Bioreactor Design and Analysis for Large-Scale Plant Cell and Hairy Root Cultivation

Hairy Roots ◽  
2018 ◽  
pp. 147-182 ◽  
Author(s):  
Chitra Srikantan ◽  
Smita Srivastava
Keyword(s):  
Plant Cell ◽  
Hairy Root ◽  
Large Scale ◽  
Bioreactor Design

scholarly journals Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1610
Author(s):  
Branka Vinterhalter ◽  
Nevena Banjac ◽  
Dragan Vinterhalter ◽  
Dijana Krstić-Milošević
Keyword(s):  
Hairy Root ◽  
Large Scale ◽  
Bubble Column ◽  
Growth Parameters ◽  
Growth Index ◽  
Dry Weight ◽  
Growth Conditions ◽  
Good Growth ◽  
Gentiana Dinarica ◽  
Hairy Root Clones

The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin.

Enhanced production of falcarinol-type polyacetylenes in hairy roots of cultivated carrot (Daucus carota subsp. sativus)

2021 ◽  
Author(s):  
Frank Dunemann ◽  
Christoph Böttcher
Keyword(s):  
Hairy Root ◽  
Large Scale ◽  
Higher Plants ◽  
Leaf Tissue ◽  
Hairy Root Cultures ◽  
Scale Production ◽  
Root Cultures ◽  
Tissue Samples ◽  
Positive Effects ◽  
Enhanced Production

Abstract Polyacetylenes (PAs) are a large group of bioactive phytochemicals, which are primarily produced by higher plants of the families Apiaceae and Araliaceae. Especially aliphatic C17-polyacetylenes of the falcarinol-type such as falcarinol (FaOH) and falcarindiol (FaDOH) are known for their numerous positive effects on human health. In this study we investigate the potential of carrot hairy root cultures for production of PAs. Three individual plants of seven differently coloured carrot cultivars were used for the development of hairy root cultures by transformation of root discs with the wild-type Rhizobium rhizogenes strain 15834. A total of 51 individual hairy root (HR) lines were obtained and quantitatively analysed together with root, petiole and leaf tissue samples for FaOH and FaDOH. Among the five tissues sampled from the donor plants, root periderm samples generally exhibited the highest PA levels with FaDOH as prevailing PA and large differences between cultivars. In comparison to periderm tissue, FaOH levels were highly increased in HR lines of all cultivars. In contrast, FaDOH levels were not significantly altered. Considering the low to moderate PA concentration in root and leaf tissues of the orange cultivars there was an up to more than 10-fold increase of the FaOH concentration in HRs of these genotypes. Within this study a reproducible method for Rrhi-mediated transformation of carrot root discs was applied which provides an efficient tool to assess the function of candidate genes involved in the biosynthesis of key PAs in carrot but might be used in future also for the large-scale production of falcarinol-type PAs.

scholarly journals Methyl Jasmonate Induced Oxidative Stress and Accumulation of Secondary Metabolites in Plant Cell and Organ Cultures

2020 ◽  
Vol 21 (3) ◽  
pp. 716 ◽  
Author(s):  
Thanh-Tam Ho ◽  
Hosakatte Niranjana Murthy ◽  
So-Young Park
Keyword(s):  
Secondary Metabolites ◽  
Methyl Jasmonate ◽  
Plant Cell ◽  
Large Scale ◽  
Cultured Cells ◽  
Food Additives ◽  
Plant Secondary Metabolites ◽  
Signal Molecules ◽  
Scale Production ◽  
Organ Cultures

Recently, plant secondary metabolites are considered as important sources of pharmaceuticals, food additives, flavours, cosmetics, and other industrial products. The accumulation of secondary metabolites in plant cell and organ cultures often occurs when cultures are subjected to varied kinds of stresses including elicitors or signal molecules. Application of exogenous jasmonic acid (JA) and methyl jasmonate (MJ) is responsible for the induction of reactive oxygen species (ROS) and subsequent defence mechanisms in cultured cells and organs. It is also responsible for the induction of signal transduction, the expression of many defence genes followed by the accumulation of secondary metabolites. In this review, the application of exogenous MJ elicitation strategies on the induction of defence mechanism and secondary metabolite accumulation in cell and organ cultures is introduced and discussed. The information presented here is useful for efficient large-scale production of plant secondary metabolites by the plant cell and organ cultures.

scholarly journals Large-scale production of pharmaceutical proteins in plant cell culture-the protalix experience

2015 ◽  
Vol 13 (8) ◽  
pp. 1199-1208 ◽  
Author(s):  
Yoram Tekoah ◽  
Avidor Shulman ◽  
Tali Kizhner ◽  
Ilya Ruderfer ◽  
Liat Fux ◽  
...  
Keyword(s):  
Cell Culture ◽  
Plant Cell ◽  
Plant Cell Culture ◽  
Large Scale ◽  
Scale Production ◽  
Large Scale Production ◽  
Pharmaceutical Proteins

scholarly journals Plant cells technology as an effective biotechnological approach for high scale production of pharmaceutical natural compounds: A meta-analysis study

2019 ◽  
Author(s):  
Ali Davoodi ◽  
Elnaz Khoshvishkaie ◽  
Mohammad Azadbakht
Keyword(s):  
Medicinal Plants ◽  
Cell Suspension ◽  
Plant Cell ◽  
Hairy Root ◽  
Meta Analysis ◽  
Plant Cells ◽  
Scale Production ◽  
High Scale ◽  
Production Methods ◽  
Analysis Study

Natural-based drugs are the important bioactive substances that have been used for prevention and treatment of diseases. Natural products are prepared in commercial scale from relevant medicinal plants. Hence, large amounts of the plants are needed for extraction and isolation of naturally occurring compounds. Plant cells technology is the best strategy for the production of the plant-derived drugs, which have difficulty process in large scale production. This study was conducted for types, frequencies and efficacies of production methods for natural-based drugs in plant cell technology as an alternative method to preparation from whole herb. Pharmaceutical and biomedical databases including PubMed/Medline, Scopus, Web of Science, Embase, ProQuest and Google Scholar were searched in this study. Moreover, keywords words were ''secondary metabolite production'', ''pharmaceutical natural compounds'', ''high scale production'', ''cell suspension'', ''immobilized plant cell'', “hairy root”, ''elicitor'', ''substrate'', ''plant cell'', ''callus'', ''medicinal plants'', ''isolation and purification''. The correlations have been investigated by random effect model in an Excel program. Findings of this meta-analysis study showed all production methods had high efficacies and percentages of high scale production from 90 to 100%, which were comparable with conventional direct extractions. In addition to, median efficacy values for cell suspension, callus, hairy root and immobilized plant cell methods in production of selected drugs (atropine, paclitaxel, vincristine, camptothecin and colchicine) with 1124, 257, 797 and 969 events were 92.49 (CI95%: 89.78-95.86), 91.98 (CI95%: 89.13-95.25), 95.69 (CI95%: 92.84-98.68) and 93.86% (CI95%: 91.12-96.35), respectively. The plant cell technology for production of secondary metabolites has various advantages including high accuracy, repeatability and productivity, that is a best strategy for production of natural-based drugs.

Sign in / Sign up

Export Citation Format

Share Document