy vegetables such as Alternanthera philoxeroides, Boerhavia diffusa, Enydra(=Enhydra) fluctuans, Glinus oppositifolius and Suaeda maritima. Methanolic extracts from leafy edible parts of these plants possessed diverse phytochemicals. Of these plants, the polyphenol contents like total phenolic (TPC), total flavonoid (TFC) and total tannin (TTC) were the highest in E. fluctuans with significant variations concerning other plants. Also, the antioxidant activities of E. fluctuans were greater comparing other plants; the parameters were the total antioxidant activity (TAA), ferric reducing power assay and ability to scavenge DPPH, ABTS and superoxide radicals. The effect of varying extract-quantities revealed the highest antibacterial activity of the E. fluctuans extracts with substantial inhibition zones against six bacterial strains. Accordingly, the E. fluctuans extracts showed the lowest MIC (2.75±0.35 mg ml-1) and MBC (3.50±0.71 mg ml-1), albeit with significant variations with ampicillin. The study highlights that these plants are the untapped repertoires of natural antioxidants and antimicrobials for dietary and therapeutic uses.

scholarly journals Determination of Multiple Antioxidant Activities of Endemic Tricholoma anatolicum H.H Doğan & Intini Collected from Turkey

2018 ◽  
Vol 6 (11) ◽  
pp. 1582 ◽  
Author(s):  
Hatıra Taşkın ◽  
Tülin Eker ◽  
Fuat Bozok ◽  
Hasan Hüseyin Doğan ◽  
Saadet Büyükalaca
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Far East ◽  
Reducing Power ◽  
Total Phenolic ◽  
Mushroom Species ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

Tricholoma anatolicum H.H. Doğan & Intini is known as the most prized mushroom species in Feke region of Turkey. This mushroom species is collected from Cedar (Cedrus libani) forests and therefore is named as Cedar mushroom in this region. It is collected and consumed by local collectors and also exported to Far East countries such as Japan by exporting companies. Therefore, it is important to investigate the nutritional and medical importance of this species. The aim of this study is to reveal the antioxidant activity of methanol extract in different concentrations (1, 2 and 4 mg/mL) of T. anatolicum H.H. Doğan & Intini collected from Feke district of Adana province of Turkey in 2015. In this study, it was determined that total phenolic content of this mushroom was 56 mg/kg. 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO) and reducing power (RP) activities given in trolox (µM) and ferric-reducing antioxidant power (FRAP) given in FeSO4 (µM) were found as 449, 180, 337 and 2 at the highest concentration (4 mg/mL), respectively. As a result, it could be suggested that methanol extract of T. anatolicum H.H. Doğan & Intini has significantly antioxidant activity.

Quantification of Total Flavonoids, Total Phenolic Compounds and Antioxidant Activity of Extracts from the Seed of Viburnum odoratissimum

2013 ◽  
Vol 709 ◽  
pp. 879-882
Author(s):  
Shu Mei Qiu ◽  
Hai Lan Huang ◽  
Hai Yuan Wang ◽  
Zong Hua Wang
Keyword(s):  
Superoxide Radical ◽  
Soluble Fraction ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Viburnum Odoratissimum

The fruit of Viburnum odoratissimum is an elliptical berry. The aims of our study were the quantification of some antioxidants using colorimetric assays and the determination of antioxidant activities by three methods, and the relationships between these bioactive compounds and antioxidant activities of the crude extract (CE) and its four fractions derived from the seed of the fruit of Viburnum odoratissimum were investigated. The results showed that the n-butanol-soluble fraction (BF)was the richest fraction in total flavonoids and total phenolic. The ethyl acetate-soluble fraction (EAF) and BF showed good superoxide radical scavenging activity(SRSA) and reducing power(RP), while the petroleum ether-soluble fraction(PEF) possessed the highest inhibiting lipid peroxidantion activity(LPA).There were statistically significant correlations between some antioxidants and antioxidant activities.

Comparison Among Five Eucalyptus Species Based on Their Leaf Contents of Some Primary and Secondary Metabolites

2019 ◽  
Vol 20 (7) ◽  
pp. 573-587
Author(s):  
Alyaa Nasr ◽  
Tehmina Saleem Khan ◽  
Shi-Ping Huang ◽  
Bin Wen ◽  
Jian-Wen Shao ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Protocatechuic Acid ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Renewable Resource ◽  
Total Phenolic ◽  
Eucalyptus Species ◽  
Primary And Secondary Metabolites ◽  
Acetone Water ◽  
Phenolic Constituents

Background: Eucalyptus belongs to the Myrtaceae family. It is the most planted hardwood forest crop worldwide, representing a global renewable resource of fiber, pharmaceuticals and energy. Objective: To compare the five species, E. maidenii, E. robusta, E. citriodora, E. tereticornis and E. camaldulensis, seeking for the richest source of nutrients and pharmaceuticals. Methodology: Eucalyptus samples were subjected to some chemical determinations for both primary and secondary metabolites to verify their nutritional and pharmaceutical importance related to different extracts. GC-MS analysis was applied to detect the presence of some individual phenolic constituents in their leaves. Results: E. robusta recorded the maximum contents of carbohydrates (40.07%) and protein (31.91%). While E. camaldulensis contained the highest contents of total phenolic compounds (46.56 mg/g), tannins (40.01 mg/g) and antioxidant activities assayed by the phosphomolybednum method (57.60 mg/g), followed by E. citridora. However, E. tereticornis exhibited the highest reducing power ability (151.23 mg/g). The GC-MS highlighted 20 phenolic constituents and antioxidants which varied in their abundance in Eucalyptus leaves, 8 individual phenolics (hydroquinone, hesperitin, pyrogallol, resorcinol, protocatechuic acid, naringenin, chlorogenic acid and catechin) were maximally recorded with E. camaldulensis and secondly, with E. citridora in case of at least 5 components. Nevertheless, gallic and quinic acids were more abundant in the leaves of E. tereticornis, which may explain its high corresponding reducing powers. Conclusion: Acetone-water combination has enhanced phenolics extraction from Eucalyptus tissues. This is the first report aiming to compare between the aforementioned Eucalyptus species highlighting either their nutritional or medicinal importance.

Development and Pharmacokinetics Study of Antifungal Peptide Nanoliposomes by Liquid Chromatography-tandem Mass Spectrometry

2019 ◽  
Vol 15 (4) ◽  
pp. 312-318
Author(s):  
Shuoye Yang
Keyword(s):  
Mass Spectrometry ◽  
Biological Properties ◽  
Pharmacokinetic Property ◽  
Antifungal Peptide ◽  
Simple Liquid ◽  
Physico Chemical ◽  
Liquid Chromatography Tandem Mass ◽  
Pegylated Lipids

Background: The therapeutic ability and application of antifungal peptide (APs) are limited by their physico-chemical and biological properties, the nano-liposomal encapsulation would improve the in vivo circulation and stability. </P><P> Objective: To develop a long-circulating liposomal delivery systems encapsulated APs-CGA-N12 with PEGylated lipids and cholesterol, and investigated through in vivo pharmacokinetics. Methods: The liposomes were prepared and characterized, a rapid and simple liquid chromatographytandem mass spectrometry (LC-MS/MS) assay was developed for the determination of antifungal peptide in vivo, the pharmacokinetic characteristics of APs liposomes were evaluated in rats. Results: Liposomes had a large, unilamellar structure, particle size and Zeta potential ranged from 160 to 185 nm and -0.55 to 1.1 mV, respectively. The results indicated that the plasma concentration of peptides in reference solutions rapidly declined after intravenous administration, whereas the liposomeencapsulated ones showed slower elimination. The AUC(0-∞) was increased by 3.0-fold in liposomes in comparison with standard solution (20 mg·kg-1), the half-life (T1/2) was 1.6- and 1.5-fold higher compared to the reference groups of 20 and 40 mg·kg-1, respectively. Conclusion: Therefore, it could be concluded that liposomal encapsulation effectively improved the bioavailability and pharmacokinetic property of antifungal peptides.

scholarly journals Antioxidant and Anticancer Activities of Wampee (Clausena lansium(Lour.) Skeels) Peel

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
K. Nagendra Prasad ◽  
Jing Hao ◽  
Chun Yi ◽  
Dandan Zhang ◽  
Shengxiang Qiu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Liver Carcinoma ◽  
Scavenging Activity ◽  
Anticancer Activities

Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water) were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF) exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT). In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901), human hepatocellular liver carcinoma (HepG-2) and human lung adenocarcinoma (A-549) cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

Polyphenol Contents, Antioxidant and Antibacterial Activities of Aqueous Algerian Propolis Extracts

2021 ◽  
Author(s):  
E.K. Soltani ◽  
K. Zaim ◽  
K. Mokhnache ◽  
N. Haichour ◽  
S. Mezaache-Aichour ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Natural Antioxidants ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Biological Properties ◽  
Aqueous Extracts

The propolis, an extremely complex resinous material, exhibits valuable pharmacological and biological properties, mainly attributed to the presence of polyphenols. The composition of propolis depends on time, vegetation, and the area of collection. Total flavonoid and polyphenol contents of aqueous extracts of propolis samples from different areas of Algeria, determined by using aluminum chloride and Folin–Ciocalteu colorimetric methods, were in the range of 3.047 ± 0.004–5.273 ± 0.013 mg/g and 96.833 ± 0.027–458.833 ± 0.0005 mg/g crude extract of propolis, respectively. This study examined the antioxidant and antimicrobial activities of propolis. Aqueous extracts of propolis were obtained in order to evaluate their antioxidant activities by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, β-carotene and electrochemical assays. All tested propolis samples had relatively strong antioxidant activities, which were also correlated with the total polyphenol and flavonoid content present. The percentage of inhibition of lipid peroxidation of linoleic acid emulsion during 24 h varied between 86.04 ± 0.42 and 90.60 ± 3.77% among the tested samples. The highest DPPH radical scavenging activity was observed by ABAL (Ain Abassa Aqueous Extract) with IC50 = 8.49 ± 5.07 10−5 μg/ml, and the lowest was observed by SAL (Setif Aqueous Extract) with IC50 of 21.16 ± 0.0001 μg/ml. The most important antibacterial activity was obtained with Ain Abassa extract; the zones of inhibition obtained for this excerpt vary from 15.22 to 15.5 mm. Followed by the Setif extract with areas of 12.33 to 12.75 mm, the Tizi-Ouzou extract with areas of 10.11 to 11.11 mm. This study will bring an innovation for further studies with regard to the antioxidant and antibacterial properties of the aqueous extracts of propolis. This study corroborates that Algerian propolis is a rich source of natural antioxidants, properties which could be used in the prevention of different diseases, both in humans and in animals.

scholarly journals FTIR PHYTOCHEMICAL FINGERPRINTING AND ANTIOXIDANT ANLYSES OF SELECTED INDOOR NON-FLOWERING INDOOR PLANTS AND THEIR INDUSTRIAL IMPORTANCE

2016 ◽  
Vol 8 (4) ◽  
pp. 37
Author(s):  
Jayaprada Rao Chunduri ◽  
Hetwi R. Shah
Keyword(s):  
Reducing Power ◽  
Antibiotic Sensitivity ◽  
Antibacterial Activities ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Ph Indicator ◽  
Sensitivity Testing ◽  
Indoor Plants ◽  
The Rich ◽  
High Concentrations

Objective: phytochemical screening and antioxidant activity analyses of selected indoor plants and to evaluate commercial applications.Methods: Qualitative and quantitative phytochemical analyses of alcoholic and aquatic crude extracts of leaves of selected non-flowering indoor plants were assessed using standard protocols and later compared with FTIR analyses. Antioxidant and antibacterial activities of the extracts were studiedResults: Phytochemical analysis of polar solvent extractions of the four selected plants Pedilanthus tithymaloides, Cordyline terminalis, Tradescantia zebrine and Rhoeo discolou. Indicated the presence of tannins in all four varieties terpenoids in 3, flavonols, phytosterols and phenols in two plants, followed by alkaloids. The phytochemical analyses were supported by FTIR reports. Quantitative studies indicated variations in flavonol, tannin and phenols concentrations among the four species. High concentrations of Total flavonols (P. tithymaloides) and Tannins (C. terminalis) were observed. C. terminalis extract showed comparatively highest reducing power followed by R. discolour and P. tithymaloides extracts. Antibiotic Sensitivity Testing indicated P. tithymaloides showed a maximum zone of inhibition compared to R. discolor. C. terminalis plant leaf extract showed a faint zone of inhibition against E. fecalis while others couldnot. Intense colors of C. terminalis and T. zebrine plants could be used as a natural dye as well as pH indicator. Conclusion: The rich concentrations of the tannins from non-flowering indoor plants could be the future option of dyes and dyeing industry as natural colorants as well as pH indicators. These plants were rich sources of phytochemicals (phenols, flavonols, tannins, and phytosterols), with antioxidant and antibacterial activity.

scholarly journals Phytochemical Screening, Polyphenols Content, Antioxidant Power, and Antibacterial Activity of Herniaria hirsuta from Morocco

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Kenza Ammor ◽  
Dalila Bousta ◽  
Sanae Jennan ◽  
Bahia Bennani ◽  
Abdellah Chaqroune ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Antioxidant Power ◽  
Negative Effect ◽  
Hydroethanolic Extract

The aim of this study is to investigate in vitro antioxidant and antibacterial activities of the aqueous and hydroethanolic extracts for aerial parts of Herniaria hirsuta. Extracts were screened for their possible antioxidant activities by three tests: DPPH free radical-scavenging, reducing power, and molybdenum systems. The screening of antibacterial activity of extracts was individually evaluated against sixteen bacteria species using a disc diffusion method. Flavonoids, total phenols, and tannins content were performed for both extracts. It shows higher content in the hydroethanolic extract. The hydroethanolic extract showed a significant antioxidant activity for the three methods studies to the aqueous extract, but nonsignificant results compared to the reference (BHT). However, both extracts have negative effect on the strains studies for the antibacterial activity.

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