scholarly journals Microtubule inhibitors block the morphological changes induced inDrosophila blood cells by a parasitoid wasp factor

1990 ◽  
Vol 46 (3) ◽  
pp. 311-315 ◽  
Author(s):  
R. M. Rizki ◽  
T. M. Rizki
Keyword(s):  
Blood Cells ◽  
Morphological Changes ◽  
Parasitoid Wasp ◽  
Microtubule Inhibitors

scholarly journals An Evaluation of Morphological Changes and Deformability of Suspended Red Blood Cells Prepared Using Whole Blood with Different Hemoglobin Levels of Tibetans

2021 ◽  
pp. 1-10
Author(s):  
Rui Zhong ◽  
Dingding Han ◽  
Xiaodong Wu ◽  
Hong Wang ◽  
Wanjing Li ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Morphological Changes ◽  
Osmotic Fragility ◽  
Spherical Shape ◽  
Hypoxic Environment ◽  
Wide Range ◽  
Group A ◽  
Cell Membrane Protein

Background: The hypoxic environment stimulates the human body to increase the levels of hemoglobin (HGB) and hematocrit and the number of red blood cells. Such enhancements have individual differences, leading to a wide range of HGB in Tibetans’ whole blood (WB). Study Design: WB of male Tibetans was divided into 3 groups according to different HGB (i.e., A: >120 but ≤185 g/L, B: >185 but ≤210 g/L, and C: >210 g/L). Suspended red blood cells (SRBC) processed by collected WB and stored in standard conditions were examined aseptically on days 1, 14, 21, and 35 after storage. The routine biochemical indexes, deformability, cell morphology, and membrane proteins were tested. Results: Mean corpuscular volume, adenosine triphosphate, pH, and deformability were not different in group A vs. those in storage (p > 0.05). The increased rate of irreversible morphology of red blood cells was different among the 3 groups, but there was no difference in the percentage of red blood cells with an irreversible morphology after 35 days of storage. Group C performed better in terms of osmotic fragility and showed a lower rigid index than group A. Furthermore, SDS-PAGE revealed similar cross-linking degrees of cell membrane protein but the band 3 protein of group C seemed to experience weaker clustering than that of group A as detected by Western Blot analysis after 35 days of storage. Conclusions: There was no difference in deformability or morphological changes in the 3 groups over the 35 days of storage. High HGB levels of plateau SRBC did not accelerate the RBC change from a biconcave disc into a spherical shape and it did not cause a reduction in deformability during 35 days of preservation in bank conditions.

scholarly journals Hemoglobin Oxidation in Stored Blood Accelerates Hemolysis and Oxidative Injury to Red Blood Cells

2020 ◽  
Vol 12 (04) ◽  
pp. 244-249
Author(s):  
Ibrahim Mustafa ◽  
Tameem Ali Qaid Hadwan
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Morphological Changes ◽  
Hemoglobin Concentration ◽  
Red Cell Membrane ◽  
Rbc Membrane ◽  
Hemoglobin Oxidation ◽  
Rbc Indices ◽  
Methemoglobin Formation ◽  
Stored Blood

Abstract Introduction Maintaining blood supply is a challenge in blood banks. Red blood cells (RBCs) stored at 4°C experience issues of biochemical changes due to metabolism of cells, leading to changes collectively referred to as “storage lesions.” Oxidation of the red cell membrane, leading to lysis, contributes to these storage lesions. Methods Blood bags with CPD-SAGM stored at 4°C for 28 days were withdrawn aseptically on days 1, 14, and 28. Hematology analyzer was used to investigate RBC indices. Hemoglobin oxidation was studied through spectrophotometric scan of spectral change. RBC lysis was studied with the help of Drabkin's assay, and morphological changes were observed by light and scan electron microscopy. Results RBCs show progressive changes in morphology echinocytes and spherocytes on day 28. There was 0.85% RBC lysis, an approximately 20% decrease in percentage oxyhemoglobin, and a 14% increase in methemoglobin formation, which shows hemoglobin oxidation on day 28. Conclusions Oxidative damage to RBC, with an increase in storage time was observed in the present study. The observed morphological changes to RBC during the course of increased time shows that there is progressive damage to RBC membrane and a decrease in hemoglobin concentration; percentage RBC lysis is probably due to free hemoglobin and iron.

Morphological changes of the red blood cells treated with metal oxide nanoparticles

2016 ◽  
Vol 37 ◽  
pp. 34-40 ◽  
Author(s):  
A.I. Kozelskaya ◽  
A.V. Panin ◽  
I.A. Khlusov ◽  
P.V. Mokrushnikov ◽  
B.N. Zaitsev ◽  
...  
Keyword(s):  
Metal Oxide ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Morphological Changes ◽  
Metal Oxide Nanoparticles ◽  
Oxide Nanoparticles

Cryopreservation of feline red blood cells in liquid nitrogen using glycerol and hydroxyethyl starch

2019 ◽  
Vol 22 (4) ◽  
pp. 366-375
Author(s):  
Marcelle Hon ◽  
Elizabeth J Thomovsky ◽  
Aimee C Brooks ◽  
Paula A Johnson
Keyword(s):  
Red Blood Cells ◽  
Liquid Nitrogen ◽  
Hydroxyethyl Starch ◽  
Blood Cells ◽  
Recovery Rate ◽  
Morphological Changes ◽  
High Viscosity ◽  
Washing Process ◽  
Handling Technique ◽  
Packed Rbcs

ObjectivesThe objective of this study was to evaluate the techniques and short-term effects of cryopreservation of feline red blood cells (RBCs) in liquid nitrogen using glycerol or hydroxyethyl starch (HES) as a cryoprotectant.MethodsFeline RBCs were manually mixed with either 20% glycerol or 12.5% HES and frozen for 24 h in liquid nitrogen. The samples were thawed and glycerolized samples were manually washed. Success of the freeze/thaw process was determined by recovery rate of RBCs and evaluation of morphological changes using scanning electron microscopy (SEM). A unit of canine packed RBCs was also subjected to the same methodology to evaluate the cryopreservation handling technique.ResultsFeline RBCs preserved with 20% glycerol had a high recovery rate (94.23 ± 1.25%) immediately after thawing. However, the majority of the cells were lost during the washing process, with a final packed cell volume of <1%. A recovery rate was unable to be assessed for samples preserved with HES owing to the high viscosity of the mixture. SEM revealed significant morphological changes after glycerol was added to the feline RBCs. Although these morphological changes were partially reversed after thawing, the majority of the RBCs were lost during the washing process. Minimal morphological changes were noted in the HES sample. Similar results were noted with the canine RBCs.Conclusions and relevanceThe described manual technique for cryopreservation using glycerol was not able to successfully preserve feline or canine RBCs. In the present study, it was difficult to make conclusions about the efficacy of HES. Further studies evaluating HES as a cryoprotectant are warranted.

Morphological Changes of Red Blood Cells in Peripheral Blood Smear of Patients with Pregnancy-related Hypertensive Disorders

2015 ◽  
Vol 46 (6) ◽  
pp. 479-483 ◽  
Author(s):  
Jorge Daniel Hernández Hernández ◽  
Onésimo Rangel Villaseñor ◽  
Javier Del Rio Alvarado ◽  
Roberto Ortega Lucach ◽  
Arturo Zárate ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Blood Smear ◽  
Morphological Changes ◽  
Peripheral Blood Smear ◽  
Hypertensive Disorders

scholarly journals The improvement of blood smears coloring for the determining of fish cytometric blood parameters

2019 ◽  
Vol 11 (1) ◽  
pp. 15-18
Author(s):  
V Kurchenko ◽  
T Sharamok ◽  
O Marenkov
Keyword(s):  
Blood Cells ◽  
Morphological Changes ◽  
Blood Parameters ◽  
Field Conditions ◽  
Improved Method ◽  
Fish Blood ◽  
Blood Smears ◽  
Correct Form ◽  
The Subject

Fish erythrocytes are sensitive to environmental pollution and their morphological changes can be used as a bioindicator of the toxicity of the aquatic environment. The disadvantage of existing methods of blood smears coloring is that they are inconvenient in the field conditions, when it is necessary to examine the fish blood during ichthyological researches or they are long-lasting. In this regard, there is a need for a fast and reliable method of coloring blood cells of fish, which can be used both in laboratory and in field conditions. The aim of the work was to improve the method of coloring fish blood smears for easier and faster execution, without degradation of the quality of colouring. The subject of the research was the young Cyprinidae fish. The improved method is based on the classic method of painting by Papenheim, but differs in the interval of time devoted to colouring in a solution of the dye Romanovskogo. The analysis showed that erythrocytes on medicine that were in the Romanovsky solution for 5 minutes had the correct form with clear contours, a dark purple core located in the center of the cell. It has been found that this method can be used in cytometric researches to determine such indicators: large longitudinal and small transverse diameters of erythrocytes, erythrocyte area, erythrocyte core area, nuclear-cytoplasmic ratio, percentage of mature and immature erythrocytes and their forms. It can also be used in analyzing the presence of erythrocyte pathologies. It has been established that reduction of time (from 20 minutes to 5 minutes) of preparations in the dye solution of Romanovsky does not affect the quality of their coloring, and therefore it is a reliable and fast way that can be successfully applied in morphological and cytometric studies of blood of fish.

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