Detection of Anti-HTLV-III/IAV antibody by enzyme-linked immunosorbent assay in high-risk individuals in Switzerland 1974–1985

1985 ◽  
Vol 4 (6) ◽  
pp. 583-586 ◽  
Author(s):  
J. Stroun ◽  
I. Graf ◽  
P. C. Frei
Keyword(s):  
High Risk ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

Quantitative measurement of anti-aquaporin-4 antibodies by enzyme-linked immunosorbent assay using purified recombinant human aquaporin-4

2011 ◽  
Vol 18 (5) ◽  
pp. 578-586 ◽  
Author(s):  
Woojun Kim ◽  
Ji-Eun Lee ◽  
Xue Feng Li ◽  
Su-Hyun Kim ◽  
Byeong-Gu Han ◽  
...  
Keyword(s):  
High Risk ◽  
Disease Activity ◽  
Immunosorbent Assay ◽  
Neurological Diseases ◽  
Aquaporin 4 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Marker ◽  
Longitudinal Measurement ◽  
Longitudinal Measurements ◽  
Baculovirus System

Background: Antibodies to aquaporin-4 (AQP4-Ab), known as NMO-IgG, are a sensitive and specific marker for neuromyelitis optica (NMO). Methods: To develop an enzyme-linked immunosorbent assay (ELISA) for AQP4-Ab, we expressed M23 isoform of human AQP4 in a baculovirus system, and used it as an antigen. We measured AQP4-Ab in the sera of 300 individuals: 64 with definite NMO, 31 with high-risk NMO, 105 with multiple sclerosis (MS), 57 with other neurological diseases (ONDs), and 43 healthy controls. We also performed longitudinal measurements of AQP4–Ab in 787 samples collected from 51 patients with definite or high-risk NMO. Results: AQP4-Abs were positive in 72% with definite NMO, 55% with high-risk NMO, and 4% with MS, but none of the OND patients and the healthy individuals. The longitudinal measurement showed AQP4-Ab levels correlating with disease activity. Out of 38 initially seropositive patients, 21 became seronegative under effective immunosuppressive therapy. During most relapses, the serum AQP4-Ab levels were either high or rising compared with the previous value, although rising AQP4-Ab levels did not always lead to acute exacerbation. Two of the 13 initially seronegative patients converted to seropositive following acute exacerbations. Conclusions: We established an AQP4-Ab ELISA, which could be a potential monitoring tool of disease activity.

scholarly journals Estimation of malaria transmission in high-risk provinces of Morocco

2003 ◽  
Vol 9 (4) ◽  
pp. 542-547
Author(s):  
C. Faraj ◽  
E. Adlaoui ◽  
M. Rhajaoui ◽  
M. Lyagoubi
Keyword(s):  
High Risk ◽  
Plasmodium Vivax ◽  
Malaria Transmission ◽  
Immunosorbent Assay ◽  
Epidemiological Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Light Traps ◽  
Human Malaria ◽  
Mosquito Collection ◽  
Transmission Level

The malaria transmission level of Plasmodium vivax was monitored in four high-risk provinces in Morocco. Intensive mosquito collection by light traps and manual catches resulted in the capture of four species: Anopheles labranchiae, An. sergenti, An. cinereus, and An. claviger. All An. sergenti and An. labranchiae females collected were tested for the presence of two phenotypes of P. vivax [PVK210 and PVK247] antigen by enzyme-linked immunosorbent assay [ELISA]. No P. vivax antigen was detected in 1347 mosquitoes analysed. A parallel parasitological investigation was conducted. Of 2665 slides examined from a population of 4343 people for detection of P. vivax, no slide was positive. The results confirm the break in malaria transmission in residual foci. The use of ELISA is recommended in future epidemiological studies of human malaria.

Detection of anti-HTLV-I Tax antibodies in HTLV-I enzyme-linked immunosorbent assay-negative individuals

Blood ◽  
1989 ◽  
Vol 74 (3) ◽  
pp. 1066-1072 ◽  
Author(s):  
GD Ehrlich ◽  
JB Glaser ◽  
MA Abbott ◽  
DJ Slamon ◽  
D Keith ◽  
...  
Keyword(s):  
High Risk ◽  
Immunosorbent Assay ◽  
Risk Groups ◽  
Enzyme Linked Immunosorbent Assay ◽  
Drug Abusers ◽  
Western Blots ◽  
True Incidence ◽  
Tax Protein ◽  
Radioimmunoprecipitation Assay

Abstract The HTLV-I tax gene protein (Tax) is not packaged within the mature viral particle from which the proteins for the commercially available enzyme-linked immunosorbent assay (ELISA) are derived. Screening of 162 individuals within a cohort of white intravenous (IV) drug abusers, previously identified as having an increased incidence of HTLV-I infection, demonstrated that seven of them had antibodies to the HTLV-I Tax protein but tested negative in HTLV-I ELISAs and Western blots prepared from purified virion proteins. Three out of 35 individuals in other behaviorally defined high-risk groups also displayed this limited pattern of reactivity to HTLV-I proteins. The presence of the anti-HTLV- I p40/Tax antibodies was determined by radioimmunoprecipitation assay (RIPA), which also revealed low levels of anti-env reactivity. The specificity of the anti-p40 reactivity was confirmed on specific Tax ELISAs and Western blots prepared from recombinantly produced Tax. In vitro gene amplification by the polymerase chain reaction (PCR) was used to establish the presence of sequences homologous to HTLV-I proviral DNA in four/four of these HTLV-I ELISA negative, Tax ELISA/Tax western blot/RIPA positive individuals. These data suggest that the true incidence of HTLV-I infection within high-risk cohorts is greater than previously reported.

scholarly journals Direct Human Papillomavirus E6 Whole-Cell Enzyme-Linked Immunosorbent Assay for Objective Measurement of E6 Oncoproteins in Cytology Samples

2012 ◽  
Vol 19 (9) ◽  
pp. 1474-1479 ◽  
Author(s):  
Yi-Shan Yang ◽  
Karen Smith-McCune ◽  
Teresa M. Darragh ◽  
Yvonne Lai ◽  
Ju-Hwa Lin ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Immunosorbent Assay ◽  
Objective Measurement ◽  
Enzyme Linked Immunosorbent Assay ◽  
Whole Cell ◽  
Hpv Dna ◽  
Hpv E6 ◽  
Cell Enzyme ◽  
Grade 3

ABSTRACTA novel, whole-cell enzyme-linked immunosorbent assay (ELISA) based on a non-type-specific anti-human papillomavirus (HPV) E6 antibody was tested on 182 residual cytological specimens. For samples with a designation of more severe thancervicalintraepithelialneoplasia grade 3 (CIN3+), 83% tested positive for E6; in a subset with paired testing for E6 ELISA and HPV DNA, 72% tested E6 positive and 92% tested high-risk (HR)-HPV DNA positive (P= 0.2). Among the women with a less than CIN3 diagnosis, 31% and 47% tested positive for E6 and HR-HPV DNA, respectively (P= 0.0006).

scholarly journals Detection of anti-HTLV-I Tax antibodies in HTLV-I enzyme-linked immunosorbent assay-negative individuals

Blood ◽  
1989 ◽  
Vol 74 (3) ◽  
pp. 1066-1072
Author(s):  
GD Ehrlich ◽  
JB Glaser ◽  
MA Abbott ◽  
DJ Slamon ◽  
D Keith ◽  
...  
Keyword(s):  
High Risk ◽  
Immunosorbent Assay ◽  
Risk Groups ◽  
Enzyme Linked Immunosorbent Assay ◽  
Drug Abusers ◽  
Western Blots ◽  
True Incidence ◽  
Tax Protein ◽  
Radioimmunoprecipitation Assay

The HTLV-I tax gene protein (Tax) is not packaged within the mature viral particle from which the proteins for the commercially available enzyme-linked immunosorbent assay (ELISA) are derived. Screening of 162 individuals within a cohort of white intravenous (IV) drug abusers, previously identified as having an increased incidence of HTLV-I infection, demonstrated that seven of them had antibodies to the HTLV-I Tax protein but tested negative in HTLV-I ELISAs and Western blots prepared from purified virion proteins. Three out of 35 individuals in other behaviorally defined high-risk groups also displayed this limited pattern of reactivity to HTLV-I proteins. The presence of the anti-HTLV- I p40/Tax antibodies was determined by radioimmunoprecipitation assay (RIPA), which also revealed low levels of anti-env reactivity. The specificity of the anti-p40 reactivity was confirmed on specific Tax ELISAs and Western blots prepared from recombinantly produced Tax. In vitro gene amplification by the polymerase chain reaction (PCR) was used to establish the presence of sequences homologous to HTLV-I proviral DNA in four/four of these HTLV-I ELISA negative, Tax ELISA/Tax western blot/RIPA positive individuals. These data suggest that the true incidence of HTLV-I infection within high-risk cohorts is greater than previously reported.

scholarly journals Terapi Hipotermia Ringan Menurunkan Kadar Protein MMP-9 dan Memperbaiki FOUR Score pada Cedera Otak Traumatik Risiko Tinggi

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Hendra . ◽  
Halim Wijaya ◽  
Eko Prasetyo ◽  
Maximillian Ch. Oley
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
High Risk ◽  
Therapeutic Hypothermia ◽  
Immunosorbent Assay ◽  
Neurological Outcome ◽  
Mild Hypothermia ◽  
Mild Therapeutic Hypothermia ◽  
Enzyme Linked Immunosorbent Assay ◽  
Four Score

Abstract: In this study, patients with high risk traumatic brain injury (TBI) were treated with mild therapeutic hypothermia and their serum MMP-9 levels as well as neurological outcome were evaluated. This was an experimental analytical study conducted at Intensive Care Unit Prof. Dr. R. D. Kandou Hospital, Manado. The results showed that there were 20 patients with high risk TBI (the FOUR score <7) prospective randomly assigned to mild therapeutic hypothermia and control groups. Patients were evaluated at the time of admission and after 72 hours. The serum MMP-9 level was examined by using enzyme-linked immunosorbent assay (ELISA) and the clinical outcome was evaluated by using the FOUR score. Levels of the variables were compared between the two groups. In the hypothermia group, level of serum MMP-9 was decreased (P > 0.05) after 72 hours and there was a significant improvement of the FOUR score (P < 0.05). Conclusion: Mild therapeutic hypothermia could reduce serum MMP-9 level and significantly improve the neurological outcome after 72 hours.Keyword: mild hypothermia, MMP-9, FOUR scoreAbstrak: Penelitian ini dilakukan terhadap penderita cedera otak akibat trauma (COT) risiko tinggi yang diberi perlakuan hipotermia ringan (HPTr) dengan mengevaluasi kadar MMP-9 serum dan hasil klinis yang dinilai melalui skor FOUR. Jenis penelitian ini ialah eksperimental analitik. Penelitian ini dilakukan di Intensive Care Unit (ICU) RSUP Prof. Dr. R. D. Kandou Manado. Terdapat total 20 pasien dengan COT risiko tinggi (skor FOUR <7) yang secara random dibagi dalam kelompok kontrol dan kelompok HPTr. Pasien dinilai saat masuk dan setelah 72 jam kemudian. Kadar MMP-9 serum dinilai menggunakan enzyme-linked immunosorbent assay (ELISA). Perbedaan level dari kedua variabel ini dibandingkan pada kedua kelompok. Hasil penelitian memperlihatkan bahwa pada kelompok perlakuan HPTr, kadar MMP-9 serum berkurang setelah 72 jam walaupun tidak bermakna (P > 0,05) dan terdapat peningkatan skor FOUR secara bermakna (P < 0,05). Simpulan: Perlakuan hipotermi ringan dapat menurunkan kadar MMP-9 serum dan memberikan peningkatan hasil klinis melalui penilaian skor FOUR setelah 72 jam kemudian.Kata kunci: hipotermi ringan, MMP-9, FOUR score

Hepatitis A immunisation need in nursing students in Turkey

2018 ◽  
Vol 48 (3) ◽  
pp. 221-224
Author(s):  
Çiğdem Kader ◽  
Ayşe Erbay ◽  
Nazan Kılıç Akça ◽  
Selda Yüzer Alsaç ◽  
Sevinç Polat
Keyword(s):  
Nursing Students ◽  
High Risk ◽  
Immunosorbent Assay ◽  
Immunoglobulin G ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
First Year ◽  
Serological Status ◽  
Preventable Infection

Hepatitis A is a worldwide vaccine-preventable infection. The aim of our study was to determine the serological status of hepatitis A virus (HAV) among first-year nursing students in Turkey. A sample of 423 students was used and immunoglobulin G antibodies against HAV were determined quantitatively by enzyme-linked immunosorbent assay on each. Overall, 84.6% had no immunity to HAV, making them at risk for HAV, and so susceptible to nosocomial transmission. Nursing students who work in high-risk wards must be vaccinated against hepatitis A.

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