Detoxification of Mycotoxins In Planta as a Strategy for Improving Grain Quality and Disease Resistance: Identification of Fumonisin-Degrading Microbes from Maize

1998 ◽  
pp. 369-381 ◽  
Author(s):  
Jon Duvick ◽  
Tracy Rood ◽  
Joyce Maddox ◽  
Jay Gilliam
Keyword(s):  
Disease Resistance ◽  
Grain Quality ◽  
In Planta

‘Billings’ Wheat Combines Early Maturity, Disease Resistance, and Desirable Grain Quality for the Southern Great Plains, USA

2013 ◽  
Vol 8 (1) ◽  
pp. 22-31 ◽  
Author(s):  
Robert M. Hunger ◽  
Jeffrey T. Edwards ◽  
Robert L. Bowden ◽  
Liuling Yan ◽  
Patricia Rayas-Duarte ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Great Plains ◽  
Grain Quality ◽  
Early Maturity ◽  
Southern Great Plains

scholarly journals Effects of rain shelter or simulated rain during grain filling and maturation on subsequent wheat grain quality in the UK

2016 ◽  
Vol 155 (2) ◽  
pp. 300-316 ◽  
Author(s):  
G. YADAV ◽  
R. H. ELLIS
Keyword(s):  
Grain Quality ◽  
Sodium Dodecyl ◽  
Dry Weight ◽  
Grain Filling ◽  
Quality Trait ◽  
In Planta ◽  
N Concentration ◽  
The Uk ◽  
Late Maturation ◽  
Harvest Maturity

SUMMARYThe effects of simulated additional rain (ear wetting, 25 mm) or of rain shelter imposed at different periods after anthesis on grain quality at maturity and the dynamics of grain filling and desiccation were investigated in UK field-grown crops of wheat (Triticum aestivum L., cvar Tybalt) in 2011 and in 2012 when June–August rainfall was 255·0 and 214·6 mm, respectively, and above the decadal mean (157·4 mm). Grain filling and desiccation were quantified well by broken-stick regressions and Gompertz curves, respectively. Rain shelter for 56 (2011) or 70 days (2012) after anthesis, and to a lesser extent during late maturation only, resulted in more rapid desiccation and hence progress to harvest maturity whereas ear wetting had negligible effects, even when applied four times. Grain-filling duration was also affected as above in 2011, but with no significant effect in 2012. In both years, there were strong positive associations between final grain dry weight and duration of filling. The treatments affected all grain quality traits in 2011: nitrogen (N) and sulphur (S) concentrations, N : S ratio, sodium dodecyl sulphate (SDS) sedimentation volume, Hagberg Falling Number (HFN), and the incidence of blackpoint. Only N concentration and blackpoint were affected significantly by treatments in 2012. Rain shelter throughout grain filling reduced N concentration, whereas rain shelter reduced the incidence of blackpoint and ear wetting increased it. In 2011, rain shelter throughout reduced S concentration, increased N : S ratio and reduced SDS. Treatment effects on HFN were not consistent within or between years. Nevertheless, a comparison between the extreme treatment means in 2012 indicated damage from late rain combined with ear wetting resulted in a reduction of c. 0·7 s in HFN/mm August rainfall, while that between samples taken immediately after ear wetting at harvest maturity or 7 days later suggested recovery from damage to HFN upon re-drying in planta. Hence, the incidence of blackpoint was the only grain quality trait affected consistently by the diverse treatments. The remaining aspects of grain quality were comparatively resilient to rain incident upon developing and maturing ears of cvar Tybalt. No consistent temporal patterns of sensitivity to shelter or ear wetting were detected for any aspect of grain quality.

scholarly journals N-hydroxy-pipecolic acid is a mobile signal that induces systemic disease resistance in Arabidopsis

2018 ◽  
Author(s):  
Yun Chu Chen ◽  
Eric C. Holmes ◽  
Jakub Rajniak ◽  
Jung-Gun Kim ◽  
Sandy Tang ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Systemic Acquired Resistance ◽  
Systemic Disease ◽  
Acquired Resistance ◽  
Bacterial Pathogen ◽  
Pipecolic Acid ◽  
In Planta ◽  
Global Response ◽  
Promising Target ◽  
Pathogenesis Related

AbstractSystemic acquired resistance (SAR) is a global response in plants induced at the site of infection that leads to long-lasting and broad-spectrum disease resistance at distal, uninfected tissues. Despite the importance of this priming mechanism, the identity of the mobile defense signal that moves systemically throughout plants to initiate SAR has remained elusive. In this paper, we describe a new metabolite, N-hydroxy-pipecolic acid (N-OH-Pip), and provide evidence that this molecule is a mobile signal that plays a central role in initiating SAR signal transduction in Arabidopsis thaliana. We demonstrate that FLAVIN-DEPENDENT MONOOXYGENASE 1 (FMO1), a key regulator of SAR-associated defense priming, can synthesize N-OH-Pip from pipecolic acid in planta, and exogenously applied N-OH-PIP moves systemically in Arabidopsis and can rescue the SAR-deficiency of fmo1 mutants. We also demonstrate that N-OH-Pip treatment causes systemic changes in the expression of pathogenesis-related genes and metabolic pathways throughout the plant, and enhances resistance to a bacterial pathogen. This work provides new insight into the chemical nature of a mobile signal for SAR and also suggests that the N-OH-Pip pathway is a promising target for metabolic engineering to enhance disease resistance.

scholarly journals GT Factor ZmGT-3b Is Associated With Regulation of Photosynthesis and Defense Response to Fusarium graminearum Infection in Maize Seedling

2021 ◽  
Vol 12 ◽  
Author(s):  
Qianqian Zhang ◽  
Tao Zhong ◽  
Lizhu E ◽  
Mingliang Xu ◽  
Weixing Dai ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Plant Growth ◽  
Fusarium Graminearum ◽  
Seedling Growth ◽  
Defense Response ◽  
Resistance Mechanism ◽  
Defense Responses ◽  
In Planta ◽  
Trade Off ◽  
Tf Gene

It is of critical importance for plants to correctly and efficiently allocate their resources between growth and defense to optimize fitness. Transcription factors (TFs) play crucial roles in the regulation of plant growth and defense response. Trihelix TFs display multifaceted functions in plant growth, development, and responses to various biotic and abiotic stresses. In our previous investigation of maize stalk rot disease resistance mechanism, we found a trihelix TF gene, ZmGT-3b, which is primed for its response to Fusarium graminearum challenge by implementing a rapid and significant reduction of its expression to suppress seedling growth and enhance disease resistance. The disease resistance to F. graminearum was consistently increased and drought tolerance was improved, while seedling growth was suppressed and photosynthesis activity was significantly reduced in the ZmGT-3b knockdown seedlings. Thus, the seedlings finally led to show a kind of growth–defense trade-off phenotype. Moreover, photosynthesis-related genes were specifically downregulated, especially ZmHY5, which encodes a conserved central regulator of seedling development and light responses; ZmGT-3b was confirmed to be a novel interacting partner of ZmHY5 in yeast and in planta. Constitutive defense responses were synchronically activated in the ZmGT-3b knockdown seedlings as many defense-related genes were significantly upregulated, and the contents of major cell wall components, such as lignin, were increased in the ZmGT-3b knockdown seedlings. These suggest that ZmGT-3b is involved in the coordination of the metabolism during growth–defense trade-off by optimizing the temporal and spatial expression of photosynthesis- and defense-related genes.

scholarly journals Verticillium dahliae effector VDAL protects MYB6 from degradation by interacting with PUB25/26 E3 ligases for enhancing Verticillium wilt resistance in Arabidopsis

2021 ◽  
Author(s):  
Zhizhong Gong ◽  
Junsheng Qi ◽  
Aifang Ma ◽  
Dingpeng Zhang ◽  
Guangxing Wang ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Plant Resistance ◽  
Plant Disease Resistance ◽  
Plant Disease ◽  
Effector Proteins ◽  
In Planta ◽  
E3 Ligases

Verticillium wilt is a severe plant disease, increasing the plant resistance to this disease is a critical challenge worldwide. Here, we report that the Verticillium dahliae (V. dahliae)-secreted Aspf2-like protein VDAL causes leaf wilting when applied to cotton leaves in vitro, but enhances the resistance to V. dahliae when overexpressed in Arabidopsis or cotton. VDAL interacts with Arabidopsis E3 ligases PUB25 and PUB26 (PUBs) and is ubiquitinated by PUBs in vitro. However, VDAL is not degraded by PUBs in planta. Besides, the pub25 pub26 shows higher resistance to V. dahliae than the wild type. PUBs interact with the transcription factor MYB6 in a yeast two-hybrid screen. MYB6 promotes plant resistance to Verticillium wilt while PUBs ubiquitinate MYB6 and mediate its degradation. VDAL competes with MYB6 for binding to PUBs, and the role of VDAL in increasing wilt disease depends on MYB6. These results suggest that plants evolute a strategy to utilize the invaded effector protein VDAL to resist the V. dahliae infection without causing a hypersensitive response. This study provides the molecular mechanism for plants increasing disease resistance when overexpressing some effector proteins, and may promote searching for more genes from pathogenic fungi or bacteria to engineer plant disease resistance.

Yield improvement and adaptation of wheat to water-limited environments in Australia—a case study

2014 ◽  
Vol 65 (7) ◽  
pp. 676 ◽  
Author(s):  
R. A. Richards ◽  
J. R. Hunt ◽  
J. A. Kirkegaard ◽  
J. B. Passioura
Keyword(s):  
Disease Resistance ◽  
Grain Yield ◽  
Grain Quality ◽  
Management Practices ◽  
Nutrient Management ◽  
Nutrient Use Efficiency ◽  
Grain Legumes ◽  
Yield Potential ◽  
Nutrient Use ◽  
Rainfed Wheat

The improvement in grain yield of wheat throughout Australia through both breeding and management has been impressive. Averaged across all farms, there has been an approximate doubling of yield per unit area since ~1940. This has occurred across a broad range of environments with different rainfall patterns. Interestingly, the gain in the driest years (9 kg ha–1 year–1 or 0.81% year–1) has been proportionally greater than in the most favourable years (13.2 kg ha–1 year–1 or 0.61% per year) when expressed as yield relative to 2012. These data from all farms suggest that further yield progress is likely, and evidence is presented that improved management practices alone could double this rate of progress. The yield increases achieved have been without any known compromise in grain quality or disease resistance. As expected, improvements have come from both changed management and from better genetics, as well as from the synergy between them. Yield improvements due to changed management have been dramatic and are easiest to quantify, whereas those from breeding have been important but more subtle. The management practices responsible have largely been driven by advances in mechanisation that enable direct seeding, more timely and flexible sowing and nutrient management, and improved weed and pest control, many of which have been facilitated by improved crop sequences with grain legumes and oilseeds that improve water- and nutrient-use efficiency. Most of the yield improvements from breeding in Australia have come from conventional breeding approaches where selection is almost solely for grain yield (together with grain quality and disease resistance). Improvements have primarily been through increased harvest index (HI), although aboveground biomass has also been important. We discuss future opportunities to further increase Australian rainfed wheat yields. An important one is earlier planting, which increases resource capture. This will require knowledge of the genes regulating phenological development so that flowering still occurs at the optimum time; appropriate modifications to sowing arrangements and nutrient management will also be required. To improve yield potential, we propose a focus on physiological traits that increase biomass and HI and suggest that there may be more scope to improve biomass than HI. In addition, there are likely to be important opportunities to combine novel management practices with new breeding traits to capture the synergy possible from variety × management interactions. Finally, we comment on research aimed at adapting agriculture to climate change.

scholarly journals Recent advances in cereal breeding.

1982 ◽  
Vol 30 (1) ◽  
pp. 11-23
Author(s):  
F.G.H. Lupton
Keyword(s):  
Disease Resistance ◽  
Grain Quality ◽  
Winter Barley ◽  
Recent Advances ◽  
Cereal Breeding ◽  
The Subject ◽  
Modern Varieties ◽  
Durable Disease Resistance

The subject is discussed under the headings: (1) comparison of old and modern varieties, (2) requirements for further improvement in yield, (3) breeding for durable disease resistance, (4) combination of increased yield with good grain quality, (5) the expansion of winter barley, (6) improvements in the logistics of breeding and (7) longer-term prospects. (Abstract retrieved from CAB Abstracts by CABI’s permission)

Promising rice mutants with high yields, good grain quality, and disease resistance due to irradiation technology

2017 ◽  
Vol 59 (3) ◽  
pp. 48-52
Author(s):  
Duy Duong Tran ◽  
Manh Ha Nguyen ◽  
Duy Quy Tran ◽  
Thi Dung Quan ◽  
Hoang Dung Tran ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Grain Quality ◽  
High Yields ◽  
Irradiation Technology

scholarly journals Disease resistance through impairment of α-SNAP–NSF interaction and vesicular trafficking by soybean Rhg1

2016 ◽  
Vol 113 (47) ◽  
pp. E7375-E7382 ◽  
Author(s):  
Adam M. Bayless ◽  
John M. Smith ◽  
Junqi Song ◽  
Patrick H. McMinn ◽  
Alice Teillet ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Soybean Cyst Nematode ◽  
Protein Complexes ◽  
Essential Gene ◽  
Vesicle Trafficking ◽  
Plant Root ◽  
Wild Type ◽  
In Planta ◽  
Root Cells ◽  
Feeding Site

α-SNAP [soluble NSF (N-ethylmaleimide–sensitive factor) attachment protein] and NSF proteins are conserved across eukaryotes and sustain cellular vesicle trafficking by mediating disassembly and reuse of SNARE protein complexes, which facilitate fusion of vesicles to target membranes. However, certain haplotypes of the Rhg1 (resistance to Heterodera glycines 1) locus of soybean possess multiple repeat copies of an α-SNAP gene (Glyma.18G022500) that encodes atypical amino acids at a highly conserved functional site. These Rhg1 loci mediate resistance to soybean cyst nematode (SCN; H. glycines), the most economically damaging pathogen of soybeans worldwide. Rhg1 is widely used in agriculture, but the mechanisms of Rhg1 disease resistance have remained unclear. In the present study, we found that the resistance-type Rhg1 α-SNAP is defective in interaction with NSF. Elevated in planta expression of resistance-type Rhg1 α-SNAPs depleted the abundance of SNARE-recycling 20S complexes, disrupted vesicle trafficking, induced elevated abundance of NSF, and caused cytotoxicity. Soybean, due to ancient genome duplication events, carries other loci that encode canonical (wild-type) α-SNAPs. Expression of these α-SNAPs counteracted the cytotoxicity of resistance-type Rhg1 α-SNAPs. For successful growth and reproduction, SCN dramatically reprograms a set of plant root cells and must sustain this sedentary feeding site for 2–4 weeks. Immunoblots and electron microscopy immunolocalization revealed that resistance-type α-SNAPs specifically hyperaccumulate relative to wild-type α-SNAPs at the nematode feeding site, promoting the demise of this biotrophic interface. The paradigm of disease resistance through a dysfunctional variant of an essential gene may be applicable to other plant–pathogen interactions.

scholarly journals Mutational Analysis of the Arabidopsis RPS2 Disease Resistance Gene and the Corresponding Pseudomonas syringae avrRpt2 Avirulence Gene

2001 ◽  
Vol 14 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Michael J. Axtell ◽  
Timothy W. McNellis ◽  
Mary Beth Mudgett ◽  
Caroline S. Hsu ◽  
Brian J. Staskawicz
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Pseudomonas Syringae ◽  
Resistance Genes ◽  
Mutational Analysis ◽  
Defense Responses ◽  
Avirulence Gene ◽  
Disease Resistance Gene ◽  
In Planta ◽  
Hypersensitive Cell Death

Plants have evolved a large number of disease resistance genes that encode proteins containing conserved structural motifs that function to recognize pathogen signals and to initiate defense responses. The Arabidopsis RPS2 gene encodes a protein representative of the nucleotide-binding site-leucine-rich repeat (NBS-LRR) class of plant resistance proteins. RPS2 specifically recognizes Pseudomonas syringae pv. tomato strains expressing the avrRpt2 gene and initiates defense responses to bacteria carrying avrRpt2, including a hypersensitive cell death response (HR). We present an in planta mutagenesis experiment that resulted in the isolation of a series of rps2 and avrRpt2 alleles that disrupt the RPS2-avrRpt2 gene-for-gene interaction. Seven novel avrRpt2 alleles incapable of eliciting an RPS2-dependent HR all encode proteins with lesions in the C-terminal portion of AvrRpt2 previously shown to be sufficient for RPS2 recognition. Ten novel rps2 alleles were characterized with mutations in the NBS and the LRR. Several of these alleles code for point mutations in motifs that are conserved among NBS-LRR resistance genes, including the third LRR, which suggests the importance of these motifs for resistance gene function.

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