H-2 class I antigen expression on mouse teratocarcinoma cell lines

1985 ◽  
Vol 22 (6) ◽  
pp. 543-552 ◽  
Author(s):  
P. D�mant ◽  
M. Oudshoorn-Snoek
Keyword(s):  
Cell Lines ◽  
Antigen Expression ◽  
Class I ◽  
Teratocarcinoma Cell ◽  
Mouse Teratocarcinoma

scholarly journals Association of the H-Y male antigen with beta2-microglobulin on human lymphoid and differentiated mouse teratocarcinoma cell lines.

1978 ◽  
Vol 148 (1) ◽  
pp. 58-70 ◽  
Author(s):  
M Fellous ◽  
E Günther ◽  
R Kemler ◽  
J Wiels ◽  
R Berger ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Hla Antigens ◽  
Lymphoid Cell Line ◽  
Hla Antigen ◽  
Teratocarcinoma Cell ◽  
Daudi Cell ◽  
Human Male ◽  
Lymphoid Lines ◽  
Mouse Teratocarcinoma

The expression of the H-Y antigen has been tested on several human lymphoid lines and mouse teratocarcinoma cell lines during differentiation. The human male lymphoid cell line Raji is a very useful target for studies of the H-Y antigen by lymphocytotoxicity test with rat anti-H-Y sera. With a few exceptions, all cells carrying the Y chromosome were H-Y positive. One of the exceptions is the human Daudi cell line which, besides lacking H-Y antigen, also lacks beta2-microglobulin. We have studied a possible association between the H-Y antigen, beta2-microglobulin, and HLA antigen with redistribution experiments. The results strongly suggest that H-Y antigen is not associated with HLA antigens but with beta2-microglobulin.

scholarly journals High-molecular-weight glycoproteins are the major carriers of the carbohydrate differentiation antigens I, i and SSEA-1 of mouse teratocarcinoma cells

1983 ◽  
Vol 215 (3) ◽  
pp. 491-503 ◽  
Author(s):  
R A Childs ◽  
J Pennington ◽  
K Uemura ◽  
P Scudder ◽  
P N Goodfellow ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Cell Lines ◽  
Galactose Oxidase ◽  
Embryonal Carcinoma Cell ◽  
Teratocarcinoma Cell ◽  
Differentiation Antigens ◽  
Carcinoma Cell Lines ◽  
Teratocarcinoma Cells ◽  
Mouse Teratocarcinoma

The carriers of the carbohydrate differentiation antigens I, i and SSEA-1 were investigated in embryonal carcinoma cell lines of mouse and differentiated cell lines derived from them. Glycoproteins were studied by immunostaining (‘Western blotting’) of total cell lysates and immunoprecipitation from lysates of galactose oxidase/NaB3H4-labelled cells; glycolipids were investigated by immunostaining of thin layer chromatograms. The antigenic activities detected by immunofluorescence of cell smears were reflected in the antigenicities of high-molecular-weight glycoproteins. These were polydisperse and markedly susceptible to digestion with endo-beta-galactosidase. Only the I antigen was detected on minor glycolipids. These observations indicate that glycoproteins rather than glycolipids are the major carriers of carbohydrate differentiation antigens I, i and SSEA-1 in the teratocarcinoma cell lines.

Expression and modulation of major histocompatibility antigens on murine primary brain tumor in vitro

1991 ◽  
Vol 75 (6) ◽  
pp. 922-929 ◽  
Author(s):  
Aytac Akbasak ◽  
Edward H. Oldfield ◽  
Stephen C. Saris
Keyword(s):  
Major Histocompatibility Complex ◽  
Cell Lines ◽  
Antigen Expression ◽  
Class Ii ◽  
Gamma Interferon ◽  
Class I ◽  
Major Histocompatibility ◽  
Necrosis Factor Alpha ◽  
Histocompatibility Complex ◽  
Class Ii Antigen

✓ Lysis of tumor cells by activated cytotoxic lymphocytes requires their recognition of antigens associated with major histocompatibility complex molecules. The authors studied the constitutive expression of Class I and Class II major histocompatibility complex antigens on mouse brain-tumor cells and the capacity of different cytokines and cytokine combinations to alter this expression in vitro. Cells from the murine glioma 26 (GL26), glioma 261 (GL261), and ependymoblastoma A (EpA) cell lines were established in monolayer culture and treated for 48 hours with either alpha interferon, gamma interferon, tumor necrosis factor alpha, tumor necrosis factor alpha plus gamma interferon, or interleukin-2. They were then analyzed by flow cytometry for baseline and cytokine-altered major histocompatibility complex expression. All cell lines had a similar constitutive major histocompatibility complex pattern with low Class I antigen expression and no detectable Class II antigen expression. Alpha interferon substantially induced and up-regulated Class I antigen expression, but had no effect on Class II antigen expression. Gamma interferon also stimulated up-regulation of Class I antigen expression, generally doubling the anti-Class I antigen fluorescence of treated cells. Its effect on Class II antigen expression was more extensive. In the GL26 and GL261 cell lines, the expression of Class II antigen determinants increased to 12 × and 14 × control values and as many as 75% of cells that had no detectable constitutive expression of Class II antigen expressed this antigen after priming with gamma interferon. The addition of tumor necrosis factor alpha to gamma interferon further increased Class II antigen expression on EpA tumor cells only. Interleukin-2 and tumor necrosis factor alpha alone had no effect on Class I or Class II antigen expression of any cell lines. It is concluded that Class I and Class II antigen expression in mouse glioma cell lines is induced and enhanced after treatment with certain cytokines in vitro. Use of these cell lines to create in situ primary brain tumors in C57BL/6 mice should provide an excellent animal system to study major histocompatibility complex modulation in brain tumor cells and to examine the potential impact of major histocompatibility complex up-regulation on the response of brain tumors to immunotherapy.

scholarly journals Intensity of class I antigen expression on human tumour cell lines and its relevance to the efficiency of non-MHC-restricted killing

1993 ◽  
Vol 67 (6) ◽  
pp. 1223-1228 ◽  
Author(s):  
AME Nouri ◽  
RF Hussain ◽  
AVL Dos Santos ◽  
M Mansouri ◽  
RTD Oliver
Keyword(s):  
Cell Lines ◽  
Tumour Cell ◽  
Antigen Expression ◽  
Class I ◽  
Human Tumour ◽  
Tumour Cell Lines ◽  
Human Tumour Cell Lines

The comparison of cell surface HLA class I antigen expression with PCR-typed tumour cell lines by flow cytometry

1996 ◽  
Vol 47 (1-2) ◽  
pp. 25
Author(s):  
A Vora ◽  
A Murray ◽  
J Lawry ◽  
A Parker ◽  
R Start ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Surface ◽  
Cell Lines ◽  
Tumour Cell ◽  
Hla Class I ◽  
Antigen Expression ◽  
Class I ◽  
Hla Class I Antigen ◽  
Tumour Cell Lines

Natural killer susceptibility is independent of HLA class I antigen expression on cell lines obtained from human solid tumors

1990 ◽  
Vol 20 (11) ◽  
pp. 2445-2449 ◽  
Author(s):  
Jose Peña ◽  
Corona Alonso ◽  
Rafael Solana ◽  
Rafael Serrano ◽  
Julia Carracedo ◽  
...  
Keyword(s):  
Natural Killer ◽  
Solid Tumors ◽  
Cell Lines ◽  
Hla Class I ◽  
Antigen Expression ◽  
Class I ◽  
Hla Class I Antigen ◽  
Human Solid Tumors

Synthesis and secretion of β-nerve growth factor by mouse teratocarcinoma cell lines

1986 ◽  
Vol 167 (2) ◽  
pp. 287-294 ◽  
Author(s):  
Eleni Dicou ◽  
Rémi Houlgatte ◽  
Philippe Brachet
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cell Lines ◽  
Teratocarcinoma Cell ◽  
Nerve Growth ◽  
Mouse Teratocarcinoma
Sign in / Sign up

Export Citation Format

Share Document